期刊
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
卷 51, 期 6, 页码 1233-1240出版社
INST TECNOLOGIA PARANA
DOI: 10.1590/S1516-89132008000600019
关键词
beta-galactosidase; immobilization; lactase; whey; lactose; hydrolysis
类别
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
Hydrolysis of whey lactose to glucose and galactose by immobilized galactosidase comes as an alternative to enlarge the possibilities of commercial use of this feedstock. To be applied at industrial scale, the process should be performed continuously. This work aimed to study the hydrolysis of whey lactose by an immobilized enzyme reactor. beta-Galactosidase from Aspergillus oryzae was immobilized on, silica and activity and stability were evaluated. The best immobilization results were attained by using glutaraldehyde as support's activator and enzyme stabilizer. The optimized enzyme proportion for immobilization was 12-20 mg g-I of support. Treatments of why were performed (microfiltration, thermal treatment and ultrafiltration), seeking the elimination of sludge, and the effects on operating the fixed bed reactor were evaluated. Ultrafiltration was the best treatment towards a proper substrate solution for feeding the reactor.
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