Article
Chemistry, Multidisciplinary
Elena Porumb-Andrese, Ramona Gabriela Ursu, Iuliu Ivanov, Irina-Draga Caruntu, Vlad Porumb, Dan Ferariu, Costin Damian, Delia Ciobanu, Cristina Terinte, Luminita Smaranda Iancu
Summary: The study identified a high prevalence (60%) of BRAF V600E mutation in Romanian patients with primary cutaneous melanoma, with the mutation being associated with age, type of melanoma, and location on the body.
APPLIED SCIENCES-BASEL
(2021)
Article
Medicine, Research & Experimental
Clemens Cammann, Jonas Kulla, Christian Walz, Fang Zhao, Theresa Lowinus, Eylin Topfstedt, Neha Mishra, Petra Henklein, Ursula Bommhardt, Lukas Bossaller, Christian Hagemeier, Dirk Schadendorf, Boris Schmidt, Annette Paschen, Ulrike Seifert
Summary: The combination of proteasome and Kv1.3 potassium channel inhibitors can inhibit the growth of BRAF-mutant melanoma cells and induce apoptosis. This combination therapy may serve as an alternative approach to treat other tumors expressing mutated BRAF.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Oncology
Walid Shalata, Rachel Steckbeck, Ilya Polishchuk, Ahron Yehonatan Cohen, Keren Rouvinov, Margarita Tokar, Ashraf Abu Jama, Omar Abu Saleh, Kim Sheva, Alexander Yakobson
Summary: This case report presents a patient with metastatic melanoma complicated by hyperbilirubinemia. The patient had BRAF V600E-mutated melanoma with metastases in various organs. Due to a lack of guidelines for treating melanoma patients with hyperbilirubinemia, a panel of specialists discussed between treatment initiation and supportive care. The patient eventually received combination therapy with dabrafenib and trametinib, resulting in a significant therapeutic and radiological response.
FRONTIERS IN ONCOLOGY
(2023)
Review
Oncology
Hiroyuki Takeda, Yu Sunakawa
Summary: BRAF mutations are an important poor prognostic factor in mCRC, but greater understanding of patient characteristics through genomic classification allows for more ideal treatment strategies.
FRONTIERS IN ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Mingguo Xie, Xiongfei Wang, Jiao Qiao, Jian Zhou, Yuguang Guan, Tianfu Li, Xueling Qi, Guoming Luan
Summary: The study evaluated the clinicopathological features and surgical prognosis of epilepsy-associated glioneuronal tumors (GNT) with CD34 expression and BRAF mutation. The results showed that CD34 expression or BRAF mutation in GNT was not associated with surgical outcomes of seizure control and tumor progression-free survival.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Filipa Alves da Costa, Adriana Ramos, Catarina Bernardo, Fabio Cardoso Borges, Ana Costa Miranda
Summary: This is a population-based cohort study conducted in the South Region of Portugal to estimate the incidence of cutaneous malignant melanoma, characterize diagnosed individuals, describe treatment, and estimate survival outcomes. The study found a melanoma incidence rate of 13.36/100,000 inhabitants and a 3-year overall survival rate of 80.54%. The adjusted hazard ratio showed a two-fold increased risk of recurrence/death in the presence of ulcerated tumors.
SCIENTIFIC REPORTS
(2023)
Article
Medicine, General & Internal
Yun-Suk Choi, Seong-Woon Choi, Jin-Wook Yi
Summary: This study identified TERT promoter mutations in PTC and found that they are associated with more aggressive behavior. Patients with TERT promoter mutations were older, had larger tumors, and exhibited other adverse clinical features, resulting in a poorer prognosis compared to those with wild-type TERT gene status.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Medicine, General & Internal
Guodong Fu, Ronald S. Chazen, Christina MacMillan, Ian J. Witterick
Summary: This diagnostic study developed a sensitive molecular assay for detection and quantification of BRAF V600E variation in residual tissue from thyroid FNA biopsies to identify patients with cancer harboring BRAF V600E in a cost-effective manner, highlighting the clinical value of molecular assay of the remaining FNA tissue in the management of thyroid nodules.
