期刊
BRIEFINGS IN FUNCTIONAL GENOMICS
卷 9, 期 2, 页码 129-138出版社
OXFORD UNIV PRESS
DOI: 10.1093/bfgp/elp060
关键词
fluorescent proteins; maize; Arabidopsis; high-throughput techniques; global expression; functional profiling
资金
- National Science Foundation [DBI 0501862]
- Direct For Biological Sciences
- Div Of Biological Infrastructure [0963400] Funding Source: National Science Foundation
First discovered in jellyfish, fluorescent proteins (FPs) have been successfully optimized for use as effective biomarkers within living plant cells. When exposed to light, FPs fused to a protein or regulatory element will fluoresce, and non-invasively mark expression and protein localization, which allows for the in vivo monitoring of diverse cellular processes. In this review, we discuss how FP technology has evolved from small-scale analysis of individual genes to more high-throughput techniques for global expression and functional profiling in plants.
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