Article
Biochemistry & Molecular Biology
Marie Cade, Javier Munoz-Garcia, Antoine Babuty, Louis Pare, Denis Cochonneau, Karim Fekir, Mathias Chatelais, Marie-Francoise Heymann, Anna Lokajczyk, Catherine Boisson-Vidal, Dominique Heymann
Summary: This study discovered that recombinant human FVIII has direct biological activities on endothelial cells, including decreased cell adhesion, regulation of transcriptomic and protein profiles, increased vascular permeability, and enhanced monocyte adhesion. The findings highlight new functions of FVIII in regulating vascular permeability and leukocyte adhesion and migration.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Virology
Jianda Li, Yuchen Li, Peng Liu, Xiuyu Wang, Yichao Ma, Qiu Zhong, Qian Yang
Summary: This study reveals the mechanisms of endothelial dysfunction in PEDV infection. Viral infection activates NECs and causes the upregulation of MMP-7 and proinflammatory cytokines. Using NECs, ECs, and PBMCs as in vitro models, we determined that the released MMP-7 contributed to the destruction of the endothelial barrier, and the released proinflammatory cytokines activated ECs to facilitate PBMCs migration. Moreover, the virus further exploited the migrated cells to promote viral dissemination. Thus, our results provide new insights into the mechanisms underlying endothelial dysfunction induced by coronavirus infection.
JOURNAL OF VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Max Laurens Bastiaan Gronloh, Janine Johanna Geertruida Arts, Eike Karin Mahlandt, Martijn A. Nolte, Joachim Goedhart, Jaap Diederik van Buul
Summary: During inflammation, primary neutrophil adhesion to the endothelium triggers the formation of transmigration hotspots, allowing secondary neutrophils to cross the endothelium more efficiently. This process is mediated by the activation of JNK1 and MARCKSL1, which induces the formation of filopodia and increases neutrophil adhesion.
Article
Biology
Boyang Ren, Huan Xia, Yijun Liao, Hang Zhou, Zhongnan Wang, Yaoyao Shi, Mingzhao Zhu, Juan Carlos Zuniga-Pfluecker
Summary: This study reveals a novel role of endothelial SIRP alpha signaling for thymic HPC homing for T cell development.
Article
Biochemistry & Molecular Biology
Max L. B. Gronloh, Janine J. G. Arts, Sebastian Palacios Martinez, Amerens A. van Der Veen, Lanette Kempers, Abraham C. van Steen, Joris J. T. H. Roelofs, Martijn A. Nolte, Joachim Goedhart, Jaap D. van Buul
Summary: Using a photoconvertible probe, researchers traced and identified the original endothelial transmigration hotspots, and found that these hotspots function to limit vascular leakage during transmigration events. The distribution of ICAM-1 determines the location of the transmigration hotspot.
Article
Biochemistry & Molecular Biology
Thomas Brock, Elisabeth Boudriot, Anke Klawitter, Marianne Grosser, Trang T. P. Nguyen, Sindy Giebe, Erik Klapproth, Achim Temme, Ali El-Armouche, Georg Breier
Summary: VE-cadherin enhances the adhesion of breast cancer cells to endothelial cells but does not affect their transmigration, indicating that cancer cell adhesion and incorporation are distinct processes governed by different molecular mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Ricardo Huilcaman, Natalia Veliz-Olivos, Whitney Venturini, Alexandra Olate-Briones, Adriana Treuer, Claudio Valenzuela, Nelson Brown, Rodrigo Moore-Carrasco
Summary: The study found that platelets have the ability to migrate during atherosclerosis, and factors secreted by monocytes can enhance the migratory and aggregating capabilities of platelets.
Article
Medicine, Research & Experimental
Cong Zhang, Zhuowen Yang, Peng Zhou, Muxin Yu, Baorong Li, Yingmiao Liu, Jiaqi Jin, Wenhui Liu, Haijiao Jing, Jingwen Du, Jie Tian, Zhiyu Zhao, Jianxin Wang, Yinzhu Chu, ChunMei Zhang, Valerie A. Novakovic, Jialan Shi, Changjun Wu
Summary: The study found that neoadjuvant chemotherapy significantly increased the expression of PS+ BCMPs in patient plasma, which was associated with a rapid increase in procoagulant activity. Treatment with lactadherin reduced the adhesion of BCMPs and their procoagulant activity. Injection of BCMPs in mice induced a hypercoagulable state, reducing fibrinogen levels and promoting thrombus formation.
