Article
Biotechnology & Applied Microbiology
Koh Meng Aw Yong, Eric Horst, Dylan Neale, Sonya Royzenblat, Joerg Lahann, Colin Greineder, Megan Weivoda, Geeta Mehta, Evan T. Keller
Summary: The bone is a common metastatic site for prostate cancer, but the mechanism of interaction between the tumor and the bone microenvironment is not fully understood. In this study, a tunable bioreactor was developed to culture osteocyte cells and study their response to pressure. The results demonstrate that the bioreactor can mimic the mechanotransduction response of osteocytes, providing a useful tool for studying the mechanisms of osteocytes in bone health and disease.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Materials Science, Ceramics
L. Stipniece, S. Wilson, J. M. Curran, R. Chen, K. Salma-Ancane, P. K. Sharma, B. J. Meenan, A. R. Boyd
Summary: This study synthesized hydroxyapatite and Sr-substituted hydroxyapatite powders with varying levels of Sr inclusion. It was found that higher Sr content in SrHAp directly promoted the maturation of osteoblasts into osteocytes compared to hydroxyapatite.
CERAMICS INTERNATIONAL
(2021)
Article
Cell Biology
Katharina Wirsig, David Kilian, Max von Witzleben, Michael Gelinsky, Anne Bernhardt
Summary: This study investigates the effects of Sr2+ and hypoxic cultivation on bone cells using an in vitro bone triple culture model. The results show that Sr2+ stimulates ALP activity in osteoblasts, but reduces gene marker expression in osteoblasts and osteocytes, while enhancing activity of osteoclast-specific enzymes. Hypoxic cultivation leads to decreased DNA content in all bone cells, as well as downregulated gene marker expression in osteoblasts and osteocytes.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Article
Engineering, Biomedical
Tadaaki Matsuzaka, Aira Matsugaki, Takayoshi Nakano
Summary: The study elucidated the relationship between mechanical stimuli and the formation of anisotropic collagen/apatite microstructure, as well as the role of prostaglandin E2 in oriented collagen/apatite organization in bone tissue.
Article
Biochemistry & Molecular Biology
Anne Bernhardt, Jasmin Skottke, Max von Witzleben, Michael Gelinsky
Summary: In this study, a 3D co-culture model was established with primary human osteoblasts, osteoclasts, and osteocytes, providing preliminary information on the performance of bone graft materials by analyzing the interactions of different cell types. The experiment showed typical morphology and gene expression of various markers in the three cell species, as well as enzyme activities relevant to osteoblasts and osteoclasts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Multidisciplinary
Tzu-Cheng Sung, Chao-Wen Heish, Henry Hsin-Chung Lee, Jhe-Yu Hsu, Chun-Ko Wang, Jia-Hua Wang, Yu-Ru Zhu, Shih-Hsi Jen, Shih-Tien Hsu, Abdurahman H. Hirad, Abdullah A. Alarfaj, Akon Higuchi
Summary: This study shows that the pluripotency and differentiation ability of human adipose-derived stem cells (hASCs) can be reversibly changed during sequential cultivation with 2D and 3D culturing processes, with at least a 2-fold enhancement in pluripotency observed in hASCs cultivated with a 3D culturing process after 2D culture. The environment of stem cell culture (2D or 3D cultivation) can greatly impact stem cell fate in terms of pluripotency and differentiation reversibility.
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
(2021)
Review
Endocrinology & Metabolism
Jialiang S. Wang, Courtney M. Mazur, Marc N. Wein
Summary: The skeleton serves as an endocrine organ controlling mineral metabolism and energy homeostasis. Different bone cell types secrete factors that affect multiple target organs, with osteocalcin and sclerostin playing key roles.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Meng Chen, Wenyu Fu, Huiyun Xu, Chuan-ju Liu
Summary: Glucocorticoid (GC) is commonly used to treat inflammatory and autoimmune diseases, but its high doses and long-term usage can lead to adverse effects, especially glucocorticoid-induced osteoporosis (GIO). Excessive GCs have harmful effects on bone cells, inhibiting osteoblast proliferation and differentiation, enhancing osteoblast and osteocyte apoptosis, and promoting osteoclastogenesis and bone resorption. GCs also disrupt the secretion of bone cells, affecting the process of osteoblastogenesis and osteoclastogenesis.
