期刊
BRAIN RESEARCH
卷 1491, 期 -, 页码 188-196出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.brainres.2012.10.046
关键词
Hydrogen sulfide (H2S); Ischemia-reperfusion; Brain; Oxidative stress; Inflammation; Apoptosis
资金
- National Natural Science Foundation of China [30901555]
- Central South University [YC09141]
The present study was undertaken to study the effects of exogenous hydrogen sulfide (H2S) on global cerebral ischemia-reperfusion(I/R) and the underlying mechanisms. After a 24 h I/R, administration of NaHS, an exogenous donor for H2S, at the dose of 0.2 or 0.4 mu mol/kg significantly decreased the apoplexy index, neurological symptom scoring, and brain infarcted area as compared to the I/R group in a dose dependent manner. At the same time, NaHS-treated rats displayed significant reduction of MDA content, and striking increase of SOD activity in the brain tissues compared with I/R group. The up-regulated mRNA levels of p47(phox) and gp91(phox) subunits of NADPH oxidase were also suppressed by NaHS treatment. In this study, the pro-inflammatory markers TNF-alpha and MCP-1 in I/R group were markedly increased by 24 h I/R, which were significantly attenuated by NaHS in a dose-dependent manner. In contrast, the anti-inflammatory factor IL-10 was markedly induced by NaHS administration. In addition, the expression of the anti-apoptotic protein Bcl-2 was significantly decreased in I/R group compared with the sham-operated group. This reduction was significantly blunted in NaHS-treated group. On the contrary, the proapoptotic protein Bax content in brain tissues of I/R group was markedly elevated compared with sham-operated animals. And such an induction of Bax content was significantly ameliorated by NaHS. Taken together, our results suggest that hydrogen sulfide has potent protective effect against a severe cerebral injury induced by a global I/R possibly through the inhibition of oxidative stress, inflammation and apoptosis. (C) 2012 Elsevier B.V. All rights reserved.
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