Article
Biochemistry & Molecular Biology
Yasir Sidiq, Masataka Nakano, Yumi Mori, Takashi Yaeno, Makoto Kimura, Takumi Nishiuchi
Summary: Nicotinamide pretreatment effectively suppresses Fusarium Head Blight in wheat plants and significantly reduces DON mycotoxin accumulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Jianhua Wang, Shanhai Lin, Guanghui Zhu, Zhiyong Zhao, Haoyu Wang, Miaoping Zhou, Xingming Zhao, Aibo Wu
Summary: This study reveals the molecular mechanisms of trichothecene biosynthesis in the Fusarium graminearum species complex under non-inducing conditions through transcriptome and proteome analysis. Differential gene expression and protein analysis of different strains identified novel genes and proteins associated with trichothecene biosynthesis.
Article
Plant Sciences
Fei Xu, Wei Liu, Yuli Song, Yilin Zhou, Xiangming Xu, Gongqiang Yang, Junmei Wang, Jiaojiao Zhang, Lulu Liu
Summary: The study in the main wheat production area of China found that climate factors, rather than cropping systems, had a greater impact on the relative prevalence of Fusarium graminearum and Fusarium asiaticum. Environmental conditions during the overwintering period appeared to be more important than those during other growth stages.
Article
Biotechnology & Applied Microbiology
Laurie Taylor, Santiago Gutierrez, Susan P. McCormick, Matthew G. Bakker, Robert H. Proctor, Jennifer Teresi, Ben Kurtzman, Guixia Hao, Martha M. Vaughan
Summary: By emitting the signaling molecule trichodiene (TD), transgenic Trichoderma has enhanced biocontrol activity against Fusarium, reducing DON contamination. TD can also influence the expression of pathogenesis-related genes and has different effects on different wheat cultivars.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Agronomy
Vesna Zupunski, Radivoje Jevtic, Mirjana Lalosevic, Sanja Mikic, Branka Orbovic
Summary: In this study, species- and trichothecene-specific primers were used to monitor the F. graminearum species complex (FGSC) originating from Serbia. The primers were not specific for 11% of the F. graminearum population, but were effective in multilocus genotyping of Tri7, Tri3, and Tri5 genes. Alternaria spp. had a significant influence on grain infection with F. graminearum, indicating the need for further investigation.
Article
Food Science & Technology
Asmaa Abbas, Tapani Yli-Mattila
Summary: This study demonstrates that treatment with the methanolic extract of Zanthoxylum bungeanum can reduce the growth of F. graminearum and inhibit the production of deoxynivalenol. The extract is mainly composed of four flavonoids. In vitro bioassays show that the extract can inhibit fungal growth. After biocontrol treatment, the levels of F. graminearum DNA and deoxynivalenol were reduced.
Article
Biochemistry & Molecular Biology
Orsolya Kinga Gondor, Judit Tajti, Kamiran Aron Hamow, Imre Majlath, Gabriella Szalai, Tibor Janda, Magda Pal
Summary: This study aimed to investigate the influence of polyamine metabolism under different light conditions. It was found that polyamines had varying effects on photosynthesis under different light regimes, suggesting a complex relationship between polyamines and plant adaptation to light conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Nutrition & Dietetics
Kun Luo, Therese Ouellet, Huiyan Zhao, Xiukang Wang, Zhensheng Kang
Summary: Recent studies have shown synergistic effects of the coexistence of the English grain aphid Sitobion avenae and phytopathogen Fusarium graminearum on wheat spikes. Understanding the relationship between wheat-aphids and wheat-pathogens has contributed to the understanding of these tripartite interactions at the ecological level. In addition, preexposure to the herbivore S. avenae triggers the accumulation of mycotoxins, stimulating the infection process of F. graminearum and the epidemic of Fusarium head blight (FHB) in agroecosystems.
