期刊
BRAIN RESEARCH BULLETIN
卷 96, 期 -, 页码 71-85出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.brainresbull.2013.05.004
关键词
P2X(7) receptor; Stellate ganglia; Dorsal root ganglia; Myocardial ischemic injury; Sensory-sympathetic coupling
资金
- National Natural Science Foundation of China [81171184, 31060139, 30860086, 30660048, 81100829, 81260187, 81200853]
- Natural Science Foundation of Jiangxi Province [0640042, 2008GZY0029]
- Technology Pedestal and Society Development Project of Jiangxi Province [2010BSA09500, 20111BBG70009-1]
- Education Department of Jiangxi Province [GJJ1113155]
Purinergic signaling has been found to participate in the regulation of cardiovascular function. In this study, using a rat myocardial ischemic injury model, the sympathoexcitatory reflex mediated by P2X(7) receptor via sensory-sympathetic coupling between cervical dorsal root ganglia (DRG) nerves and stellate ganglia (SG) nerves was explored. Our results showed that the systolic blood pressure, heart rate, serum cardiac enzymes concentrations, interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) concentrations were increased, and the expression levels of P2X(7) mRNA and protein in DRG and SG were up-regulated after myocardial ischemic injury. Administration of brilliant blue G (BBG), a selective P2X(7) antagonist, decreased the elevation of systolic blood pressure, heart rate, serum cardiac enzyme, IL-6 and TNF-alpha, and inhibited the up-regulated expression of P2X(7) mRNA and protein in DRG and SG after myocardial ischemic injury. Retrograde tracing test showed that there were calcitonin gene-related peptide sensory nerves and substance P sensory nerves sprouting from DRG to SG, which played an important role in the development of myocardial ischemic injury. The up-regulated P2X(7) receptor expression levels on the surface membrane of satellite glial cells contributed to the activation of sensory-sympathetic coupling, which in turn facilitated the sympathoexcitatory reflex. BBG can inhibit the activation of satellite glial cells and interrupt the generation of sensory-sympathetic coupling in the cervical sympathetic ganglia after the myocardial ischemic injury. Taken together, these findings may provide a new therapeutic approach for treating coronary heart disease, hypertension and other cardiovascular diseases. (C) 2013 Elsevier Inc. All rights reserved.
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