Article
Agronomy
Dandan Cui, Shanshan Zhai, Yao Yang, Yuhua Wu, Jun Li, Xiaohong Yan, Ping Shen, Hongfei Gao, Gang Wu
Summary: An unmarked electrochemical impedance genosensor using gold carbon dots has been developed for the detection of genetically modified crops. This sensor is portable and easy to use, making it suitable for resource-limited laboratories.
Article
Chemistry, Multidisciplinary
Yujun Xing, Jie Liang, Fei Dong, Jirong Wu, Jianrong Shi, Jianhong Xu, Jinke Wang
Summary: We developed a novel loop-mediated isothermal amplification (LAMP) method using DNA captured on polyacrylamide microparticles (PAMMPs) as templates (PAMMPs@DNA-LAMP) for rapid qualitative detection of genetically modified organisms (GMOs). The PAMMPs@DNA-LAMP method allowed for rapid DNA extraction (5-10 min) and amplification (around 30 min) at a constant temperature of 63°C. The DNA captured by PAMMPs could be effectively detected by both conventional and quantitative PCR (qPCR) and LAMP.
Article
Chemistry, Analytical
Ece Eksin
Summary: A new genosensor was developed for sensitive and selective detection of the gene sequence of Acinetobacter baumannii. It utilizes a capture probe immobilized on chitosan modified single-use pencil graphite electrodes (c-PGEs) to recognize the target DNA sequence. The genosensor showed a low limit of detection and no interference from non-complementary sequences.
Article
Chemistry, Analytical
Jing-bo Jiao, Qing Kang, Ting Du, Yu-fei Fan, Ya-ping Huang, Dong-dong Zhu, Chu-min Zhao, Xiao-yi Wang, Xin-jun Du, Shuo Wang
Summary: In this study, a genosensor platform based on DNA biofunctionalized superparamagnetic nickel colloidal nanocrystal clusters (SNCNCs) coupled with IS-primer amplification reaction (ISAR) was developed for rapid, ultrasensitive, and specific detection of Listeria monocytogenes (L. monocytogenes). The method showed high sensitivity, good specificity, convenience, and low cost, making it a promising tool for bacterial detection in food, environment, and clinics.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Yanping Zhang, Ruomeng Li, Shanshan Yu, Jinhua Shang, Yuqiu He, Yushi Wang, Xiaoqing Liu, Fuan Wang
Summary: In this study, a simple autocatalytic hybridization circuit (AHC) is proposed for high-reliability in situ intracellular imaging of polynucleotide kinase (PNK). The AHC system amplifies the signal through two mutually activated hybridization chain reaction (HCR) modules, allowing for reliable detection and imaging of PNK.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Hessamaddin Sohrabi, Mir Reza Majidi, Fatemeh Nami, Karim Asadpour-Zeynali, Alireza Khataee, Ahad Mokhtarzadeh
Summary: The novel label-free DNA genosensing assay developed in this study, based on Zn-based MOF and AuNPs surface modification, demonstrates high specificity and sensitivity for recognizing the H. influenza genome in human plasma samples. Various characterization techniques validated its performance, with LOD and LOQ under optimal conditions being 1.48 fM and 3.23 fM respectively, suitable for the determination of the L-fuculokinase gene.
Article
Chemistry, Analytical
Yuting Yan, Fangfang Zhou, Qian Wang, Yue Huang
Summary: A sensitive electrochemical biosensor is developed using Cu2+-modulated signal amplification for the detection of quinolones. The presence of target quinolones inhibits the activity of Cu2+-specific DNAzyme, resulting in the amplification of electrochemical signal positively correlated with the concentration of quinolones. The biosensor exhibits a good linear relationship and low limit of detection, making it suitable for the detection of quinolones in foodstuff samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Biophysics
Meijuan Liang, Xinfa Cai, Yaoyao Gao, Honglin Yan, Jiayun Fu, Xiaoqian Tang, Qi Zhang, Peiwu Li
Summary: In this study, a compact Cu-anchored PDA (PCu) was engineered as the efficient signal element for multimodal LFA (mLFA), providing triple signal readouts and highly reliable analytical performance.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Applied
Luis Antonio Tortajada-Genaro, Angel Maquieira
Summary: The development of a method combining RPA and hybridization analysis allows for rapid and accurate detection of transgenic ingredients in food, supporting compliance with product labeling and traceability regulations. The optical sensing of RPA products using compact disc technology showed excellent selectivity, reproducibility, and sensitivity. This isothermal method provides an effective high-throughput tool for simple and low-cost food safety controls, ideal for laboratories with limited resources.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Chemistry, Analytical
Xiuxiu Zhang, Fusheng Liao, Mei Wang, Jie Zhang, Binxiang Xu, Ling Zhang, Jun Xiong, Wei Xiong
Summary: A sensitive electrochemical thrombin aptasensor was proposed in this work, utilizing CHA and bisferrocene to detect thrombin concentration. The sensor demonstrated a wide linear range and high detection limit, making it suitable for monitoring thrombin in human serum samples.
