Article
Biotechnology & Applied Microbiology
Haein Lee, Geunhwa Park, Seulha Kim, Boram Son, Jinmyoung Joo, Hee Ho Park, Tai Hyun Park
Summary: Arginine deiminase (ADI) is a microbial-derived enzyme with anti-tumor activity. However, its clinical application is limited. To overcome these limitations, researchers fused ADI with 30Kc19 alpha, which improved solubility, stability, and cell penetration, demonstrating selective cytotoxicity and reduced drug resistance in melanoma cells.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Medicine, General & Internal
Jin-Shou Yang, Cheng-Cheng Wang, Jiang-Dong Qiu, Bo Ren, Lei You
Summary: PDAC is an extremely malignant disease with a low survival rate, with arginine metabolism playing a crucial role in its development and progression. Arginine deprivation is considered a potential therapy, but drug resistance may develop during treatment. Arginase 1 may be a potential target for cancer immunotherapy.
CHINESE MEDICAL JOURNAL
(2021)
Article
Chemistry, Analytical
N. Ye Stasyuk, G. Z. Gayda, A. E. Zakalskiy, L. R. Fayura, O. M. Zakalska, M. Nisnevitch, M. Gonchar
Summary: Limited data on amperometric biosensors based on deiminases that produce ammonium as a byproduct of enzymatic reaction. Novel biosensors for direct measurement of creatinine and L-arginine were successfully developed, with high sensitivities, broad linear ranges, low limits of detection, and good selectivities towards natural substrates. Real samples of biological fluids and juices were tested, showing high correlations with reference methods for the target analytes.
Article
Endocrinology & Metabolism
Mahmoud A. Mohammad, Inka C. Didelija, Barbara Stoll, Trung C. Nguyen, Juan C. Marini
Summary: ADI-PEG20 administration rapidly depletes circulating arginine while maintaining tissue concentrations, without affecting blood pressure or protein metabolism, demonstrating high citrulline turnover and regeneration of arginine through the citrulline recycling pathway.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2021)
Article
Chemistry, Analytical
Kseniia Berketa, Olga Saiapina, Lyubov Fayura, Andriy Sibirny, Sergei Dzyadevych, Oleksandr Soldatkin
Summary: A novel arginine-sensitive conductometric biosensor based on a recombinant arginine deiminase (ADI) produced in E. coli was developed for the detection of L-arginine. The biosensor exhibited high selectivity and stability, making it suitable for quantification of arginine in dietary supplements.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Oncology
Yu Zhang, Sai-Fung Chung, Suet-Ying Tam, Yun-Chung Leung, Xiao Guan
Summary: Extensive studies have shown the importance of amino acid deprivation in cancer therapy, with arginine playing a pivotal role as a key nutrient for cancer cells. Arginine deiminase and human arginase are major protein drugs used for arginine deprivation, but they face common issues in clinical applications.
Review
Chemistry, Physical
Galina Gayda, Nataliya Stasyuk, Andriy Zakalskiy, Mykhailo Gonchar, Evgeny Katz
Summary: L-Arginine is a semi-essential amino acid that plays important roles in human health. Its metabolism generates various biochemical products and it is used in clinical diagnostics, disease prevention/treatment, and quality assessment of food and beverages. Electrochemical biosensors based on Arg-degrading enzymes are considered promising tools for Arg assay.
CURRENT OPINION IN ELECTROCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Tjeerd Pols, Shubham Singh, Cecile Deelman-Driessen, Bauke F. Gaastra, Bert Poolman
Summary: The breakdown of arginine in cells generates metabolic energy in the form of ATP through a pathway consisting of specific enzymes and a transporter. Various factors such as physicochemical conditions, effector composition, and concentration can affect enzyme activity. The study provides insights into the biochemical properties of the enzymes and transporter, as well as their response to different conditions and effector concentrations.
Article
Biotechnology & Applied Microbiology
Rico Schwarz, Eric Zitzow, Adina Fiebig, Silvio Hering, Yvonne Humboldt, Nina Schoenwaelder, Neele Kampfer, Kerren Volkmar, Burkhard Hinz, Bernd Kreikemeyer, Claudia Maletzki, Tomas Fiedler
Summary: The study found that conjugation of SpyADI with PEG20 significantly reduced the overall activity of the enzyme without affecting its function in arginine. Furthermore, PEGylation increased the enzyme's anti-tumor capacity against GBM cells in vitro and led to a longer-lasting reduction of plasma arginine levels in mice.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Jing Tian, Daniel R. Utter, Lujia Cen, Pu-Ting Dong, Wenyuan Shi, Batbileg Bor, Man Qin, Jeffrey S. McLean, Xuesong He
Summary: This study reveals the function and impact of the acquired arginine deiminase system (ADS) in Saccharibacteria during the transition from the environment to the mammalian microbiome, and demonstrates its role in the mutualistic interaction and partner selection between Saccharibacteria and host bacteria.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Mohamed Gijon, Rachael L. Metheringham, Michael S. Toss, Samantha J. Paston, Lindy G. Durrant
Summary: The study found that high expression of cytoplasmic PADI2, PADI4, and nuclear PADI4 in colorectal cancer patients was associated with an increase in overall survival.
