Article
Oncology
Bo Zhan, Xiao Dong, Yulin Yuan, Zheng Gong, Bohan Li
Summary: The study reveals that HIF-1α is downregulated in ccRCC, inhibiting cell proliferation, migration, and invasion, which has important biological implications.
FRONTIERS IN ONCOLOGY
(2021)
Review
Oncology
Sonia Mazumder, Paul J. Higgins, Rohan Samarakoon
Summary: Mutations in the VHL gene contribute to the development of renal cell carcinoma, particularly the clear cell variant. HIF-2 alpha plays a crucial role in promoting tumor progression and metastasis in these cases. Inhibiting HIF-2 alpha and its associated molecular pathways can be an effective strategy to suppress the aggressiveness of ccRCC.
Article
Pharmacology & Pharmacy
Yangyang Zhan, Yang Liu, Rui Yang, Qiong Chen, Fei Teng, Yueying Huang, Xin Jiang, Yueming Wang, Bin Yu, Ding Zhang, Leilei Bao, Xinli Liu, Jiwei Huang
Summary: The circular RNA circPTEN was found to suppress ccRCC progression and resistance to mTOR inhibitors by inhibiting the expression of its parental gene PTEN and GLUT1 through methylation regulation.
DRUG RESISTANCE UPDATES
(2023)
Article
Oncology
Fengqi Yan, Qinhao Wang, Mingyuan Xia, Yi Ru, Wei Hu, Guang Yan, Xin Xiong, Mei Zhang, Jiancai Wang, Qi Li, Bo Zhang, He Wang, Wei Lin, Guojun Wu, Xia Li
Summary: MIIP is identified as a novel tumor suppressor in ccRCC, inhibiting cancer cell proliferation and angiogenesis through negative regulation of the HIF-2 alpha-CYR61 axis.
CANCER BIOLOGY & MEDICINE
(2022)
Article
Oncology
Kun Jiang, Li-zhe Xu, Jin-zhuo Ning, Fan Cheng
Summary: This study aimed to investigate the expression of FAP and its clinical implication in ccRCC. Computational tools analysis of gene databases revealed that FAP levels were significantly elevated in ccRCC. Higher expression levels of FAP were associated with poorer prognoses in ccRCC patients, and FAP may serve as a reliable biomarker for the diagnosis and prognosis of ccRCC.
CANCER CELL INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Jing Zhou, Ning Zhang, Wei Zhang, Caiju Lu, Fei Xu
Summary: The study found that YAP, HIF-1α, and miR-182 were upregulated in peripheral blood mononuclear cells from patients with asthma, while EGR2 was downregulated. The upregulation of YAP and HIF-1α promoted the expression of miR-182, which in turn inhibited EGR2 and enhanced Th17 cell differentiation, worsening asthma and lipid metabolism dysfunction.
CELL AND BIOSCIENCE
(2021)
Article
Oncology
Min Yin, Chunli Lu, Huimei Zhou, Qian Liu, Jiaxin Yang
Summary: While the pathogenesis of ovarian clear cell carcinoma (OCCC) has been widely studied, the mechanism of chemoresistance remains unknown. This study identified fibroblast growth factor 11 (FGF11) as a key gene associated with platinum resistance in OCCC tissues. Inhibition of FGF11 reversed the resistance to cisplatin and suppressed the progression of OCCC. Mechanistically, FGF11 expression was regulated by hypoxia-inducible factor-1α (HIF-1α) to modulate cisplatin sensitivity.
CANCER MANAGEMENT AND RESEARCH
(2023)
Article
Cell Biology
Jing Ji, Yuxin Xu, Mengru Xie, Xingbei He, Dexu Ren, Teng Qiu, Wenwen Liu, Zefeng Chen, Wen Shi, Zhen Zhang, Xiujun Wang, Weiling Wang, Jinming Ma, Qilan Qian, Aixin Jing, Xinhui Ma, Jingting Qin, Yuanyuan Ding, Ting Geng, Jiayan Yang, Zhichao Sun, Wei Liu, Shunfang Liu, Bin Liu
Summary: This study revealed that SEMA6A is overexpressed in clear cell renal cell carcinoma (ccRCC) and promotes ccRCC progression through the activation of the Wnt/β-catenin pathway and interaction with SEC62. SEMA6A is a potential therapeutic target for VHL-deficient ccRCC.