FRONTIERS IN NUTRITION
(2021)
Article
Agronomy
Sebastian Michel, Barbara Steiner, Hermann Buerstmayr
Summary: Fusarium head blight (FHB) is a destructive wheat disease causing yield losses and mycotoxin contamination in crop. Study shows strong correlation between resistance against multiple Fusarium species, mycotoxin contents, FHB severity and FHB-associated traits. Indirect phenotypic and genomic selection, using low anther retention and evaluating pre-selected genotypes in disease nurseries, is suggested as a suitable strategy for breeding wheat cultivars with reduced risk of mycotoxin accumulation.
Article
Plant Sciences
Zhen Yan, Wanquan Chen, Theo van der Lee, Cees Waalwijk, Anne D. van Diepeningen, Jie Feng, Hao Zhang, Taiguo Liu
Summary: Exploiting wheat cultivars with stable resistance to Fusarium Head blight and toxin accumulation is an effective strategy to reduce yield losses and mycotoxin contamination. Investigation in China's major wheat growing regions revealed that growing region and cultivar significantly affect the occurrence of FHB and mycotoxin accumulation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Matthew G. Bakker, Briana K. Whitaker, Susan P. McCormick, Elizabeth A. Ainsworth, Martha M. Vaughan
Summary: This study investigated the microbiomes associated with wheat plants under different atmospheric carbon dioxide concentration levels and found that changing atmospheric compositions can affect the dynamics of plant-associated microbiomes. The abundance of certain sequence variants differed significantly in response to carbon dioxide levels, and fungal pathogens also responded differently to these changes.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agronomy
Xiaojing Yan, Ming Wang, Yuxiao Zhu, Xin Shi, Xiaohui Liu, Yixuan Chen, Jun Xu, Daibin Yang, Huizhu Yuan
Summary: The addition of aviation spray adjuvant not only ensures high control effect of prothioconazole on Fusarium head blight in wheat and increases wheat yield, but also greatly reduces the content of DON mycotoxin, ensuring the safety of wheat production.
Article
Agriculture, Multidisciplinary
Dimitrios Drakopoulos, Andreas Kaegi, Johan Six, Alexander Zorn, Felix E. Wettstein, Thomas D. Bucheli, Hans-Rudolf Forrer, Susanne Vogelgsang
Summary: Intercropping or cover cropping with certain plant species before the wheat growing season under reduced tillage practices can reduce mycotoxin contamination in wheat while maintaining crop yield. The use of specific intercrops or cover crops, such as white mustard, Indian mustard, or winter pea, significantly decreased deoxynivalenol levels in wheat and improved yield. While these innovative cropping systems showed promising results in reducing mycotoxins, economic trade-offs were observed due to increased operating costs.
AGRICULTURAL SYSTEMS
(2021)
Article
Microbiology
Bimal Paudel, Connor Pedersen, Yang Yen, Shin-Yi Lee Marzano
Summary: Research identified that DON toxin production by F. graminearum during infection of small grain crops is a major contributor to economic losses. A novel strain of Fusarium graminearum virus 1 (FgV1-SD4) was discovered, which showed reduced virulence of F. graminearum against wheat and decreased DON accumulation in infected seeds. This mycovirus strain and specific genetic components of FgV1-SD4 could be further optimized as a biocontrol agent to reduce Fusarium head blight and DON accumulation in small grain crops.
