Article
Biochemistry & Molecular Biology
Mohammad Mousaei Ghasroldasht, Jin Seok, Hang-Soo Park, Farzana Begum Liakath Ali, Ayman Al-Hendy
Summary: Regenerative medicine is a promising therapeutic mode using stem cells for the treatment of challenging human diseases. Stem cells have pleotropic therapeutic potential through inflammation or apoptosis inhibition, cell recruitment, angiogenesis stimulation, and differentiation. This review provides an overview of the translation process from idea to clinical practice, including guidelines for clinical trial approval and a review of regenerative medicine clinical trials registered on the Clinicaltrials.gov website.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Katia Mareschi, Elena Marini, Alessia Giovanna Santa Banche Niclot, Marta Barone, Giuseppe Pinnetta, Aloe Adamini, Manuela Spadea, Luciana Labanca, Graziella Lucania, Ivana Ferrero, Franca Fagioli
Summary: This study demonstrates that the use of HPL-S is an effective alternative for MSC production under GMP conditions. The protein and growth factor content in HPL-S is comparable to that of HPL-E. Furthermore, MSCs derived from HPL-S show lower HLA-DR expression in immunophenotypic analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Michaela Oeller, Sandra Laner-Plamberger, Linda Krisch, Eva Rohde, Dirk Strunk, Katharina Schallmoser
Summary: HPL serves as a valuable alternative to fetal bovine serum in cell culture, allowing for efficient and animal serum-free cell propagation. Providers must adhere to strict quality control and regulatory requirements to ensure safe and GMP-compliant production of HPL. The starting material for HPL preparation is fresh or expired platelet concentrates from healthy donors, and the process may involve pathogen reduction technologies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Saeed Oraee-Yazdani, Roozbeh Tavanaei, Fatemeh Rostami, Atieh Hajarizadeh, Marzieh Mehrabadi, Mohammadhosein Akhlaghpasand, Mona Tamaddon, Samin Khannejad, Kaveh Oraii Yazdani, Alireza Zali
Summary: This study examined the safety of suicide gene therapy using allogeneic adipose tissue-derived mesenchymal stem cells (ADSCs) carrying herpes simplex virus-thymidine kinase (HSV-TK) gene in patients with recurrent glioblastoma multiforme (GBM). The results showed that this gene therapy protocol was safe and well tolerated, and there were improvements in overall survival and progression-free survival. Further clinical trials are needed to validate these findings and investigate the efficacy of this protocol.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Engineering, Biomedical
Yufeng Shou, Ling Liu, Qimin Liu, Zhicheng Le, Khang Leng Lee, Hua Li, Xianlei Li, Dion Zhanyun Koh, Yuwen Wang, Tong Ming Liu, Zheng Yang, Chwee Teck Lim, Christine Cheung, Andy Tay
Summary: Bone marrow-derived mesenchymal stem cells (MSCs) are widely studied due to their regenerative potential and immunomodulatory properties. In this work, a 3D dynamic hydrogel using magneto-stimulation is introduced for direct manufacturing of MSCs. The technology enhances MSC spreading, proliferation, and biofunctions, and can integrate MSC manufacturing to final clinical use, offering a paradigm shift in MSC therapy.
BIOACTIVE MATERIALS
(2023)
Article
Cell & Tissue Engineering
Aekkachai Tuekprakhon, Siripakorn Sangkitporn, Adisak Trinavarat, Aulia Rahmi Pawestri, Visit Vamvanij, Monchai Ruangchainikom, Panya Luksanapruksa, Phitchapa Pongpaksupasin, Areerat Khorchai, Acharaporn Dambua, Patcharaporn Boonchu, Chonlada Yodtup, Mongkol Uiprasertkul, Somchai Sangkitporn, La-ongsri Atchaneeyasakul
Summary: Intravitreal injection of BM-MSCs was found to be safe and potentially effective in treating advanced RP. The study observed mild and transient adverse events in the short-term, with improvements in BCVA but no remarkable progression in VF and CST. Long-term follow-up suggested the need for further assessment with larger sample sizes and less advanced cases.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Katia Mareschi, Alessia Giovanna Santa Banche Niclot, Elena Marini, Elia Bari, Luciana Labanca, Graziella Lucania, Ivana Ferrero, Sara Perteghella, Maria Luisa Torre, Franca Fagioli
Summary: This study modified the type of culture supplement used in the production of freeze-dried mesenchymal stem cell (MSC)-secretome (lyo-secretome), and found that changing the supplement type only resulted in minimal changes in product composition without affecting potency, validating the new process according to GMP standards.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Alison J. Wilson, Emma Rand, Andrew J. Webster, Paul G. Genever
Summary: Many clinical trials involving mesenchymal stem/stromal cells lack comprehensive characterization data of the cellular population, including details on surface marker expression, viability, and differentiation. Functional assessment is very limited and does not always correlate with the likely mechanism of action.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Medicine, Research & Experimental
Marina Gazdic Jankovic, Miodrag Stojkovic, Sanja Bojic, Nemanja Jovicic, Marina Miletic Kovacevic, Zeljko Ivosevic, Aleksandar Juskovic, Vojin Kovacevic, Biljana Ljujic
Summary: Human mesenchymal stem cells (hMSCs) have great potential in regenerative medicine and clinical research. However, the limitation of insufficient hMSCs within adult organs and the high doses required for transplantation necessitate ex vivo expansion. Bioreactor-based cell expansion provides high-level production of hMSCs according to good manufacturing practice (GMP) and quality standards. This review summarizes the current knowledge about hMSCs manufacturing platforms, with a focus on the application of bioreactors for large-scale production of GMP-grade hMSCs.
