Article
Multidisciplinary Sciences
Hideaki Kai, Kenta Teruya, Atsuko Takeuchi, Yoshikazu Nakamura, Hidehiro Mizusawa, Masahito Yamada, Tetsuyuki Kitamoto
Summary: The polymorphic heterozygosity of PRNP at codon 129 or 219 prevents the onset of sporadic Creutzfeldt-Jakob disease (sCJD). This study investigated the association between polymorphic geno-types at codon 129 or 219 and comprehensive prion disease onset using non-CJD as a reference. Results showed that EK heterozygotes at codon 219 had a preventive effect on certain prion diseases but not others, suggesting a possible mechanism involving electrostatic interactions between PrP molecules.
Article
Virology
Simone Baiardi, Angela Mammana, Marcello Rossi, Anna Ladogana, Benedetta Carla, Pierluigi Gambetti, Sabina Capellari, Piero Parchi
Summary: Variably protease-sensitive prionopathy is a rare and underestimated prion disease with diverse and often non-specific clinical and pathological features. The host genetic background, particularly the PRNP codon 129 genotype, plays a significant role in phenotypic modulation. The sensitivity of pathological prion protein to proteinase K digestion varies based on different genotypes, leading to distinct PrPSc profiles and cerebral/cerebellar PrP mini-plaque patterns.
Article
Clinical Neurology
Akiko Uchino, Yuko Saito, Saori Oonuma, Shigeo Murayama, Saburo Yagishita, Tetsuyuki Kitamoto, Kazuko Hasegawa
Summary: This article reports a case of atypical VPSPr with memory loss without motor symptoms. DWI showed hyperintensity in the cerebral cortex and EEG showed nonspecific change.
Article
Microbiology
Weiguanliu Zhang, Xiangzhu Xiao, Mingxuan Ding, Jue Yuan, Aaron Foutz, Mohammed Moudjou, Tetsuyuki Kitamoto, Jan P. M. Langeveld, Li Cui, Wen-Quan Zou
Summary: This study reveals the formation process of ladder-like PrPSc in variably protease-sensitive prionopathy (VPSPr), emphasizing its association with basic pH conditions. The research findings suggest that the glycoform-selective PrPSc in VPSPr is characterized by altered glycosylation and exhibits lower aggregation seeding activity compared to PrPSc in sporadic CJD (sCJD).
Article
Behavioral Sciences
Juebin Huang, Mark Cohen, Jiri Safar, Alexander P. Auchus
Summary: This case report highlights the clinical features of a 46-year-old male patient with VPSPr, emphasizing the importance of considering VPSPr in the spectrum of prion disease when evaluating individuals with rapidly progressive dementia.
COGNITIVE AND BEHAVIORAL NEUROLOGY
(2021)
Article
Infectious Diseases
Jesus Ruiz-Cabello, Iker A. Sevilla, Ekine Olaizola, Javier Bezos, Ana B. Miguel-Coello, Marta Munoz-Mendoza, Marta Beraza, Joseba M. Garrido, Jose L. Izquierdo-Garcia
Summary: Plasma metabolic TB profile was identified using high-field nuclear magnetic resonance (NMR) spectroscopy, and this characteristic profile was successfully measured using low-field benchtop (LF) NMR as an affordable molecular technology for TB diagnosis. The study showed that metabolic fingerprinting using HF and LF-NMR could differentiate TB subjects from uninfected animals, and PTB and PTB-vaccinated subjects who may show false positive results for TB.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Immunology
Maria Canive, Gerard Badia-Bringue, Patricia Vazquez, Joseba M. Garrido, Ramon A. Juste, Almudena Fernandez, Oscar Gonzalez-Recio, Marta Alonso-Hearn
Summary: Although the genetic susceptibility to diseases has been extensively studied, the genetic loci and mechanisms that control disease tolerance are still largely unknown. In this study, the associations between host genetics and paratuberculosis (PTB) tolerance in cattle were investigated. The researchers found several single nucleotide polymorphisms associated with PTB tolerance, shedding light on the mechanisms underlying this trait.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Veterinary Sciences
