期刊
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
卷 52, 期 6, 页码 1349-1356出版社
INST TECNOLOGIA PARANA
DOI: 10.1590/S1516-89132009000600005
关键词
Central composite design; Enterobacter aerogenes; Lipase; optimization; parameter; Response Surface Methodology
类别
资金
- CSIR, Govt. of India
- DBT, India
Optimization of lipase production by Enterobacter aerogenes was carried out using response surface methodology (RSM) where the statistical model was obtained by fractional factorial central composite design. The influence of various physico-chemical parameters, viz. temperature, oil concentration, inoculum volume, pH and incubation period on lipase production was examined. Optimization of physico-chemical parameters resulted 1,4-fold increase in lipase activity. The optimum levels of parameters were 34 degrees C, oil concentration 3%, inoculum volume 7%, pH 7 and incubation time 60 h for obtaining a maximum lipase activity of 27.25 U/ml.
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