期刊
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
卷 53, 期 3, 页码 643-652出版社
INST TECNOLOGIA PARANA
DOI: 10.1590/S1516-89132010000300020
关键词
Bacillus subtilis; batch cultivation; oxygen availability; antimicrobial activity
类别
资金
- Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
The physiology of B. subtilis R14 was investigated in minimal medium under excess-oxygen and oxygen-limited conditions. Growth and efficient sporulation could be achieved in excess-oxygen culture on medium with readily metabolizable carbon and nitrogen sources, which allowed high growth rate and high biomass yield. A short transition phase between the exponential growth and sporulation could be attained by formulating a medium with a well-balanced C/N ratio. Under oxygen-limitation, but in the presence of essential nutrients (i.e. excess-nutrient cultivation), B. subtilis R14 produced bioactive compounds, which showed activity against several phytopathogenic bacteria. Under anaerobic condition, the organism did not grow neither through fermentation nor anaerobic respiration. However, addition of pyruvate to the medium allowed its growth through fermentation and anaerobic respiration. The knowledge acquired in this work could be relevant both for the design of a production process as well as for the formulation of an effective commercial biocontrol product.
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