期刊
BRITISH JOURNAL OF NUTRITION
卷 104, 期 8, 页码 1148-1155出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0007114510001820
关键词
Ca2+ handling; Insulin secretion; Protein kinase A; Phospholipase C; Taurine supplementation
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP [2008/53811-8, 2007/50365-4, 2005/59707-0]
- Conselho Nacional para o Desenvolvimento Cientifico e Tecnologico (CNPq)
- Instituto Nacional de Obesidade e Diabetes (CNPq/FAPESP)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
Taurine (TAU) supplementation increases insulin secretion in response to high glucose concentrations in rodent islets. This effect is probably due to an increase in Ca2+ handling by the islet cells. Here, we investigated the possible involvement of the cholinergic/phospholipase C (PLC) and protein kinase (PK) A pathways in this process. Adult mice were fed with 2% TAU in drinking water for 30 d. The mice were killed and pancreatic islets isolated by the collagenase method. Islets from TAU-supplemented mice showed higher insulin secretion in the presence of 8.3 mM-glucose, 100 mu M-carbachol (Cch) and 1 mM-3-isobutyl-1-methyl-xanthine (IBMX), respectively. The increase in insulin secretion in response to Cch in TAU islets was accompanied by a higher intracellular Ca2+ mobilisation and PLC beta 2 protein expression. The Ca2+ uptake was higher in TAU islets in the presence of 8.3 mM-glucose, but similar when the islets were challenged by glucose plus IBMX. TAU islets also showed an increase in the expression of PKA alpha protein. This protein may play a role in cation accumulation, since the amount of Ca2+ in these islets was significantly reduced by the PKA inhibitors: N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinoline sulfonamide (H89) and PK inhibitor-(6-22)-amide (PKI). In conclusion, TAU supplementation increases insulin secretion in response to glucose, favouring both influx and internal mobilisation of Ca2+, and these effects seem to involve the activation of both PLC-inositol-1,4,5-trisphosphate and cAMP-PKA pathways.
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