Article
Multidisciplinary Sciences
Iva Cvitas, Simone Oberhaensli, Tosso Leeb, Eliane Marti
Summary: Equine insect bite hypersensitivity (IBH) is a common skin disease in horses caused by an IgE-mediated hypersensitivity reaction to salivary gland proteins of Culicoides insects. Keratinocytes play an important role in the development of IBH, contributing to the innate immune response and responding to different stimuli.
Review
Immunology
Melanie Humeau, Katia Boniface, Charles Bodet
Summary: Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by dysregulated immune response and increased colonization of Staphylococcus aureus. The interaction between T cells and keratinocytes through cytokine release plays a key role in AD pathogenesis, with potential impact from virulence factors produced by Staphylococcus aureus.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
R. J. Thibodeaux, J. A. Brady, C. C. K. Maryak, S. L. Swiger, B. W. Jones
Summary: Digital dermatitis is a bacterial infection affecting cattle hooves, with Treponema species suspected as causative agents. This research aimed to investigate if flies captured from a dairy farm with digital dermatitis were contaminated with Treponema bacteria, but DNA-based assays did not detect any contamination. Other potential means of bacterial transmission were also discussed.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Ingrid H. Holmoy, Lina Ahlen, Jenny Frossling, Liv Solverod, Menno Holzhauer, Ane Nodtvedt, Terje Fjeldaas
Summary: The study evaluated the accuracy of two ELISA tests for detecting Treponema antibodies associated with digital dermatitis in Norwegian dairy cattle herds. Both tests were able to detect the antibodies, but did not achieve satisfactory sensitivity without compromising specificity. Inspection at claw trimming in a chute remains necessary for surveillance and control of digital dermatitis at the herd level in Norway.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Cell Biology
Matthew G. Brewer, Stephanie R. Monticelli, Mary C. Moran, Benjamin L. Miller, Lisa A. Beck, Brian M. Ward
Summary: Individuals with atopic dermatitis (AD) are more susceptible to viral infections, but little is known about how the inflammatory environment affects the susceptibility of skin cells to viruses. This study found that under conditions simulating AD, keratinocytes become more susceptible to vaccinia virus (the causative agent of eczema vaccinatum), and this susceptibility can be reduced by inhibiting JAK.
Article
Multidisciplinary Sciences
A. S. Vermeersch, M. Ali, Y. Gansemans, F. Van Nieuwerburgh, P. Geldhof, R. Ducatelle, D. Deforce, J. Callens, G. Opsomer
Summary: This study is the first to explore the changes in gene expression in severe udder cleft dermatitis lesions (UCD) in Holstein-Friesian cows. RNA Seq-Technology was used to compare gene expression levels in UCD lesions and healthy udder skin, revealing significant differences in inflammatory pathways. Upregulated genes included chemokines CXCL2, CXCL5, CXCL8, and CCL11, as well as multifunctional molecules ADAM12 and SLPI, while downregulated genes were associated with keratins. Pathway analysis showed significant upregulation of inflammatory pathways such as chemokine signaling, cytokine receptor interaction, and IL-17 signaling. These findings suggest a dysregulated inflammatory response, disrupted skin barrier integrity, and impaired wound repair mechanism in severe UCD lesions.
Article
Microbiology
Nivia Canales, Hedie Bustamante, Jennifer Wilson-Welder, Cristian Thomas, Emilio Ramirez, Miguel Salgado
Summary: Digital dermatitis (DD) is a highly contagious and infectious disease in cattle that has negative economic impact and affects animal welfare globally. This study confirms the presence of Treponema spp. DNA in Chilean dairy cattle with DD, providing valuable information for future herd control plans.
Article
Biochemistry & Molecular Biology
Saeko Nakajima, Duerna Tie, Takashi Nomura, Kenji Kabashima
Summary: Type 2 immunity and inflammation play key roles in allergic skin disorders like atopic dermatitis, with cytokines such as IL-4 and IL-13 being essential in type 2 inflammation. Biologics targeting these cytokines have shown significant efficacy in treating AD, based on both basic research in mice and clinical trials in humans.
