Article
Immunology
Gabor J. Szebeni, Robert Alfoeldi, Lajos I. Nagy, Patricia Neuperger, Nikolett Gemes, Jozsef a. Balog, Laszlo Tiszlavicz, Laszlo G. Puskas
Summary: The advent of immunotherapy has revolutionized cancer treatments, but immune checkpoint inhibitors may have severe side effects and therapeutic resistance. A whole-cell tumor vaccine protocol based on ultraviolet C (UCV)-irradiated 4T1 triple-negative breast cancer cells was established, showing partial remission in immunized mice. This approach offers a simple and cost-effective whole-cell vaccine protocol that can be used for immune cell therapy in cancer patients.
Article
Biochemistry & Molecular Biology
Casey D. D. Stefanski, Anne Arnason, Sara Maloney, Janna Kotsen, Elizabeth Powers, Jian-Ting Zhang, Jenifer R. R. Prosperi
Summary: Chemoresistance is a major concern for cancer patients, and one mechanism is the increased expression of ABC transporters, which prevent drug accumulation in cells. Our lab found that the loss of APC gene leads to intrinsic resistance to the drug doxorubicin, potentially through enhanced tumor-initiating cell population and increased STAT3 activation. In mouse mammary tumor cells, the loss of APC decreased drug accumulation and increased protein levels of MDR1 and MRP1. However, the correlation between APC and ABC transporters was not significant in breast cancer patients or cell lines. Inhibitors of ABC transporters showed potential as therapeutic targets in APC-deficient tumors by reducing tumor-initiating cell population and increasing drug-induced apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nanoscience & Nanotechnology
Zhengyue Kang, Min Yang, Xiaoling Feng, Hongjian Liao, Zhifei Zhang, Yonghong Du
Summary: This study reports the therapeutic efficacy of a multifunctional nanoparticle for triple negative breast cancer. The nanoparticle can enhance tumor targeted imaging and promote focused ultrasound ablation, chemotherapy, and sonodynamic therapy. Experimental results demonstrate that this multi-modal synergistic therapy has a higher anti-tumor effect and improves the therapeutic efficacy and prognosis of TNBC.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Oncology
Siang-Boon Koh, Kenneth Ross, Steven J. Isakoff, Nsan Melkonjan, Lei He, Karina J. Matissek, Andrew Schultz, Erica L. Mayer, Tiffany A. Traina, Lisa A. Carey, Hope S. Rugo, Minetta C. Liu, Vered Stearns, Adam Langenbucher, Srinivas Vinod Saladi, Sridhar Ramaswamy, Michael S. Lawrence, Leif W. Ellisen
Summary: This study identifies high levels of RASAL2 as predictors of chemotherapy response and long-term outcomes in TNBC. RASAL2 rewires MAPK feedback and cross-talk to confer resistance to MEK1/2 and EGFR inhibitors while also indicating sensitivity to these inhibitors.
CLINICAL CANCER RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Zeinab Mazloumi, Ali Rafat, Khadijeh Dizaji Asl, Hojjatollah Nozad Charoudeh
Summary: Triple-negative breast cancer (TNBC) is a highly aggressive subtype of breast cancer that is resistant to conventional treatment. In this study, the researchers investigated the use of telomerase inhibition and natural killer (NK) cells as a novel therapy for breast cancer. The results showed that telomerase inhibition increases NK cell cytotoxicity against breast cancer cells and enhances cell death. This combination therapy has the potential to improve the treatment of breast cancer.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Hana Jin, Hye Jung Kim
Summary: This study isolated cancer stem cells (CSCs) from radiotherapy-resistant triple negative breast cancer cells, finding that they increased tumor invasiveness and suppressed NK cell cytotoxicity by regulating NK cell activation ligands.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Medicine, General & Internal
Lorena Alexandra Lisencu, Eduard-Alexandru Bonci, Alexandru Irimie, Ovidiu Balacescu, Cosmin Lisencu
Summary: This review discusses the role of circulating tumor cells (CTCs) in chemoresistance in metastatic breast cancer patients. It highlights that CTCs with stem cell and mesenchymal features are aggressive subtypes that can survive chemotherapy, leading to chemoresistance and disease progression. Hemodynamic shear stress and dynamic changes among CTCs are also important factors in disease progression.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Cell Biology
Seyedeh Zahra Fotook Kiaei, Alireza Nouralishahi, Mohammad Ghasemirad, Maryam Barkhordar, Sasan Ghaffari, Hadis Kheradjoo, Mahshid Saleh, Saman Mohammadzadehsaliani, Zahra Molaeipour
Summary: Breast cancer is the leading cause of cancer death in women, accounting for nearly 15% of newly diagnosed cases. Metastatic disease affects 30% of breast cancer patients, and current treatments cannot cure it, with an average survival time of around 2 years. Immune cell therapy, particularly natural killer (NK) cells and CAR-NK cells, provides a promising approach for treating breast cancer by targeting cancer stem cells.
