Article
Chemistry, Multidisciplinary
Cheng Ma, Zejing Xing, Xiaodan Gou, Li-Ping Jiang, Jun-Jie Zhu
Summary: This study develops a temperature-tuned ECL layer to image single cell topography at different heights by regulating the thickness of the ECL layer with electrode temperature. The ECL layer in two routes shows different regulation ranges with elevated temperature, providing a flexible approach to adjust the imaging scope within specific heights. Moreover, a heated electrode significantly improves the image quality of cell adhesion in heterogeneous electrochemical rate-determined situations. This work offers a new approach to regulate the ECL layer and has promising applications in monitoring transient heat generation from biological entities.
Review
Biochemistry & Molecular Biology
Erica Tagliatti, Katia Cortese
Summary: Endocytosis is a crucial process for cell growth and viability, involved in nutrient uptake, plasma membrane homeostasis, and defense against pathogens. Abnormal endocytosis has been associated with various human diseases. Advanced imaging techniques, such as fluorescence imaging and electron microscopy, have been developed to study endocytosis events in living cells and tissues. Understanding endocytosis and its imaging methodologies is important for unraveling cellular physiology and disease mechanisms.
Editorial Material
Biochemistry & Molecular Biology
Neda Bagheri, Anne E. Carpenter, Emma Lundberg, Anne L. Plant, Rick Horwitz
Summary: Quantitative optical microscopy has made significant advancements in single-cell biology, but its impact has been limited by challenges in data generation, management, and analysis. This article outlines these challenges and provides insights into the future of this field, highlighting the importance of data-driven microscopy and comparing it to the advances in genomics.
Article
Multidisciplinary Sciences
Alice Sandmeyer, Lili Wang, Wolfgang Huebner, Marcel Mueller, Benjamin K. Chen, Thomas Huser
Summary: Cost-effective, highly portable, and easy to use high-resolution live-cell imaging systems can revolutionize research in challenging environments, enabling real-time tracking of virus particles and studying infection mechanisms.
Article
Chemistry, Analytical
Zhaoyan Tian, Yafeng Wu, Fengying Shao, Dezhi Tang, Xiang Qin, Chenchen Wang, Songqin Liu
Summary: An integrated microfluidic system was designed for real-time monitoring of glycan expression on the surface of single tumor cells, achieving a level of 5.0 x 10^8 molecules per cell. This platform has the capability to distinguish single cancer cells from normal cells based on the expression level of glycans, providing a simple and accessible method for single-cell analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Cell Biology
Amy H. Ponsford, Thomas A. Ryan, Andrea Raimondi, Emanuele Cocucci, Susanne A. Wycislo, Florian Froehlich, Laura E. Swan, Massimiliano Stagi
Summary: Peripheral lysosomes are not less acidic compared to perinuclear lysosomes, and lysosomal pH has a certain impact on the transmission of lysosomal stress signals.
Article
Biotechnology & Applied Microbiology
Andreas Boden, Francesca Pennacchietti, Giovanna Coceano, Martina Damenti, Michael Ratz, Ilaria Testa
Summary: A new 3D pRESOLFT microscope method with sub-80-nm resolution has been developed to visualize the volumetric architecture of organelles and molecules inside whole living cells, allowing for targeted 3D confinement of fluorescence and observation of dynamic structural alterations in cells.
NATURE BIOTECHNOLOGY
(2021)
Article
Biophysics
Min Jeong Kim, Jinyong Park, Minjae Kang, Uidon Jeong, Dokyung Jeong, Nae-Gyu Kang, Seung Jin Hwang, Sung Hun Youn, Bo Kyoung Hwang, Yoonsuk Hyun, Doory Kim
Summary: The study developed novel approaches for the detection and identification of bacterial species in the skin microbiome using super-resolution microscopy and AI-based image analysis. These methods can differentiate between common bacterial members of the skin flora and different ribotypes of Cutibacterium acnes with single-cell sensitivity. The advantages of these methods, such as lack of time-consuming steps and high detection sensitivity, show promise for early diagnosis of bacterial infections and as a next-generation platform for microbiome detection as single-cell diagnostics.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Endocrinology & Metabolism
Zipeng A. A. Li, Jung Hoon Cho, Louis G. G. Woodhams, Jing W. W. Hughes
Summary: Primary cilia are slender cell-surface organelles that play important roles in regulating various cell responses in pancreatic beta cells, such as GPCR signaling, calcium influx, and insulin secretion. Visualization of primary cilia using fluorescence microscopy is crucial for studying their abundance, distribution, and morphology in pancreatic islets. Adopting fluorescence imaging methods, including live-cell imaging and quantitative analysis, can provide insights into the function of primary cilia in islet biology.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anayat Bhat, Shuang Li, Daniel Hammler, Martin J. Winterhalder, Andreas Marx, Andreas Zumbusch
Summary: The hydrolysis of nucleotides is important for cellular energy, and the transfer of phosphates to proteins is crucial for post-translational modification. A novel fluorogenic adenosine 5'-tetraphosphate (Ap4) analog has been developed for real-time monitoring of ATP hydrolysis within live cells, with potential applications in studying cellular processes involving Ap4 hydrolysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Yu-Jui Fan, Han-Yun Hsieh, Sheng-Fang Tsai, Cheng-Hsuan Wu, Chia-Ming Lee, Yen-Ting Liu, Chieh-Han Lu, Shu-Wei Chang, Bi-Chang Chen
Summary: The study developed a continuous cell-imaging system with subcellular resolution by integrating a microfluidic platform with lattice lightsheet microscopy (LLSM). By using a microfluidic channel sealed with a water refractive index-matched thin film, aberrations of the lightsheet entering the device were reduced, leading to clearer cell images.
