期刊
BRITISH JOURNAL OF NUTRITION
卷 107, 期 9, 页码 1305-1315出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0007114511004387
关键词
n-3 PUFA; Dyslipidaemia; Glucose intolerance; Endothelial function; Hamsters
资金
- CRNH (Centre de Recherche en Nutrition Humaine, Nantes, France)
- Region Pays de la Loire
Glucose intolerance and dyslipidaemia are independent risk factors for endothelium dysfunction and CVD. The aim of the present study was to analyse the preventive effect of n-3 PUFA (EPA and DHA) on lipid and carbohydrate disturbances and endothelial dysfunction. Three groups of adult hamsters were studied for 20 weeks: (1) control diet (Control); (2) high-fat diet (HF); (3) high-fat diet enriched with n-3 PUFA (HFn-3) groups. The increase in body weight and fat mass in the HF compared to the Control group (P<0.05) was not found in the HFn-3 group. Muscle TAG content was similar in the Control and HF groups, but significantly lower in the HFn-3 group (P=0.008). Glucose tolerance was impaired in the HF compared to the Control group, but this impairment was prevented by n-3 PUFA in the HFn-3 group (P<0.001). Plasma TAG and cholesterol were higher in the HF group compared to the Control group (P<0.001), but lower in the HFn-3 group compared to the HF group (P<0.001). HDL-cholesterol was lower in the HFn-3 group compared to the Control and HF groups (P<0.0005). Hepatic secretion of TAG was lower in the HFn-3 group compared to the HF group (P<0.005), but did not differ from the Control group. Hepatic gene expression of sterol regulatory element-binding protein-1c, diacylglycerol O-acyltransferase 2 and stearyl CoA desaturase 1 was lower in the HFn-3 group, whereas carnitine palmitoyl transferase 1 and scavenger receptor class B type 1 expression was higher (P<0.05). In adipocytes and adipose macrophages, PPAR gamma and TNF alpha expression was higher in the HF and HFn-3 groups compared to the Control group. Endothelium relaxation was higher in the HFn-3 (P<0.001) than in the HF and Control groups, and was correlated with glucose intolerance (P=0.03) and cholesterol (P=0.0003). In conclusion, n-3 PUFA prevent some metabolic disturbances induced by high-fat diet and improve endothelial function in hamsters.
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