Article
Biochemistry & Molecular Biology
Magda Pal, Kamiran Aron Hamow, Altafur Rahman, Imre Majlath, Judit Tajti, Orsolya Kinga Gondor, Mohamed Ahres, Fatemeh Gholizadeh, Gabriella Szalai, Tibor Janda
Summary: This study compared the effects of different spectral compositions on polyamine metabolism in plants and found that light quality can optimize plant growth by adjusting polyamine metabolism at the gene expression level.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Mohammed Khudhair, F. Obanor, K. Kazan, D. M. Gardiner, E. Aitken, A. McKay, D. Giblot-Ducray, S. Simpfendorfer, L. F. Thatcher
Summary: This study investigated the genetic diversity of Fusarium pseudograminearum populations in different regions of Australia, revealing differences in mating type composition between Eastern states and Western Australia, while all isolates across regions shared the same chemotype. Genetic markers showed high genotypic variation within and between populations, indicating possible sexual reproduction in the pathogen in certain locations. These findings enhance understanding of Fp population dynamics in Australia and emphasize the importance of monitoring for potential shifts in the pathogen population for effective disease management.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Plant Sciences
David M. Geiser, Abdullah M. S. Al-Hatmi, Takayuki Aoki, Tsutomu Arie, Virgilio Balmas, Irene Barnes, Gary C. Bergstrom, Madan K. Bhattacharyya, Cheryl L. Blomquist, Robert L. Bowden, Balazs Brankovics, Daren W. Brown, Lester W. Burgess, Kathryn Bushley, Mark Busman, Jose F. Cano-Lira, Joseph D. Carrillo, Hao-Xun Chang, Chi-Yu Chen, Wanquan Chen, Martin Chilvers, Sofia Chulze, Jeffrey J. Coleman, Christina A. Cuomo, Z. Wilhelm de Beer, G. Sybren de Hoog, Johanna Del Castillo-Munera, Emerson M. Del Ponte, Javier Dieguez-Uribeondo, Antonio Di Pietro, Veronique Edel-Hermann, Wade H. Elmer, Lynn Epstein, Akif Eskalen, Maria Carmela Esposto, Kathryne L. Everts, Sylvia P. Fernandez-Pavia, Gilvan Ferreira da Silva, Nora A. Foroud, Gerda Fourie, Rasmus J. N. Frandsen, Stanley Freeman, Michael Freitag, Omer Frenkel, Kevin K. Fuller, Tatiana Gagkaeva, Donald M. Gardiner, Anthony E. Glenn, Scott E. Gold, Thomas R. Gordon, Nancy F. Gregory, Marieka Gryzenhout, Josep Guarro, Beth K. Gugino, Santiago Gutierrez, Kim E. Hammond-Kosack, Linda J. Harris, Monika Homa, Cheng-Fang Hong, Laszlo Hornok, Jenn-Wen Huang, Macit Ilkit, Adriaana Jacobs, Karin Jacobs, Cong Jiang, Maria Del Mar Jimenez-Gasco, Seogchan Kang, Matthew T. Kasson, Kemal Kazan, John C. Kennell, Hye-Seon Kim, H. Corby Kistler, Gretchen A. Kuldau, Tomasz Kulik, Oliver Kurzai, Imane Laraba, Matthew H. Laurence, Theresa Lee, Yin-Won Lee, Yong-Hwan Lee, John F. Leslie, Edward C. Y. Liew, Lily W. Lofton, Antonio F. Logrieco, Manuel S. Lopez-Berges, Alicia G. Luque, Erik Lysoe, Li-Jun Ma, Robert E. Marra, Frank N. Martin, Sara R. May, Susan P. McCormick, Chyanna McGee, Jacques F. Meis, Quirico Migheli, N. M. I. Mohamed Nor, Michel Monod, Antonio Moretti, Diane Mostert, Giuseppina Mule, Francoise Munaut, Gary P. Munkvold, Paul Nicholson, Marcio Nucci, Kerry O'Donnell, Matias Pasquali, Ludwig H. Pfenning, Anna Prigitano, Robert H. Proctor, Stephane Ranque, Stephen A. Rehner, Martijn Rep, Gerardo Rodriguez-Alvarado, Lindy Joy Rose, Mitchell G. Roth, Carmen Ruiz-Roldan, Amgad A. Saleh, Baharuddin Salleh, Hyunkyu Sang, Maria Mercedes Scandiani, Jonathan Scauflaire, David G. Schmale, Dylan P. G. Short, Adnan Sisic, Jason A. Smith, Christopher W. Smyth, Hokyoung Son, Ellie Spahr, Jason E. Stajich, Emma Steenkamp, Christian Steinberg, Rajagopal Subramaniam, Haruhisa Suga, Brett A. Summerell, Antonella Susca, Cassandra L. Swett, Christopher Toomajian, Terry J. Torres-Cruz, Anna M. Tortorano, Martin Urban, Lisa J. Vaillancourt, Gary E. Vallad, Theo A. J. van der Lee, Dan Vanderpool, Anne D. van Diepeningen, Martha M. Vaughan, Eduard Venter, Marcele Vermeulen, Paul E. Verweij, Altus Viljoen, Cees Waalwijk, Emma C. Wallace, Grit Walther, Jie Wang, Todd J. Ward, Brian L. Wickes, Nathan P. Wiederhold, Michael J. Wingfield, Ana K. M. Wood, Jin-Rong Xu, Xiao-Bing Yang, Tapani Yli-Mattila, Sung-Hwan Yun, Latiffah Zakaria, Hao Zhang, Ning Zhang, Sean X. Zhang, Xue Zhang
Summary: The debate over the classification of the Fusarium genus in recent years has focused on whether the FSSC should be included in Fusarium, but molecular phylogenetic research has shown strong support for the monophyly of Fusarium including the FSSC, which is considered the best taxonomic option available.