Article
Chemistry, Analytical
Zhaocheng Liu, Rui Zhang, Xixi Jiang, Li Ji, Ping Sun, Yong Ji, Yu Zhang, Yan Ding, Koukou Li, Zhening Pu, Fengsheng Zhou, Jian Zou
Summary: Gene mutations are constantly accumulating in human cells. Detecting these mutations early on is crucial in physiological and pathological processes. A novel strategy called the hairpin competition amplification (HCA) system, using quantitative polymerase chain reaction (qPCR) and Sanger sequencing, has been developed to enrich rare mutations. HCA qPCR and Sanger sequencing demonstrated high sensitivity and temperature robustness, making it an effective tool for precision diagnosis and treatment of rare variant diseases.
ANALYTICAL CHEMISTRY
(2023)
Article
Oncology
Youjia Tian, Jiang Zhou, Xinxin Chai, Zejun Ping, Yurong Zhao, Xin Xu, Chi Luo, Jinghao Sheng
Summary: In this study, the researchers found that TCF12 plays an oncogenic role in melanoma progression. They observed that TCF12 expression increases as melanoma progresses, and high expression is associated with poor survival outcomes in melanoma patients. Functionally, TCF12 enhances melanoma proliferation and metastasis, as well as sensitivity to BRAF(V600E)-targeted therapy. The researchers also identified TGFB2 as a direct downstream target of TCF12, mediating its pro-tumorigenic effects.
Article
Oncology
Emilie Hafliger, Alessandra Boccaccino, Alexandra Lapeyre-Prost, Audrey Perret, Claire Gallois, Maria Antista, Lorenzo Pilla, Thierry Lecomte, Mario Scartozzi, Emilie Soularue, Lisa Salvatore, Vincent Bourgeois, Massimiliano Salati, David Tougeron, Ludovic Evesque, Jean-Nicolas Vaillant, Reem El-Khoury, Sara Lonardi, Chiara Cremolini, Julien Taieb
Summary: The combination of anti-BRAF and anti-EGFRs shows efficacy in patients with BRAFm mCRC previously treated with an anti-EGFR agent. The results of this retrospective study suggest that this combination could be a valuable treatment option for patients with limited therapeutic alternatives.
EUROPEAN JOURNAL OF CANCER
(2022)
Article
Oncology
Jianing Jiang, Jinqi Gao, Gang Wang, Jinyan Lv, Wenting Chen, Jing Ben, Ruoyu Wang
Summary: This study reported the case of a NSCLC patient with lung papillary carcinoma BM harboring a BRAF(V600E) mutation, benefiting from dabrafenib combined with trametinib and remaining effective with vemurafenib following the development of the BRAF(S365L) mutation. Additionally, the study found that NGS of CSF ctDNA may provide more accurate information about intracranial lesions compared to ctDNA in blood serum, indicating a potentially better detection method.
FRONTIERS IN ONCOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Abdullah O. Ba-Salem, Jean Duhamel
Summary: Py-3-12 is a cationic gemini surfactant with a low critical micellar concentration (CMC) and exhibits different fluorescence properties in aqueous solution.
Article
Dermatology
Li Ni, Pin Li, Mingming Li, Shuhong Huang, Ningning Dang
Summary: This study characterized the function and interaction of ITGAX, SERPINB8, and furin in BRAF V600E mutant melanoma. It was found that SERPINB8 and furin regulate the expression of ITGAX, which significantly promotes the proliferation and invasion of melanoma cells.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Oncology
Emerik Osterlund, Ari Ristimaki, Markus J. Makinen, Soili Kytola, Juha Kononen, Per Pfeiffer, Leena-Maija Soveri, Mauri Keinanen, Halfdan Sorbye, Luis Nunes, Tapio Salminen, Lasse Nieminen, Aki Uutela, Paivi Halonen, Annika Algars, Jari Sundstrom, Raija Kallio, Raija Ristamaki, Annamarja Lamminmaki, Hanna Stedt, Eetu Heerva, Teijo Kuopio, Tobias Sjoblom, Helena Isoniemi, Bengt Glimelius, Pia Osterlund
Summary: BRAF-V600E mutation is a negative prognostic and predictive biomarker in metastatic colorectal cancer. Non-V600E mutations (aBRAFmt) are rare and poorly understood. This study compared the mutation patterns and characteristics of aBRAFmt with other RAS/BRAF groups in three different cohorts. The study found that aBRAFmt represents a distinct subgroup with differences in clinicopathological features, and its prognosis is slightly better than BRAF-V600E but worse than RASmt and RAS & BRAFwt. aBRAFmt should not be a contraindication for metastasectomy.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Review
Chemistry, Multidisciplinary
Zhanghao Gu, Jiayi Wang, Yu Fu, Hao Pan, Hongyan He, Qi Gan, Changsheng Liu
Summary: Articular cartilage defects cause disability and global socioeconomic loss. However, the limited regeneration ability of cartilage tissue due to its avascular and nearly acellular nature poses challenges for repair and regeneration. Smart biomaterials can regulate cell behavior, provide a favorable microenvironment and induce articular cartilage repair and regeneration. The use of smart drug release systems and smart scaffolds in these processes is discussed, as well as the importance of advanced manufacturing techniques and clinical translation of smart biomaterials.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Ying-Jie Sun, Wen-Da Chen, Ji Liu, Jun-Jin Li, Yu Zhang, Wei-Qi Cai, Li Liu, Xin-Jing Tang, Jian Hou, Ming Wang, Liang Cheng
