Article
Biochemistry & Molecular Biology
Jun Rao, Xianghui Wan, Fangfang Tou, Qinsi He, Aihua Xiong, Xinyi Chen, Wenhao Cui, Zhi Zheng
Summary: This study used proteomics and metabolomics techniques to analyze serum samples from patients with advanced CRC, identifying potential biomarkers and pathways associated with cancer cell metabolism. The results suggest the conservation role of lipids in the regulatory network of advanced CRC and discovered novel biomarkers that can improve diagnosis and monitoring of advanced CRC.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Chemistry, Analytical
Jia Yi, Yueqing Shen, Yi Yang, Chengpin Shen, Baohong Liu, Liang Qiao, Yan Wang
Summary: By characterizing gingival crevicular fluid (GCF) sediments and identifying potential protein biomarkers using mass spectrometry techniques, this study has contributed to the early diagnosis of periodontitis and understanding of its pathogenesis. The identified biomarkers hold potential value for accurate monitoring and can be utilized by non-specialists for widespread screening.
Review
Chemistry, Analytical
Negin Fasih Ramandi, Mohammad Faranoush, Alireza Ghassempour, Hassan Y. Aboul-Enein
Summary: The recent success in studying the proteome as a source of biomarkers has revolutionized our understanding of leukemia. The use of mass spectrometry techniques to identify differentially expressed proteins has led to improved diagnosis, prognosis, and treatment strategies for leukemia. This review focuses on the use of various ionization sources and mass analyzers in monitoring different types of leukemia, with MALDI-TOF MS identified as a quick and reliable method for protein analysis. Additionally, the review classifies over 400 proteins and identifies specific biomarkers for acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), and other stages of leukemia for future studies.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Francesca Scebba, Stefano Salvadori, Silvia Cateni, Paola Mantellini, Francesca Carozzi, Simonetta Bisanzi, Cristina Sani, Marzia Robotti, Ivana Barravecchia, Francesca Martella, Valentina Colla, Debora Angeloni
Summary: Ovarian cancer (OC) is a deadly gynecological cancer, and early diagnosis is crucial for a cure. Currently, a reliable screening method for early detection of OC is lacking. In this study, saliva samples were analyzed using proteomics to identify candidate biomarkers for OC. The results show that saliva is an informative test fluid for early detection of OC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Liang Shan, Han Gao, Jing Zhang, Wentao Li, Yue Su, Yinlong Guo
Summary: This study combined MALDI-TOF MS with EM technology to directly analyze exosomal proteins in human plasma and serum. The results showed that EM had higher sensitivity in the high-mass range and characteristic exosomal proteins were identified. Furthermore, when using EM as a detector, there was a good linear relationship between peak intensity and concentration.
Article
Immunology
Zhi Zheng, Qingfeng Wei, Xianghui Wan, Xiaoming Zhong, Lijuan Liu, Jiquan Zeng, Lihua Mao, Xiaojian Han, Fangfang Tou, Jun Rao
Summary: In this study, integrated proteomics and metabolomics analyses were performed on serum samples from advanced CRC patients. Significant alterations in proteins and metabolites were found after treatment. The proteomics analysis identified changes in cancer-related pathways and immune response pathways, while the metabolomics analysis revealed changes in pathways associated with lipid metabolism. The results of this study not only expanded our understanding of the molecular basis of advanced CRC but also provided potential novel therapeutic targets for CRC treatment.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Endocrinology & Metabolism
Wenjie Zhou, Shanze Li, Guijiang Sun, Lili Song, Wenjun Feng, Rui Li, Hui Liu, Yaqian Dong, Siyu Chen, Shenshen Yang, Jing Li, Yubo Li
Summary: This study identified potential biomarkers in the serum of atherosclerosis-induced ischemic stroke (IS) patients and combined them with the atherosclerotic index (AI) to provide early warning for the diagnosis of IS. Changes in glycerophospholipid metabolism may indicate an early risk of IS development.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Immunology
Joanna Matysiak, Eliza Matuszewska, Marek L. Kowalski, Slawomir W. Kosinski, Ewa Smorawska-Sabanty, Jan Matysiak
Summary: Venom immunotherapy (VIT) in allergic patients is closely associated with changes in serum proteomic patterns, particularly involving complement proteins and myosin, with major proteomic changes detected in high responder group, suggesting a direct response to VIT.