CYTOKINE & GROWTH FACTOR REVIEWS
(2023)
Review
Medicine, General & Internal
Kok-Yong Chin, Ben Nett Ng, Muhd Khairik Imran Rostam, Nur Farah Dhaniyah Muhammad Fadzil, Vaishnavi Raman, Farzana Mohamed Yunus, Syed Alhafiz Syed Hashim, Sophia Ogechi Ekeuku
Summary: This review provides an overview of osteoporosis, including its biology, epidemiology, and clinical aspects, serving as an updated reference for readers to understand the basics of the disease and take preventive and management actions.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Endocrinology & Metabolism
Narelle E. McGregor, Emma C. Walker, Audrey S. M. Chan, Ingrid J. Poulton, Ellie H-J Cho, Sara H. Windahl, Natalie A. Sims
Summary: Mice with targeted SOCS3 deletion exhibit an exaggerated response to mechanical loads, both physiological and experimental, highlighting the importance of osteocytic response in cortical bone maturation and the potential for enhanced bone formation by increasing STAT3 signaling within osteocytes.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Jun Yang, Wuju Zhang, Eryong Lai, Wen Liu, Pinglin Lai, Zhipeng Zou, Weidong Wang, Xiaochun Bai
Summary: Rheb1 plays an important role in bone metabolism, with Rheb1 KO mice showing impaired bone microarchitecture, inhibited bone formation, and increased bone resorption.
DNA AND CELL BIOLOGY
(2022)
Article
Environmental Sciences
Yonggang Ma, Di Ran, Xueni Shi, Hongyan Zhao, Zongping Liu
Summary: Cadmium is a widespread environmental contaminant that causes severe bone metabolism disease and dental issues. It affects bone cell and teeth development, leading to oxidative stress and DNA damage, ultimately resulting in cell apoptosis.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Engineering, Biomedical
Jianhua Zhang, Julia Griesbach, Marsel Ganeyev, Anna-Katharina Zehnder, Peng Zeng, Gian Nutal Schaedli, Anke de Leeuw, Yuxiao Lai, Marina Rubert, Ralph Mueller
Summary: The study reveals that mechanical stimulation can promote the formation of 3D bioprinted bone organoids, and long-term mechanical loading is necessary for increasing the density and stiffness of the organoids, as well as promoting osteoblast differentiation.
Article
Biochemistry & Molecular Biology
Makoto Izumiya, Miyu Haniu, Katsuya Ueda, Haruka Ishida, Chuang Ma, Hirokazu Ideta, Atsushi Sobajima, Koki Ueshiba, Takeshi Uemura, Naoto Saito, Hisao Haniu
Summary: The study evaluated the osteogenesis of MC3T3-E1 cells under different culture media conditions, measuring cell proliferation, alkaline phosphatase activity, expression of osteoblast markers, and calcification. The research found that the type of base medium, the presence or absence of vitamin C, and the freshness of the medium may impact biomaterial regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Endocrinology & Metabolism
Lilian I. Plotkin, Natasha Sanz, Lucas R. Brun
Summary: Bone is an important dynamic tissue that influences bone mass and skeletal complications through the activity of bone-forming and bone-resorbing cells. The interaction among bone cells occurs through direct contact and secreted molecules, which can also have effects in other tissues and organs. Bone cell products have been associated with the establishment and progression of diseases, including cancer and muscle weakness.
