Article
Immunology
Hong-Wei Sun, Wen-Chao Wu, Hai-Tian Chen, Yi-Tuo Xu, Yan-Yan Yang, Jing Chen, Xing-Juan Yu, Zilian Wang, Ze-Yu Shuang, Limin Zheng
Summary: The study demonstrates that glutamine deprivation can enhance the expression of G-CSF and GM-CSF in breast cancer cells, leading to generation of immunosuppressive myeloid-derived suppressor cells (MDSCs) due to impaired HPC-maintaining capacity in the bone marrow.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Jane Koo, Ashley Teusink-Cross, Stella M. Davies, Sonata Jodele, Christopher E. Dandoy
Summary: In pediatric solid tumor and lymphoma patients, those with heavily pretreated history may require more frequent dosing of G-CSF or combination with plerixafor for successful HSC mobilization. These methods can effectively improve HSC mobilization in the majority of cases.
PEDIATRIC BLOOD & CANCER
(2021)
Review
Hematology
Irene Garcia-Garcia, Joan Cid, Gloria Carbasse, Javier Lopez-Jimenez, Gemma Moreno, Miquel Lozano
Summary: A retrospective multicenter analysis of HPCs mobilization procedures was conducted to investigate the potential benefits of high dose G-CSF. The results showed that compared to the standard dose, high dose G-CSF did not significantly improve the mobilization of CD34+ cells in healthy donors or patients, and did not reduce the incidence of poor mobilization.
TRANSFUSION MEDICINE REVIEWS
(2022)
Article
Hematology
Abdulrahman Theyab, Mohammad Algahtani, Khalaf F. Alsharif, Yousef M. Hawsawi, Abdulaziz Alghamdi, Adel Alghamdi, Jude Akinwale
Summary: G-CSF has drawn researchers' attention as a therapeutic agent for neutropenia patients, but its daily injections pose inconvenience. Understanding its structure, expression, and mechanism is crucial for more effective treatment.
Article
Biochemistry & Molecular Biology
Flavy Roseren, Martine Pithioux, Stephane Robert, Laure Balasse, Benjamin Guillet, Edouard Lamy, Sandrine Roffino
Summary: The study showed that G-CSF can accelerate bone regeneration and modulate the mobilization of progenitor cells during the process of distraction osteogenesis, promoting bone consolidation. In the early phase of consolidation, the G-CSF group exhibited lower osteoclast activity and a trend of increased osteoblast activity, while significantly lower neovascularization was observed in the late phase of consolidation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Rong Wang, Man Chen, Minjing Fu, Wei Zhao, Jing Zhou, Meiwei Gong, Qingqing Wu, Hui Wang
Summary: This study discusses the effects of mobilizing healthy donors with G-CSF on the absolute values and functions of MDSCs and their subpopulations in peripheral blood, as well as the impacts of transferring MDSCs from the graft to patients on their prognosis and immune reconstitution. The results show that G-CSF significantly increases the levels of MDSCs in the peripheral blood of donors, and the level of transferred P-MDSCs has a significant impact on the prognosis of patients. Maintaining a balanced state of MDSCs is crucial for effective immunotherapy.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biophysics
Kristina Hoelig, Helmuth Schmidt, Gero Huetter, Michael Kramer, Raphael Teipel, Katharina Heidrich, Kristin Zimmer, Falk Heidenreich, Matthias Blechschmidt, Tigran Torosian, Rainer Ordemann, Frank Kroschinsky, Elke Ruecker-Braun, Laszlo Gopsca, Eva Maria Wagner-Drouet, Uta Oelschlaegel, Alexander H. Schmidt, Martin Bornhaeuser, Gerhard Ehninger, Johannes Schetelig
Summary: In allogeneic PBSC donors with poor mobilization response to G-CSF, the study found that the use of plerixafor (P) can effectively increase the yield of CD34+ cells, helping to achieve the target cell count without severe adverse events.