Article
Agriculture, Dairy & Animal Science
A. Anagnostopoulos, M. Barden, J. Tulloch, K. Williams, B. Griffiths, C. Bedford, M. Rudd, A. Psifidi, G. Banos, G. Oikonomou
Summary: Using infrared thermography, this study aimed to investigate the relationship between interdigital skin temperature (IST) and different stages of digital dermatitis (DD) lesions in cows, as well as develop models for identifying cows with active DD lesions. The results showed that IST was associated with DD lesions, with the highest temperatures observed in feet with M2 lesions. Logistic regression models using IST measurements demonstrated potential for detecting the presence of active DD lesions, with an average area under the curve of 0.80 and varying sensitivity and specificity levels across different time points.
JOURNAL OF DAIRY SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Raffaele Brancaccio, Giuseppe Murdaca, Rossella Casella, Teresa Loverre, Laura Bonzano, Eustachio Nettis, Sebastiano Gangemi
Summary: Skin inflammation is a common feature of atopic dermatitis, allergic contact dermatitis and chronic spontaneous urticaria, and the pathogenetic mechanisms are not fully understood. This study reviews the role of miRNA in regulating inflammatory mechanisms and innate and adaptive immune responses in these skin conditions. miRNAs are found to be involved in the pathogenesis and regulation of atopic dermatitis, chronic spontaneous urticaria, and allergic contact dermatitis, and they could serve as biomarkers and potential therapeutic targets for these chronic skin conditions.
Article
Agriculture, Dairy & Animal Science
R. C. Joubert, H. F. Strydom, E. van Marle-Koster
Summary: Claw lesions in dairy cows play a significant role in causing lameness and welfare concerns. This study analyzed claw-trimming data from dairy farms in South Africa to examine the occurrence and recording of claw lesions in Holstein cattle. The results highlight the complexity of lesion data and suggest the need to simplify the recording and categorization of claw lesions to aid in the prevention and treatment of lameness.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2023)
Article
Agriculture, Dairy & Animal Science
A. Vanhoudt, J. Hesseling, M. Nielen, J. Wilmink, R. Jorritsma, T. van Werven
Summary: This clinical trial compared the efficacy of an enzyme alginogel and a coppergel in treating active digital dermatitis lesions on dairy cattle hind feet. The results showed that lesions treated with the alginogel had a lower improvement rate in M-score, while most lesions treated with coppergel transitioned to chronic lesions. Additionally, a high percentage of lesions treated with coppergel had unscorable wound healing progress. Future research should focus on successful wound management of digital dermatitis lesions to alleviate pain and reduce disease transmission and prevalence within herds.
JOURNAL OF DAIRY SCIENCE
(2022)
Review
Immunology
Shin Morizane, Tomoyuki Mukai, Ko Sunagawa, Kota Tachibana, Yoshio Kawakami, Mamoru Ouchida
Summary: This review aims to provide a foundation of knowledge on the cytokines recognized or produced by epidermal keratinocytes, deepening our understanding of the role of cytokines in inflammatory skin diseases.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Dermatology
Taras Lyubchenko, Hannah K. Collins, Kathryn A. Vang, Donald Y. M. Leung, Elena Goleva
Summary: IL-4 and IL-13 have been shown to modulate keratinocyte sensitivity to changes in extracellular calcium levels, inhibiting differentiation signals by suppressing the expression of the Ca2+-binding protein SMOC1. These findings suggest a new target for the treatment of atopic dermatitis.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Lai-San Wong, Yu-Ta Yen, Chih-Hung Lee
Summary: Atopic dermatitis is a prototypic inflammatory disease characterized by intense itching, with multifactorial pathophysiology involving environmental factors, genetic susceptibility, skin barrier function, and immune responses. Recent research has shown that pruritogens not only trigger itch but also interact with immune cells and act as inflammatory mediators, worsening the severity of atopic dermatitis. Targeted therapies for atopic dermatitis focus on the interaction between pruritogens and inflammatory molecules.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Gastroenterology & Hepatology
Rita Matos, Hugo Santos Sousa, Jorge Nogueiro, Ana Magalhaes, Celso A. Reis, Fatima Carneiro, Irina Amorim, Freddy Haesebrouck, Fatima Gartner
Summary: The study shows that gastric non-Helicobacter pylori Helicobacter can bind to the human gastric mucosa, with a prevalence of chronic gastritis and chronic active gastritis among patients. Interestingly, these bacteria were found to bind to the gastric mucosa of individuals with severe chronic inflammation and intestinal metaplasia.
