Article
Immunology
Honghua Zhang, Xiuzhen Sheng, Xiaoqian Tang, Jing Xing, Heng Chi, Wenbin Zhan
Summary: Lymphocystis disease is a prevalent and transmissible disease in various teleost species. In this study, lymphocystis disease virus (LCDV) infection was detected in naturally infected flounder, and the transcriptome sequencing of skin nodule tissue was performed to understand the molecular mechanism of lymphocystis formation. The transcriptome data revealed the activation of multiple signaling pathways and the regulation of cell cycle-related genes, as well as the expression patterns of genes associated with apoptosis and tumor formation. These findings provide new insights into the molecular mechanisms of lymphocystis formation in fish.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xiuzhen Sheng, Jing Zeng, Ying Zhong, Xiaoqian Tang, Jing Xing, Heng Chi, Wenbin Zhan
Summary: This study confirmed the role of B-lymphocytes in LCDV infection and revealed that LCDV can infect IgM(+) B-cells through a receptor-mediated mechanism and support viral replication.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Yucong Yang, Yuqin Ren, Yitong Zhang, Guixing Wang, Zhongwei He, Yufeng Liu, Wei Cao, Yufen Wang, Songlin Chen, Yuanshuai Fu, Jilun Hou
Summary: A new cell line, Japanese flounder spleen (JFSP), derived from the spleen of Japanese flounder (Paralichthys olivaceus), was established and characterized in this study. The JFSP cells grew rapidly at 29 degrees C, and the optimum fetal bovine serum concentration in the L-15 medium was 15%. Cells were subcultured for more than 80 passages. The JFSP cells have a diploid chromosome number of 2n = 68, which differs from the chromosome number of normal diploid Japanese flounder. The established cells were susceptible to Bohle virus (BIV), Viral hemorrhagic septicemia virus (VHSV), Hirame rhabdovirus (HIRRV), Infectious hematopoietic necrosis virus (IHNV), and Lymphocystis disease virus (LCDV), as evidenced by varying degrees of cytopathic effects (CPE). Replication of the virus in JFSP cells was confirmed by qRT-PCR and transmission electron microscopy. In addition, the expression of four immune-related genes, TRAF3, IL-1 beta, TNF-alpha, and TLR2, was differentially altered following viral infection. The results indicated that the cells underwent an antiviral immune response. JFSP cell line is an ideal tool in vitro for virology. The use of fish cell lines to study the immune genes and immune mechanism of fish and to clarify the immune mechanism of fish has important theoretical significance and practical application value for the fundamental prevention and treatment of fish diseases.
Article
Agriculture, Dairy & Animal Science
Ming-Chung Cheng, Ming She See, Pei-Chi Wang, Yu-Ting Kuo, Yuan-Shing Ho, Shih-Chu Chen, Ming-An Tsai
Summary: This research focuses on the infection of Lymphocystic Disease Virus (LCDV) in Amphiprion ocellaris and Amphiprion clarkii. LCDV was detected through histopathological study, observation of virus particles under an electron microscope, and gene sequence analysis. Symptoms of LCDV included white, stiff nodules on the body, skin, gills, and fins. The study found that the genotype of LCDV was closely related to a strain of LCDV from paradise fish in China.
Article
Fisheries
Yucong Yang, Yitong Zhang, Yuqin Ren, Zhongwei He, Wei Cao, Yufeng Liu, Jiangong Ren, Yufen Wang, Guixing Wang, Yuanshuai Fu, Jilun Hou
Summary: Slc2a6 is involved in the early embryonic development and inflammatory response of Japanese flounder, and its expression is regulated by the NF-kappa B signaling pathway. After stimulation by lymphocystis disease virus (LCDV), Slc2a6 expression is increased and it is involved in viral regulation by regulating innate immune responses in Japanese flounder.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Virology
Jessica Benkaroun, Sven M. Bergmann, Angela Roemer-Oberdoerfer, Menekse Didem Demircan, Cuneyt Tamer, Gayatri Rajendra Kachh, Manfred Weidmann
Summary: Lymphocystis disease viruses (LCDVs) are found in various locations worldwide and infect bony fish. The genome size of LCDVs varies, with the LCDV1 genome being approximately half the size of LCDV2-4 genomes. Whole genome sequencing and analysis of LCDVs from wild fish and aquafarmed sea bream confirm this difference in genome size and suggest an adaptation to the lifestyle of demersal fish in the northeast Atlantic.
Article
Agriculture, Dairy & Animal Science
Mohamed Shawky, Engy Taha, Basem Ahmed, Mahmoud Aly Mahmoud, Mohamed Abdelaziz, Mohamed Faisal, Ausama Yousif
Summary: This study investigated the outbreak of lymphocystis disease in juvenile gilthead seabreams imported to Egypt from Europe, identifying the causative agent as lymphocystis disease virus genotype I, with genetic similarity to strains found in Northern European countries. These findings contribute to the understanding of the ecology of lymphocystis disease and its viral genotypes in the region.
