Article
Medicine, Research & Experimental
Junjun Wang, Wei Hu, Zhenyang Shen, Teng Liu, Weiming Dai, Bo Shen, Xiaoman Li, Jingni Wu, Lungen Lu, Shengli Li, Xiaobo Cai
Summary: This study established chronic liver injury mouse models and conducted single-cell RNA sequencing to characterize the transcriptional landscape of chronic liver injury, revealing different expression patterns of cells and potential liver injury markers. The findings provide insights into the underlying molecular mechanisms and potential clinical strategies for effective intervention of chronic liver diseases.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Biochemistry & Molecular Biology
Razieh Rahmati, Rasmieh Hamid, Zahra Ghorbanzadeh, Feba Jacob, Pezhman Azadi, Mehrshad Zeinalabedini, Laleh Karimi Farsad, Mehrbano Kazemi, Mohammad Ali Ebrahimi, Fahimeh Shahinnia, Ghasem Hosseini Salekdeh, Mohammad Reza Ghaffari, Mohammad Reza Hajirezaei
Summary: In this study, transcriptomics and targeted metabolic profiling were used to identify molecular changes in hydrangea plants cultured at different pH levels. The study identified potential key regulators of flower pigmentation and provided novel insights into the molecular networks in hydrangea flowers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Chunyu Zhang, Chengyu Zhang, Xiaoqiong Xu, Minzhang Liao, Ning Tong, Zihao Zhang, Yukun Chen, Xu Xu Han, Yuling Lin, Zhongxiong Lai
Summary: Our study demonstrates that the balance between various metabolic processes, pollen wall remodelling, DNA methylation, DNA repairs and the regulation of MYBs are essential for germination of wild banana pollens.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agricultural Engineering
Min Zhang, Mengting Hong, Zihao Wang, Xue Jiao, Chongde Wu
Summary: Low temperature significantly improves exopolysaccharide production in Tetragenococcus halophilus, and analysis of structure and transcriptome reveals the regulatory mechanisms of low temperature on exopolysaccharide production.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agronomy
Hong Kan, Shuai Qu, Kai Dong, Shihan Wang, Chen Xu, Yingping Wang, Shuang Hua
Summary: Due to at least 3 years of cultivation, Panax ginseng is susceptible to being attacked by pathogens and Phytophthora blight caused by Phytophthora cactorum can destroy ginseng plants. However, little research has been done on this area and its effects on the metabolic profile of ginseng are still unclear. In this study, metabolomics and transcriptomics were used to analyze the differences in health and P. cactorum-affected ginseng leaves and stems. The results showed significant changes in metabolites and genes in response to P. cactorum, providing insights into the molecular mechanism of plant-pathogen interaction.
Article
Plant Sciences
Patricia Scholz, Premysl Pejchar, Max Fernkorn, Eliska Skrabalkova, Roman Pleskot, Katharina Blersch, Teun Munnik, Martin Potocky, Till Ischebeck
Summary: Pollen tube growth requires tight regulation of pectin secretion, and molecules such as phosphoinositides and phosphatidic acid play important roles in this process. A study found that DGK5, a kinase, is localized in the plasma membrane and is involved in the regulation of pectin secretion and lipid signaling, contributing to polar tip growth in pollen tubes.
Article
Biochemistry & Molecular Biology
Zengqiang Li, Dengjie Luo, Meiqiong Tang, Shan Cao, Jiao Pan, Wenxian Zhang, Yali Hu, Jiao Yue, Zhen Huang, Ru Li, Peng Chen
Summary: Cytoplasmic male sterility (CMS) is widely used in hybrid seed production. This study investigated the physiological, DNA methylation, and transcriptome differences between a kenaf CMS line and its maintainer line. The results revealed significant differences in hormone levels, photosynthetic products, and ATP contents between the CMS and maintainer lines. The analysis also identified differentially methylated genes and differentially expressed genes that play important roles in carbon metabolism, plant hormone signal transduction, and other pathways. This study provides new insights into the epigenetic mechanism of CMS in kenaf and other crops.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Zhengrong Hu, Weijie Yan, Chenkai Yang, Xuebing Huang, Xutong Hu, Yangyang Li, Jiashuo Yang, Shipeng Xang, Pengfei Yi, Risheng Hu
Summary: This study reveals the molecular mechanisms of tobacco cold response through integrated biochemical, transcriptomic, and metabolomic analyses. The two tobacco cultivars had similar mechanisms in response to long-term cold stress, while more enriched pathways were identified in the cold-sensitive cultivar under short-term stress, mainly related to amino acid metabolism. By analyzing the correlation between metabolite contents and gene expression modules, 12 key candidate genes were identified, which are involved in tobacco cold response and recovery.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Monica Scali, Alessandra Moscatelli, Luca Bini, Elisabetta Onelli, Rita Vignani, Wei Wang
Summary: The elongation of pollen tubes relies on a vesicular transport system and actin cytoskeleton, making them an ideal model system for studying various cellular processes and signaling network. Proteomic analysis of Nicotiana tabacum Differentially Abundant Proteins (DAPs) after treatment with membrane trafficking inhibitors revealed proteins related to energy metabolism, structural features, stress response, and protein degradation. In-depth analysis of energy-related pathways suggests that the male gametophyte serves as a reliable model for energy reservoir and dynamics.
