期刊
BRAIN RESEARCH
卷 1343, 期 -, 页码 20-27出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.brainres.2010.04.043
关键词
Dopamine receptor; Glutamate release; Protein kinase C; SKF83959; Spontaneous excitatory; postsynaptic current; Transient receptor potential; vanilloid 1
资金
- Ministry of Science and Technology of China [2007AA02z163, 2009CB522001]
- Natural Science Foundation of China [20872153, 30825042, 30770662]
Central dopaminergic system exerts profound modulation on spontaneous glutamate release in various brain regions mainly through D-1 receptor/cAMP/PKA pathway. It remains unclear whether the phosphatidylinositol (PI)-linked D-1-like receptors are also involved in such modulatory actions. The identification of substituted phenylbenzazepine SKF83959 as the selective agonist for the atypical D-1-like receptors has given impetus to study their influence on the spontaneous glutamate release in the brain. In the present study the effects of SKF83959 on the spontaneous excitatory postsynaptic currents (sEPSCs) were investigated through whole-cell recording from layer V-VI pyramidal neurons in rat somatosensory cortical slices. Perfusion with SKF83959 (10-100 mu M) considerably increased the frequency of sEPSCs, while had no significant effect on the amplitude of sEPSCs. The increase of sEPSC frequency by SKF83959 was blocked by SCH23390, a D-1-like receptor antagonist, but not by the antagonists for D-2 receptor, alpha(1)-adrenoceptor and 5-HT2A/2C receptor. U-73122 (PLC beta inhibitor), 2-APB (IP3 receptor antagonist), chelerythrine chloride (PKC inhibitor) and capsazepine (TRPV1 antagonist) could block the effects of SKF83959, whereas H-89 (PKA inhibitor) and forskolin (adenylyl cyclase activator) had no effect. Taken together, sensitization of TRPV1 channels by PKC after activation of D-1 receptor/PLC beta signaling pathway mediated SKF83959-induced increase in the sEPSC frequency. To our knowledge, this is the first pharmacological evidence that PI-linked D-1-like dopamine receptors do exist in presynaptic terminals of cortical neurons and play an important role in controlling the spontaneous glutamate release. (C) 2010 Elsevier B.V. All rights reserved.
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