Article
Food Science & Technology
Takamasa Masuda, Yoshinaka Ino, Akiko Hirai, Akiko Okamura, Hirohito Ishikawa, Shin-ichiro Yokoyama, Toshihiko Osawa
Summary: The study suggests that daily intake of red clover can reduce blood glucose, especially for individuals aged 50 and under. Formononetin, an alpha-glucosidase inhibitor, is considered to be the major functional molecule for these effects.
JOURNAL OF FOOD SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Anna Gosciniak, Piotr Szulc, Waldemar Zielewicz, Jaroslaw Walkowiak, Judyta Cielecka-Piontek
Summary: Red clover, a raw material with isoflavone content, was studied to evaluate the properties of its leaves and flowers. The study found that the ability to inhibit α-glucosidase, lipase, collagenase and antioxidant activity was not correlated with isoflavone content.
Article
Agriculture, Dairy & Animal Science
Jennifer R. Weinert-Nelson, Donald G. Ely, Michael D. Flythe, Tracy A. Hamilton, John B. May, Jessica L. Ferrell, Matthew C. Hamilton, Whitney LeeAnn Jacks, Brittany E. Davis
Summary: The goal of this study was to evaluate the effects of biochanin A supplementation through red clover hay on the growth performance and rumen microbiota of ram lambs. Low levels of red clover hay inclusion in high-concentrate finishing diets improved feed efficiency and weight gain while decreasing feed intake. Red clover supplementation also altered the rumen microbiota and promoted fiber utilization.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Plant Sciences
Stefano Zanotto, Annick Bertrand, Randy W. Purves, Jorunn E. Olsen, Fatma M. Elessawy, Ashild Ergon
Summary: The ability to tolerate low freezing temperatures is crucial for the survival and persistence of red clover in winter. In this study, the effect of cold acclimation (CA) on the contents of carbohydrates, amino acids, and phenolic compounds in red clover crowns was investigated. The results showed that freezing tolerant (FT) accessions had higher levels of certain compounds, including raffinose, pinitol, arginine, serine, alanine, valine, phenylalanine, and a pinocembrin hexoside derivative, compared to freezing susceptible (FS) accessions. These findings provide new insights into the biochemical changes during cold acclimation and their role in freezing tolerance in Nordic red clover.
PHYSIOLOGIA PLANTARUM
(2023)
Review
Food Science & Technology
Saied A. Aboushanab, Shaimaa M. Khedr, Irina F. Gette, Irina G. Danilova, Natalia A. Kolberg, Gokare A. Ravishankar, Ranga Rao Ambati, Elena G. Kovaleva
Summary: Isoflavones, the most abundant category of plant polyphenols, have demonstrated health benefits but studies remain variable and controversial, requiring further standardized analysis to explore their potential therapeutic effects.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Oncology
Minami Hatono, Hirokuni Ikeda, Yoko Suzuki, Yukiko Kajiwara, Kengo Kawada, Takahiro Tsukioki, Mariko Kochi, Ken Suzawa, Takayuki Iwamoto, Hiromasa Yamamoto, Tadahiko Shien, Masaomi Yamane, Naruto Taira, Hiroyoshi Doihara, Shinichi Toyooka
Summary: Isoflavones metabolite equol has dual effects on breast cancer, promoting tumor growth at low concentrations in hormone receptor-positive cells and exhibiting antitumor effects at high doses. When used with tamoxifen, equol may have antagonistic effects depending on concentration, but does not show antagonistic effects with other therapeutic agents.
BREAST CANCER RESEARCH AND TREATMENT
(2021)
Article
Agriculture, Dairy & Animal Science
V. Tosar, G. Rousseau, V. Decruyenaere, Y. Beckers, Y. Larondelle, E. Froidmont
Summary: This study evaluated the production of equol-enriched eggs from free-range hens fed different pasture species, with red clover showing the highest concentrations of daidzein and formononetin in fresh pasture samples, resulting in equol concentrations of about 1,200 ng/g DM in eggs from hens with access to red clover, making these eggs a valuable source of equol in the human diet.
BRITISH POULTRY SCIENCE
(2021)
Article
Nutrition & Dietetics
Annemarie B. Villadsen, Julie N. Holm-Jacobsen, Bala K. Prabhala, Caspar Bundgaard-Nielsen, Pam Huntjens, Jette B. Kornum, Karin Glavind, Peter D. C. Leutscher, Lars P. Christensen, Per B. Jeppesen, Suzette Sorensen, Louise T. S. Arenholt
Summary: This study investigates the potential of fermented red clover isoflavones to reduce overactive bladder (OAB) symptoms in postmenopausal women. The results showed that intake of red clover extract did not significantly relieve most urinary bladder symptom measures, although it did show a significant reduction in the bother of urinary urgency and a tendency towards a decreased ICIQ-OAB score.
