期刊
BRITISH JOURNAL OF NUTRITION
卷 103, 期 5, 页码 686-695出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0007114509992285
关键词
Vitamin C; DNA damage; Genomics; Nucleotide excision repair
资金
- Food Standards Agency [T01016]
Intracellular vitamin C acts to protect cells against oxidative stress by intercepting reactive oxygen (ROS) and minimising DNA damage. However, rapid increases in intracellular vitamin C may induce ROS subsequent DNA damage priming DNA repair processes. Herein, we examine the potential of vitamin C and the derivative ascorbate-2-phosphate (2-AP) to induce a nucleotide excision repair (NER) response to DNA damage in a model of peripheral blood mononuclear cells. Exposure of cells to elevated levels of vitamin C induced ROS activity, resulting in increased levels of deoxycytidine glyoxal (gdC) and 8-oxo-2'-deoxygyanosine (8-oxodG) adducts in DNA; a stress response was also induced by 2-AP, but was delayed in comparison to vitamin C. Evidence of gdC repair was also apparent. Measurement of cyclobutane thymine-thymine dimers (T < > T) in DNA and culture supernatant were included as a positive marker for NER activity; this was evidenced by a reduction in DNA and increases in culture supernatant levels of T < > T for vitamin C-treated cells. Genomics analysis fully supported these findings confirming that 2-AP, in particular, induced genes associated with stress response, cell cycle arrest, DNA repair and apoptosis, and additionally provided evidence for the involvement of vitamin C in the mobilisation of intracellular catalytic Fe.
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