IMMUNOLOGY AND CELL BIOLOGY
(2023)
Article
Immunology
Yaya Chu, Julie-An Talano, Lee Ann Baxter-Lowe, James W. Verbsky, Erin Morris, Harshini Mahanti, Janet Ayello, Carolyn Keever-Taylor, Bryon Johnson, Rona S. Weinberg, Qiuhu Shi, Theodore B. Moore, Sandra Fabricatore, Brenda Grossman, Carmella van de Ven, Shalini Shenoy, Mitchell S. Cairo
Summary: Haploidentical transplantation with CD34-enriched cells and peripheral blood mononuclear cell addback results in rapid engraftment, sustained donor chimerism, and broad-based immune reconstitution.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Anna Nasulewicz-Goldeman, Waldemar Goldeman, Diana Papiernik, Marcin Nowak, Ewa Mrowczynska, Joanna Wietrzyk
Summary: WG12399C and WG12592A are two novel aminomethylidene bisphosphonates with significant inhibitory effects on bone resorption, as well as anti-metastatic and anti-tumor activities. The study demonstrates that these compounds can effectively reduce the number and size of lung and bone metastases in breast cancer, possibly through inducing apoptosis, inhibiting proliferation, and reducing invasiveness of cancer cells. Therefore, they may have important implications for combined treatment in breast cancer therapy.
Article
Chemistry, Multidisciplinary
Yingzi Ren, Huaqing Jing, Yue Zhou, Chuchu Ren, Guangxu Xiao, Siyu Wang, Xiaoyang Liang, Yunsheng Dou, Ziqiao Ding, Yan Zhu, Xinxing Wang, Nan Li
Summary: Glycolysis inhibition is effective in blocking energy supply and disrupting tumorigenesis in various cancers. However, the breakdown of glutamine as a raw material by tumor cells allows the replenishment pathway to maintain energy supply, resulting in ineffective metabolic interruption. This study presents a comprehensive inhibition of tumor energy supply through the synergistic mediation of glycolysis and the glutamine cycle using iridium oxide nanoparticles loaded with a glutamine transporter antagonist.
CHINESE CHEMICAL LETTERS
(2023)
Article
Pharmacology & Pharmacy
Ammar Said Suliman, Mouhamad Khoder, Ibrahim Tolaymat, Matt Webster, Raid G. Alany, Weiguang Wang, Abdelbary Elhissi, Mohammad Najlah
Summary: In this study, inclusion complexes of DDC-Cu with cyclodextrins were prepared to enhance the solubility of DDC-Cu, showing a significant improvement in solubility and promising anticancer activity against chemoresistant triple negative breast cancer cells.
Article
Medicine, Research & Experimental
Jianhua Pan, Meng Ma, Li Qin, Zhongkui Kang, Dickson Adah, Zhu Tao, Xiaofen Li, Linglin Dai, Siting Zhao, Xiaoping Chen, Qin Zhou
Summary: The study found that Plasmodium infection inhibited tumor growth and increased survival rate in a murine triple negative breast cancer model by enhancing CD8+ T cell responses. This suggests a potential novel strategy for the treatment of triple negative breast cancer through induction of antitumor immune responses mediated by CD8+ T cells.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Yuanyuan He, Somayeh Rezaei, Raimundo Fernandes de Araujo Junior, Luis J. Cruz, Christina Eich
Summary: This study investigated the anti-tumor effects of natural lipids on breast cancer cells. It was found that all tested lipids reduced cell proliferation, migration and invasiveness, thus reducing the malignancy of breast cancer cells. Palmitic acid showed superior anti-tumor properties by inducing apoptosis in cancer cells without affecting healthy cells. Additionally, palmitic acid and ceramide were found to reduce the immunosuppressive phenotype of M2 macrophages and inhibit their promotion of epithelial-mesenchymal transition and migration of breast cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Kuebra Kaban, Clemens Hinterleitner, Yanjun Zhou, Emine Salva, Ayse Gulten Kantarci, Helmut R. Salih, Melanie Maerklin
Summary: The overexpression of antiapoptotic protein BCL-2 is linked with ER expression in breast cancer, and a novel treatment strategy using NK cell-derived exosomes as carriers for BCL-2 targeted siRNAs has been proposed. This approach has shown to enhance the killing ability of NK cell-derived exosomes by silencing BCL-2 overexpression and targeting oncogenes in breast cancer.