Review
Oncology
Katerina Jerabkova-Roda, Amandine Dupas, Nael Osmani, Vincent Hyenne, Jacky G. Goetz
Summary: The intravascular behavior of tumor-derived extracellular vesicles (EVs) and circulating tumor cells (CTCs) plays a crucial role in the metastatic cascade. Understanding the role of cellular adhesion molecules (CAMs) and their endothelial ligands in dictating the arrest site and exit capacity of EVs/CTCs could provide insight into novel targets for therapies. Exploring the differences and similarities between CAMs on CTCs and EVs may help in understanding their role in organotropism of different cancer types.
Article
Virology
Xinyue Yao, Wanwan Dai, Siyu Yang, Zhaoli Wang, Qian Zhang, Qinghui Meng, Tao Zhang
Summary: Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) can infect porcine pulmonary microvascular endothelial cells (MVECs) and damage the endothelial glycocalyx. This study further explored the synergistic effect of HP-PRRSV and lipopolysaccharide (LPS) on the inflammatory response of MVECs. The results provide insights into the mechanism of severe lung injury caused by secondary bacterial infection following HP-PRRSV infection.
Article
Biology
Janine J. G. Arts, Eike K. Mahlandt, Max L. B. Gronloh, Lilian Schimmel, Ivar Noordstra, Emma Gordon, Abraham C. van Steen, Simon Tol, Barbara Walzog, Jos van Rijssel, Martijn A. Nolte, Marten Postma, Satya Khuon, John M. Heddleston, Eric Wait, Teng Leong Chew, Mark Winter, Eloi Montanez, Joachim Goedhart, Jaap D. van Buul
Summary: During inflammation, leukocytes rapidly transmigrate across the endothelium by choosing membrane protrusions at cell junctions, which depend on Rac1 activity. They cross the junction by migrating underneath the protruding endothelial cell, facilitating their exit from inflamed vessel walls.
Article
Microbiology
Zhaolei Zou, Juan Fang, Wanting Ma, Junyi Guo, Zhongyan Shan, Da Ma, Qiannan Hu, Liling Wen, Zhi Wang
Summary: In this study, it was found that gingipains from P. gingivalis can degrade PECAM-1 to facilitate bacterial penetration and at the same time reduce leukocyte TEM capacity. The role of gingipains in modulating vascular barrier permeability and TEM processes provides a new understanding of the distal colonization of P. gingivalis and its associated systemic diseases.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Qi Zhang, Changli Wang, Yufei Wu, Jingjing Liu, Tianyi Wang, Bing Wang
Summary: This study investigated the functions and molecular mechanism of BAP31 on the miR-206/133b cluster in colorectal cancer (CRC). The results showed that BAP31 overexpression led to a reduction in the expression of the miR-206/133b cluster, which was correlated with the transendothelial migration capability of CRC cells. The miR-206/133b cluster was found to directly regulate cell division cycle 42 (CDC42) and actin-related protein 2/3 complex subunit 5 (ARPC5) in the tight junction pathway (hsa04530), and the potential transcription regulator of the miR-206/133b cluster was identified as Homeobox D10 (HOXD10). The study further elucidated the molecular mechanisms and functional mechanisms of BAP31's regulatory role in the expression levels of the miR-206/133b cluster by inhibiting HOXD10 translocation from the cytoplasm to the nucleus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cardiac & Cardiovascular Systems
Junqi Wang, Xiaoping Chen
Summary: This review summarizes the roles of JAMs in the development of atherosclerosis and predicts future research directions.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Immunology
Kim C. M. Jeucken, Charlotte C. N. van Rooijen, Yik Y. Kan, Lotte A. Kocken, Aldo Jongejan, Abraham C. van Steen, Jaap D. van Buul, Henric K. Olsson, Jan Piet van Hamburg, Sander W. Tas
