期刊
BRAIN PATHOLOGY
卷 20, 期 6, 页码 1055-1068出版社
WILEY
DOI: 10.1111/j.1750-3639.2010.00412.x
关键词
corpus callosum; membrane cytoskeleton; proteolysis; rat; traumatic axonal injury; traumatic brain injury
资金
- NIH [NS057758, NS056247]
- Virginia Commonwealth Neurotrauma Initiative [07-302F]
- NIH-NINDS [NS047463]
A high membrane-to-cytoplasm ratio makes axons particularly vulnerable to traumatic injury. Posttraumatic shifts in ionic homeostasis promote spectrin cleavage, disrupt ankyrin linkages and destabilize axolemmal proteins. This study contrasted ankyrin-G and alpha II-spectrin degradation in cortex and corpus callosum following diffuse axonal injury produced by fluid percussion insult. Ankyrin-G lysis occurred preferentially in white matter, with acute elevation of all fragments and long-term reduction of a low kD form. Calpain-generated alpha II-spectrin fragments increased in both regions. Caspase-3 lysis of alpha II-spectrin showed a small, acute rise in cortex but was absent in callosum. White matter displayed nodal damage, with horseradish peroxidase permeability into the submyelin space. Ankyrin-G-binding protein neurofascin and spectrin-binding protein ankyrin-B showed acute alterations in expression. These results support ankyrin-G vulnerability in white matter following trauma and suggest that ankyrin-G and alpha II-spectrin proteolysis disrupts Node of Ranvier integrity. The time course of such changes were comparable to previously observed functional deficits in callosal fibers.
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