CELL DEATH & DISEASE
(2023)
Article
Biology
Maeva Dufies, Annelies Verbiest, Lindsay S. Cooley, Papa Diogop Ndiaye, Xingkang He, Nicolas Nottet, Wilfried Souleyreau, Anais Hagege, Stephanie Torrino, Julien Parola, Sandy Giuliano, Delphine Borchiellini, Renaud Schiappa, Baharia Mograbi, Jessica Zucman-Rossi, Karim Bensalah, Alain Ravaud, Patrick Auberger, Andreas Bikfalvi, Emmanuel Chamorey, Nathalie Rioux-Leclercq, Nathalie M. Mazure, Benoit Beuselinck, Yihai Cao, Jean Christophe Bernhard, Damien Ambrosetti, Gilles Pages
Summary: Dufies et al. found high Plk1 expression levels in aggressive clear cell renal cell carcinoma and discovered that Plk1 is transcriptionally upregulated in a manner dependent on HIF-2. They also found that high Plk1 expression is correlated to a poor prognosis and resistance to tyrosine kinase inhibitor against VEGF receptor, suggesting a critical role for hypoxia/HIF-2-induced Plk1 in disease progression.
COMMUNICATIONS BIOLOGY
(2021)
Article
Oncology
Peng Xu, Kang Hu, Ping Zhang, Zhi-Gang Sun, Nan Zhang
Summary: The study reveals that hypoxia-mediated YTHDF2 overexpression promotes cell proliferation and invasion in lung squamous cell carcinoma through activation of the mTOR/AKT signaling pathway, and YTHDF2 is closely associated with pN and pTNM stage, leading to poor prognosis for LUSC patients.
CANCER CELL INTERNATIONAL
(2022)
Article
Genetics & Heredity
Jinxiang Wang, Jie Jin, Yanling Liang, Yihe Zhang, Nisha Wu, Mingming Fan, Fangyin Zeng, Fan Deng
Summary: PRKCE plays a key role in kidney renal clear cell carcinoma (KIRC), and its aberrant expression is associated with clinicopathological variables and prognosis. PRKCE may affect prognosis in KIRC by regulating immune infiltration, and it could serve as a novel therapeutic target.
FRONTIERS IN GENETICS
(2022)
Article
Multidisciplinary Sciences
Zhou Yang, Xiyi Wei, Chengjian Ji, Xiaohan Ren, Wei Su, Yichun Wang, Jingwan Zhou, Zheng Zhao, Pengcheng Zhou, Kejie Zhao, Bing Yao, Ninghong Song, Chao Qin
Summary: OGT is upregulated in ccRCC and its upregulation is associated with worse survival. OGT promotes the proliferation and invasion of VHL-mutated ccRCC cells by inhibiting the degradation of HIF-2α and confers high sensitivity to ferroptosis.
Review
Pathology
Raviprakash Tumkur Sitaram, Marene Landstrom, Goran Roos, Borje Ljungberg
Summary: Renal cell carcinoma consists of various tumor types with genetic abnormalities playing a crucial role in signaling networks, tumorigenesis, and prognosis. Clear cell RCC, the most prevalent type, often has a poor prognosis and is refractory to traditional treatments. In recent years, multiple targeting agents have been developed for ccRCC, but limitations persist in terms of drug resistance and alternate signaling pathways.
JOURNAL OF CLINICAL PATHOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Silviu Constantin Badoiu, Maria Greabu, Daniela Miricescu, Iulia-Ioana Stanescu-Spinu, Radu Ilinca, Daniela Gabriela Balan, Andra-Elena Balcangiu-Stroescu, Doina-Andrada Mihai, Ileana Adela Vacaroiu, Constantin Stefani, Viorel Jinga
Summary: Renal cell carcinoma (RCC) is the most frequent type of renal cancer, accounting for 85-95% of cases. The dysregulation of the PI3K/AKT/mTOR signaling pathway is commonly observed in renal cancer and plays a significant role in tumor growth and survival. Metabolic reprogramming is an important characteristic of cancer cells, allowing them to adapt to nutrient and oxygen-deprived environments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Chi Shang, Xuehai Ou, Hongxing Zhang, Dengke Wei, Qiang Wang, Gang Li
Summary: This study found that the aberrantly high expression of Progranulin (PGRN) in osteosarcoma (OS) was associated with poor prognosis. Inhibiting PGRN expression could repress the proliferation and migration of OS cells, and activating the MAPK signaling pathway reversed these effects. In addition, mesenchymal stem cells (MSCs) regulated PGRN to mediate the malignant behavior of OS cells. Hypoxia enhanced HIF-1α expression in MSCs, and silencing HIF-1α suppressed the oncogenic effects of MSCs and reduced PGRN expression in OS cells.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Panagiotis G. Adamopoulos, Konstantina Athanasopoulou, Panagiotis Tsiakanikas, Andreas Scorilas
Summary: CDK4, an important protein kinase, is implicated in human cancers. Utilizing a novel nanopore sequencing approach, researchers identified previously unknown CDK4 transcripts with potential implications for new diagnostic or therapeutic targets.