CURRENT RESEARCH IN TRANSLATIONAL MEDICINE
(2023)
Review
Cell Biology
Danielle M. Wiese, Catherine A. Wood, Lorena R. Braid
Summary: Retrospective analysis of clinical trial outcomes is crucial for efficient translation of cellular therapies, especially for mesenchymal stem/stromal cell (MSC) products. However, current clinical trial reports lack sufficient information on the methodology of cell preparation and handling, leading to significant variability in cell products between trials and hindering the identification of optimal handling protocols.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Clinical Neurology
Jasan A. Berard, Mark S. Freedman, Ruth Ann Marrie, James J. Marriott, Harold L. Atkins, David Szwajcer, David. W. Courtman, Simon Thebault, Lisa A. S. Walker
Summary: This study aimed to evaluate the effects of mesenchymal stem cell therapy on cognition in multiple sclerosis (MS) patients. The results showed that there was no improvement in cognition in the short-term, but there were also no lasting deleterious effects. This supports MSC therapy as a treatment option for MS.
MULTIPLE SCLEROSIS AND RELATED DISORDERS
(2022)
Review
Cell & Tissue Engineering
Ria Margiana, Alexander Markov, Angelina O. Zekiy, Mohammed Ubaid Hamza, Khalid A. Al-Dabbagh, Sura Hasan Al-Zubaidi, Noora M. Hameed, Irshad Ahmad, R. Sivaraman, Hamzah H. Kzar, Moaed E. Al-Gazally, Yasser Fakri Mustafa, Homayoon Siahmansouri
Summary: MSCs have great potential for immunomodulation and tissue regeneration, and can be used for the treatment of various diseases. Recent advances in MSCs biology have facilitated their clinical application.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Biochemistry & Molecular Biology
Batoul Chouaib, Mandana Haack-Sorensen, Franck Chaubron, Frederic Cuisinier, Pierre-Yves Collart-Dutilleul
Summary: Mesenchymal stem cell secretome or conditioned medium (MSC-CM) is a crucial starting point for various derived products and can be used for regenerative medicine. However, issues such as manufacturing protocols and quality control must be addressed before their clinical application. This review highlights the importance of standardized procedures in conditioned medium production and the influence of various factors on the quality of the secretome and its derivatives.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Xiao-Fei Zhang, Ting Wang, Zi-Xuan Wang, Kun-Peng Huang, Yun-Wei Zhang, Guo-Liang Wang, Hong-Ji Zhang, Zi-Han Chen, Chang-Yan Wang, Jin-Xiang Zhang, Hui Wang
Summary: In a hypoxic microenvironment, ucMSCs enhance endothelial cell proliferation and migration through exosome-mediated paracrine function, accelerating wound healing. miR-125b in hypoxic exosomes targets and suppresses TP53INP1, alleviating hypoxia-induced cell apoptosis and promoting cutaneous repair.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Oncology
Arantza Infante, Blanca Gener, Miguel Vazquez, Nerea Olivares, Arantza Arrieta, Gema Grau, Isabel Llano, Luis Madero, Ana Maria Bueno, Belen Sagastizabal, Daniela Gerovska, Marcos J. Arauzo-Bravo, Itziar Astigarraga, Clara I. Rodriguez