Summary: The study shows that CD4(+) memory T cells can induce inflammatory responses in endothelial cells, and inhibition of NF-kappa B signaling can modulate these responses. Moreover, pharmacological inhibitors of NF-kappa B signaling can potentiate the effects of current therapies for IMIDs on endothelial cell inflammation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mobarak Abu Mraheil, Haroldo A. Toque, Luigi La Pietra, Juerg Hamacher, Tenzing Phanthok, Alexander Verin, Joyce Gonzales, Yunchao Su, David Fulton, Douglas C. Eaton, Trinad Chakraborty, Rudolf Lucas
Summary: Pneumococci promote colonization by producing high levels of H2O2 and have unique mechanisms to protect themselves from its damage. The production of H2O2 forms an important virulence phenotype and overlaps with the activities of other virulence factors.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Article
Pharmacology & Pharmacy
Lina Wang, Xiaoyu Zhang, Yapeng Cao, Qian Ma, Xiaoxiao Mao, Jiean Xu, Qiuhua Yang, Yaqi Zhou, Rudolf Lucas, David J. Fulton, Yunchao Su, Scott A. Barman, Mei Hong, Zhiping Liu, Yuqing Huo
Summary: The study demonstrates that the reduced expression of glycolytic molecules in lung macrophages due to Pfkfb3 deficiency or inhibition can effectively alleviate the development of pulmonary hypertension, with lower levels of growth factors and pro-inflammatory cytokines produced by alveolar macrophages.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Harald Hundsberger, Anna Stierschneider, Victoria Sarne, Doris Ripper, Jasmin Schimon, Hans Peter Weitzenbock, Dominik Schild, Nico Jacobi, Andreas Eger, Josef Atzler, Christian T. Klein, Christoph Wiesner
Summary: Quercetin has different effects on 2D and 3D melanoma models, with higher concentrations reducing melanoma growth and lower concentrations promoting cancer in metastatic melanoma spheroids. The regulation of Nrf2/ARE-mediated cytoprotective genes, as well as ERK and NF-kappa B phosphorylation, may play a key role in determining the tumor promoting or repressing effects in metastatic melanoma spheroids. Further studies are needed to understand the mechanisms underlying the pro- and anti-carcinogenic effects of quercetin on human melanomas.
Review
Food Science & Technology
Joyce Gonzales, Trinad Chakraborty, Maritza Romero, Mobarak Abu Mraheil, Abdullah Kutlar, Betty Pace, Rudolf Lucas
Summary: Sickle cell disease is a common genetic disorder that impairs immunity and can lead to complications like acute chest syndrome. Infections with Streptococcus pneumoniae pose a particular danger to patients, as the bacterium produces toxins that can damage the lungs.
Article
Medicine, Research & Experimental
Benedikt Schmid, Markus Kredel, Roman Ullrich, Katharina Krenn, Rudolf Lucas, Klaus Markstaller, Bernhard Fischer, Peter Kranke, Patrick Meybohm, Bernhard Zwissler, Sandra Frank
Summary: This study presents a protocol for a Phase IIB clinical trial investigating the safety and potential efficacy of a novel inhaled peptide agent in ARDS patients. The randomized, placebo-controlled, double-blind intervention study will involve patients with moderate to severe ARDS requiring mechanical ventilation. The substantial data collection, short patient inclusion timeframe, and focus on informed consent are important considerations for the successful implementation of this trial.
Article
Oncology
Hans Peter Weitzenboeck, Anna Gschwendtner, Christoph Wiesner, Maren Depke, Frank Schmidt, Franz Trautinger, Markus Hengstschlaeger, Harald Hundsberger, Mario Mikula
Summary: The mechanisms of how NRF2 contributes to melanoma progression are not fully understood, but inhibition of NRF2 has been found to induce an EMT phenotype and increase cell survival after apoptosis induction. Cells lacking NRF2 show increased cell viability after specific treatment, and CD44 expression is crucial for survival upon a certain treatment.