Article
Pathology
Rola M. Saleeb, Fadi Brimo, Yuan Gao, Carl Boulos, Sung Sun Kim, Samir Al Bashir, Arjumand Husain, Fabio Rotondo, Vidya Beharry, Georg A. Bjarnason, Adriana Krizova, Kiril Trpkov, George M. Yousef
Summary: The value of the biological grouping in a cohort of 176 PRCC patients was assessed and validated, and tissue microarrays were used to stratify the patients into four groups. The results showed that the biological stratification was significant in predicting overall survival and disease-free survival in PRCC patients.
Article
Medicine, General & Internal
Harrison Sicheng Lin, Qiang Ding, Zsuzsanna Lichner, Sung Sun Kim, Rola Saleeb, Mina Farag, Ashley Di Meo, Pamela Plant, Mirit Kaldas, Georg Arnold Bjarnason, George Makram Yousef
Summary: Resistance to sunitinib treatment is a common issue in metastatic renal cell carcinoma, and individualizing treatment protocols could be the key to solving this problem. Through experiments, it was discovered that alternative treatment schedules could delay cancer progression and result in distinct morphological changes in the tumor compared to traditional treatment schedules.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Cell Biology
Maria-Alexandra Papadimitriou, Konstantina Panoutsopoulou, Katerina-Marina Pilala, Andreas Scorilas, Margaritis Avgeris
Summary: Methylation of DNA/RNA and proteins is crucial for cellular homeostasis and development, and disrupted methylation imprinting is strongly associated with human pathologies, especially cancer. Recent evidence has shown that miRNAs play a significant role in regulating cellular methylation profiles during cancer onset and progression. Epi-miRNAs are a novel subclass of miRNAs that have potential clinical utility in cancer diagnostics and personalized therapeutics.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2023)
Article
Medical Laboratory Technology
Maria-Alexandra Papadimitriou, Panagiotis Levis, Georgios Kotronopoulos, Konstantinos Stravodimos, Margaritis Avgeris, Andreas Scorilas
Summary: This study evaluated the clinical utility of preoperative serum cell-free DNA (cfDNA) in risk stratification and prognosis of bladder cancer patients. The results showed that elevated preoperative cfDNA levels were associated with disease progression and metastasis, and were strongly correlated with short-term metastasis and poor prognosis of muscle-invasive bladder cancer (MIBC) patients.
CLINICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Despoina D. Gianniou, Aimilia D. Sklirou, Maria-Alexandra Papadimitriou, Katerina-Marina Pilala, Konstantinos Stravodimos, Margaritis Avgeris, Andreas Scorilas, Ioannis P. Trougakos
Summary: This study investigated the expression and role of HSPB2 and HSPB3 genes in bladder cancer patients and bladder cancer cells. The results showed increased expression levels of HSPB2 and HSPB3 genes and proteins in high-grade bladder cancer cells. In clinical samples, the expression levels of HSPB2 and HSPB3 genes were significantly downregulated in bladder tumors but showed an upregulation trend in muscle-invasive and high-grade tumors. These findings suggest that HSPB2 and HSPB3 have a pro-survival role in advanced bladder cancer and can be targeted as novel molecular markers for treatment optimization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Genetics & Heredity
Michaela A. A. Boti, Konstantina Athanasopoulou, Panagiotis G. G. Adamopoulos, Diamantis C. C. Sideris, Andreas Scorilas
Summary: In October 2020, Emmanuelle Charpentier and Jennifer A. Doudna were awarded the chemistry Nobel Prize for their discovery of CRISPR-Cas9, a promising genome-editing tool. The identification of CRISPR arrays and cas genes has led to the development of strategies for precise DNA editing, which have been widely used in biological and medical research.