Article
Mycology
Donald M. Gardiner, Anca Rusu, Aurelie H. Benfield, Kemal Kazan
Summary: A key gene, velvet A, regulating virulence in Fusarium pseudograminearum was identified, and knockout mutants of velvet A showed non-pathogenicity towards wheat. The mutation was only found in a single field isolate and its origin remains unknown.
Article
Mycology
Kylie R. Chambers, Angela P. Van de Wouw, Donald M. Gardiner, Candace E. Elliott, Alexander Idnurm
Summary: The study found that continuous passaging in vitro can result in accumulation of changes in DNA sequence in fungi, affecting their properties and leading to differences from the original isolates. A base pair substitution that occurred during in vitro passaging contributed to attenuation of pathogenicity in Leptosphaeria maculans. Mutation in the gene encoding a putative Zn2(II)Cys6 transcription factor co-segregated with reduction in pathogenicity, indicating a potential genetic basis for the altered pathogenicity.
Article
Cell Biology
Francesco Tini, Giovanni Beccari, Gianpiero Marconi, Andrea Porceddu, Micheal Sulyok, Donald M. Gardiner, Emidio Albertini, Lorenzo Covarelli
Summary: The study investigated how Fusarium graminearum regulates virulence towards its natural hosts through DNA methylation. It was found that sub-culturing led to a decline in virulence, which could be restored through repeated inoculation. DNA methylation analysis revealed changes in methylation levels of genes related to virulence after subculturing, suggesting the potential of ddRAD-MCSeEd approach in detecting genes associated with fungal virulence.
Article
Biochemistry & Molecular Biology
Erin E. Hahn, Marina R. Alexander, Alicia Grealy, Jiri Stiller, Donald M. Gardiner, Clare E. Holleley
Summary: This study successfully recovered complete mitochondrial genomes and up to 3x nuclear genome coverage from formalin-preserved tissues by refining sample processing methods and identifying specimen characteristics predictive of sequencing success. The overall specimen condition was found to be the strongest predictor of DNA yield, interacting with preservation conditions to accelerate DNA degradation.
MOLECULAR ECOLOGY RESOURCES
(2021)
Article
Biochemical Research Methods
Rosalie Sabburg, Aphrika Gregson, Andrew S. Urquhart, Elizabeth A. B. Aitken, Linda Smith, Louise F. Thatcher, Donald M. Gardiner
Summary: The method presented in the study allows for accurate evaluation of different fungi, especially those that can be inoculated with spores. It can assess susceptibility or resistance to antifungal drugs in a short period of time, taking into account inherent growth differences between strains.