Article
Plant Sciences
Randy D. Dinkins, Julie Hancock, Brenda L. Coe, John B. May, Jack P. Goodman, William T. Bass, Jinge Liu, Yinglun Fan, Qiaolin Zheng, Hongyan Zhu
Summary: This study found that isoflavones in red clover do not play a key role in rhizobial signaling but may be involved in defense in the rhizosphere. Knocking out the key enzyme in isoflavone biosynthesis using CRISPR/Cas9 resulted in significantly reduced levels of isoflavones in mutant plants. Analysis of gene expression profiles suggested that the mutant plants might have been responding to cues in the surrounding rhizosphere due to lower levels of isoflavones.
PLANT CELL REPORTS
(2021)
Review
Food Science & Technology
Susana Langa, Angela Peiroten, Jose Antonio Curiel, Ana Ruiz De la Bastida, Jose Maria Landete
Summary: Isoflavones, a type of phenolic compounds known as phytoestrogens, have estrogenic and antioxidant functions that are highly beneficial for human health, particularly in the elderly. However, isoflavones in foods have low bioavailability and need to be transformed by microorganisms to enhance their beneficial effects. Lactic acid bacteria (LAB) can naturally convert isoflavones in foods into more bioavailable forms, and certain LAB strains can further convert isoflavones into compounds with greater biological activity. This article will discuss the metabolism of isoflavones by LAB and their potential application in the development of foods enriched in bioactive isoflavones, as well as the associated health benefits.
Article
Biochemical Research Methods
LeNaiya Kydd, Priyanka Shiveshwarkar, Justyn Jaworski
Summary: Introducing metabolic pathways to the gut is important for regulating the biochemical components absorbed by the host. Different gut bacteria can lead to distinct health outcomes, and engineering bacteria to produce enzymes can achieve artificial metabolism of isoflavones.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Geng Hu, Liying Liu, Xiuxiu Miao, Yanan Zhao, Yanan Peng, Xianyao Li
Summary: This study utilized reciprocal crosses of two China local chicken breeds to investigate the mechanism of avian resistance to S. Enteritidis. The findings highlighted the role of 12 species of microbes in enhancing intestinal stability through the production of phenylpropanoids.
MICROBIAL BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sandra Yu, Francesco Caruso, Stuart Belli, Miriam Rossi
Summary: This study evaluates the biological role of isoflavones, including formononetin (FMNT), in antioxidant and superoxide reduction activity, as well as their potential PPAR gamma agonist activity. The findings provide insights for further research on the therapeutic potential of natural products in diabetes treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Kenneth D. R. Setchell, Evangelia Mourvaki, Carlo Clerici, Simona Mattioli, Gabriele Brecchia, Cesare Castellini
Summary: This study compared the effects of dietary isoflavone aglycones and glycoside conjugates in aging hens, finding that isoflavone aglycones improved egg-laying efficiency, decreased plasma cholesterol concentrations, and reduced yolk cholesterol content in eggs.
Article
Chemistry, Applied
Maria Teresa Munoz, Vanessa Maldonado, Wence Herrera, Ana Mutis, Leonardo Bardehle, Cristian Medina, Emilio Hormazabal, Fernando Ortega, Andres Quiroz
Summary: The study aimed to determine the optimal conditions for isoflavone synthase activity through a response surface model and evaluate the formononetin content in Trifolium pratense. The results indicated that the best enzymatic assay condition was at pH 9.1 with an incubation at 34.5 degrees C for 155 minutes, with Precoz-3, Precoz-1, and Superqueli-INIA showing the highest production levels.
NATURAL PRODUCT RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Zydrune Polianskyte-Prause, Tuomas A. Tolvanen, Sonja Lindfors, Vincent Dumont, Mervi Van, Hong Wang, Surjya N. Dash, Mika Berg, Jette-Britt Naams, Laura C. Hautala, Harry Nisen, Tuomas Mirtti, Per-Henrik Groop, Kristiina Wahala, Jukka Tienari, Sanna Lehtonen
Article
Biochemistry & Molecular Biology
Jenni Tienaho, Maarit Karonen, Riina Muilu-Makela, Kristiina Wahala, Eduardo Leon Denegri, Robert Franzen, Matti Karp, Ville Santala, Tytti Sarjala
Article
Biochemistry & Molecular Biology
Phillip Round, Samir Das, Tsung-Sheng Wu, Kristiina Wahala, Filip Van Petegem, Geoffrey L. Hammond
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Mika E. A. Berg, Jette-Britt Naams, Laura C. Hautala, Tuomas A. Tolvanen, Jari P. Ahonen, Sanna Lehtonen, Kristiina Wahala
Article
Biochemistry & Molecular Biology
Sofia Otaru, Surachet Imlimthan, Mirkka Sarparanta, Kerttuli Helariutta, Kristiina Wahala, Anu J. Airaksinen
Article
Agriculture, Dairy & Animal Science
T. Stefanski, S. Ahvenjarvi, A. Vanhatalo, P. Huhtanen
JOURNAL OF DAIRY SCIENCE
(2020)
Article
Plant Sciences
Jenni Tienaho, Maarit Karonen, Riina Muilu-Makela, Janne Kaseva, Nuria de Pedro, Francisca Vicente, Olga Genilloud, Ulla Aapola, Hannu Uusitalo, Katriina Vuolteenaho, Robert Franzen, Kristiina Wahala, Matti Karp, Ville Santala, Tytti Sarjala
Article
Biochemistry & Molecular Biology
Harri Latva-Maenpaa, Riziwanguli Wufu, Daniel Mulat, Tytti Sarjala, Pekka Saranpaa, Kristiina Wahala
Summary: Stilbenes, major polyphenolic compounds of the bark of Norway spruce, have potential applications as drugs, preservatives, and other functional ingredients due to their antioxidative, antibacterial, and antifungal properties. This study investigated the stability of stilbenes under UV and fluorescent light exposure, discovering novel compounds formed during the experiments.
