Article
Agriculture, Dairy & Animal Science
Shamsaldeen Ibrahim Saeed, Khairun Anisa Mat Yazid, Hidayatul Athirah Hashimy, Siti Khadijah Dzulkifli, Fatihah Nordin, Nik Azmi Nik Him, Mohd Fikry Fahmi bin Omar, Erkihun Aklilu, Maizan Mohamad, Che Wan Salma Zalati, Nor Fadhilah Kamaruzzaman
Summary: This study reports the prevalence and characterization of Staphylococcus aureus (S. aureus) isolated from bovine mastitis in dairy farms in East Coast Peninsular Malaysia. The study shows the presence of subclinical mastitis and antimicrobial resistance (AMR) in S. aureus isolated from milk samples. Furthermore, S. aureus isolates demonstrate the ability to invade mammary epithelial cells and form biofilms, presenting challenges to mastitis treatment.
Article
Veterinary Sciences
Eman E. Abdeen, Walid S. Mousa, Ashraf A. Abdel-Tawab, Rasha El-Faramawy, Usama H. Abo-Shama
Summary: This study focused on the phenotypic, genotypic, and antibiogram characterization of Staphylococcus aureus from bovine subclinical mastitis in Menoufiya Governorate, Egypt. Results showed a high prevalence of MRSA strains with multidrug resistance, and the presence of antibiotic resistance and virulence genes in the S. aureus strains. Regular surveillance of phenotypic and genotypic profiles of S. aureus isolates is crucial for effective control measures and to prevent the evolution of multidrug-resistant strains.
PAKISTAN VETERINARY JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Ramamoorthy Sivakumar, Parameswaran Sree Pranav, Madhavi Annamanedi, S. Chandrapriya, Shrikrishna Isloor, Jeyaprakash Rajendhran, Nagendra R. R. Hegde
Summary: This study conducted a comprehensive analysis of bovine mastitis-associated S. aureus isolates from India using whole genome sequencing and comparative genomic analysis. The results revealed 15 different sequence types and 5 clonal complexes. The genome of Indian mastitis-associated S. aureus strains is relatively closed. Phylogenetic analysis based on SNP variation differentiated the 41 strains into six major clades. Seventeen different antimicrobial resistance genes and 108 virulence-associated genes were identified among these isolates.
Article
Veterinary Sciences
Nikolina Rusenova, Nasko Vasilev, Anton Rusenov, Aneliya Milanova, Ivo Sirakov
Summary: The study aimed to assess the resistance of bovine mastitis S. aureus isolates in Bulgaria to different classes of chemotherapeutic drugs through comparison of phenotypic and genotypic methods. High levels of resistance to sulfadimethoxine were found, along with resistance to other antibiotics. A comprehensive evaluation through standard and modern molecular methods is recommended to address discrepancies in resistance patterns.
VETERINARY SCIENCES
(2022)
Article
Immunology
Hussain A. Alabdullah, Elise Overgaard, Danielle Scarbrough, Janet E. Williams, Omid Mohammad Mousa, Gary Dunn, Laura Bond, Mark A. McGuire, Juliette K. Tinker
Summary: The study assessed the efficacy of a vaccine against S. aureus infection, showing vaccinated animals did not exhibit clinical symptoms, had enhanced immune responses, and potential protective effects.
Review
Veterinary Sciences
Ana Ines Molineri, Cecilia Camussone, M. Virginia Zbrun, Guillermo Suarez Archilla, Mariana Cristiani, Veronica Neder, Luis Calvinho, Marcelo Signorini
Summary: Staphylococcus aureus shows increasing antimicrobial resistance over time, with penicillin, clindamycin, erythromycin, and gentamycin having the highest resistance rates. Africa, Asia, and Latin America display higher resistance rates, with no significant differences in resistance based on the clinical origin of the isolates.
PREVENTIVE VETERINARY MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Changjie Lin, Yifan Zhu, Zhiyu Hao, Haojun Xu, Ting Li, Jinghan Yang, Xi Chen, Yingyu Chen, Aizhen Guo, Changmin Hu
Summary: This study investigated the effects of E.coli and S.aureus on bovine mammary epithelial cell injuries and explored the lncRNA profile. The results showed differential expression of a large number of lncRNAs induced by these two pathogens, targeting signaling pathways with similar functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Veterinary Sciences
Kuan Wang, Jinlong Cha, Kai Liu, Jianming Deng, Bowen Yang, Hui Xu, Juyu Wang, Limei Zhang, Xiaolong Gu, Cuiqin Huang, Weijie Qu
Summary: A meta-analysis was conducted to investigate the epidemiology and antimicrobial resistance of Staphylococcus aureus-associated bovine mastitis in China. The study found that the prevalence of S. aureus has decreased over time, but is still higher in China compared to Western countries. The antimicrobial resistance analysis showed high resistance to beta-lactam antibiotics.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Review
Veterinary Sciences
Bruno Campos, Amy C. Pickering, Lis Souza Rocha, Ananda Pereira Aguilar, Mary Hellen Fabres-Klein, Tiago Antonio de Oliveira Mendes, J. Ross Fitzgerald, Andrea de Oliveira Barros Ribon
Summary: This review summarizes the research on bovine Staphylococcus aureus population genetics and molecular pathogenesis conducted worldwide in the past 16 years. It describes the diversity and geographical distribution of S. aureus associated with bovine mastitis, and explores the evolution of bovine S. aureus and the importance of host-adaptation in its emergence as a mastitis pathogen. The functional relevance of virulence determinants in the pathogenesis of bovine mastitis is also discussed.