CALCIFIED TISSUE INTERNATIONAL
(2023)
Letter
Hematology
Keisuke Kidoguchi, Masako Yokoo, Akihisa Umino, Shigehisa Aoki, Shinya Kimura
ANNALS OF HEMATOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Yusuke Yakushiji, Kazuhiro Kawamoto, Kazuyoshi Uchihashi, Masafumi Ihara, Shigehisa Aoki, Yukiko Nagaishi, Kohei Suzuyama, Yumiko Tsugitomi, Hideo Hara
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Multidisciplinary Sciences
Fumiya Tao, Kanae Sayo, Kazuyuki Sugimoto, Shigehisa Aoki, Nobuhiko Kojima
SCIENTIFIC REPORTS
(2020)
Article
Oncology
Kei Nagase, Takashi Akutagawa, Mihoko Rikitake-Yamamoto, Sayuri Morito, Maki Futamata, Shohei Tobu, Mitsuru Noguchi, Shuji Toda, Shigehisa Aoki
Summary: This study investigated the cellular and physical microenvironments of RCC, exploring their regulatory effects on RCC behavior. The results showed that adipose tissue, macrophages, and fluid flow influence the proliferative and invasive capacity of RCC cells, and these effects may be mediated through the extracellular signal-regulated kinase and p38 signaling pathways.
JOURNAL OF PATHOLOGY
(2021)
Review
Oncology
Junzo Yamaguchi, Hiroki Moriuchi, Takashi Ueda, Yujo Kawashita, Takanori Hazeyama, Masaki Tateishi, Shigehisa Aoki, Kazuyoshi Uchihashi, Mikio Nakamura
Summary: The study found that adipose tissue invasion was associated with poor survival in triple-negative breast cancer (TNBC), suggesting that cancer cell invasion into local fat may play a crucial role in TNBC progression and systemic disease development.
Article
Cell & Tissue Engineering
Mariko Hashiguchi, Toshiaki Takezawa, Kei Nagase, Mizuki Tayama-Abe, Fumikazu Matsuhisa, Shuji Kitajima, Sayuri Morito, Kotaro Yamaji, Maki Futamata, Yasuhisa Sakata, Takashi Akutagawa, Masatoshi Yokoyama, Shuji Toda, Shigehisa Aoki
Summary: This study evaluates the use of an indwelling collagen vitrigel membrane-coated nylon line to prevent cervical stenosis after conization. The results show that this device effectively prevents cervical canal stenosis and cystic changes by inhibiting fibrosis, myofibroblast emergence, connective tissue growth factor expression, and collagen type III deposition in the regenerating cervix. The findings suggest that this device has the potential to be a new method for preventing cervical canal fibrosis and stenosis after conization for cervical cancer.
TISSUE ENGINEERING PART A
(2021)
Article
Cell Biology
Katsuyuki Hanashima, Takashi Akutagawa, Mihoko Yamamoto-Rikitake, Takehisa Sakumoto, Maki Futamata, Yoshifumi Nakao, Masatoshi Yokoyama, Shuji Toda, Shigehisa Aoki
Summary: The study investigates the regulatory effects of the physical and biological microenvironments on the aggressiveness and survival of cervical SCC cells. Results show that dynamic flow, air exposure, and stromal fibroblasts influence the growth, apoptosis, and invasiveness of cervical cancer cells, and modulate the extracellular signal-regulated kinase and p38 signaling pathways in a cell-type dependent manner.
ACTA HISTOCHEMICA ET CYTOCHEMICA
(2021)
Article
Cell Biology
Maki Kawasaki, Kei Nagase, Shigehisa Aoki, Kazuma Udo, Shohei Tobu, Mihoko Rikitake-Yamamoto, Masaya Kubota, Takayuki Narita, Mitsuru Noguchi
Summary: This study investigated the bystander effect of irradiated adipose tissue-derived stromal cells (ATSCs) on urothelial carcinoma (UC) cells. The results showed that irradiated ATSCs inhibited the growth and promoted the apoptosis of UC cells, while increasing their invasiveness. Furthermore, irradiated ATSCs regulated the expression of various proteins in UC. The findings suggest that the bystander effect of irradiated ATSCs is a critical regulator of UC and its actions differ depending on the type of mesenchymal cell involved.
