期刊
BRAIN RESEARCH
卷 1487, 期 -, 页码 69-77出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.brainres.2012.06.058
关键词
Electrical synapse; Synaptic plasticity; CaMKII
资金
- National Institutes of Health [NIH RO1 NS041282]
- German Research Council [DFG De 292/11]
The major neuronal gap junction protein connexin36 (Cx36) exhibits the remarkable property of run-up, in which junctional conductance typically increases by 10-fold or more within 5-10 min following cell break-in with patch pipettes. Such conductance run-up is a unique property of Cx36, as it has not been seen in cell pairs expressing other connexins. Because of the recent observation describing CaMKII binding and phosphorylation sites in Cx36 and evidence that calmodulin dependent protein kinase II (CaMKII) may potentiate electrical coupling in neurons of teleosts, we have explored whether CaMKII activates mammalian Cx36. Consistent with this hypothesis, certain Cx36 mutants lacking the CaMKII binding and phosphorylation sites or wild type Cx36 treated with certain cognate peptides corresponding to binding or phosphorylation sites blocked or strongly attenuated run-up of junctional conductance. Likewise, KN-93, an inhibitor of CaMKII, blocked run-up, as did a membrane permeable peptide corresponding to the CaMKII autoinhibitory domain. Furthermore, run-up was blocked by phosphatase delivered within the pipette and not affected by treatment with the phosphatase inhibitor okadaic acid. These results imply that phosphorylation by CaMKII strengthens junctional currents of Cx36 channels, thereby conferring functional plasticity on electrical synapses formed of this protein. (C) 2012 Elsevier B.V. All rights reserved.
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