BMC VETERINARY RESEARCH
(2022)
Article
Microbiology
Ryota Miyazawa, So Shimoda, Keiichi Matsuda, Ryuta Tobe, Tasuke Ando, Hiroshi Yoneyama
Summary: Staphylococcus aureus is a major pathogen in humans and livestock, causing serious issues such as bovine mastitis. This study monitored cases of intramammary infection caused by S. aureus in the Miyagi Prefecture, Japan, and analyzed the strains obtained from dairy farms. The study found recurrent cases of infection and identified a Methicillin-Sensitive SA (MSSA) ST398 strain isolated from mastitis-containing cows, which is the first confirmed case in Japan. The results highlight the importance of nationwide surveillance to manage the distribution of ST398 strains in dairy farms for human and animal health.
Review
Veterinary Sciences
Aram Sharifi, Keyvan Sobhani, Peyman Mahmoudi
Summary: Staphylococcus aureus (S. aureus) is a major cause of bovine mastitis and exhibits high levels of antimicrobial resistance (AMR), particularly against penicillin G and ampicillin. A systematic review of published data from Iran between 2000 and 2021 revealed a high prevalence of AMR among Iranian isolates of S. aureus causing bovine mastitis. The increasing trend of AMR in recent years highlights the need for strengthened control strategies to prevent the spread of this pathogen and drug resistance.
RESEARCH IN VETERINARY SCIENCE
(2023)
Article
Microbiology
Amira A. Moawad, Hosny El-Adawy, Joerg Linde, Ines Jost, Gaertner Tanja, Hruschka Katja, Donat Karsten, Heinrich Neubauer, Stefan Monecke, Herbert Tomaso
Summary: This study investigated the genetic diversity, antimicrobial resistance profiles, and virulence-associated factors of Staphylococcus aureus isolated from clinical bovine mastitis in Thuringia, Germany. The results showed that different sequence types and clusters of S. aureus were widely distributed among dairy farms in Thuringia, with the potential for large-scale spread.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Jabin Nesaraj, Alex Grinberg, Richard Laven, Patrick Biggs
Summary: We analyzed the genomes of 188 bovine-mastitis-causing S. aureus isolates from 65 dairy farms in New Zealand over a 17-year period. The analysis revealed a dominant clonal complex (CC1/ST1) that accounted for 75% of the isolates. This clonal complex showed host adaptation to cattle and exhibited temporal clonal stability.
VETERINARY MICROBIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Minhye Shin, Daye Mun, Hye Jin Choi, Sooah Kim, Shelley M. Payne, Younghoon Kim
Summary: Staphylococcus aureus RF122 Feo system is a major pathogen causing bovine mastitis, and the new antimicrobial agent PHT-427 efficiently inhibits this system, attenuating various virulence factors related to milk quality, enhancing bacterial antibiotic susceptibility, and showing no toxicity on animal model systems.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Veterinary Sciences
Junjun Liu, Xin Wang, Chongliang Bi, Khalid Mehmood, Farah Ali, Jianhua Qin, Zhaoqing Han
Summary: This study investigated Staphylococcus aureus in mastitis bovine milk in Anhui and found its drug resistance and virulence genes. Understanding the drug resistance situation of S. aureus is of great importance for the prevention and treatment of public health concerns in this region.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Veterinary Sciences
T. G. Rocha, F. D. F. Silva, C. Bortoletto, D. G. Silva, M. G. Buzinaro, L. F. Zafalon, J. J. Fagliari
ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA
(2016)
Article
Agronomy
Luiz Francisco Zafalon, Guilherme Aparecido Fim Junior, Carolina Orlando Vaso, Nahryda Samara dos Santos Lopes, Josir Laine Aparecida Veschi, Raul Costa Mascarenhas Santana