Review
Biochemistry & Molecular Biology
Yi Teng, Yuhang Chen, Xinyi Tang, Shengjun Wang, Kai Yin
Summary: In this review, the role of PAD2-mediated citrullination in tumor pathology and the regulation of tumor-associated immune cells is systematically illustrated. The article also discusses the feasibility of anti-PAD2 therapy to treat tumors and the urgent problems to be solved. Finally, recent developments in the development of PAD2 inhibitors are reviewed.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2023)
Article
Biochemistry & Molecular Biology
HanGoo Kang, Jinwon Seo, Eun-Jeong Yang, In-Hong Choi
Summary: Silver nanoparticles (AgNPs) are widely used for their antimicrobial properties, but they can also be harmful to both microorganisms and humans. Research has shown that 5 nm AgNPs can induce the formation of neutrophil extracellular traps (NETs), while 100 nm AgNPs cannot. The mechanism of AgNP-induced NETs involves reactive oxygen species (ROS), peptidyl arginine deiminase, and neutrophil elastase.
Article
Biochemistry & Molecular Biology
Sattaporn Weawsiangsang, Nontaporn Rattanachak, Touchkanin Jongjitvimol, Theerasak Jaifoo, Pensri Charoensit, Jarupa Viyoch, Sukunya Ross, Gareth M. Ross, Robert A. Baldock, Jirapas Jongjitwimol
Summary: This study is the first report that arginine deiminase (ADI)-related proteins are potential molecular targets for the inhibitory effect of hydroquinine against clinically isolated multidrug-resistant (MDR) P. aeruginosa strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Eleonora A. Starikova, Jennet T. Mammedova, Arina Ozhiganova, Tatiana A. Leveshko, Aleksandra M. Lebedeva, Alexey V. Sokolov, Dmitry V. Isakov, Alena B. Karaseva, Larissa A. Burova, Igor V. Kudryavtsev
Summary: In this study, the impact and mechanism of streptococcal arginine deiminase (ADI) on the function of human peripheral blood T lymphocytes were investigated. The results showed that ADI decreased arginine concentration, suppressed immune cell activity and altered cell differentiation state, as well as inhibited autophagy processes.
Article
Automation & Control Systems
V. A. Beloshenko, V. P. Plavan, N. M. Rezanova, B. M. Savchenko, I. Vozniak
INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TECHNOLOGY
(2019)
Article
Oncology
Lynn G. Feun, Ying-Ying Li, Chunjing Wu, Medhi Wangpaichitr, Patricia D. Jones, Stephen P. Richman, Beatrice Madrazo, Deukwoo Kwon, Monica Garcia-Buitrago, Paul Martin, Peter J. Hosein, Niramol Savaraj
Article
Mechanics
V. P. Plavan, V. G. Rezanova, Yu O. Budash, O. Ishchenko, N. M. Rezanova
MECHANICS OF COMPOSITE MATERIALS
(2020)
Article
Engineering, Manufacturing
Victor Beloshenko, Vyacheslav Chishko, Viktoria Plavan, Natalia Rezanova, Bogdan Savchenko, Nadiya Sova, Iurii Vozniak
Summary: The study investigates the influence of technological conditions and ZrO2 nanoparticles on the properties of fine-fibrous filter materials obtained through material extrusion. The use of different processing schemes and nanoadditives leads to a significant reduction in fiber diameter and improvement in microstructure integrity, resulting in enhanced air purification efficiency of the filter materials.
3D PRINTING AND ADDITIVE MANUFACTURING
(2021)
Review
Biochemistry & Molecular Biology
Medhi Wangpaichitr, George Theodoropoulos, Dan J. M. Nguyen, Chunjing Wu, Sydney A. Spector, Lynn G. Feun, Niramol Savaraj
Summary: The development of drug resistance in tumors is a significant complication to cancer chemotherapy, but targeting cisplatin-resistant tumors with new combination therapy may offer a major improvement. Recent research suggests that some tumors switch from glycolysis to oxidative metabolism, which is crucial for treatment strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Chunjing Wu, Min You, Dao Nguyen, Medhi Wangpaichitr, Ying-Ying Li, Lynn G. Feun, Macus T. Kuo, Niramol Savaraj
Summary: TRAIL and ADI-PEG20 are two drugs for treating melanoma, and their combination can enhance cytotoxicity to melanoma cells, including resistant ones. Studies show that combination therapy can alter the expression of cell signaling components in melanoma cells to promote cell death.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Mechanics
S. Saitarly, L. Dzubenko, V Plavan, Yu Pushkarev, A. Sapyanenko, P. Gorbyk
Summary: The presence of filler in polypropylene modified with a polyolefin elastomer compositions affects the structure, thermal stability, and technological characteristics. The study shows changes in the melting and crystallization temperatures with different filler concentrations, as well as an increase in strength and impact strength at low filler content.
