期刊
BREEDING SCIENCE
卷 64, 期 4, 页码 321-330出版社
JAPANESE SOC BREEDING
DOI: 10.1270/jsbbs.64.321
关键词
genetic diversity; allelic variation; Brassica oleracea; resynthesized Brassica napus; SSR mutation
资金
- National Science & Technology Pillar Program during the Twelfth Five-year Plan Period [2011BAD35B09]
- Chengguang Program for Young Scientists of Wuhan Municipal Government [2013070104010031]
- National Natural Science Foundation of China [31301360, 31101124]
- Strategic Japanese-Chinese Cooperative Program on Climate Change [2012DFG90290]
Assessing the level of genetic diversity within a germplasm collection contributes to evaluating the potential for its utilization as a gene pool to improve the performance of cultivars. In this study, 45 high-quality simple sequence repeat (SSR) markers were screened and used to estimate the genetic base of a world-wide collection of 248 rapeseed (Brassica Papas.) inbred lines. For the whole collection, the genetic diversity of A genorne was higher than that of C genome. The genetic diversity of C genome for the semi-winter type was the lowest among the different germplasm types. Because B. oleracea is usually used to broaden the genetic diversity of C genome in rapeseed, we evaluated the potential of 25 wild B. oleracea lines. More allelic variations and a higher genetic diversity were observed in B. oleracea than in rapeseed. One B. oleracea line and one oilseed B. rapa line were used to generate a resynthesized Brassica napus line, which was then crossed with six semi-winter rapeseed cultivars to produce 7 F-1 hybrids. Not only the allele introgression but also mutations were observed in the hybrids, resulting in significant improvement of the genetic base.
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