Article
Obstetrics & Gynecology
Denise H. J. Habets, Veronique M. M. M. Schiffer, Lisa P. A. Kraneburg, Femke J. W. de Krom, Irem Gurtekin, Bo E. van Bree, Ron J. T. van Golde, Lotte Wieten, Marc E. A. Spaanderman, Salwan Al-Nasiry
Summary: This study aimed to investigate the impact of vascular and metabolic status on recurrent pregnancy loss (RPL). The results showed that the presence of abnormal circulatory factors and constituents of the metabolic syndrome may be associated with RPL.
BMC PREGNANCY AND CHILDBIRTH
(2022)
Letter
Medical Laboratory Technology
Lotte Wieten, Jan G. M. C. Damoiseaux, Bram Lestrade, Liesbeth E. Bakker-Jonges
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Letter
Hematology
Annalisa Ruggeri, Liesbeth C. de Wreede, Carlheinz R. Muller, Pietro Crivello, Edouard F. Bonneville, Effie W. Petersdorf, Gerard Socie, Valerie Dubois, Riitta Niittyvuopio, Juha Perasaari, Ibrahim Yakoub-Agha, Jan J. Cornelissen, Lotte Wieten, Tobias Gedde-Dahl, Edouard Forcade, Charles R. Crawley, Steven G. E. Marsh, Virginie Gandemer, Eleni Tholouli, Claude-Eric Bulabois, Anne Huynh, Goda Choi, Eric Deconinck, Maija Itala-Remes, Stig Lenhoff, Mats Bengtsson, Jan-Erik Johansson, Gwendolyn van Gorkom, Jorinde D. Hoogenboom, Luca Vago, Vanderson Rocha, Chiara Bonini, Christian Chabannon, Katharina Fleischhauer
Article
Cell Biology
Femke A. Ehlers, Timo Olieslagers, Mathijs Groeneweg, Gerard M. J. Bos, Marcel G. J. Tilanus, Christina E. M. Voorter, Lotte Wieten
Summary: The polymorphisms within HLA-C alleles influence the alternative splicing of HLA-C. Skipping variants of exon 5 were observed in HLA-C*04 and *16, which were co-expressed with the mature transcript. The exon 5 SNP specific to HLA-C*16 altered exonic splicing regulatory elements.
Review
Allergy
Denise H. J. Habets, Kim Pelzner, Lotte Wieten, Marc E. A. Spaanderman, Eduardo Villamor, Salwan Al-Nasiry
Summary: IVIG treatment may improve live birth rate in women with recurrent pregnancy loss and underlying immune conditions, especially in subgroups with elevated NK cell percentage and when intervention starts prior to or during conception cycle. However, caution is needed in interpreting these results due to limitations in study design and small sample sizes. Future randomized controlled trials are necessary before widespread clinical use of IVIG.
ALLERGY ASTHMA AND CLINICAL IMMUNOLOGY
(2022)
Article
Medicine, General & Internal
Linda Stevens Brentjens, Denise Habets, Janneke Den Hartog, Salwan Al-Nasiry, Lotte Wieten, Servaas Morre, Aafke Van Montfoort, Andrea Romano, Ron van Golde
Summary: This study aims to investigate the differences in endometrial receptivity parameters between women aged 18-38 with reproductive failure (RIF and RM) and fertile controls, focusing on steroid metabolism, uNK cells, and the microbiome.
Letter
Hematology
Lara S. Boerenkamp, Cilia R. Pothast, Romy C. Dijkland, Kayleigh van Dijk, Gwendolyn N. Y. van Gorkom, Inge H. M. van Loo, Lotte Wieten, Constantijn J. M. Halkes, Mirjam H. M. Heemskerk, Catharina H. M. J. Van Elssen
AMERICAN JOURNAL OF HEMATOLOGY
(2022)
Article
Immunology
Denise H. J. Habets, Anna Schlutter, Sander M. J. van Kuijk, Marc E. A. Spaanderman, Salwan Al-Nasiry, Lotte Wieten
Summary: Our study identified LILRB1 and TACTILE as NK cell receptors associated with RPL. Moreover, we provide first support for the potential role of CMV in RPL via its impact on the NK cell compartment.
