期刊
BONE
卷 44, 期 2, 页码 306-315出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.bone.2008.09.005
关键词
Growth plate; Matrix proteins; Static loading; Rodent animal model; In vivo
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Canada Research Chair in Mechanobiology of the Pediatric Musculoskeletal System
- Yves Cotrel Foundation
Mechanical loads are essential to normal bone growth, but excessive loads can lead to progressive deformities. In addition, growth plate extracellular matrix remodelling is essential to regulate the normal longitudinal bone growth process and to ensure physiological bone mineralization. In order to investigate the effects of static compression on growth plate extracellular matrix using an in vivo animal model, a loading device was used to precisely apply a compressive stress of 0.2 MPa for two weeks on the seventh caudal vertebra (Cd7) of rats during the pubertal growth spurt. Control, sham and loaded groups were studied. Growth modulation was quantified based on calcein labelling, and three matrix components (type II and X collagens, and aggrecan) were assessed using immunohistochemistry/safranin-O staining. As well, extracellular matrix components and enzymes (MMP-3 and -13, ADAMTS-4 and -5) were studied by qRT-PCR. Loading reduced Cd7 growth by 29% (p < 0.05) and 15% (p = 0.07) when compared to controls and shams respectively. No significant change could be observed in the mRNA expression of collagens and the proteolytic enzyme MMP-13. However, MMP-3 was significantly increased in the loaded group as compared to the control group (p < 0.05). No change was observed in aggrecan and ADAMTS-4 and -5 expression. Low immunostaining for type II and X collagens was observed in 83% of the loaded rats as compared to the control rats. This in vivo study shows that, during pubertal growth spurt, two-week static compression reduced caudal vertebrae growth rates; this mechanical growth modulation occurred with decreased type 11 and X collagen proteins in the growth plate. (C) 2008 Elsevier Inc. All rights reserved.
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