期刊
BRITISH JOURNAL OF CANCER
卷 111, 期 9, 页码 1765-1771出版社
SPRINGERNATURE
DOI: 10.1038/bjc.2014.484
关键词
microRNA; stool biomarker; colorectal cancer; diagnosis
类别
资金
- Shenzhen Technology and Innovation Project Fund, Shenzhen [JSGG20130412171021059]
- China 863 programme [2012AA02A506]
- China 973 Programme [2013CB531401]
- ITF fund Hong Kong [ITS/276/11]
- Shenzhen Municipal Science and Technology RD fund [JCYJ JCYJ20120619152326450]
- Shenzhen Virtual University Park Support Scheme
Background: The detection of microRNA (miRNA) dysregulation in stool is a novel approach for the diagnosis of colorectal carcinoma (CRC). The aim of this study is to investigate the use of miR-221 and miR-18a in stool samples as non-invasive biomarkers for CRC diagnosis. Methods: A miRNA expression array containing 667 miRNAs was performed to identify miRNA dysregulation in CRC tissues. We focused on miR-221 and miR-18a, two significantly upregulated miRNAs which were subsequently verified in 40 pairs of CRC tissues and 595 stool samples (198 CRCs,199 polyps and 198 normal controls). Results: miR-221 and miR-18a were upregulated in the miRNA expression array. miR-221 and miR-18a levels were also significantly higher in 40 CRC tumours compared with their respective adjacent normal tissues. In stool samples, miR-221 and miR-18a showed a significant increasing trend from normal controls to late stages of CRC (Po0.0001). The levels of stool miR-221 and miR-18a were both significantly higher in subjects with stages I + II (miR-221: P<0.0001, miR-18a: P<0.0001) and stages III + IV of CRC (miR-221: P = 0.0004, miR-18a: P<0.0001) compared with normal controls. The AUC of stool miR-221 and miR-18a were 0.73 and 0.67 for CRC patients as compared with normal controls, respectively. No significant differences in stool miR-221 and miR-18a levels were found between patients with proximal and distal CRCs. The use of antibiotics did not influence stool miRNA-221 and miRNA-18a levels. Conclusions: Stool-based miR-221 can be used as a non-invasive biomarker for the detection of CRC.
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