MECHANICS OF COMPOSITE MATERIALS
(2022)
Article
Food Science & Technology
Olena Ishchenko, Viktoriia Plavan, Virgilijus Valeika, Maksym Koliada, Iryna Liashok, Yurii Budash, Volodymyr Bessarabov
Summary: The main objectives of this research are to study the properties of compositions made from starch or modified starch mixed with polyvinyl alcohol (PVA) in order to develop transdermal therapeutic films containing decamethoxine. The study investigates the influence of the content of carboxymethylated starch (CMS) in the compositions and films on their properties, and the effect of decamethoxine on the films. The results show that the polymer compositions have high thixotropic recovery and the content of decamethoxine affects the sorption properties of the films. The research suggests the possibility of developing effective pharmaceuticals using a solution-casting method with modified starch, PVA, and drugs.
Article
Biochemistry & Molecular Biology
Alexis D. Richaud, Mehdi Zaghouani, Guangkuan Zhao, Medhi Wangpaichitr, Niramol Savaraj, Stephane P. Roche
Summary: The conformational dynamics of PD1 are crucial for its binding ability, and this knowledge has led to the design of a new PD1 inhibitor.
Article
Engineering, Environmental
Yrii Budash, Viktoriia Plavan, Natalii Tarasenko, Olena Ishchenko, Maksym Koliada
Summary: This study investigated the effect of acid modification on the porous structure and adsorption properties of clays from the Cherkasy deposit. Acid modification improved the porous structure of clay samples and increased the surface area and pore volume. The sorption capacity was found to be influenced by the contact time and type of clay.
JOURNAL OF ECOLOGICAL ENGINEERING
(2023)
Article
Materials Science, Characterization & Testing
Yurii Budash, Natalia Rezanova, Viktoriia Plavan, Viktoriia Rezanova
Summary: The influence of the concentration of thermally and organomodified montmorillonite on structure formation in a blend of polypropylene/polystyrene (PP/PS) was studied. It was found that the content of the additive and the method of its modification affect the dimensional characteristics and distribution homogeneity of the microfibrillar structure. Thermally modified clay had a better modifying effect, improving the dimensional characteristics of PP microfibers at a minimum content. The formation of PP structures in the PS matrix and the relaxation of accumulated stresses were the main factors causing high values of the coefficient of swelling of the extrudates.
POLYMERS & POLYMER COMPOSITES
(2022)
Proceedings Paper
Materials Science, Multidisciplinary
D. Novak, Y. Budash, V Plavan, E. Kucherenko
Summary: This study investigates the effect of colloidal graphite in a polyethylene matrix on the electrical, mechanical, rheological, and optical properties of electroconductive compositions. It is found that increasing the volume content of the electroconductive filler decreases the electrical volume resistivity following an exponential law. Tensile strength and relative elongation decrease, while the melt flow index slightly increases with the increase of colloidal graphite concentration. Optical microscopic studies reveal both direct and indirect influences of colloidal graphite on the electrical volume resistivity and mechanical properties of the polyethylene compositions. At lower colloidal graphite contents, the material becomes more flexible due to better graphite dispersion, but stiffness significantly increases at higher colloidal graphite contents.
MATERIALS TODAY-PROCEEDINGS
(2022)
Article
Physiology
Yoel H. Sitbon, Francisca Diaz, Katarzyna Kazmierczak, Jingsheng Liang, Medhi Wangpaichitr, Danuta Szczesna-Cordary
Summary: This study evaluated the super relaxed state of myosin and sarcomeric protein phosphorylation in two pathological models of cardiomyopathy and a near-physiological model of cardiac hypertrophy. The mutations in the cardiomyopathy models had antagonistic effects on the myosin energetic states, leading to changes in RLC phosphorylation levels. The N-terminus of ELC was suggested to act as an energetic switch promoting the transition between SRX and DRX states, affecting myosin RLC phosphorylation.
JOURNAL OF GENERAL PHYSIOLOGY
(2021)
Article
Materials Science, Multidisciplinary
N. M. Rezanova, V. G. Rezanova, V. P. Plavan, O. O. Viltsaniuk
FUNCTIONAL MATERIALS
(2019)
Proceedings Paper
Polymer Science
I. Resnytskiy, O. Ishchenko, V. Plavan, M. Koliada, V. Valeika
INTERNATIONAL CONFERENCE BALTIC POLYMER SYMPOSIUM 2018
(2019)