期刊
BRAZILIAN JOURNAL OF MICROBIOLOGY
卷 45, 期 1, 页码 333-341出版社
SPRINGER
DOI: 10.1590/S1517-83822014005000032
关键词
antimycobacterial avticity; lupulone; DNA microarray
类别
资金
- Fund for National Nature Science Foundation of China [31000822, 31271951, 31172364]
- Specialized Research Fund for Important National Science & Technology Specific Projects [2012ZX10003002]
- Program for New Century Excellent Talents in University [NCET-09-0434, NCET-13-0245]
- China Postdoctoral Science Foundation [2013M530142]
- Shenzhen biological special funds for industrial development aid key basic research project [JC201005280643A]
Mycobacterium tuberculosis (M. tuberculosis), the causative agent of tuberculosis, still causes higher mortality than any other bacterial pathogen until now. With the emergence and spread of multidrug-resistant (MDR) and extensively drug-resistant (XDR-TB) strains, it becomes more important to search for alternative targets to develop new antimycobacterial drugs. Lupulone is a compound extracted from Hops (Hurnulus lupulus), which exhibits a good antimicrobial activity against M. tuberculosis with minimal inhibitory concentration (MIC) value of 10 mu g/mL, but the response mechanisms of lupulone against M. tuberculosis are still poorly understood. In this study, we used a commercial oligonucleotide microarray to determine the overall transcriptional response of M. tuberculosis H37Rv triggered by exposure to MIC of lupulone. A total of 540 genes were found to be differentially regulated by lupulone. Of these, 254 genes were upregulated, and 286 genes were downregulated. A number of important genes were significantly regulated which are involved in various pathways, such as surface-exposed lipids, cytochrome P450 enzymes, PE/PPE multigene families, ABC transporters, and protein synthesis. Real-time quantitative RT-PCR was performed for choosed genes to verified the microarray results. To our knowledge, this genome-wide transcriptomics approach has produced the first insights into the response of M. tuberculosis to a lupulone challenge.
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