期刊
BRAIN RESEARCH
卷 1370, 期 -, 页码 129-135出版社
ELSEVIER
DOI: 10.1016/j.brainres.2010.11.008
关键词
Central pain; Anxiety; Spinal cord injury; Place escape/avoidance paradigm (PEAP); Elevated plus maze; Animal behavior
资金
- Danish Agency for Science, Technology and Innovation
- Faculty of Health Sciences, Aarhus University
- Velux Fonden
- Ludvig and Sara Elsass Foundation
- Department of Clinical Medicine, Aarhus University
- Aarhus University Research Foundation
- Innovative Medicines Initiative Joint Undertaking (IMI JU) [115007]
Spinal cord injury (SCI) pain in humans is difficult to treat, and the lack of valid methods to measure behavior comparable to the complex human pain experience preclinically represents an important obstacle to finding better treatments for this type of central pain. The place escape/avoidance paradigm (PEAP) relies on the active choice of an animal between its natural preference for a dark environment or pain relief, and it has been suggested to measure the affective-motivational component of pain. We have modified the method to a T10 spinal cord contusion model (SCC) of at-level central neuropathic pain in Sprague-Dawley rats. In order to demonstrate sensitivity to change in escape/avoidance behavior and thus the applicability of the PEAP method to predict drug efficacy, we investigated the effect of pregabalin (30 mg/kg) treatment in a randomized design. SCC animals displayed increased escape/avoidance behavior postinjury, indicating at-level mechanical hypersensitivity. Second, we found no correlation between state anxiety levels in SCC animals (elevated plus maze) and PEAP behavior, suggesting that the PEAP measurement is not biased by differences in anxiety levels. Third, we demonstrated a decrease in escape/avoidance behavior in response to treatment with the analgesic drug pregabalin. Thus, the PEAP may be applicable as a surrogate correlate of human pain. In conclusion, the primary finding in this study was a sensitivity to change in escape/avoidance behavior induced by pharmacological modulation with analgesics, supporting the use of the PEAP as a central outcome measure in preclinical SCI pain research. (C) 2010 Elsevier B.V. All rights reserved.
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