Article
Plant Sciences
Claudia Gutierrez-Garcia, Shiek S. S. J. Ahmed, Sathishkumar Ramalingam, Dhivya Selvaraj, Aashish Srivastava, Sujay Paul, Ashutosh Sharma
Summary: This study utilized high-throughput sequencing technology to analyze the miRNA profile of Murraya koenigii and identified miRNAs associated with secondary metabolite biosynthesis. The results showed that these miRNAs targeted key enzymes in the terpenoid backbone and flavonoid biosynthesis pathways. The sequencing results were validated by qPCR. This is the first report of the miRNA profile of Murraya koenigii, providing valuable information for studying the involvement of miRNAs in secondary metabolism.
Article
Microbiology
Puspa M. Sharma, Nirmal K. Thapa, Pema Tshomo, Tshewang Dema, Cristian A. Alvarez Rojas, Tenzin Tenzin, Ratna B. Gurung, Tshering Norbu, Lhatru Lhatru, Phurpa Namgyel, Chimi Jamtsho, Kinzang Dukpa, Yoenten Phuentshok, Krishna P. Sharma, Sonam Pelden, Peter Deplazes
Summary: This study in Bhutan documented the occurrence and genetic diversity of Echinococcus and Taenia species, showing contamination of urban areas and pastures with Echinococcus eggs. Different Echinococcus haplotypes were identified, highlighting the need for a national control program due to the zoonotic feature and economic impact of these parasites in Bhutan.
Review
Parasitology
Antonio Varcasia, Claudia Tamponi, Fahad Ahmed, Maria Grazia Cappai, Francesca Porcu, Naunain Mehmood, Giorgia Dessi, Antonio Scala
Summary: Taenia multiceps is a taeniid cestode that causes Coenurosis, a worldwide distributed disease that results in substantial economic losses in livestock farming. The disease mainly affects young sheep and is characterized by either acute or chronic manifestations. The best diagnostic method involves clinical signs interpretation and diagnostic imaging techniques. A vaccine based on oncosphere antigens and use of anthelmintics during parasite migration stages have shown efficacy against T. multiceps infection in sheep. Surgery is the most effective treatment, but its usage is limited due to financial constraints. Preventative measures such as anthelmintic treatment of dogs and proper disposal of intermediate host carcasses can effectively control the disease. Coenurosis is also zoonotic and has been reported in humans with Coenuri located in the brain, spinal cord, and eyes.
PARASITES & VECTORS
(2022)
Article
Biochemistry & Molecular Biology
Michele Salemi, Giovanna Marchese, Giuseppe Lanza, Filomena I. I. Cosentino, Maria Grazia Salluzzo, Francesca A. Schillaci, Giovanna Maria Ventola, Angela Cordella, Maria Ravo, Raffaele Ferri
Summary: Parkinson's disease (PD) is a neurodegenerative synucleinopathy with an unclear molecular pathomechanism. This study analyzed miRNAs on peripheral blood mononuclear cells from 30 participants to identify differentially expressed miRNAs in PD compared to healthy controls. The study also explored the pathways influenced by these miRNAs and their potential association with PD susceptibility. Several dysregulated miRNAs and their putative targets were identified, suggesting their potential as diagnostic markers and outcome measures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Parasitology
Grazia Biosa, Piero Bonelli, Salvatore Pisanu, Stefania Ghisaura, Cinzia Santucciu, Angela Peruzzu, Giovanni Garippa, Sergio Uzzau, Giovanna Masala, Daniela Pagnozzi
Summary: The proteome of hydatid cyst fluids of Echinococcus granulosus and other ovine parasites were analyzed, and potential diagnostic biomarkers for cystic echinococcosis (CE) were identified, which can predict CE in sheep and differentiate between different types of parasites.
Article
Agriculture, Dairy & Animal Science
Xiaozhan Lin, Changxu Tian, Yang Huang, Hongjuan Shi, Guangli Li
Summary: The study identified differentially expressed genes (DEGs) related to pattern formation in spotted scat, revealing key genes and pathways associated with pigment synthesis and pigmentation. Understanding the molecular mechanisms of skin pigmentation differentiation in spotted scat will provide valuable information for skin coloration in other marine fish species.