Cristina Blanco-Vazquez, Marta Alonso-Hearn, Natalia Iglesias, Patricia Vazquez, Ramon A. Juste, Joseba M. Garrido, Ana Balseiro, Maria Canive, Javier Amado, Manuel A. Queipo, Tania Iglesias, Rosa Casais
Summary: Bovine paratuberculosis is a chronic enteritis caused by Mycobacterium avium subspecies paratuberculosis, which has a significant economic impact. Current diagnostic methods are unable to detect subclinically infected animals, but a study found that an ABCA13-based ELISA can improve diagnostic accuracy.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Tamara Kleinbielen, Felix Olasagasti, Daniel Azcarate, Elena Beristain, Amparo Viguri-Diaz, Isabel Guerra-Merino, Africa Garcia-Orad, Marian M. de Pancorbo
Summary: Research on m(6)A-associated SNPs in breast cancer has become increasingly important. In silico analysis identified 981 m(6)A-SNPs related to breast cancer, with four showing eQTL effects. Experimental gene expression analysis revealed only one m(6)A-SNP with differences in breast cancer tissues.
Article
Infectious Diseases
Lucia Varela-Castro, Marta Barral, Maria Cruz Arnal, Daniel Fernandez de Luco, Christian Gortazar, Joseba M. Garrido, Iker A. Sevilla
Summary: This study found that there is a high diversity of non-tuberculous mycobacteria (NTM) circulating among wildlife and livestock in Spain. Wild boar and M. avium seem to play a significant role in this epidemiological scenario.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Immunology
Paul D. Kasaija, Marinela Contreras, Fredrick Kabi, Swidiq Mugerwa, Joseba M. Garrido, Christian Gortazar, Jose de la Fuente
Summary: Tick vaccines are crucial for the control of tick infestations and tickborne diseases. A recent study demonstrated the effectiveness of oral vaccination combining Rhipicephalus microplus SUB with heat inactivated Mycobacterium bovis (IV) for the control of cattle tick infestations. In this study, a controlled pen trial was conducted to evaluate the efficacy of an oral formulation combining R. decoloratus SUB with IV for the control of R. decoloratus and Rhipicephalus appendiculatus cattle tick infestations. The results showed that the SUB + IV formulation effectively controlled tick infestations through the activation of multiple immune mechanisms.
Article
Infectious Diseases
Nuno Santos, Elisa Ferreras Colino, Maria Cruz Arnal, Daniel Fernandez de Luco, Iker Sevilla, Joseba M. Garrido, Eliana Fonseca, Ana M. Valente, Ana Balseiro, Joao Queiros, Virgilio Almeida, Joaquin Vicente, Christian Gortazar, Paulo Celio Alves
Summary: The study quantified the contribution of wild boar and red deer to the maintenance of animal tuberculosis in multi-host communities. It found that the wild boar is the main maintenance host in most sites, while the prevalence of TB in red deer likely drives the epidemiology in multi-host communities.
Article
Multidisciplinary Sciences
Cristian Melgarejo, Carles Planas, Alex Cobos, Claudia Arrieta-Villegas, Iker A. Sevilla, Javier Bezos, Xavier Moll, Yvonne Espada, Joseba M. Garrido, Mariano Domingo, Enric Vidal, Bernat Perez de Val
Summary: This study assessed the efficacy of a heat-inactivated Mycobacterium caprae (HIMC) vaccine in goats challenged with the same strain of M. caprae. The findings showed that vaccinated goats had reduced lung lesions and bacterial load compared to unvaccinated controls, indicating enhanced protection against M. caprae challenge.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Belen Navarro-Lopez, Miriam Baeta, Eva Granizo-Rodriguez, O. Moreno-Lopez, Tamara Kleinbielen, Joana Francesca Ferragut, Antonia Picornell, Marian M. de Pancorbo
Summary: Y chromosomal short tandem repeats (Y-STRs) are a useful complementary tool in forensic investigations. A novel multiplex panel has been developed to efficiently update Y-STR haplotypes, offering high sensitivity, tolerance to inhibitors, and applicability to degraded DNA samples and male mixtures.