BONE MARROW TRANSPLANTATION
(2021)
Article
Cell Biology
Kohei Kemuriyama, Jianbo An, Satoru Motoyama, Yushi Nagaki, Tomokazu Yamaguchi, Yusuke Sato, Akiyuki Wakita, Yoshihiro Minamiya, Keiji Kuba
Summary: In this study, the researchers found that tumor-derived G-CSF promotes disease progression in various types of cancers. High expression of G-CSF in esophageal SCC (ESCC) tumor tissues is associated with poor prognosis. Deletion of G-CSF in tumor cells mitigated tumor growth and metastasis in mice. Mechanistically, G-CSF enhances cell proliferation in vitro and its accumulation in mice leads to an expansion of neutrophils and a decrease in CD8(+) T cells. Antibody depletion of neutrophils partially suppresses tumor growth but has no effect on distant metastasis. The findings suggest that G-CSF produced by tumor cells facilitates tumor progression through promoting neutrophil recruitment and tumor cell proliferation.
Article
Hematology
Joan Cid, Silvia Monsalvo, Carlos Castillo, Cristina Pascual, Gemma Moreno-Jim, Miriam Lopez-Parra, Concepcion Andon, Luisa Guerra, Albert Esquirol, Isabel Sanchez-Ortega, Sandra Ortega, Saioa Zalba, Carmen Martinez, Montserrat Rovira, Pedro Marin, Miquel Lozano
Summary: Addition of plerixafor after G-CSF mobilization failure in HRDs allowed collecting higher number of CD34+ cells compared to steady-state mobilization.
TRANSFUSION AND APHERESIS SCIENCE
(2021)
Letter
Cardiac & Cardiovascular Systems
Wenjuan Pu, Mingjun Zhang, Xiuxiu Liu, Lingjuan He, Jie Li, Ximeng Han, Kathy O. Lui, Ben He, Bin Zhou
Review
Cell Biology
Wendong Weng, Xiuxiu Liu, Kathy O. Lui, Bin Zhou
Summary: Accurate deciphering of cellular plasticity in vivo is crucial for understanding biological processes. Site-specific recombinases are genetic tools used for in vivo lineage tracing and gene manipulation. Different recombinase systems can be combined to increase precision, allowing for lineage tracing, studying cellular heterogeneity, recording cellular activities, and even genome editing.
TRENDS IN CELL BIOLOGY
(2022)
Review
Cardiac & Cardiovascular Systems
Maoying Han, Bin Zhou
Summary: This review focuses on the important role of cardiac fibroblasts in cardiac injury and repair, including their origin, dynamic cellular states after injury, and heterogeneity. Recent findings suggest that manipulating specific populations of fibroblasts could mitigate cardiac fibrosis and promote cardiac repair.
CURRENT CARDIOLOGY REPORTS
(2022)
Letter
Cell Biology
Yi Li, Huan Zhu, Qianyu Zhang, Ximeng Han, Zhenqian Zhang, Linghong Shen, Lixin Wang, Kathy O. Lui, Ben He, Bin Zhou
Letter
Cardiac & Cardiovascular Systems
Maoying Han, Zixin Liu, Lingjuan He, Xufeng Li, Lei Liu, Xiuzhen Huang, Mingjun Zhang, Yan Yan, Kathy O. Lui, Bin Zhou
Article
Cardiac & Cardiovascular Systems
Hengwei Jin, Kuo Liu, Xiuzhen Huang, Huanhuan Huo, Jialing Mou, Zengyong Qiao, Ben He, Bin Zhou
Summary: This study provides evidence that GPCMs minimally invade the injured heart after myocardial infarction and do not prevent cardiac fibrosis or exhibit reparative function.