Summary: This article introduces a method for precise control of CRISPR/Cas gene editing using circular guide RNAs (gRNAs). The method involves synthesis with photo-labile substituents and allows accurate editing of specific genes in live cells and embryos.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Engineering, Environmental
Yanlei Wang, Ju Liu, Xiaoqing Yuan, Mi Wang, Yi Nie, Suojiang Zhang, Hongyan He
Summary: This Perspective discusses the structures of ionic liquids (ILs) and the aggregations that can significantly alter their micro-environment and impact their properties and functions. The mesoscale structures of IL aggregation in pure ILs and ILs-solvent mixtures are analyzed based on interacting forces and thermodynamics. The potential applications of IL aggregation in various scientific and engineering fields are explored, such as biomass conversion, smart materials, and life medicine. The challenges and opportunities in the field of IL aggregation, including direct observation techniques, large-scale simulations, theoretical forming mechanisms, and coupling mechanisms with solid interfaces, are also discussed. These new perspectives shed light on the rational design of ILs and generate research interest in ILs as a green medium in chemical engineering.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Applied
Zhiyong Li, Yuan He, Hongyan He, Weizhe Zhou, Mengru Li, Aiming Lu, Tuanjie Che, Songdong Shen
Summary: In this study, Ulva prolifera, an edible alga, was used to prepare angiotensin-I converting enzyme (ACE) inhibitory peptide. The highest ACE inhibitory activity was obtained from the hydrolysate of algae protein, which was processed using various methods and identified as the peptide DIGGL. DIGGL exhibited non-competitive inhibition against ACE and mainly achieved this effect through three conventional hydrogen bonds. It also showed the ability to activate endothelial nitric oxide synthase and decrease endothelin-1 secretion in human umbilical vein endothelial cells. Moreover, DIGGL promoted splenocyte proliferation and remained active during gastrointestinal protease digestion.
Article
Nutrition & Dietetics
Xinjing Tang, Zhirong Wang, Jiong Zheng, Jianquan Kan, Guangjing Chen, Muying Du
Summary: This study investigates the effects of steam explosion (SE) treatment on the physicochemical, structural properties, and in vitro hypoglycemic activities of adlay bran soluble dietary fiber (SDF). The results show that SE treatment significantly increases the SDF content and improves its functional properties. The SE-treated SDF exhibits higher oil-holding capacity, cholesterol adsorption capacity, glucose adsorption capacity, glucose dialysis retardation index, and alpha-amylase activity inhibition ratio compared to the SDF from untreated adlay bran.
FRONTIERS IN NUTRITION
(2023)
Article
Food Science & Technology
Wenjiang Dong, Qidi Hong, Jinhuan Cheng, Hongyan He, Yanan Li, Rongsuo Hu, Yuzhou Long
Summary: This study investigated the oxidation status of solvent-extracted and cold-pressed green coffee oil (GCO) under accelerated storage conditions using proton nuclear magnetic resonance (1H and 13C NMR) spectroscopy. Results showed that the signal intensity of oxidation products gradually increased with oxidation time, while unsaturated fatty acid signals weakened. The NMR system was found to be a fast and convenient tool for monitoring the oxidation process and quality control of GCO.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Physical
Li Cui, Yuheng Fan, Jin Kang, Caixia Yin, Weilu Ding, Hongyan He, Fangqin Cheng
Summary: In this study, a novel class of functionalized ionic liquids with grafted crown ether-moieties (CE-FILs) were reported for the efficient separation of Li-6 and Li-7. The enhanced distribution of Li+ was achieved by the synergistic effect of ion-dipole interaction, hydrogen bonding, and coordination interaction. The CE-FILs pave a new way for Li isotopes separation.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Chemistry, Physical
Shuting Xu, Yawei Liu, Xuefu Li, Bowen Yue, Feng Huo, Hongyan He, Suojiang Zhang
Summary: Molecular dynamic simulations were conducted to investigate the relationship between the microscopic structures changed by water and the ionic diffusivity in aqueous mixtures of imidazolium ionic liquids (ILs). Two distinct regimes of ionic diffusivity were identified with increasing water concentrations, which are directly correlated to the ionic association. The study also revealed two general relationships between ionic diffusivity and ion-pair lifetimes, as well as short-ranged interactions between cations and anions.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Yuqiang Hu, Changjiang Li, Minghao Hu, Zhen Zhang, Ruolan Fu, Xinjing Tang, Tongbo Wu
Summary: This article introduces a novel method of regulating the catalytic activity of DNAzyme by introducing a new domain that responds to various effector molecules. This method allows for more precise and predictable modulation of enzyme activity. Additionally, the article demonstrates the great potential of this new method in molecular computing.