Article
Chemistry, Multidisciplinary
Cheyenne J. Christopherson, Nathan R. Paisley, Zhujun Xiao, W. Russ Algar, Zachary M. Hudson
Summary: The newly developed orange/red-emitting polymer dots utilize thermally activated delayed fluorescence technology for time-gated imaging, inspired by the cell invasion mechanism of the HIV TAT protein. Pdots efficiently enter various cell types rapidly while maintaining low cellular toxicity, providing a new approach for live-cell fluorescence imaging.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Multidisciplinary Sciences
Chenfei Hu, Shenghua He, Young Jae Lee, Yuchen He, Edward M. Kong, Hua Li, Mark A. Anastasio, Gabriel Popescu
Summary: The study introduces a method for assessing cell viability without the need for labeling, utilizing deep learning techniques combined with quantitative phase imaging to accurately determine cell survival status without causing damage to the cells.
NATURE COMMUNICATIONS
(2022)
Article
Materials Science, Multidisciplinary
Franziska Weigl, Carina Blum, Ana Sancho, Juergen Groll
Summary: In recent decades, hybrid characterization systems have become important in cellular biomechanics research. This paper introduces a multifunctional device that combines Fluorescence Microscopy (FL) with FluidFM technology, allowing for real-time optical tracking of cell detachment processes while acquiring force-distance curves simultaneously.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Multidisciplinary Sciences
Rie Kamiyama, Kota Banzai, Peiwei Liu, Abhijit Marar, Ryo Tamura, Fangchao Jiang, Miyuki A. Fitch, Jin Xie, Daichi Kamiyama
Summary: The Drosophila experimental system significantly impacts modern biology studies, with the ability to label endogenously expressed proteins being crucial. Researchers have successfully developed a method using self-complementing split fluorescent proteins in Drosophila to label endogenous proteins in specific cell types and enhance fluorescence signals for subcellular live-cell imaging.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Neurosciences
Xiaomei Lin, Tianyuyi Feng, Erheng Cui, Yunfei Li, Zhang Qin, Xiaohu Zhao
Summary: This study successfully established a rat model based on the genetic-environmental interaction, which exhibited phenotype characteristics similar to human AD in terms of cognitive function, brain microstructure, and immunohistochemistry. The genetic factor (APP mutation) and the environmental factor (acrolein exposure) accounted for 39.74% and 33.3% of the AD-like phenotypes in the model, respectively.
Article
Neurosciences
Gustavo Guimara Guerrero, Giovanna Bignoto Minhoto, Camilla dos Santos Tiburcio-Machado, Itza Amarisis Ribeiro Pinto, Claudio Antonio Federico, Marcia Carneiro Valera
Summary: The present study evaluated the influence of head and neck radiotherapy on the behavior and body weight gain in Wistar rats. The results demonstrated that different doses of radiation induced depressive behavior in the animals, and that the weight gain tended to be lower in the irradiated groups.
Article
Neurosciences
Ziwei Gao, Chao Lu, Yaping Zhu, Yuxin Liu, Yuesong Lin, Wenming Gao, Liyuan Tian, Lei Wu
Summary: This study reveals the underlying mechanisms of the rapid antidepressant effects of merazin hydrate (MH), which activates CaMKII to promote neuronal activities and proliferation in the hippocampus.
Article
Neurosciences
Kathleen E. Murray, Whitney A. Ratliff, Vedad Delic, Bruce A. Citron
Summary: Gulf War Illness (GWI) is a chronic disorder that affects approximately 30% of Veterans deployed to the Persian Gulf. This study found that exposure to toxicants during the Gulf War resulted in long-term changes in the morphology of dentate granule cells and that treatment with Nrf2 activator could improve neuronal health in the hippocampus.
Article
Neurosciences
Jing Li, Yan Zou, Xiangchuang Kong, Yangming Leng, Fan Yang, Guofeng Zhou, Bo Liu, Wenliang Fan
Summary: This study examines the functional connectivity changes in individuals with sudden sensorineural hearing loss (SSNHL) at the integrity, network, and edge levels. The findings reveal reduced intranetwork connectivity strength and increased internetwork connectivity in SSNHL patients. These alterations are associated with the duration of SSNHL and Tinnitus Handicap Inventory scores. The study provides crucial insights into the neural mechanisms of SSNHL and the brain's network-level responses to sensory loss.