Article
Biochemistry & Molecular Biology
Maria Papasavva, Styliana Amvrosiou, Katerina-Marina Pilala, Konstantinos Soureas, Panayiota Christodoulou, Yuan Ji, Konstantinos Stravodimos, Damo Xu, Andreas Scorilas, Margaritis Avgeris, Maria-Ioanna Christodoulou
Summary: Cellular and molecular immune components, including interleukin-37 (IL-37) and its receptor SIGIRR, play a crucial role in the development and progression of bladder urothelial carcinoma (BLCA). IL-37 levels are associated with tumor development and longer overall survival in BLCA patients. Mutations in SIGIRR are related to increased infiltration of regulatory T cells and dendritic cells in the tumor. These findings suggest that IL-37 and SIGIRR could be potential prognostic and diagnostic markers for BLCA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Konstantina Athanasopoulou, Panagiotis G. G. Adamopoulos, Andreas Scorilas
Summary: Over 10(14) symbiotic microorganisms in a healthy human body are responsible for important synthesis and support of the immune system. However, microbial deregulation can lead to various diseases. The human gastrointestinal tract is a favorable environment for diverse microbes. The gut microbiome, the largest microbiome community in the human body, has been extensively studied for its medical significance. Advances in sequencing techniques have enabled the detection of microbiomes and opened new avenues in studying human microbiota. The present review emphasizes the complexity of the gastrointestinal tract microbiome and its implications in cellular homeostasis and human diseases.
Article
Genetics & Heredity
Paraskevi Karousi, Christos K. Kontos, Panagiota Papakotsi, Ioannis K. Kostakis, Alexios-Leandros Skaltsounis, Andreas Scorilas
Summary: This study found that oleuropein and oleocanthal can inhibit the proliferation of triple-negative breast cancer (TNBC) cells and significantly alter the expression profile of TNBC cells. These compounds may have therapeutic benefits for TNBC through their effects on multiple pathways associated with TNBC.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Article
Biochemistry & Molecular Biology
Christos K. Kontos, Paraskevi Karousi, Pinelopi I. Artemaki, Ahmed Abdelgawad, Aspasia Dimitriadou, Nikolaos P. Machairas, Diamantis C. Sideris, Vasiliki Pappa, Andreas Scorilas, Mona Batish, Sotirios G. Papageorgiou
Summary: In this study, we identified several novel circRNAs produced by BAX and BCL2L12, characterized by diverse exon structures. Interestingly, visualization of the most abundant circRNAs showed distinct intracellular localization. Additionally, a complex expression pattern of BAX and BCL2L12 circRNAs was revealed in CLL patients and non-leukemic blood donors, suggesting a multifaceted role in B-cell CLL.
Article
Biochemistry & Molecular Biology
Christos K. Kontos, Despina Hadjichambi, Maria Papatsirou, Paraskevi Karousi, Spyridon Christodoulou, Diamantis C. Sideris, Andreas Scorilas
Summary: This study identifies 23 novel BOK circRNAs, which consist of both exonic and intronic regions of the BOK gene. These circRNAs could play a role in the molecular landscape and clinical investigation of ovarian and prostate cancer.
Review
Biochemistry & Molecular Biology
Panagiotis G. Adamopoulos, Konstantina Athanasopoulou, Glykeria N. Daneva, Andreas Scorilas
Summary: Recent advances in molecular biology have allowed for the identification of post-transcriptional marks on RNAs, leading to the emerging field of epitranscriptomics. Over 170 RNA modifications have been reported, playing crucial roles in RNA biogenesis, stability, and accuracy of transcription and translation. Dysfunctions in the enzymes responsible for RNA modifications can lead to human diseases and cancer. This review focuses on the landscape of epitranscriptomics in human RNAs, describing the most common modifications and their functional and regulatory roles, as well as their involvement in cellular homeostasis and disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Katerina Katsaraki, Christos K. Kontos, Gerasimos Ardavanis-Loukeris, Alexandros A. Tzovaras, Diamantis C. Sideris, Andreas Scorilas
Summary: This study investigates the regulatory effect of proteasome inhibitors (PIs) on miRNA expression in breast cancer (BrCa) cell lines. The results show a high heterogeneity in miRNA levels among different cell lines and different treatment durations. Three miRNAs (miR-1-3p, miR-421-3p, and miR-765-3p) are identified as potential key players in BrCa. Various biological processes, molecular functions, and pathways are predicted to be affected.
CLINICAL & TRANSLATIONAL ONCOLOGY
(2023)
Article
Genetics & Heredity
Paraskevi Karousi, Martina Samiotaki, Manousos Makridakis, Jerome Zoidakis, Diamantis C. Sideris, Andreas Scorilas, Thomas Carell, Christos K. Kontos
Summary: This study investigates the role of 3'-tRF-Cys(GCA) in gene expression regulation in HEK-293 cells and finds that it can regulate gene expression by affecting multiple cellular pathways. Additionally, the study identifies the direct interaction between 3'-tRF-Cys(GCA) and thymopoietin and its modulation of the protein's levels.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