Summary: The study demonstrated the safety and efficacy of reiterative MSCs infusion for pediatric OI patients, with improved bone parameters and quality of life during the treatment period and 2-year follow-up. Mechanistic studies indicated that MSCs therapy triggered a pro-osteogenic paracrine response in patients, especially in those with severe OI.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Article
Immunology
K. Jones, L. Wockner, R. M. Brennan, C. Keane, P. K. Chattopadhyay, M. Roederer, D. A. Price, D. K. Cole, B. Hassan, K. Beck, D. Gottlieb, D. S. Ritchie, J. F. Seymour, F. Vari, P. Crooks, S. R. Burrows, M. K. Gandhi
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2016)
Article
Dermatology
Penny Poh Lu Lim, Pablo Fernandez Penas, David Gottlieb
AUSTRALASIAN JOURNAL OF DERMATOLOGY
(2016)
Article
Oncology
Lisa Brice, Nicole Gilroy, Gemma Dyer, Masura Kabir, Matt Greenwood, Stephen Larsen, John Moore, John Kwan, Mark Hertzberg, Louisa Brown, Megan Hogg, Gillian Huang, Jeff Tan, Christopher Ward, David Gottlieb, Ian Kerridge
SUPPORTIVE CARE IN CANCER
(2017)
Article
Cell & Tissue Engineering
Varda S. Sardesai, Abbas Shafiee, Nicholas M. Fisk, Rebecca A. Pelekanos
STEM CELLS TRANSLATIONAL MEDICINE
(2017)
Article
Dentistry, Oral Surgery & Medicine
G. Dyer, L. Brice, M. Schifter, N. Gilroy, M. Kabir, M. Hertzberg, M. Greenwood, S. R. Larsen, J. Moore, D. Gottlieb, G. Huang, M. Hogg, L. Brown, J. Tan, C. Ward, I. Kerridge
AUSTRALIAN DENTAL JOURNAL
(2018)
Article
Hematology
Ian Nivison-Smith, Samuel Milliken, Anthony J. Dodds, David Gottlieb, John Kwan, David D. F. Ma, Peter J. Shaw, Steven Tran, Leonie Wilcox, Jeff Szer
BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION
(2018)
Article
Hematology
David Kliman, Gloria Castellano-Gonzalez, Barbara Withers, Janine Street, Elizabeth Tegg, Oksana Mirochnik, Joey Lai, Leighton Clancy, David Gottlieb, Emily Blyth
BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION
(2018)
Article
Hematology
Chun K. K. Ma, Leighton Clancy, Renee Simms, Jane Burgess, Shivashni Deo, Emily Blyth, Kenneth R. Micklethwaite, David J. Gottlieb
BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION
(2018)
Letter
Biophysics
Gemma Dyer, Lisa Brice, Nicole Gilroy, Masura Kabir, Mark Hertzberg, Matt Greenwood, Stephen R. Larsen, John Moore, David Gottlieb, Gillian Huang, Megan Hogg, Louisa Brown, Jeff Tan, Christopher Ward, Ian Kerridge
BONE MARROW TRANSPLANTATION
(2018)
Article
Biotechnology & Applied Microbiology
David C. Bishop, Ning Xu, Benjamin Tse, Tracey A. O'Brien, David J. Gottlieb, Alla Dolnikov, Kenneth P. Micklethwaite
Article
Medicine, General & Internal
David Kliman, Ian Bilmon, John Kwan, Emily Blyth, Ken Micklethwaite, Shyam Panicker, Abir Bhattacharyya, Stephanie Deren, Vicki Antonenas, Gillian Huang, David Gottlieb
INTERNAL MEDICINE JOURNAL
(2018)
Article
Immunology
Gaurav Sutrave, Adam Maundrell, Caitlin Keighley, Zoe Jennings, Susan Brammah, Min-Xia Wang, Roger Pamphlett, Cameron E. Webb, Damien Stark, Helen Englert, David Gottlieb, Ian Bilmon, Matthew R. Watts
EMERGING INFECTIOUS DISEASES
(2018)
Article
Cell & Tissue Engineering
Chun K. K. Ma, Leighton Clancy, Shivashni Deo, Emily Blyth, Kenneth P. Micklethwaite, David J. Gottlieb
Review
Cell & Tissue Engineering
Gaurav Sutrave, Emily Blyth, David J. Gottlieb
Review
Infectious Diseases
Gloria Castellano-Gonzalez, Leighton E. Clancy, David Gottlieb
CURRENT OPINION IN INFECTIOUS DISEASES
(2017)