Article
Microbiology
Bruno Toledo-Silva, Lisa Beuckelaere, Anneleen De Visscher, Chloe Geeroms, Evelyne Meyer, Sofie Piepers, Damien Thiry, Freddy Haesebrouck, Sarne De Vliegher
Summary: This paper presents a new quantitative method for evaluating and quantifying the in vitro growth inhibition of mastitis-related bacteria. The method can accurately quantify the differences in growth inhibition among different NAS isolates.
Article
Health Care Sciences & Services
Rita Matos, Emily Taillieu, Sofie De Bruyckere, Chloe De Witte, Alexandra Rema, Hugo Santos-Sousa, Jorge Nogueiro, Celso A. Reis, Fatima Carneiro, Freddy Haesebrouck, Irina Amorim, Fatima Gaertner
Summary: This study reveals the presence of Helicobacter spp. in 46.3% of gastric cancer and obese patients, with 43.8% having single H. pylori infection and 2.5% having mixed infections with H. pylori and H. felis associated with dogs or cats. The effectiveness of current Helicobacter eradication treatments needs further improvement.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Review
Biotechnology & Applied Microbiology
Junhua Xie, Qiqiong Li, Freddy Haesebrouck, Lien Van Hoecke, Roosmarijn E. Vandenbroucke
Summary: This article reviews the recent advances and obstacles in the use of bacterial extracellular vesicles (bEVs) in biomedical applications, and discusses the paths towards clinical translation.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Microbiology
Robin Temmerman, Mahdi Ghanbari, Gunther Antonissen, Gerd Schatzmayr, Luc Duchateau, Freddy Haesebrouck, An Garmyn, Mathias Devreese
Summary: Fluoroquinolone agents are critical for human medicine, but their use in poultry production may contribute to the spread of antibiotic resistance genes. This study used metagenomics to investigate the effects of fluoroquinolone application in the gut microbiome of broiler chickens. The results showed that low-dose fluoroquinolone had the most significant impact on the chicken gut microbiota and resistome, while synbiotic supplementation alleviated the burden of antibiotic resistance genes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Masahiko Nakamura, Futa Murasato, Anders Overby, Yosuke Kodama, Hirofumi Michimae, Kazuki Sasaki, Bram Flahou, Freddy Haesebrouck, Somay Y. Murayama, Shinichi Takahashi, Masayuki Uchida, Hidekazu Suzuki, Hidenori Matsui
Summary: The increased pH induced by acid suppressants affects the viability of non-Helicobacter pylori helicobacters within the cells and lumina of the stomach. Acid suppressants, such as vonoprazan, cause damage and apoptosis of the helicobacter cells by altering the pH.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Veterinary Sciences
Evelien Biebaut, Ilias Chantziaras, Filip Boyen, Bert Devriendt, Freddy Haesebrouck, Charles-Oliver Gomez-Duran, Dominiek Maes
Summary: This study investigated the presence of Mycoplasma hyopneumoniae in the breeding population of ten Belgian farrow-to-finish farms. The results showed that the prevalence of M. hyopneumoniae PCR varied greatly between farms, with gilts being more likely to be infected than sows. Positive animals were mostly found at 30-40 days of gestation. This highlights the importance of a sufficiently long quarantine period and proper gilt acclimation practices before introducing gilts to the sow herd.
PORCINE HEALTH MANAGEMENT
(2022)
Article
Physiology
Annatachja De Grande, Richard Ducatelle, Saskia Leleu, Christof Rapp, Cibele Torres, Massimiliano Petracci, Stefaan De Smet, Joris Michiels, Freddy Haesebrouck, Filip Van Immerseel, Evelyne Delezie
Summary: This study evaluated the effect of different zinc sources and vitamin E levels on meat yield and quality in broilers subjected to chronic cyclic heat stress. The results showed that replacing ZnSO4 with ZnAA increased breast meat weight and yield, as well as improved the water-holding capacity of the meat. The study also found that the beneficial effects of ZnAA were independent of the vitamin E level.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Junhua Xie, Freddy Haesebrouck, Lien Van Hoecke, Roosmarijn E. Vandenbroucke
Summary: Bacterial extracellular vesicles (bEVs) have been shown to play a crucial role in the pathogenesis of various diseases. This article thoroughly discusses the contribution of bEVs in disease pathogenesis, their potential as diagnostic biomarkers, and their application as therapeutic targets.
