Article
Oncology
Lyvianne Decourtye-Espiard, Nicola Bougen-Zhukov, Tanis Godwin, Tom Brew, Emily Schulpen, Michael A. Black, Parry Guilford
Summary: This study discovered that inactivating mutations in the CDH1 gene lead to heightened sensitivity of gastric and breast cancers to histone deacetylase (HDAC) inhibitors. One specific inhibitor, entinostat, showed strong inhibitory effects on models with CDH1 mutations. This research highlighted the potential beneficial impact of entinostat for chemoprevention and/or treatment of cancers with CDH1 mutations.
Article
Ophthalmology
Jiayi Wei, Liangjing Wu, Shuai Yang, Conghui Zhang, Le Feng, Minli Wang, Hui Li, Fang Wang
Summary: Proliferative vitreoretinopathy (PVR) is a serious ocular fibrotic disease that can lead to blindness. This study focuses on the pathogenesis of PVR and the mechanism behind the epithelial-mesenchymal transition (EMT) in retinal pigment epithelial (RPE) cells. The results show that the loss of E-cadherin leads to changes in the catenin-cadherin complex, activating zinc finger E-box binding homeobox 1 (ZEB1) and promoting the upregulation of N-cadherin. Overexpression of E-cadherin inhibits the EMT process and cell proliferation in RPE cells.
EXPERIMENTAL EYE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Iason Psilopatis, Jule Ida Schaefer, Dimitrios Arsenakis, Dimitrios Bolovis, Georgia Levidou
Summary: This study provides preliminary evidence suggesting that overexpression of SOX11 might promote EMT in metastatic serous ovarian cancer, thereby enhancing tumor metastasis.
Article
Biochemistry & Molecular Biology
Xiaoru Zhang, Vinay Singh Tanwar, Cynthia C. Jose, Hyun-Wook Lee, Suresh Cuddapah
Summary: This study demonstrates that nickel exposure leads to persistent downregulation of E-cadherin by causing loss of Sp1 binding at the CDH1 promoter, which induces EMT. Continuous upregulation of ZEB1 is also a key factor in the loss of E-cadherin, and knocking out ZEB1 using CRISPR-Cas9 technology can restore E-cadherin expression.
MOLECULAR CARCINOGENESIS
(2022)
Article
Biochemistry & Molecular Biology
Aikaterini Stavrou, Angelica Ortiz, Max Costa
Summary: Cadmium exposure, even at low doses, can lead to lung and kidney cancers in both humans and animals. This study focuses on how cells exposed to low doses of cadmium are able to survive and proliferate by activating the EGFR/STAT5 pathway, promoting gene expression associated with cell survival and proliferation. The results demonstrate increased expression of EGFR and STAT5 proteins, as well as a decrease in E-cadherin expression, suggesting that cadmium-induced toxicity can be overcome through activation of the EGFR pathway.
Article
Medicine, Research & Experimental
Zewei Wang, Jingtian Lai, Yu Li, Haiying Zhou, Ahmad Alhaskawi, Pengfei Li, Xinyuan Shen, Hui Lu, Tian Tu
Summary: After skin injury, the re-epithelialization process is crucial for healing, but if the skin appendages are damaged, the process becomes vulnerable. Researchers are exploring the use of pluripotent stem cells from other tissue sources to create a functionalized epithelium that can rapidly close wounds without causing further damage. Human adipose-derived stem cells (hASCs) are a potential source, but effective induction protocols have not been developed yet. Over-expression of E-cadherin in hASCs could potentially induce the epithelial phenotype and make them viable for wound re-epithelialization and skin tissue engineering efforts.
MEDICAL HYPOTHESES
(2023)
Article
Medicine, Research & Experimental
Vignesh Ramesh, Paradesi Naidu Gollavilli, Luisa Pinna, Mohammad Aarif Siddiqui, Adriana Martinez Turtos, Francesca Napoli, Yasmin Antonelli, Aldo Leal-Egana, Jesper Foged Havelund, Simon Toftholm Jakobsen, Elisa Le Boiteux, Marco Volante, Nils Joakim Faergeman, Ole N. Jensen, Rasmus Siersbaek, Kumar Somyajit, Paolo Ceppi
Summary: This study found an inverse association between short-chain fatty acids and EMT in non-small cell lung cancer patients. In vitro experiments showed that propionate treatment enhanced the epithelial transcriptional program and reduced the EMT phenotype in lung cancer cell lines. Animal experiments also confirmed that propionate can reduce lung cancer metastasis and lymph node spread. Further mechanistic investigation revealed that propionate treatment caused chromatin remodeling through p300-mediated histone acetylation.
