期刊
BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING
卷 28, 期 1, 页码 45-49出版社
BRAZILIAN SOC CHEMICAL ENG
DOI: 10.1590/S0104-66322011000100006
关键词
Spirulina; Wet biomass; Extraction; C-Phycocyanin
资金
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - CAPES
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq
C-Phycocyanin is a natural blue dye used in food and pharmaceutical industry. In the present study, a simple and efficient method to extract C-phycocyanin from Spirtulina platensis wet biomass is reported. The extractions were carried out using six different methods, including chemical (organic and inorganic acid treatment), physical (freezing and thawing, sonication, homogenization) and enzymatic (lysozyme treatment) methods. The extraction using ultrasonic bath in the presence of glass pearls in the biomass proved to be the most efficient method, 56% higher than using freezing and thawing (the method most frequently used), and presented a extraction yield of 43.75 mg.g(-1) and a C-phycocyanin concentration of 0.21 mg.mL(-1).
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