Article
Biochemistry & Molecular Biology
Hanno Huwer, Yalda Hadizamani, Ueli Moehrlen, Uz Stammberger, Florian Gebhard, Lia Bally, Albrecht Wendel, Ulrich C. Liener, Rudolf Lucas, Juerg Hamacher
Summary: The study investigates the role of post-trauma-induced oedema generation and the potential benefits of beta 2-adrenergic receptors agonists in blast-induced lung injury. The results suggest that beta 2-adrenoceptor stimulation has a beneficial impact on oedema generation, while amiloride exacerbates the condition.
Article
Multidisciplinary Sciences
Juerg Hamacher, Yalda Hadizamani, Hanno Huwer, Ueli Moehrlen, Lia Bally, Uz Stammberger, Albrecht Wendel, Rudolf Lucas
Summary: This study aimed to establish a sequence of alterations in blast-induced lung injury in rats using an advanced blast generator. The results showed that after blast lung injury, significant haemorrhage and neutrophil infiltration occurred, as well as increased levels of pro-inflammatory cytokines and decreased levels of anti-inflammatory cytokines. Pulmonary oedema developed but recovered within 24 hours.
Article
Cell Biology
Katrin Colleselli, Marie Ebeyer-Masotta, Benjamin Neuditschko, Anna Stierschneider, Christopher Pollhammer, Mia Potocnjak, Harald Hundsberger, Franz Herzog, Christoph Wiesner
Summary: The interaction between monocytes and endothelial cells plays a key role in inflammation, including chemoattraction, adhesion, and transendothelial migration. Toll-like receptor 2 (TLR2) is critical for sensing pathogens and initiating an immune response. In this study, we found that TLR2 promotes monocyte adhesion to endothelial cells, disrupts the endothelial barrier, and affects actin polymerization. Our findings also revealed the association of TLR2 with specific integrins and identified novel proteins affected by TLR2.
Article
Cell Biology
Anna Stierschneider, Benjamin Neuditschko, Katrin Colleselli, Harald Hundsberger, Franz Herzog, Christoph Wiesner
Summary: Optogenetic cell lines based on light-oxygen-voltage-sensing (LOV) domains (opto-TLR4-LOV LECs and opto-TLR4-LOV HUVECs) allow fast, precise, and reversible activation of TLR4 signaling pathways, providing a better simulation of inflammatory responses than LPS. Light-induced TLR4 activation can promote the expression of inflammatory proteins and significantly impact cell function and cell migration. This technology enables specific studies of TLR4.
Review
Biochemistry & Molecular Biology
Katrin Colleselli, Anna Stierschneider, Christoph Wiesner
Summary: While inflammation is crucial for human survival in response to infection and injury, prolonged inflammation can be fatal. Toll-like receptors (TLRs) are important in initiating inflammatory processes, and TLR2, in particular, has been extensively studied and known to recognize a wide range of pathogens through heterodimerization. However, many unanswered questions still remain about the mechanisms of TLR2 in both health and disease. This review provides an up-to-date overview of TLR2, including its homo- and heterodimers, and discusses its pro- and anti-inflammatory properties and recent findings in infectious and neurodegenerative diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Jasmin Bazant, Benjamin Ott, Martina Hudel, Torsten Hain, Rudolf Lucas, Mobarak Abu Mraheil
Summary: H2O2 negatively affects the activity and release of PLY. Decreased H2O2 production leads to a decline in PLY release, possibly due to the upregulation of certain genes.
Article
Biochemistry & Molecular Biology
Pau Martin-Malpartida, Silvia Arrastia-Casado, Josep Farrera-Sinfreu, Rudolf Lucas, Hendrik Fischer, Bernhard Fischer, Douglas C. Eaton, Susan Tzotzos, Maria J. Macias
Summary: This article introduces the potential mechanism of solnatide as a novel therapeutic agent for treating pulmonary edema. By mimicking the action of human TNF, solnatide can activate ENaC, thereby enhancing alveolar fluid clearance. Through NMR analysis, researchers found that solnatide has a folding structure with a small hydrophobic core and two charged polarity regions. They propose a model for the interaction between solnatide and the C-terminal domain of the ENaCα subunit, which can guide future experiments and the design of new drugs.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