Article
Biochemistry & Molecular Biology
Dao Lin, Yongqi Ke, Hongjia Chen, Yinlong Zhang, Xinjing Tang, Wei Cui, Xiangjun Li, Yujian He, Li Wu
Summary: The control of DNA assembly systems on cells is crucial for precise targeted therapies. In this study, a controllable DNA self-assembly system based on the regulation of G-quadruplex DNA topology by a reduction-sensitive azobenzene ligand is reported. The ligand can induce a complete change of G4 topology in a tissue-specific manner, allowing control of the self-assembly of G4-functionalized DNAs on cells.
Article
Chemistry, Applied
Shaofang Ma, Zihan Zhu, Jianfei Xu, Jing Wang, Xinjing Tang, Wei Bian
Summary: Novel green-emitting fluorescent carbon dots (CDs) were successfully designed and synthesized through cross amide formation of two precursors under hydrothermal treatment. The obtained CDs exhibited good photoluminescent properties with a high quantum yield. They also demonstrated good fluorescence sensitivity and selectivity for Hg2+, making them applicable for Hg2+ detection in real water samples and intracellular Hg2+ fluorescence detection.
Article
Computer Science, Information Systems
Zhangqi Zheng, Yongshan Liu, Bing Zhang, Xinqian Liu, Hongyan He, Xiang Gong
Summary: This paper proposes a multitype software buffer overflow vulnerability prediction method called MSVAGraph, which is based on the graph structure of software and a self-attentive graph neural network. It accurately predicts multiple types of software buffer overflow vulnerabilities.
INFORMATION AND SOFTWARE TECHNOLOGY
(2023)
Article
Chemistry, Physical
Wei-Lu Ding, Junwu Chen, Yumiao Lu, Guliang Liu, Bobo Cao, Chenlu Wang, Guangyong Liu, Xing-Liang Peng, Hongyan He, Suojiang Zhang
Summary: In this study, a machine learning model targeting the electronic density of Z-bonds in ionic liquids (ILs) was successfully trained. The correlations between the Z-bond energy and the electrochemical properties of IL-based systems were investigated separately. This model provides an efficient way to predict the electronic density of Z-bonds in nanoscale systems and offers insights into the electrochemical properties of ILs.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Medicine, Research & Experimental
Zhongyu Wang, Xinli Fan, Guanqun Mu, Xiaoran Zhao, Qian Wang, Jing Wang, Xinjing Tang
Summary: This study developed CB-activatable cASOs and successfully inhibited disease-related genes in tumor cells with limited effects on normal cells. Compared to linear cASOs, CB-activatable cASOs also reduced nonspecific immunostimulation.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Biochemistry & Molecular Biology
Yu Zhang, Di Feng, Guanqun Mu, Qian Wang, Jing Wang, Yun Luo, Xinjing Tang
Summary: RNA A-to-I editing is a common post-transcriptional modification in cells. By using guide RNA and exogenous ADAR enzymes, artificial intervention of A-to-I editing can be achieved at specific RNA sites. In this study, we developed photo-caged antisense guide RNA oligonucleotides with a simple cholesterol modification and successfully achieved light-triggered site-specific RNA A-to-I editing for the first time using endogenous ADAR enzymes. Our caged A-to-I editing system effectively manipulated RNA editing in living cells and 3D tumorspheres, providing a new approach for precise manipulation of gene expression and spatial regulation.
CELL CHEMICAL BIOLOGY
(2023)