Article
Biochemistry & Molecular Biology
Eman K. Aldous, Salman M. Toor, Aijaz Parray, Yasser Al-Sarraj, Ilhame Diboun, Essam M. Abdelalim, Abdelilah Arredouani, Omar El-Agnaf, Paul J. Thornalley, Naveed Akhtar, Sajitha Pananchikkal, Ashfaq Shuaib, Nehad M. Alajez, Omar M. E. Albagha
Summary: This study conducted comprehensive circulating miRNA profiling in patients with ischemic stroke and identified a panel of miRNAs with potential diagnostic and prognostic significance. A multivariate classifier based on this miRNA panel showed high sensitivity and specificity in differentiating between stroke patients and healthy controls, and it outperformed other available biomarkers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Jacek Kabzinski, Monika Maczynska, Ireneusz Majsterek
Summary: This article discusses the potential use of microRNAs in the diagnosis of head and neck squamous cell carcinoma, highlighting their application in radiotherapy and immunotherapy, as well as addressing the challenges and future research directions in developing this promising technology.
Article
Biotechnology & Applied Microbiology
Long-Fei Jin, Rajesh Yarra, Xin-Xing Yin, Yong-Zhong Liu, Hong-Xing Cao
Summary: Iron is a critical micronutrient for plant growth and development, and its deficiency can limit crop productivity. This study identified Fe-deficiency-responsive miRNAs in citrus, which may contribute to Fe-deficiency tolerance in citrus plants through regulation of multiple target genes.
Article
Veterinary Sciences
Yeshi Wangdi, Kesang Wangchuk
Summary: The research investigated yak herdsmen's practices to manage Coenurosis in the Laya administrative block of Bhutan, revealing the disease as a major cause of yak mortality. While most herders lost one to three yaks annually, the majority had not attended any awareness programs on the disease.
VETERINARY MEDICINE AND SCIENCE
(2021)
Article
Veterinary Sciences
Wen-Hui Li, Yang Yang, Nian-Zhang Zhang, Jian-Kui Wang, Yin-Ju Liu, Li Li, Hong-Bin Yan, Wan-Zhong Jia, Baoquan Fu
Summary: This study utilized RNA sequencing to investigate the transcriptome profiles of different stages of the life cycle of Taenia multiceps, providing insights into the mechanisms of development and parasitic life of the parasite. The results revealed a large number of differentially expressed genes involved in cellular and metabolic processes, binding and catalytic activity, genetic information processing, and environmental information processing, as well as genes related to development and parasite-host interaction.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Minh Ngoc Le, Cong Truc Le, Tuan Anh Nguyen
Summary: This article introduces a new method, intramolecular ligation method (iLIME), for quantifying and sequencing hairpin-containing pre-miRNAs. The method converts pre-miRNAs into circularized RNAs using T4 RNA ligase 1, allowing for PCR primer design for quantification and next-generation sequencing for end sequencing. This method offers a simple and effective solution for studying pre-miRNAs.
Article
Biochemistry & Molecular Biology
Maia Segura-Wang, Bertrand Grenier, Suzana Ilic, Ursula Ruczizka, Maximiliane Dippel, Moritz Buenger, Matthias Hackl, Veronika Nagl
Summary: This study investigated the changes in miRNA expression induced by DON in porcine liver, jejunum, and serum. The serum miRNA response to DON increased over time and declined after removal of contaminated diets. Receiver operating curve analyses for individual miRNAs were significant, and a combination of four miRNAs increased the predictive capacity for DON exposure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Don M. Matshazi, Cecil J. Weale, Rajiv T. Erasmus, Andre P. Kengne, Saarah F. G. Davids, Shanel Raghubeer, Glenda M. Davison, Tandi E. Matsha
Summary: The study on novel microRNAs in an African population revealed that some microRNAs are dysregulated in high blood pressure status, suggesting their potential as biomarker targets for diagnosis or treatment of hypertension.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Genetics & Heredity
Mengfeng Liu, Xiran Yu, Jianlong Bu, Qifan Xiao, Sitong Ma, Naozhong Chen, Changfa Qu
Summary: This study found that miRNAs in salivary exosomes may serve as biomarkers for the prediction and diagnosis of lung cancer. By miRNA sequencing and qPCR validation, differentially expressed miRNAs between lung cancer and non-lung cancer groups were identified, which were associated with pathways related to cancer.
FRONTIERS IN GENETICS
(2023)