JOURNAL OF MICROBIOLOGICAL METHODS
(2021)
Article
Biotechnology & Applied Microbiology
Tobias Bruun Pedersen, Mikkel Rank Nielsen, Sebastian Birkedal Kristensen, Eva Mie Lang Spedtsberg, Trine Sorensen, Celine Petersen, Jens Muff, Teis Esben Sondergaard, Kare Lehmann Nielsen, Reinhard Wimmer, Donald Max Gardiner, Jens Laurids Sorensen
Summary: The biosynthetic pathways for fungal polyketides have been reconstructed and expressed heterologously in yeast. The study found that FvPPT1 from Fusarium verticillioides showed the highest efficiency in producing the target polyketides. The results indicate that selecting an optimal PPTase partner can enhance the production of target polyketides.
MICROBIAL CELL FACTORIES
(2022)
Article
Plant Sciences
Yi Ding, Donald M. Gardiner, Jonathan J. Powell, Michelle L. Colgrave, Robert F. Park, Kemal Kazan
Summary: This study investigated the transcriptional and metabolic responses in Brachypodium distachyon roots following challenge with Fusarium graminearum. It was found that Bd roots recognized encroaching Fg prior to physical contact by altering gene expression and secretion of host metabolites, resulting in increased resistance to the pathogen.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Microbiology
Yi Ding, Donald M. Gardiner, Kemal Kazan
Summary: This study analyzed the transcriptome of the fungal pathogen Fusarium graminearum (Fg) during root infection of model cereal Brachypodium distachyon (Bd). The study revealed both shared and unique infection strategies employed by Fg during colonization of different host tissues. The role of deoxynivalenol (DON), a well-known virulence factor, during root infection was also explored.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Jonatan Nino-Sanchez, Prabhakaran T. Sambasivam, Anne Sawyer, Rachael Hamby, Angela Chen, Elizabeth Czislowski, Peng Li, Narelle Manzie, Donald M. Gardiner, Rebecca Ford, Zhi Ping Xu, Neena Mitter, Hailing Jin
Summary: Spray-induced gene silencing (SIGS) is a promising tool for controlling fungal plant diseases. Delivering dsRNA as BioClay can enhance RNA durability on plants and prolong its protection against fungal pathogens. This research represents a major step forward for the adoption of SIGS as an eco-friendly alternative to traditional fungicides.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Plant Sciences
Rebecca M. Degnan, Alistair R. McTaggart, Louise S. Shuey, Leny Jane S. Pame, Grant R. Smith, Donald M. Gardiner, Volker Nock, Rebecca Soffe, Sarah Sale, Ashley Garrill, Bernard J. Carroll, Neena Mitter, Anne Sawyer
Summary: Rust fungi are a diverse group of plant pathogens that pose threats to the diversity of native flora and cause crop yield losses. RNA interference induced by exogenous dsRNA has potential for managing rust fungi. Targeting key genes with exogenous dsRNA significantly reduces germination and development of infection structures in rust fungi, and has broad applicability.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Microbiology
Andrew S. Urquhart, Donald M. Gardiner
Summary: Fungal spore killers are genetic elements that promote their own inheritance by killing non-inheriting gametes. However, little is known about how they kill other spores within the fungal ascus. In this study, we investigate the spore killer protein SPOK1 in Podospora anserina and demonstrate that it kills by targeting DNA. This finding provides insights into the mechanisms of spore killers and their potential applications.
Article
Multidisciplinary Sciences
Andrew S. Urquhart, Aaron A. Vogan, Donald M. Gardiner, Alexander Idnurm
Summary: Transposable elements in eukaryotes, known as Starships in fungal genomes, have been found to confer beneficial traits to their hosts and can jump between species. They are vulnerable to defense mechanisms against mobile elements, affecting their evolutionary stability.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Biochemistry & Molecular Biology
Christopher W. G. Mann, Anne Sawyer, Donald M. Gardiner, Neena Mitter, Bernard J. Carroll, Andrew L. Eamens
Summary: The conflict between the need to conserve natural ecosystems and to feed an expanding population is driving the demand for more ecologically sustainable pathogen control measures. RNA-based biopesticides offer an eco-friendly alternative to conventional fungicides for crop protection. The development of pathogen-specific RNAi-inducing sprays and the understanding of gene silencing pathways in plants and fungi are crucial for the success of this technology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)