Article
Dentistry, Oral Surgery & Medicine
Atsushi Danjo, Reona Aijima, Reiko U. Yoshimoto, Shin-Ichi Tanaka, Shuhei Iwamoto, Takeshi Katsuki, Shigehisa Aoki, Mizuho A. Kido, Yoshio Yamashita
Summary: This study compared bone healing after osteotomy with ultrasonic osteotomy devices (UODs) and rotary osteotomy devices (RODs). The results showed that UODs had smoother cuts, faster bone healing, and greater quantity of newly formed bone compared to RODs. Osteoblast-like cells harvested from UOD-cut bone chips had higher proliferative activity. It is presumed that UODs stimulate the differentiation of mesenchymal cells into osteoblasts and cause less damage to the bone, thereby assisting bone regeneration.
JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY MEDICINE AND PATHOLOGY
(2022)
Article
Cell Biology
Shuhei Iwamoto, Megumi Nishiyama, Maki Kawasaki, Sayuri Morito, Takehisa Sakumoto, Shuji Toda, Yoshio Yamashita, Shigehisa Aoki
Summary: Squamous cell carcinoma (SCC) is the most common malignant tumor of the tongue, and understanding the cellular and physical microenvironments of tongue SCC is important. In this study, a collagen cell disc was developed to simulate cancer-stroma interactions and fluid flow stimulation in vitro. The behavior of tongue SCC was found to be influenced by cell-cell interactions and fluid flow stimulation, with the latter enhancing the anti-proliferative effect of cisplatin on SCC cells. The newly established model could be a promising tool for studying tongue SCC and developing treatment strategies for oral cancer.
Article
Cell Biology
Sayuri Morito, Maki Kawasaki, Megumi Nishiyama, Takehisa Sakumoto, Mariko Hashiguchi, Takayuki Narita, Atsushi Kawaguchi, Shuji Toda, Shigehisa Aoki
Summary: The importance of the microenvironment in regulating cancer cell behavior and drug sensitivity is widely recognized. In this study, the specificities of endometrioid carcinoma, including obesity (biological), fluid flow (physical), and anticancer agents (chemical), were investigated to target the microenvironmental elements of the carcinoma. A tissue culture model was developed to demonstrate the individual and synergistic effects of these elements on endometrioid carcinoma. The model revealed that adipose tissue induced chemoresistance, but fluid flow abolished this effect, indicating the potential of this model for understanding the mechanisms and developing treatment strategies for endometrioid carcinoma.
Article
Polymer Science
Yu Ishibashi, Ryota Haraguchi, Shigehisa Aoki, Yushi Oishi, Takayuki Narita
Summary: Hollow collagen gels treated with UV have the potential to function as carriers for drug/cell delivery systems. The UV treatment thickens the pre-gel solutions and prevents over-swelling of the hollow collagen rods. The prepared collagen hollow fiber rods have a large lumen space and limited swelling ratio for separate cell culture.
Article
Pathology
Keita Kai, Mariko Hashiguchi, Makiko Kurihara, Yuya Tanaka, Sho Kitamura, Mitsuo Nakamura, Chika Shichijo, Mihoko Yamamoto-Rikitake, Ryoichi Okuma, Takako Hikari, Emi Okuma, Michiko Uchiyama, Shigehisa Aoki, Yoshifumi Nakao, Masatoshi Yokoyama, Shinichi Aishima
Summary: SMBT is a rare neoplasm with cytological characteristics that can be diagnosed through cytological examination. Cytological examination of ascites and cell block examination play a key role in diagnosing SMBT.
Article
Medicine, General & Internal
Keisuke Kidoguchi, Hiroo Katsuya, Hiroshi Ureshino, Haruna Kizuka-Sano, Kyosuke Yamaguchi, Ayako Nagata, Shuichi Rikitake, Kanako Aikawa, Shinji Naito, Shigehisa Aoki, Yasushi Kubota, Toshihiko Ando, Shinya Kimura
TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE
(2020)
Article
Urology & Nephrology
Tohru Mizumasa, Kazuho Honda, Shigehisa Aoki, Chieko Hamada, Masanobu Miyazaki, Yasuhiko Ito, Yudo Tanno, Toshiaki Nakano, Masaaki Nakayama
RENAL REPLACEMENT THERAPY
(2020)
Review
Endocrinology & Metabolism
Jemima E. Schadow, David Maxey, Toby O. Smith, Mikko A. J. Finnila, Sarah L. Manske, Neil A. Segal, Andy Kin On Wong, Rachel A. Davey, Tom Turmezei, Kathryn S. Stok
Summary: This study systematically reviewed the published parameters for assessing subchondral bone in human osteoarthritis using computed tomography. The study identified clinically meaningful parameter categories and emphasized the importance of quantification and standardized measurement methods for improving the evaluation of disease progression.