Article
Developmental Biology
Katarina Kulichova, Vinod Kumar, Lenka Steinbachova, Bozena Klodova, Ljudmilla Timofejeva, Miloslav Juricek, David Honys, Said S. Hafidh
Article
Biochemistry & Molecular Biology
Anna J. Wiese, Lenka Steinbachova, Ljudmilla Timofejeva, Vojtech Cermak, Bozena Klodova, Ranjani S. Ganji, Mariana Limones-Mendez, Pavel Bokvaj, Said Hafidh, David Potesil, David Honys
Summary: The study reveals that bZIP18 and bZIP52 are sequestered to the cytoplasm under control conditions but re-localized to the nuclei under heat stress, where they jointly regulate gene expression. The interaction of these transcription factors with 14-3-3 epsilon plays a crucial role in their subcellular localization and function in response to heat stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Maria Flores-Tornero, Lele Wang, David Potesil, Said Hafidh, Frank Vogler, Zbynek Zdrahal, David Honys, Stefanie Sprunck, Thomas Dresselhaus
Summary: Analyses of secretomes from in vitro grown pollen tubes reveal significant differences in protein composition and pathways among different plant species, particularly in relation to functions associated with cell wall, cell surface, energy metabolism, lipid metabolism, proteolysis, and redox processes. Novel small secreted proteins were identified, and it was found that secreted proteins containing pro-peptides are processed in the apoplast.
PLANT REPRODUCTION
(2021)
Review
Plant Sciences
Said Hafidh, David Honys
Summary: The gametophyte represents the sexual phase in the alternation of generations in plants, while the sporophyte represents the nonsexual phase. This paper reviews the evolutionary origins of the male gametophyte among land plants, particularly its ontogenesis in flowering plants, highlighting recent advances in regulatory mechanisms involved in pollen development.
ANNUAL REVIEW OF PLANT BIOLOGY, VOL 72, 2021
(2021)
Review
Plant Sciences
Palak Chaturvedi, Anna J. Wiese, Arindam Ghatak, Lenka Zaveska Drabkova, Wolfram Weckwerth, David Honys
Summary: Plants face the challenge of responding to unfavorable local conditions such as heat stress, which contributes to global crop losses. It is crucial to develop crops that are tolerant to heat stress and other abiotic stresses to ensure global food security. This review focuses on how pollen is affected by heat stress and the molecular mechanisms involved in the stress response.
Article
Biochemistry & Molecular Biology
Petr Fajkus, Agata Kilar, Andrew D. L. Nelson, Marcela Hola, Vratislav Peska, Ivana Goffova, Miloslava Fojtova, Dagmar Zachova, Jana Fulneckova, Jiri Fajkus
Summary: The study characterized the evolution of telomerase RNA (TR) in early diverging Viridiplantae and other Diaphoretickes lineages through identifying conserved upstream sequence elements and TR template regions. The results demonstrated deep conservation of TR motifs across different species, highlighting the common origin and evolutionary transitions in telomere repeats.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Plant Sciences
Katarina Kulichova, Janto Pieters, Vinod Kumar, David Honys, Said Hafidh
Summary: In this study, the essential role of AKRP protein in plastid differentiation and morphogenesis in Arabidopsis was revealed. Loss of AKRP function resulted in embryo developmental arrest and affected the viability and pollen tube attraction of gametophytes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Bozena Klodova, David Potesil, Lenka Steinbachova, Christos Michailidis, Ann-Cathrin Lindner, Dieter Hackenberg, Jorg D. Becker, Zbynek Zdrahal, David Twell, David Honys
Summary: This study provides a comprehensive investigation into the gene expression dynamics in developing Arabidopsis pollen by integrating RNA and proteomic data. The findings identify potential regulatory factors, changes in alternative splicing, and provide insights into mRNA translation rate and storage, laying the foundation for further studies on the role of alternative splicing in this model.
PLANT REPRODUCTION
(2022)
Article
Plant Sciences
Eva Dvorak Tomastikova, Fen Yang, Kristina Mlynarova, Said Hafidh, Sarka Schorova, Alzbeta Kusova, Marketa Pernisova, Tereza Prerovska, Bozena Klodova, David Honys, Jiri Fajkus, Ales Pecinka, Petra Prochazkova Schrumpfova
Summary: RUVBL1 and RUVBL2 are crucial for the proper development of male and female gametophytes in Arabidopsis. Mutations in these genes result in abnormal embryo sac structure and nuclear numbers. The expression of these proteins is observed in pollen grains, embryo sac, and tapetum cells, playing important roles in reproductive development.
Correction
Plant Sciences
Bozena Klodova, David Potesil, Lenka Steinbachova, Christos Michailidis, Ann-Cathrin Lindner, Dieter Hackenberg, Jorg D. Becker, Zbynek Zdrahal, David Twell, David Honys
PLANT REPRODUCTION
(2023)
Article
Plant Sciences
Irene Julca, Camilla Ferrari, Maria Flores-Tornero, Sebastian Proost, Ann-Cathrin Lindner, Dieter Hackenberg, Lenka Steinbachova, Christos Michaelidis, Sonia Gomes Pereira, Chandra Shekhar Misra, Tomokazu Kawashima, Michael Borg, Frederic Berger, Jacob Goldberg, Mark Johnson, David Honys, David Twell, Stefanie Sprunck, Thomas Dresselhaus, Joerg D. Becker, Marek Mutwil
Summary: The study found that organ-specific gene families predate the origins of the organs themselves, and the appearance of plant organs drove the rapid evolution of land plants. The evolution of new organs mainly involves the co-option of existing genes. Male gametes have a high number and conservation of specific genes, indicating a high degree of specialization.