期刊
ANALYTICAL CHEMISTRY
卷 87, 期 19, 页码 9939-9945出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.5b02517
关键词
-
资金
- Federal Ministry of Education and Research (BMBF
- e:Bio project ReelinSys)
- Young Investigator Program of the Research Center of Infectious Diseases (ZINF) of the University of Wuerzburg, Germany
- German Research Foundation DFG [SI 1610/2-1]
- Human Frontier Science Program
We introduce fragment ion patchwork quantification as a new mass spectrometry-based approach for the highly accurate quantification of site-specific acetylation degrees. This method combines C-13(1)-acetyl derivatization on the protein level, proteolysis by low-specificity proteases and quantification on the fragment ion level. Acetylation degrees are determined from the isotope patterns of acetylated b and y ions. We show that this approach allows to determine site-specific acetylation degrees of all lysine residues for all core histones of Trypanosoma brucei. In addition, we demonstrate how this approach can be used to identify substrate sites of histone acetyltransferases.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据