Article
Endocrinology & Metabolism
Lindsay L. Loundagin, Kim D. Harrison, Xuan Wei, David M. L. Cooper
Summary: This study developed new techniques to define zones of BMU activity based on the 3D morphology of remodeling spaces in rabbit cortical bone and integrated morphological data with the BMU longitudinal erosion rate (LER) to elucidate the spatial-temporal coordination of BMUs and estimate mineral apposition rate (MAR). The results showed that the manual and semi-automated methods accurately defined the zones of remodeling spaces, and these techniques have the potential to assess dynamic parameters of bone resorption and formation.
Article
Endocrinology & Metabolism
Soroush Masrouri, Farzad Esmaeili, Maryam Tohidi, Fereidoun Azizi, Farzad Hadaegh
Summary: This study examined the association between estimated glomerular filtration rate (eGFR) decline and fracture incidence. The results showed that rapid kidney function decline (RKFD) can increase the incidence of fractures among the general population.
Article
Endocrinology & Metabolism
Steven J. Meas, Gabriella M. Daire, Michael A. Friedman, Rachel Denapoli, Preetam Ghosh, Joshua N. Farr, Henry J. Donahue
Summary: Age- and disuse-related bone loss both lead to decreases in bone mineral density, cortical thickness, and trabecular thickness and connectivity. It is important to experimentally compare these two mechanisms at a structural and transcriptomic level to better understand their similarities and differences. This study compares the effects of hindlimb unloading and aging on bone microarchitecture and gene expression in mice, finding that while both induce similar changes, aging has a greater impact on the transcriptome and tissue level.
Correction
Endocrinology & Metabolism
Masaru Matsuoka, Sho Tsukamoto, Yuta Orihara, Rieko Kawamura, Mai Kuratani, Nobuhiko Haga, Kenji Ikebuchi, Takenobu Katagiri
Article
Endocrinology & Metabolism
Rachel Kohler, Amy Creecy, David R. Williams, Matthew R. Allen, Joseph M. Wallace
Summary: Osteogenesis imperfecta is a hereditary bone disease that weakens bones and increase fracture risk. Current interventions mainly focus on increasing bone mass, but the compromised tissue-level material properties are not addressed. A study found that a RAL analog could reduce fracture risk, but further development is needed for optimal results in patients with osteogenesis imperfecta.
Article
Endocrinology & Metabolism
So Jeong Park, Eunhye Ji, Hyun Ju Yoo, Kyunggon Kim, Sunghwan Ji, Ji Yeon Baek, Jin Young Lee, Hee-Won Jung, Il-Young Jang, Eunju Lee, Namki Hong, Beom-Jun Kim
Summary: The study analyzed the relationship between serum lumican levels and osteosarcopenia in older adults, showing that older adults with osteosarcopenia had lower serum lumican levels. Lower serum lumican levels were associated with reduced bone mass and grip strength, indicating that lumican levels could be used as a biomarker for assessing the risk of osteosarcopenia, osteoporosis, or sarcopenia in older adults.
Article
Endocrinology & Metabolism
Michael B. Chavez, Michelle H. Tan, Tamara N. Kolli, Natalie L. Andras, Brian L. Foster
Summary: This study revealed the complex mechanisms by which disabling BSP functional domains led to profound and distinct changes in cementoblast cell functions, including dysregulated gene expression and reduced mineralization.
Article
Endocrinology & Metabolism
Julien Seiller, Blandine Merle, Romain Fort, Emilie Virot, Solene Poutrel, Giovanna Cannas, Arnaud Hot, Roland Chapurlat
Summary: The purpose of this study was to assess the prevalence of bone fragility in sickle cell patients and to evaluate the potential risk factors and associated complications.
