期刊
BRAIN RESEARCH
卷 1462, 期 -, 页码 162-173出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.brainres.2012.01.068
关键词
RNA-Binding protein (RBP); Musashi; Zebrafish; Neural development
资金
- Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT)
- Keio Gijuku Academic Development Funds
- Strategic Research Foundation Grant-aided Project for Private Universities, MEXT
- Keio Gijuku Fukuzawa Memorial Fund for the Advancement of Education and Research
- Uehara Memorial and Mitsukoshi Health and Welfare Foundations
- Global COE Program of MEXT
- Grants-in-Aid for Scientific Research [22600011] Funding Source: KAKEN
Musashi (Msi) is an evolutionarily conserved gene family of RNA-binding proteins (RBPs) that is preferentially expressed in the nervous system. The first member of the Msi family was identified in Drosophila. Drosophila Msi plays an important role in regulating asymmetric cell division of the sensory organ precursor cells. The mammalian orthologs, including human and mouse Musashi1 (Msi1), are neural RBPs that are strongly expressed in fetal and adult neural stem/progenitor cells (NS/PCs). Mammalian Msi1 contributes to self renewal of NS/PCs through translational regulation of several target mRNAs. In this study, the zebrafish Msi ortholog zMsi1 was identified and characterized. The normal spatial and temporal expression profiles for both protein and mRNA were determined. A series of splice variants were detected. Overall, zMsi1 was strongly expressed in neural tissue in early stages of development and exhibited similarity to mammalian Msi1 expression patterns. To reveal the in vivo function of zMsi1, morpholinos against Msi1 were introduced into one-cell stage zebrafish embryos. Knock down of zmsi1 frequently resulted in aberrant formation of the Central Nervous System (CNS). These results suggest that Msi1 plays roles in CNS development in vertebrates. This article is part of a Special Issue entitled RNA-Binding Proteins. (c) 2012 Elsevier B.V. All rights reserved.
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