Article
Chemistry, Analytical
Jingyi Cai, Junping Ma, Yuan Dang, Shuangli Chen, Jieli Tang, Sha Yu, Yuanzhen Zhou
Summary: Antibiotics residue pollution in food and environmental matrices is a serious threat to global public health. It is urgent to develop highly sensitive methods for detecting low-abundance antibiotics in real samples. In this study, a magnetic DNA walker-engineered electrochemical sensor was established, using the specific recognition capability of aptamer and the signal amplification performance of magnetic DNA walker, for accurate detection of kanamycin (Kana). The sensor showed good linear relationship in the range of 10 fM - 1 μM, with a low limit of detection of 0.02 fM. It also demonstrated excellent selectivity, being able to specifically identify Kana from other antibiotics and even in milk and tap water.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Lizhen Liu, Meihong Peng, Zerong Liang, Hong Wu, Hailong Yan, Yi-Ge Zhou
Summary: Nanoparticle impact electrochemistry (NIE) is a promising technique for sensitive detection of various biological species. Researchers have developed an NIE-based sensor using T-Hg2+-T coordination induced AgNP aggregation to detect Hg2+ in water. The proposed aggregation-collision strategy enables highly sensitive and selective detection, and a dual-mode analysis enhances accuracy in quantification. The NIE-based sensor shows reliable analysis of Hg2+ in real water samples, making it a potential tool for environmental monitoring and point-of-care testing applications.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Hongkun Li, Qianqian Cai, Xiaoshi Yan, Guifen Jie, Guitao Jie
Summary: A novel ratiometric electrochemical biosensor was developed for sensitive detection of microRNA using walker amplification technique. The biosensor showed excellent sensitivity and high specificity, with a wide linear detection range of target concentration. The internal reference signal of MB and ratio of Ag NPs to MB greatly reduced background noise, making it a promising tool for bioanalysis and disease diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Hongli Zhao, Zhenmin Niu, Kaicha Chen, Lijuan Chen, Zhenxing Wang, Minbo Lan, Jinxiu Shi, Wei Huang
Summary: A novel sandwich-type electrochemical biosensor was developed for sensitive detection of ctDNA using nanocomposites as signal probes' label. The sensor showed excellent detection performance with wide linear range and low detection limit, demonstrating promising practical applications in clinical ctDNA diagnosis.
MICROCHEMICAL JOURNAL
(2021)
Review
Biochemical Research Methods
Ramon Lorenzo-Gomez, Rebeca Miranda-Castro, Noemi De-los-Santos-alvarez, Maria Jesus Lobo-Castanon
Summary: The role of extracellular matrix (ECM) in tumorigenesis and metastasis is crucial, as tumor cells need to recruit a permissive microenvironment for growth. The interaction between tumor cells and ECM leads to the release of ECM-derived molecules into the bloodstream, which can serve as potential biomarkers for tumor development. Developing better bioanalytical methods for detecting ECM remodeling-derived components is essential for translating basic knowledge about cancer progression into clinical practice.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Ramon Lorenzo-Gomez, Rebeca Miranda-Castro, Juan R. de los Toyos, Noemi de-los-Santos-Alvarez, Maria Jesus Lobo-Castanon
Summary: The ECM plays a crucial role in tumor progression and invasion. The overexpression of the alpha 1 chain of XI collagen in some tumor stroma may serve as a potential cancer biomarker.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Ramon Lorenzo-Gomez, Alfonso Casero-Alvarez, Rebeca Miranda-Castro, Marcos Garcia-Ocana, Juan R. de los Toyos, Noemi de-los-Santos-Alvarez, M. Jesus Lobo-Castanon
Summary: Characterization of extracellular matrix plays a crucial role in understanding cancer progression. This study presents an electrochemical aptamerbased competitive assay for detecting dysregulated collagen XI in various carcinomas. The assay demonstrates high sensitivity and accuracy, allowing detection of the target peptide in cell lysates and serum.
