期刊
BRAZILIAN JOURNAL OF MICROBIOLOGY
卷 40, 期 4, 页码 893-900出版社
SPRINGER
DOI: 10.1590/S1517-83822009000400021
关键词
Profenofos; Organophosphate pesticide; Gas Chromatography; Bioremediation
类别
资金
- National Key Technology R & D Program of China [2008BADA7B03]
- City Key Technology R & D Program of Wuhan in China [200720422150]
An enrichment culture technique was used to isolate bacterial strains responsible for the biodegradation of profenofos in a soil from Hubei province of central China. Two pure bacterial cultures, named W and Y, were isolated and subsequently characterized by sequencing of 16S rRNA genes and biochemical tests. Isolate W showed 96% similarity to the 16S rRNA gene of a Pseudomonas putida unlike Y which showed 99% similarity to the 16S rRNA gene of Burkholderia gladioli. Both strains grew well at pH 5.5-7.2 with a broad temperature profile ranging from 28 degrees to 36 degrees C. Bioremediation of profenofos-contaminated soil was examined using soil treated with 200 ug g(-1); profenofos resulted in a higher degradation rate than control soils without inoculation. In a mineral salt medium (FTW) reduction in profenofos concentration was 90% within 96 hours of incubation. A literature survey revealed that no data is available regarding the role of Burkholderia gladioli on pesticide biodegradation as well as on profenofos.
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