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2022)
Review
Oncology
Nicky A. Beelen, Femke A. Ehlers, Gerard M. J. Bos, Lotte Wieten
Summary: This review provides an overview of the impact of inhibitory KIR and NKG2A on NK cell-mediated ADCC response, emphasizing the rationale for combination strategies targeting inhibitory immune checkpoints and ADCC triggering antibodies.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2023)
Review
Oncology
Joel E. J. Beaumont, Nicky A. Beelen, Lotte Wieten, Kasper M. A. Rouschop
Summary: Hypoxia, a characteristic of many cancer types, can suppress the antitumor functions of the adaptive and innate immune system. Tumor-cell-derived extracellular vesicles (EVs), which participate in the communication between tumor cells and immune cells, are also affected by hypoxia and may contribute to immunosuppression. This review summarizes the current understanding of hypoxic cancer-cell-derived EVs in immunosuppression and provides an overview of the factors (miRNA and proteins) enriched in hypoxic tumor-derived EVs and their role in immunomodulation.
Article
Oncology
Femke A. I. Ehlers, Niken M. Mahaweni, Annet van de Waterweg Berends, Thara Saya, Gerard M. J. Bos, Lotte Wieten
Summary: Multiple myeloma (MM) is an incurable disease characterized by malignant plasma cells in the bone marrow. The tumor microenvironment (TME) plays a crucial role in MM growth and is composed of various tumor-associated cells (TAC) that promote immunosuppression. This study explores the interaction between natural killer (NK) cells and TAC, namely tumor-associated macrophages (TAMs) and M1 macrophages, and investigates the potential enhancement of NK cell anti-tumor functions through an ADCC-triggering antibody targeting macrophages.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2023)
Article
Immunology
Leanne P. M. H. van Leeuwen, Marloes Grobben, Corine GeurtsvanKessel, Pauline Ellerbroek, Godelieve de Bree, Judith Potjewijd, Abraham H. Rutgers, Hetty Jolink, Frank van de Veerdonk, Marit L. van Gils, Rory de Vries, Virgil A. S. H. Dalm, VACOPID Res Grp
Summary: This study investigated the immune responses of 473 patients with inborn errors of immunity (IEI) 6 months after receiving mRNA-1273 COVID-19 vaccines. The results showed that antibody titers and T cell responses in IEI patients also declined after 6 months of vaccination, and were more likely to drop below normal levels compared to healthy individuals. Additionally, the effectiveness of a third mRNA COVID-19 vaccine was limited in patients with common variable immunodeficiency (CVID) who did not seroconvert after previous vaccinations.
JOURNAL OF CLINICAL IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Denise H. J. Habets, Salwan Al-Nasiry, Sietse Q. Nagelkerke, Christina E. M. Voorter, Marc E. A. Spaanderman, Taco W. Kuijpers, Lotte Wieten
Summary: This study found that the p.Val176Phe polymorphism in the FCGR3A gene is associated with recurrent pregnancy loss. Patients with at least one p.176Val variant showed increased expression of CD16a receptor and anti-HLA antibodies. However, there was no association between FCGR3A-p.176 polymorphism and the presence of class I and class II anti-HLA antibodies.
SCIENTIFIC REPORTS
(2023)
Article
Nutrition & Dietetics
Lara S. Boerenkamp, Birgit L. M. G. Gijsbers, Erik-Jan Ververs, Eva M. S. Pijpers, Bart Spaetgens, Aniek de Coninck, Wilfred T. V. Germeraad, Will K. W. H. Wodzig, Lotte Wieten, Gwendolyn N. Y. van Gorkom, Catharina H. M. J. van Elssen
Summary: This study investigated the levels of serum and intracellular vitamin C in hospitalized COVID-19 patients and found lower serum vitamin C levels in severe and critical cases. The study suggests that intracellular vitamin C levels decrease during COVID-19 infection, while serum levels remain relatively stable. These findings highlight the importance of further research on the role and mechanisms of intracellular vitamin C in COVID-19 infection.
Article
Immunology
Nicky A. Beelen, Merel R. Aberle, Virginia Bruno, Steven W. M. Olde Damink, Gerard M. J. Bos, Sander S. Rensen, Lotte Wieten
Summary: This study evaluated the cytotoxic potential of adoptive NK cells against pancreatic cancer organoids and found that ADCC-inducing antibodies can enhance NK cell anti-tumor responses.
FRONTIERS IN IMMUNOLOGY
(2023)