Article
Endocrinology & Metabolism
Chirantap Oza, Anuradha Khadilkar, Pranay Goel, Madhura Karguppikar, Nikhil Shah, Nikhil Lohiya, Shruti Mondkar, Prashant Patil, Hemchand Prasad, Ankita Maheshwari, Dipali Ladkat, Neha Kajale, Chidvilas More, Devarati Khurjekar, Vaman Khadilkar
Summary: This study revealed that BoneXpert (BX) can be used for accurate assessment of bone age and screening of bone health in Indian children and youth with type-1 diabetes (T1D). 51.5% of T1D subjects showed significantly decreased metacarpal index (MCI). Height, Tanner stage, and vitamin D concentrations were positively correlated with MCI, while HbA1c and disease duration were negatively correlated with MCI.
Article
Endocrinology & Metabolism
Mariam R. Farman, Catherine Rehder, Theodora Malli, Cheryl Rockman-Greenberg, Kathryn Dahir, Gabriel Angel Martos-Moreno, Agnes Linglart, Keiichi Ozono, Lothar Seefried, Guillermo del Angel, Gerald Webersinke, Francesca Barbazza, Lisa K. John, Sewmi M. A. Delana Mudiyanselage, Florian Hoegler, Erica Burner Nading, Erin Huggins, Eric T. Rush, Ahmed El-Gazzar, Priya S. Kishnani, Wolfgang Hoegler
Summary: The ALPL gene variant database serves as an archive for interpreting the clinical significance of ALPL gene variants, facilitating the reclassification of VUS and continuous updates. The project establishes an international expert consortium, providing a multidisciplinary collaboration framework to improve genetic counseling and medical decision-making for HPP patients.
Article
Endocrinology & Metabolism
Giovanni Adami, Davide Gatti, Maurizio Rossini, Alessandro Giollo, Matteo Gatti, Francesco Bertoldo, Eugenia Bertoldo, Amy S. Mudano, Kenneth G. Saag, Ombretta Viapiana, Angelo Fassio
Summary: Certain diseases requiring glucocorticoids are independently associated with an increased risk of fractures. Chronic obstructive pulmonary disease (COPD) and neurological diseases are associated with both vertebral and non-vertebral fracture risk, while rheumatoid arthritis (RA) and inflammatory bowel disease (IBD) are only associated with non-vertebral fractures.
Article
Endocrinology & Metabolism
Frank C. Ko, Rong Xie, Brandon Willis, Zoe G. Herdman, Bryan A. Dulion, Hoomin Lee, Chun-do Oh, Di Chen, D. Rick Sumner
Summary: Intramembranous bone regeneration is important in joint and tooth replacement, but its underlying mechanisms are not well understood. This study found that increased periostin gene expression preceded increases in osteogenic genes during bone regeneration. Using a genetic mouse model, the researchers discovered that cells transiently expressing periostin played a critical role in intramedullary intramembranous bone regeneration.
Article
Endocrinology & Metabolism
T. Savikangas, T. H. Suominen, M. Alen, T. Rantalainen, S. Sipila
Summary: Regular exercise, especially high-intensity physical activity, can help slow down age-related bone loss and prevent a decline in femoral neck bone mineral density.
Article
Endocrinology & Metabolism
Mishaela R. Rubin, Ruban Dhaliwal
Summary: The increased risk of fractures observed in adults with type 1 diabetes (T1D) cannot be solely explained by modest decreases in areal bone mineral density (BMD). Accumulation of advanced glycation endproducts (AGEs) in bone has been suggested as a possible cause for the increased bone fragility in diabetes. Although the evidence linking AGEs and fractures in individuals with T1D is limited, recent data show that AGEs, as measured by skin intrinsic fluorescence, are a risk factor for lower BMD in T1D. Further research is needed to determine if there is a causal relationship between fractures and AGEs in T1D. If confirmed, this could lead to interventions that can reduce AGE accumulation and ultimately reduce fractures in T1D patients.