SCIENTIFIC REPORTS
(2022)
Article
Nutrition & Dietetics
Silvia Martin-Fernandez-de-Labastida, Iker Alegria-Lertxundi, Marian M. de Pancorbo, Marta Arroyo-Izaga
Summary: This observational study found an association between intake of B group vitamins, methionine, total choline, and betaine and colorectal cancer risk. After adjusting for folate, high intake of choline and betaine remained associated with a reduced risk of colorectal cancer. The data suggest that choline and betaine intake influence colorectal cancer risk in the studied population.
EUROPEAN JOURNAL OF NUTRITION
(2023)
Article
Microbiology
Gerard Badia-Bringue, Maria Canive, Patricia Vazquez, Joseba M. Garrido, Almudena Fernandez, Ramon A. Juste, Jose Antonio Jimenez, Oscar Gonzalez-Recio, Marta Alonso-Hearn
Summary: This study suggests that cows with higher levels of interferon-gamma (IFN-γ) may have better control of Mycobacterium avium subsp. paratuberculosis (MAP) infection. By measuring IFN-γ production in blood samples of 152 Holstein cattle, a specific genetic profile associated with high IFN-γ response was identified. These findings provide important insights into the host resistance mechanisms against MAP infection.
Article
Multidisciplinary Sciences
Ramon A. Juste, Cristina Blanco-Vazquez, Marta Barral, Jose Miguel Prieto, Lucia Varela-Castro, Sandrine Lesellier, Dipesh Dave, Iker A. Sevilla, Ana Belen Martin Ezquerra, Hans Adriaensen, Gloria Herrero-Garcia, Joseba M. Garrido, Rosa Casais, Ana Balseiro
Summary: Badgers are a prominent reservoir for tuberculosis in Europe, and a study found that delivering an oral tuberculosis vaccine via food bait can partially protect wild badger populations. However, the efficacy of the vaccine may vary depending on the immune status and overall condition of the badgers at the time of vaccination. Further optimization of the vaccine strategy is necessary to reduce tolerization.
Article
Multidisciplinary Sciences
Leire Fernandez-Veiga, Miguel Fuertes, Maria V. Geijo, Bernat Perez de Val, Enric Vidal, Lorraine Michelet, Maria Laura Boschiroli, Alberto Gomez-Buendia, Javier Bezos, Gareth J. Jones, Martin Vordermeier, Ramon A. Juste, Joseba M. Garrido, Iker A. Sevilla
Summary: The single and comparative intradermal tuberculin tests (SITT and CITT) are official tests for bovine tuberculosis (TB) diagnosis using bovine and avian purified protein derivatives (PPD-B and PPD-A). However, these tests may result in nonspecific reactions due to infection with bacteria other than Mycobacterium tuberculosis complex (MTC). In this study, the performance of the skin test with PPDs and new defined antigens was evaluated in the guinea pig model. The results suggest that complementing or replacing PPD-B could improve specificity without losing sensitivity.
SCIENTIFIC REPORTS
(2023)
Article
Agriculture, Multidisciplinary
Maria X. Cuerda, Maria A. Colombatti, Maria J. Gravisaco, Maria J. Marfil, Soledad Barandiaran, Iker A. Sevilla, Joseba M. Garrido, Roberto D. Moyano, Martin J. Zumarraga, Maria Romano, Ramon A. Juste, Maria de la Paz Santangelo
Summary: This study investigates the pathological and immunological consequences of M. bovis infection in previous sensitized animals. The results suggest that oral infection after sensitization may exacerbate the lesions caused by infection and increase the humoral immune response.
SPANISH JOURNAL OF AGRICULTURAL RESEARCH
(2022)