Review
Neurosciences
Didier Majou, Anne-Lise Dermenghem
Summary: In the early stages of SAD, memory impairment is strongly correlated with cortical levels of soluble amyloid-beta peptide oligomers. A beta disrupts glutamatergic synaptic function and leads to cognitive deficits. This article describes the pathogenic mechanisms underlying cerebral amyloidosis, involving amyloid precursor protein synthesis, A beta residue clearance processes, and the role of specific molecules.
Article
Neurosciences
Jing Li, Yi Shan, Xiaojing Zhao, Guixiang Shan, Peng-Hu Wei, Lin Liu, Changming Wang, Hang Wu, Weiqun Song, Yi Tang, Guo-Guang Zhao, Jie Lu
Summary: This study investigates changes in brain anatomical structures and functional network connectivity after chronic complete thoracic spinal cord injury (cctSCI) and their impact on clinical outcomes. The findings reveal alterations in gray matter volume and functional connectivity in specific brain regions, indicating potential therapeutic targets and methods for tracking treatment outcomes.
Article
Neurosciences
Anllely Fernandez, Katherine Corvalan, Octavia Santis, Maxs Mendez-Ruette, Ariel Caviedes, Matias Pizarro, Maria -Teresa Gomez, Luis Federico Batiz, Peter Landgraf, Thilo Kahne, Alejandro Rojas-Fernandez, Ursula Wyneken
Summary: This study reveals the importance of SUMOylation in modulating the protein cargo of astrocyte-derived small extracellular vesicles (sEVs) and its potential impact on neurons.
Article
Neurosciences
Anika Luettig, Stefanie Perl, Maria Zetsche, Franziska Richter, Denise Franz, Marco Heerdegen, Ruediger Koehling, Angelika Richter
Summary: This study found that changes in c-Fos activity during short-term stimulation of the entopeduncular nucleus (EPN) are associated with improvement in dystonia, and also discovered that the cerebellum may be involved in the antidystonic effects.
Article
Neurosciences
Yanlin Tao, Wei Shen, Houyuan Zhou, Zikang Li, Ting Pi, Hui Wu, Hailian Shi, Fei Huang, Xiaojun Wu
Summary: Depression has a higher incidence in women compared to men, and this study investigated the impact of sex on depressive behaviors and underlying mechanisms using a corticosterone-induced depression model in mice. The results showed sex-specific anxiety and depression behaviors in the model group, as well as differences in protein expression and neurotransmitter levels between male and female mice. These findings enhance our understanding of sex-specific differences in depression and support tailored interventions.
Review
Neurosciences
Dnyandev G. Gadhave, Vrashabh V. Sugandhi, Chandrakant R. Kokare
Summary: This article discusses the characteristics and importance of the tight junctions of endothelial cells in the CNS, which act as a biological barrier known as the blood-brain barrier (BBB). It focuses on overcoming the challenges of delivering therapeutic agents to the brain in neurodegenerative disorders, particularly multiple sclerosis, through the use of biomaterials. The article also highlights the current limitations of animal models for studying multiple sclerosis and suggests a potential future research direction.
Article
Neurosciences
Li-Min Mao, Khyathi Thallapureddy, John Q. Wang
Summary: Propofol can enhance synapsin phosphorylation and modulate synaptic transmission in the mouse brain. The study reveals the potential role of synapsin as a substrate of propofol and its effects on neurotransmitter release machinery.
Article
Neurosciences
Syed Maaz Ahmed Rizvi, Abdul Baseer Buriro, Irfan Ahmed, Abdul Aziz Memon
Summary: This study explores the effects of prolonged mask usage on the human brain by analyzing EEG and physiological parameters. The results show that the mean EEG spectral power in alpha, beta, and gamma sub-bands of individuals wearing masks is smaller than those without masks. The performances on cognitive tasks and oxygen saturation level differ between the two groups, while blood pressure, body temperature, and heart rate are similar. The analysis also reveals that the occipital and frontal lobes exhibit the greatest variability in channel measurements.
Article
Neurosciences
Rui-Fang Ma, Lu-Lu Xue, Jin-Xiang Liu, Li Chen, Liu-Lin Xiong, Ting-Hua Wang, Fei Liu
Summary: This study observed changes in brain infarction and blood vessels in rats during neonatal hypoxic ischemic encephalopathy (NHIE) modeling using Transcranial Doppler Ultrasonography (TCD). Longer duration of hypoxia was associated with more severe nerve damage. TCD can dynamically monitor cerebral infarction after NHIE modeling, which may serve as a useful auxiliary method for evaluating animal experimental models.
Article
Neurosciences
Yuxiang Dai, Chen Yu, Lu Zhou, Longyang Cheng, Hongbin Ni, Weibang Liang
Summary: Overexpression of CXCR4 in glioma is correlated with patient survival, and its inhibition can reduce invasion and migration of glioma cells. Inhibiting Nur77 also decreases cancer progression associated with CXCR4.