EMBO MOLECULAR MEDICINE
(2023)
Article
Oncology
Meng Luo, Jinfan Li, Qi Yang, Song Xu, Kun Zhang, Jing Chen, Suzhan Zhang, Shu Zheng, Jiaojiao Zhou
Summary: This study identifies N4BP3 as a new regulator that promotes breast cancer metastasis by regulating the ubiquitination and degradation of E-cadherin. It uncovers an unprecedented role for N4BP3 in breast cancer metastasis and elucidates the underlying molecular mechanisms.
Article
Chemistry, Multidisciplinary
Miaomiao Zhang, Juan Qin, Xinyue Guo, Zongjia Li, Christian Rankl, Bailin Zhang, Jilin Tang
Summary: During tumor-associated epithelial-mesenchymal transition (EMT), the expression of the EMT marker E-cadherin is challenging to detect on cancer cell surfaces in the middle and late stages of EMT. This study investigated the changes in trace E-cadherin on the living bladder cancer T24 cell surface during EMT using force-distance curve-based atomic force microscopy. The results showed that T24 cells remain in an intermediate state and can be transformed into the mesenchymal phenotype through long-term TGF-β1 induction.
Article
Agriculture, Dairy & Animal Science
Alessandro Sammarco, Chiara Gomiero, Giorgia Beffagna, Laura Cavicchioli, Silvia Ferro, Silvia Michieletto, Enrico Orvieto, Marco Patruno, Valentina Zappulli
Summary: This study investigated the expression of molecules related to epithelial-to-mesenchymal transition (EMT) in human breast cancer, canine mammary tumors, and feline mammary tumors at the gene and protein level. It was found that some typical EMT markers were not highly expressed at the mRNA level in tumors, suggesting the need for investigation of other markers. However, at the protein level, molecules such as vimentin and E-cadherin were associated with higher aggressiveness and potential usefulness as markers. The study also confirmed similarities between feline mammary tumors and triple negative breast cancer, as well as between canine mammary tumors and the less aggressive subtype of human breast cancer that expresses hormonal receptors.
Article
Oncology
Yuxiang Liu, Taolin Chen, Mingyue Guo, Yu Li, Qian Zhang, Guixiang Tan, Li Yu, Yongjun Tan
Summary: FOXP2, a language-related gene, interacts with FOXA2 in breast cancer cells to inhibit EMT by activating the transcription of certain genes like E-cadherin and PHF2. The results suggest a collaborative role of FOXP2 and FOXA2 in regulating EMT in breast cancer cells.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Leonardo Franz, Lorenzo Nicole, Anna Chiara Frigo, Giancarlo Ottaviano, Piergiorgio Gaudioso, Tommaso Saccardo, Francesca Visconti, Rocco Cappellesso, Stella Blandamura, Ambrogio Fassina, Gino Marioni
Summary: The mechanism of epithelial-mesenchymal transition (EMT) plays a crucial role in cancer development, tumor progression, and therapy resistance. In laryngeal carcinoma, the predictive value of pN+ status and Slug expression on disease-free survival was identified, supporting the hypothesis of a mutual concurrence of EMT and angiogenesis in driving aggressive phenotype in LSCC. Further studies are needed to characterize the predictive performance of prognostic models based on EMT and angiogenesis.
Article
Biology
Qing Zhang, Feng Lin, Jianyong Huang, Chunyang Xiong
Summary: Cell phenotype heterogeneity within tumor tissue, especially due to epithelial-mesenchymal transition (EMT), plays a role in cancer invasion and metastasis. In this study, heterotypic tumor spheroids containing both epithelial and EMT cancer cells were generated and it was found that EMT cells dominated the periphery of the spheroid. Furthermore, EMT cells acted as leader cells to enhance the migration efficiency of epithelial cancer cells and promote dispersion and invasion of the spheroids through force transition and heterophilic N-cadherin/E-cadherin adhesion complexes. Impairment of this adhesion complex formation prevented EMT cells from guiding epithelial cancer cell migration and blocked the dispersion of tumor spheroids.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Medicine, Research & Experimental
Yang Fan, Zijie Gao, Jianye Xu, Huizhi Wang, Qindong Guo, Boyan Li, Ming Li, Hao Xu, Yanhua Qi, Shulin Zhao, Wei Qiu, Ziwen Pan, Qingtong Wang, Hao Xue, Rongrong Zhao, Xing Guo, Gang Li
Summary: This study found that targeting SPI1 to block transcription of the MIR222HG cluster helps reduce radioresistance and combat the immunosuppressive microenvironment in GBM. PLX-4720 is a potential GBM drug and radiosensitizer.