Article
Microbiology
Satoshi Kawato, Reiko Nozaki, Ikuo Hirono, Hidehiro Kondo
Summary: The genome sequence of lymphocystis disease virus 2 LCDV-JP_Oita_2018, isolated from a diseased Japanese flounder in Japan, is presented here. The circular contig of LCDV-JP_Oita_2018 genome is 186,627 bp long, with 140 predicted protein-coding genes and a GC content of 27%.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Chemistry, Multidisciplinary
Benjamin B. Tournier, Farha Bouteldja, Quentin Amossee, Alekos Nicolaides, Marcelo Duarte Azevedo, Liliane Tenenbaum, Valentina Garibotto, Kelly Ceyzeriat, Philippe Millet
Summary: An increase in astrocyte reactivity in Alzheimer's disease is related to a pro-inflammatory environment. TSPO, a protein involved in astrocyte functions, has been found to increase under inflammatory conditions, but its mechanisms are not well understood. This study aimed to understand the mechanisms behind the increase in TSPO and its consequences. The results showed that LPS induced TSPO expression and increased mitochondrial density in astrocytes through a STAT3-dependent mechanism. LPS also stimulated ROS production and decreased glucose consumption, effects that were attenuated by a TSPO antagonist. Overexpression of TSPO increased ROS production, and the combination of TSPO overexpression and LPS had a synergistic effect on ROS production. These findings suggest that TSPO plays a role in glucose metabolism regulation and pro-inflammatory response in astrocytes, potentially contributing to the harmful effects in Alzheimer's disease.
Article
Immunology
Yu Yan, Cheng Lingling, Xu Zhiyong, Zhang Yanhong, Ou Changbo, Wang Qiuxia, Gao Pei, Ma Jinyou
Summary: The study investigated the tissue distribution, developmental changes, and response to IBDV infection of IRFs in chickens using qRT-PCR. Results showed tissue-specific expression of different chIRFs and their close association with the development and immune response of BF. Infection with IBDV led to activation or suppression of certain chIRFs, which may provide insights for antiviral gene selection and vaccine adjuvant development.
MICROBIAL PATHOGENESIS
(2021)
Article
Infectious Diseases
Saul Jimenez-Ruiz, Joaquin Vicente, Ignacio Garcia-Bocanegra, Oscar Cabezon, Maria Cruz Arnal, Ana Balseiro, Francisco Ruiz-Fons, Felix Gomez-Guillamon, Sonia Lazaro, Fernando Escribano, Pelayo Acevedo, Lucas Dominguez, Christian Gortazar, Daniel Fernandez de Luco, Maria A. Risalde
Summary: A large-scale study in Spain found prevalence of antibodies against Pestivirus in wild ruminants, with higher rates in Southern chamois. Limited circulation of viruses in non-chamois species suggests they are not true Pestivirus reservoirs. Surveillance of Pestivirus in wild ruminants is crucial in preventing new outbreaks and transmission to livestock.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Fisheries
Hooman Rahmati-Holasoo, Arash Ghalyanchilangeroudi, Zahra Ziafati Kafi, Amin Marandi, Sara Shokrpoor, Bahram Imantalab, Hosseinali Ebrahimzadeh Mousavi
Summary: This study reports the first detection and characterization of Lymphocystis disease virus (LCDV) in yellowbar angelfish (Pomacanthus maculosus) in Iran. Clinical inspection and histopathological analysis of infected fish revealed increased mortality, whitish nodules on the fins and skin, hypertrophic lymphocystis cells, and other pathological changes.
Article
Ecology
Nathan C. Layman, Andrew J. Basinski, Boyu Zhang, Evan A. Eskew, Brian H. Bird, Bruno M. Ghersi, James Bangura, Elisabeth Fichet-Calvet, Christopher H. Remien, Mohamed Vandi, Mohamed Bah, Scott L. Nuismer
Summary: This study develops a method that learns the spatial distribution of a reservoir species from aerial imagery, using neural networks to extract features and linking them to reservoir presence/absence data to accurately evaluate the likelihood of reservoir occurrence.
Article
Immunology
Yanbo Hao, Xiaoqian Tang, Jing Xing, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: The Fc receptor plays a critical role in mediating the phagocytosis and bactericidal activity of IgM(+) B lymphocytes in flounder. It increases phagocytosis rates and affects intracellular reactive oxygen species levels. Additionally, blocking Fc receptor or Fc fragment of IgM reduces phagocytosis rates in mIgM(+) B lymphocytes.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Laurie Spencer, Babatunde Olawuni, Pallavi Singh
Summary: The study of the intestinal microbiome is progressing, with increasing recognition of the importance of the gut virome in human health and disease. Viruses, particularly bacteriophages, have significant biochemical and genetic activities in the complex intestinal environment, and are associated with gastrointestinal diseases. Further research on the gut virome is crucial for understanding the role of the intestinal microbiome in human health and disease.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Pathology
Teruaki Hirota, Kayoko Yonemaru, Mitsunobu Hattori, Mami Murakami, Hiroki Sakai, Akihiro Hirata
Summary: An 11-year-old female Persian cat underwent ovariohysterectomy due to dilation of the uterine cavity with irregular thickening of the wall. The pathological examination revealed multiple nodules originating from the endometrium, invading the myometrium and subserosa, and immunohistochemical staining confirmed the diagnosis of endometrial stromal sarcoma.
JOURNAL OF COMPARATIVE PATHOLOGY
(2024)