Article
Microbiology
Hanna Huuki, Seppo Ahvenjaervi, Paula Lidauer, Milka Popova, Johanna Vilkki, Aila Vanhatalo, Ilma Tapio
Summary: This study aimed to investigate the effect of providing rumen liquid inoculum from adult cows to pre-weaning calves. The results showed that the inoculum enhanced the maturation of rumen bacterial communities in calves, but its effect on eukaryotic communities was unclear and requires further investigation.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
A. Bougouin, A. Hristov, J. Dijkstra, M. J. Aguerre, S. Ahvenjarvi, C. Arndt, A. Bannink, A. R. Bayat, C. Benchaar, T. Boland, W. E. Brown, L. A. Crompton, F. Dehareng, I. Dufrasne, M. Eugene, E. Froidmont, S. van Gastelen, P. C. Garnsworthy, A. Halmemies-Beauchet-Filleau, S. Herremans, P. Huhtanen, M. Johansen, A. Kidane, M. Kreuzer, B. Kuhla, F. Lessire, P. Lund, E. M. K. Minnee, C. Munoz, M. Niu, P. Noziere, D. Pacheco, E. Prestlokken, C. K. Reynolds, A. Schwarm, J. W. Spek, M. Terranova, A. Vanhatalo, M. A. Wattiaux, M. R. Weisbjerg, D. R. Yanez-Ruiz, Z. Yu, E. Kebreab
Summary: This study aimed to predict nitrogen excretion in feces and urine of lactating dairy cows by collecting data from different continents. The study found that simple models based on dry matter intake or nitrogen intake performed well in predicting fecal nitrogen excretion, while simple models based on milk urea nitrogen and nitrogen intake performed better in predicting urinary nitrogen excretion. In the absence of intake data, complex equations using easily measured variables can be used to predict fecal, urinary, or total manure nitrogen excretion, but these equations have lower performance.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Eric E. Newton, Marjukka Lamminen, Partha Ray, Alexander M. Mackenzie, Christopher K. Reynolds, Michael R. F. Lee, Anni Halmemies-Beauchet-Filleau, Aila Vanhatalo, Sokratis Stergiadis
Summary: This study found that feeding dairy cows different protein supplements did not significantly affect milk and blood plasma mineral concentrations, but could impact fecal mineral concentrations and apparent digestibility. Feeding specific supplements may also decrease iodine concentrations in milk, affecting consumer iodine intake.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Microbiology
Hanna Huuki, Miika Tapio, Paivi Mantysaari, Enyew Negussie, Seppo Ahvenjarvi, Johanna Vilkki, Aila Vanhatalo, Ilma Tapio
Summary: In this study, the post-weaning rumen microbiota development differences were examined in monozygotic twin-heifers that were inoculated or not inoculated with rumen liquid. The results showed that the orally administered microbial inoculant induced transient changes in early rumen microbiome maturation and may influence the later production performance of cows.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Applied
Nanbing Qin, Asta H. Petursdottir, David J. Humphries, Natasa Desnica, Eric E. Newton, Aila Vanhatalo, Anni Halmemies-Beauchet-Filleau, Luke Bell, D. Ian Givens, Darren T. Juniper, Helga Gunnlaugsdottir, Sokratis Stergiadis
Summary: Seaweed supplement can increase milk iodine content, but care should be taken to avoid excessive levels that may pose nutritional risks for young children.
Article
Agriculture, Dairy & Animal Science
S. E. Raisanen, K. Kuoppala, P. Rissanen, A. Halmemies-Beauchet-Filleau, T. Kokkonen, A. Vanhatalo
Summary: The objective of this experiment was to investigate the effect of different forage and concentrate types on lactational performance and nitrogen utilization in lactating dairy cows. The results showed that red clover-grass silage combined with rapeseed expeller resulted in the highest nitrogen efficiency.
JOURNAL OF DAIRY SCIENCE
(2023)
Meeting Abstract
Agriculture, Dairy & Animal Science
S. E. Raisanen, K. Kuoppala, P. Rissanen, A. Halmemies-Beauchet-Filleau, T. Kokkonen, A. Vanhatalo
JOURNAL OF DAIRY SCIENCE
(2022)