Article
Veterinary Sciences
Raul C. M. Santana, Luiz Francisco Zafalon, Humberto de M. Brandao, A. F. Junior Guilherme, Lucas Eduardo Pilon, B. Junior Waldomiro, Rodrigo Gigliotie, Vanessa Carla F. Mosqueira
PESQUISA VETERINARIA BRASILEIRA
(2016)
Article
Agriculture, Dairy & Animal Science
Luiz Francisco Zafalon, Raul Costa Mascarenhas Santana, Lucas Eduardo Pilon, Guilherme Aparecido Fim Junior
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2016)
Article
Agriculture, Dairy & Animal Science
Katheryne B. Martins, Patricia Y. Faccioli, Mariana F. Bonesso, Simone Fernandes, Aline A. Oliveira, Ariane Dantas, Luiz F. Zafalon, Maria de Lourdes R. S. Cunha
JOURNAL OF DAIRY SCIENCE
(2017)
Article
Microbiology
Lucas J. L. Pizauro, Camila C. de Almeida, Glenn A. Soltes, Durda Slavic, Oswaldo D. Rossi-Junior, Fernando. A. de Avila, Luiz. F. Zafalon, Janet I. MacInnes
VETERINARY MICROBIOLOGY
(2017)
Article
Veterinary Sciences
Luiz F. Zafalon, Maria L. R. S. Cunha, Humberto M. Brandao, Vanessa C. F. Mosqueira, Raul C. M. Santana, Waldomiro Barioni Junior, Katheryne B. Martins, Lucas E. Pilon
PESQUISA VETERINARIA BRASILEIRA
(2018)
Article
Veterinary Sciences
Luiz F. Zafalon, Maria L. R. S. Cunha, Humberto M. Brandao, Vanessa C. F. Mosqueira, Raul C. M. Santana, Waldomiro Barioni Junior, Katheryne B. Martins, Lucas E. Pilon
PESQUISA VETERINARIA BRASILEIRA
(2018)
Article
Agriculture, Multidisciplinary
Luiz Francisco Zafalon, Raul Costa Mascarenhas Santana, Sergio Novita Esteves, Guilherme Aparecido Fim Junior
SEMINA-CIENCIAS AGRARIAS
(2018)
Article
Veterinary Sciences
Thais G. Rocha, Camila Bortoletto, Daniela G. Silva, Kalina M. M. G. Simplicio, Luiz F. Zafalon, Jose J. Fagliari
PESQUISA VETERINARIA BRASILEIRA
(2019)
Article
Agriculture, Dairy & Animal Science
Lucas J. L. Pizauro, Camila C. de Almeida, Glenn A. Soltes, Durda Slavic, Fernando A. de Avila, Luiz F. Zafalon, Janet I. MacInnes
JOURNAL OF DAIRY SCIENCE
(2019)
Article
Multidisciplinary Sciences
Lucas Jose Luduverio Pizauro, Camila Chioda de Almeida, Saura Rodrigues Silva, Janet I. MacInnes, Andrew M. Kropinski, Luiz Francisco Zafalon, Fernando Antonio de Avila, Alessandro de Mello Varani
Summary: Mastitis is a common and costly disease on dairy farms caused by Staphylococcus species. Genomic analyses revealed prevalent adhesion, biofilm, and regulatory genes in clinical and subclinical isolates, with potential for virulence profiling or vaccine development. Horizontal dissemination of these genes between clinical and subclinical isolates was suggested by co-phylogenetic analyses.
SCIENTIFIC REPORTS
(2021)
Article
Veterinary Sciences
Elka Machado Ferreira, Leticia Castilho Romero, Maria de Lourdes Ribeiro de Souza da Cunha, Wilson Malago Junior, Carlos Henrique Camargo, Waldomiro Barioni Junior, Luiz Francisco Zafalon
Summary: The study investigated the in vitro production of biofilms and gene distribution of Staphylococcus spp. isolated from cows treated with homeopathy, finding that the production of biofilm was associated with the presence of specific genes, and there were differences in the diversity and dissemination of S. aureus in cows treated and not treated with homeopathy.
BMC VETERINARY RESEARCH
(2022)
Article
Veterinary Sciences
Thais G. Rocha, Ketherson R. Silva, Fernanda D. F. Silva, Amauri A. Alfieri, Daniela G. Silva, Helio Jose Montassier, Maria da Gloria Buzinaro, Fabio Gregori, Luiz Francisco Zafalon, Jose Jurandir Fagliari
PAKISTAN VETERINARY JOURNAL
(2020)
Article
Microbiology
Lucas J. L. Pizauro, Camila C. de Almeida, Iman M. Gohari, Janet I. MacInnes, Luiz Francisco Zafalon, Andrew M. Kropinski, Alessandro M. Varani
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2019)