CIRCULATION RESEARCH
(2022)
Article
Multidisciplinary Sciences
Yang Liu, Qi Chen, Hyun-Woo Jeong, Bong Ihn Koh, Emma C. Watson, Cong Xu, Martin Stehling, Bin Zhou, Ralf H. Adams
Summary: The colonization of bone marrow by hematopoietic stem and progenitor cells is critical for blood cell formation throughout life. This study reveals distinct features of fetal bone marrow and identifies artery-derived signals, such as Wnt2, that promote hematopoietic colonization. The findings suggest a fundamental switch in the cellular organization and molecular regulation of bone marrow niches between embryonic and adult organisms, and provide insights into improving transplantation outcomes.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Zhenqian Zhang, Bin Zhou
Summary: It has been reported recently that there are two distinct subpopulations of capillary endothelial cells in the mammalian lungs: gCap (general capillary) and aCap (aerocyte). They are identified by two unique markers, respectively: plasmalemmal vesicle-associated protein (PLVAP) and carbonic anhydrase IV (CAR4). Novel knock-in mouse lines Plvap-CreER and Car4-CreER are reported here, which genetically target gCap and aCap, respectively. These mouse lines mediate specific and efficient Cre-loxP recombinations in PLVAP thorn gCap and CAR4 thorn aCap, respectively, in the lungs, and can be used as useful genetic tools to investigate cell fates and functions of PLVAP thorn and CAR4 thorn cells in lung homeostasis, injury and repair.
JOURNAL OF GENETICS AND GENOMICS
(2022)
Article
Multidisciplinary Sciences
Shaohua Zhang, Qianyu Zhang, Zixin Liu, Kuo Liu, Lingjuan He, Kathy O. Lui, Lixin Wang, Bin Zhou
Summary: Understanding cell-cell interactions is crucial for understanding biological processes. In this study, we developed a labeling strategy called Cre-induced intercellular labeling protein (CILP) to label neighboring cells in mammals. By using CILP, we successfully labeled endothelial cells in adult mice and revealed their gene signatures.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Shaohua Zhang, Huan Zhao, Zixin Liu, Kuo Liu, Huan Zhu, Wenjuan Pu, Lingjuan He, Rong A. Wang, Bin Zhou
Summary: Monitoring cell-cell communication is crucial for understanding biological processes. Researchers have developed a genetic technology that can track and label cell-cell contacts, allowing for the study of dynamic cell-cell interactions in vivo.
Letter
Cell Biology
Mingjun Zhang, Wenjuan Pu, Jie Li, Maoying Han, Ximeng Han, Zhenqian Zhang, Zan Lv, Nicola Smart, Lixin Wang, Bin Zhou
Article
Cell Biology
Kuo Liu, Hengwei Jin, Shaohua Zhang, Muxue Tang, Xinfeng Meng, Yan Li, Wenjuan Pu, Kathy O. Lui, Bin Zhou
Summary: Cardiac resident macrophages are important for heart development, homeostasis, repair, and regeneration. Recent studies have suggested the hematopoietic potential of cardiac endothelium, but previous genetic tracing studies did not strongly support this due to limitations in tracing tools. In this study, a new intercellular genetic labeling system was developed to trace heart-specific endothelial cells, and the results show that cardiac endothelial cells do not have hemogenic potential and do not contribute to cardiac macrophages or circulating blood cells.
DEVELOPMENTAL CELL
(2023)
Review
Biochemical Research Methods
Xiuxiu Liu, Wendong Weng, Lingjuan He, Bin Zhou
Summary: The experimental measurement of cell proliferation is essential for understanding the origins of cells that drive the development of organs, tissue regeneration, and repair. We have recently developed a genetic approach called ProTracer that allows lifelong, noninvasive monitoring of cell proliferation in specific cell lineages in live animals. This system provides a detailed protocol for studying cell proliferation and can be easily implemented by researchers skilled in mouse-related experiments.
Correction
Cell Biology
Mingjun Zhang, Wenjuan Pu, Jie Li, Maoying Han, Ximeng Han, Zhenqian Zhang, Zan Lv, Nicola Smart, Lixin Wang, Bin Zhou
Letter
Cardiac & Cardiovascular Systems
Jingdong Wu, Hong Zhao, Yanmeng Tao, Chunyan Yang, Yang Yang, Bin Zhou, Yang Zhao