Article
Chemistry, Multidisciplinary
Ying Han, Cuicui Qiu, Jiaojiao Li, Fuping Gao, Qing Yuan, Yuhua Tang, Wenchao Niu, Xiayan Wang, Xueyun Gao, Liang Gao
Summary: The study introduces a metal cluster-based electrochemical biosensing system to determine the expressing levels of N-cadherin during the EMT process of tumor cells, providing a rapid, sensitive, and reliable whole-cell biosensor integrating fluorescence and electrochemical signals.
Article
Oncology
Amanda J. Schech, Preeti Shah, Stephen Yu, Gauri J. Sabnis, Olga Goloubeva, Paula Rosenblatt, Armina Kazi, Saranya Chumsri, Angela Brodie
BREAST CANCER RESEARCH AND TREATMENT
(2015)
Article
Oncology
Amanda Schech, Stephen Yu, Olga Goloubeva, John McLenithan, Gauri Sabnis
ENDOCRINE-RELATED CANCER
(2015)
Article
Oncology
Michael D. Curley, Gauri J. Sabnis, Lucia Wille, Bambang S. Adiwijaya, Gabriela Garcia, Victor Moyo, Armina A. Kazi, Angela Brodie, Gavin MacBeath
MOLECULAR CANCER THERAPEUTICS
(2015)
Article
Oncology
Amanda Schech, Armina Kazi, Stephen Yu, Preeti Shah, Gauri Sabnis
MOLECULAR CANCER THERAPEUTICS
(2015)
Article
Cell Biology
Laszlo Prokai, Vien Nguyen, Szabolcs Szarka, Puja Garg, Gauri Sabnis, Heather A. Bimonte-Nelson, Katie J. McLaughlin, Joshua S. Talboom, Cheryl D. Conrad, Paul J. Shughrue, Todd D. Gould, Angela Brodie, Istvan Merchenthaler, Peter Koulen, Katalin Prokai-Tatrai
SCIENCE TRANSLATIONAL MEDICINE
(2015)
Article
Multidisciplinary Sciences
Istvan Merchenthaler, Malcolm Lane, Gauri Sabnis, Angela Brodie, Vien Nguyen, Laszlo Prokai, Katalin Prokai-Tatrai
SCIENTIFIC REPORTS
(2016)
Article
Oncology
Rabia A. Gilani, Armina A. Kazi, Preeti Shah, Amanda J. Schech, Saranya Chumsri, Gauri Sabnis, Anil K. Jaiswal, Angela H. Brodie
BREAST CANCER RESEARCH AND TREATMENT
(2012)
Article
Oncology
Gauri J. Sabnis, Armina Kazi, Olga Golubeva, Preeti Shah, Angela Brodie
BREAST CANCER RESEARCH AND TREATMENT
(2013)
Article
Pharmacology & Pharmacy
Susan B. Kesmodel, Gauri J. Sabnis, Saranya Chumsri, Angela M. H. Brodie
CURRENT PHARMACEUTICAL DESIGN
(2014)
Review
Oncology
Saranya Chumsri, Amanda Schech, Chakkapong Chakkabat, Gauri Sabnis, Angela Brodie
EXPERT REVIEW OF ANTICANCER THERAPY
(2014)
Review
Oncology
Saranya Chumsri, Gauri Sabnis, Katherine Tkaczuk, Angela Brodie
Article
Biochemistry & Molecular Biology
Jun H. Lee, Gauri Sabnis, Anjan Nan
MACROMOLECULAR BIOSCIENCE
(2012)
Article
Oncology
Gauri J. Sabnis, Olga G. Goloubeva, Armina A. Kazi, Preeti Shah, Angela H. Brodie
MOLECULAR CANCER THERAPEUTICS
(2013)
Article
Oncology
Armina A. Kazi, Rabia A. Gilani, Amanda J. Schech, Saranya Chumsri, Gauri Sabnis, Preeti Shah, Olga Goloubeva, Shari Kronsberg, Angela H. Brodie
BREAST CANCER RESEARCH
(2014)