Article
Chemistry, Analytical
Raquel Sanchez-Salcedo, Rebeca Miranda-Castro, Noemi de-los-Santos-Alvarez, Daniel Fernandez-Martinez, Luis Joaquin Garcia-Florez, Maria Jesus Lobo-Castanon
Summary: This study developed an electrochemical platform for the relative quantification of a lncRNA called CCAT1, which is associated with colorectal cancer. The platform showed high sensitivity and low limits of detection, and preliminary results suggest its potential for detecting other lncRNAs associated with malignancies, facilitating early cancer diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemical Research Methods
Cristina Munoz-San Martin, Ana Montero-Calle, Maria Garranzo-Asensio, Maria Gamella, Victor Perez-Gines, Maria Pedrero, Jose M. Pingarron, Rodrigo Barderas, Noemi de-los-Santos-Alvarez, Maria Jesus Lobo-Castanon, Susana Campuzano
Summary: This work presents the first bioelectronic immunoplatforms for determining glycosylated haptoglobin (Hp) and both total and glycosylated Hp simultaneously. The immunoplatforms use non-competitive bioassays and magnetic microcarriers labeled with horseradish peroxidase (HRP) for detection. The method allows accurate determination of total and glycosylated Hp in colorectal cancer (CRC) cells, which is not possible with commercial ELISA kits and Western blot methodology due to lack of sensitivity.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Physical
Noemi de-los-Santos-Alvarez, Maria Jesus Lobo-Castanon, Miguel Aller Pellitero
Summary: The development of simple, rapid, and cost-effective electrochemical strategies for cancer biomarker detection is crucial for early cancer diagnosis and treatment monitoring. Aptamer-based electrochemical assays, such as nanopore-sensing, label-free, and signal amplification systems, have shown great potential in overcoming challenges in cancer detection and integrating biomolecular data with health systems.
CURRENT OPINION IN ELECTROCHEMISTRY
(2023)
Review
Chemistry, Physical
Madalin Alexandru Cobzariu, Maria Jesus Lobo-Castanon, Rebeca Miranda-Castro
Summary: The detection of genetic material is crucial in clinical practice, food quality control, and environmental analysis. Nucleic acid tests involve amplification to detect the low amount of target sequences, with enzyme-assisted isothermal amplification strategies such as HDA, LAMP, and RPA being easier to adapt to electrochemical platforms for point-of-need applications compared to PCR. This review discusses the current state and challenges of coupling these amplification strategies to conductive platforms for electrochemical transduction in real-world point-of-need tests.
CURRENT OPINION IN ELECTROCHEMISTRY
(2023)
Article
Biochemical Research Methods
Raquel Sanchez-Salcedo, Rebeca Miranda-Castro, Noemi de-los-Santos-Alvarez, Maria Jesus Lobo-Castanon, Damion K. Corrigan
Summary: A capacitive electrochemical impedance spectroscopy sensing platform tailored for point-of-care detection and treatment monitoring has been developed. The specific recognition of IL-6 was achieved using an anti-IL-6 nanobody or a specific IL-6 aptamer. The results provide useful information for the future design of low-cost and wearable impedance biomarker detection platforms.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Ana Diaz-Fernandez, Noemi de-los-Santos-Alvarez, Maria Jesus Lobo-Castanon
Summary: Wearable sensors have the potential to revolutionize healthcare and personalized medicine, providing continuous and real-time data for individuals. Capacitive sensors technology can enrich wearable devices by enabling more accurate physiological indicators. However, there are challenges that need to be overcome to fully utilize capacitive transduction in wearable sensors.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)