Article
Microbiology
David Kornspan, Holger Brendebach, Dirk Hofreuter, Shubham Mathur, Shlomo Eduardo Blum, Marcelo Fleker, Svetlana Bardenstein, Sascha Al Dahouk
Summary: Researchers identified unique biomarkers using proteomics comparison to distinguish different Brucella strains, validating them with MALDI-TOF MS technology. They found two genus-wide unique biomarkers that effectively discriminated the Rev.1 vaccine strain from field isolates.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Heba Badr, Ron Blutrich, Kaitlin Chan, Jiefei Tong, Paul Taylor, Wen Zhang, Ran Kafri, Hannes L. Rost, Ming-Sound Tsao, Michael F. Moran
Summary: Proteome analysis revealed co-expressed upregulated metabolism proteins highly conserved between primary NSCLC and patient-derived xenograft tumors. The encoded genes of these proteins are associated with the survival of NSCLC patients, suggesting their role in driving NSCLC through metabolic processes.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Katarina Davalieva, Sanja Kiprijanovska, Ognen Ivanovski, Aleksandar Trifunovski, Skender Saidi, Aleksandar Dimovski, Zivko Popov
Summary: The high recurrence rate and invasive diagnostic methods in bladder cancer clinical management require the development of new non-invasive molecular tools for early detection and risk stratification. This study identified potential non-invasive diagnostic and prognostic markers using a new analysis method, expanding the field of bladder cancer research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Congqi Hu, Zhao Dai, Jia Xu, Lianyu Zhao, Yanping Xu, Meilin Li, Jiahui Yu, Lu Zhang, Hui Deng, Lijuan Liu, Mingying Zhang, Jiarong Huang, Linping Wu, Guangxing Chen
Summary: This study identified the serum marker ORM1 as a significant differential expression between normal and RA patient samples, positively correlated with disease activity, offering a new perspective for future research on RA.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Yong-Min Kim, Sung-Woo Park, Mi-Jin Lee, Da-Yeon Jeon, Su-Jin Sa, Yong-Dae Jeong, Ha-Seung Seong, Jung -Woo Choi, Shinichi Hochi, Eun-Seok Cho, Hak-Jae Chung
Summary: In this study, the iTRAQ technique and LC-MS/MS analysis were used to identify differentially expressed CM10-fractionated proteins in fresh and frozen-thawed boar semen. The results showed that 9 proteins were upregulated and 67 proteins were downregulated in frozen-thawed samples. These proteins are involved in oxidative phosphorylation, mitochondrial inner membrane and matrix, and pyruvate metabolic processes.
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2023)
Article
Medical Laboratory Technology
Guisheng Zhou, Peng Wei, Jinshan Lan, Qiongzi He, Feng Guo, Yunke Guo, Wanjian Gu, Tingting Xu, Shijia Liu
Summary: This study compared the protein profiles of healthy individuals and SLE patients to reveal the pathogenesis of SLE and identify specific biomarkers for diagnosis. SAA1 and CD248 were identified as reliable biomarkers for early immune diagnosis of SLE.
CLINICA CHIMICA ACTA
(2022)
Article
Food Science & Technology
Xue Li, Xiaorong Deng, Xin Guo, Yabo Wei, Yunfeng Zhao, Xiaobing Guo, Xinrong Zhu, Jian Zhang, Ling Hu
Summary: The present study investigates the freshness indicator protein of Coregonus peled and the effects of oxidation on the quality of fish during 4 degrees C storage. A total of 382 differential proteins were identified, and 20 proteins related to oxidation were found to have the potential as biomarkers for protein oxidative damage. Actin was identified as an indicator for fish softening during postmortem storage. The changes in protein structure and biomarkers were influenced by oxidation. Bioinformatics analysis suggested that differentially expressed proteins participated in the metabolic pathway during 4 degrees C storage.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)