期刊
ANALYTICAL CHEMISTRY
卷 87, 期 13, 页码 6794-6800出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.5b01151
关键词
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资金
- EPSRC via the PSIBS doctoral training centre [EP/F50053X/1]
- Astra Zeneca
- National Physical Laboratory
- Birmingham Science City Translational Medicine
- Experimental Medicine Network of Excellence Project
- Advantage West Midlands
- Engineering and Physical Sciences Research Council [1239476, 965135, EP/L023490/1] Funding Source: researchfish
- EPSRC [EP/L023490/1] Funding Source: UKRI
Previously we have shown that liquid extraction surface analysis (LESA) mass spectrometry is suitable for the analysis of intact proteins from a range of biological substrates. Here we show that LESA: mass spectrometry may be coupled with high field asymmetric waveform ion mobility spectrometry (FAIMS) for top-down protein analysis directly from thin tissue sections,(mouse liver, mouse brain) and from bacterial colonies (Escherichia coli) growing on agar. Incorporation of PALMS results in significant improvements in signal-to-noise and reduced analysis time. Abundant protein signals are observed in single scan mass spectra. In addition, FAIMS enables gas-phase separation of molecular classes, for example, lipids and proteins, enabling improved analysis of both sets of species from a single LESA extraction.
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