TRENDS IN MICROBIOLOGY
(2023)
Article
Infectious Diseases
Francisco Cortez Nunes, Emily Taillieu, Teresa Letra Mateus, Silvia Teixeira, Freddy Haesebrouck, Irina Amorim
Summary: This study aimed to assess the presence of antibiotic resistance genes previously reported in Helicobacter pylori in gastric samples from 36 pigs where DNA of H. pylori-like organisms had been detected. Based on PCR and sequencing analysis, two samples were positive for the 16S rRNA mutation gene conferring tetracycline resistance, and one sample was positive for the frxA gene conferring metronidazole resistance. These findings indicate the potential occurrence of acquired antimicrobial resistance in H. pylori-like organisms associated with pigs.
Meeting Abstract
Gastroenterology & Hepatology
Emily Taillieu, Sophie Rutten, Stijn Michiels, Yuna Arnst, Sofie De Bruyckere, Koen Chiers, Frauke van Aert, Heiko De Schepper, Emma Callewaert, Christophe George, Wouter Van Moerkercke, Glenn Vanneste, Nina Van Heddegem, Erik Vanderstraeten, Freddy Haesebrouck, Christophe Van Steenkiste
Review
Veterinary Sciences
Emily Taillieu, Koen Chiers, Irina Amorim, Fatima Gaertner, Dominiek Maes, Christophe Van Steenkiste, Freddy Haesebrouck
Summary: This article discusses the importance of Helicobacter species in dogs, cats, and pigs and their potential to infect humans. The article highlights the association of Helicobacter suis with gastritis and decreased weight gain in pigs, as well as its potential to cause disease in humans. It also mentions that multiple Helicobacter species can colonize the stomachs of dogs and cats, but their pathogenic significance is likely low. The article concludes that besides H. pylori, gastric Helicobacter species associated with these animals are also clinically relevant in humans.
VETERINARY RESEARCH
(2022)
Review
Gastroenterology & Hepatology
L. Capirchio, T-D. Huang, C. De Witte, F. Haesebrouck, C. Fervaille, C. Gillain, J-F. Rahier, T. De Ronde
Summary: This article reports the first case of elevated CA 19-9 related to H. suis gastritis, which normalized after eradication. In addition to H. pylori, infection with NHPH species can also cause elevated CA 19-9.
ACTA GASTRO-ENTEROLOGICA BELGICA
(2022)
Article
Veterinary Sciences
Silvia Teixeira, Dulce Filipe, Manuela Cerqueira, Patricia Barradas, Francisco Cortez Nunes, Fatima Faria, Freddy Haesebrouck, Joao R. Mesquita, Fatima Gartner, Irina Amorim
Summary: Cats serve as a natural reservoir for many non-Helicobacter pylori Helicobacters, and close contact with animals has been identified as a risk factor for gastric diseases caused by these bacteria. The prevalence of Helicobacter species in cats was determined, with Helicobacter heilmannii and Helicobacter salomonis being the most commonly detected species. Significant histopathological alterations associated with the presence of Helicobacter spp. were not observed in most cases, suggesting potential adaptation of these bacteria to the feline gastric microenvironment.
VETERINARY SCIENCES
(2022)
Article
Veterinary Sciences
Karina Sonalio, Henrique M. S. Almeida, Marina L. Mechler-Dreibi, Gabriel Y. Storino, Freddy Haesebrouck, Dominiek Maes, Luis Guilherme de Oliveira
Summary: The study found that Mycoplasma hyopneumoniae infection affects the diversity of respiratory microbiota and facilitates colonization of other pathogens. Differences in pulmonary and nasal microbiota were observed in pigs with different infection statuses.
VETERINARY RESEARCH
(2022)