Article
Plant Sciences
Rohit Bharati, Madhab Kumar Sen, Ram Kumar, Aayushi Gupta, Jana Ziarovska, Eloy Fernandez-Cusimamani, Olga Leuner
Summary: This study assessed the suitability of ten commonly used reference genes as optimal reference genes in M. officinalis under different abiotic stress conditions and plant organs. The combination of EF-1 alpha and GAPDH was recommended as the best reference genes for gene expression studies in M. officinalis.
Article
Biotechnology & Applied Microbiology
Jeongah Song, Jeonghee Cho, Jeongsik Park, Jeong Ho Hwang
Summary: This study identified stable reference genes in seven tissues across four developmental stages in minipigs, which can be used to evaluate mRNA expression levels. HPRT1 and 18S were found to be the most stable genes. The expression levels of ACE2 in the intestine and kidney increased significantly at PND28, consistent with the ACE2 expression pattern in humans.
Article
Biochemistry & Molecular Biology
Yuanmiao Wei, Yao Liu, Ling Li, Shuna Xiang, Hanyue Zhang, Ying Shang
Summary: This study identified s9ap as an ideal endogenous reference gene for the detection of Pleurotus eryngii. The method was also suitable for processed food products.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Chemistry, Applied
Tingting Li, Jishi Wang, Zhiying Wang, Lu Qiao, Rui Liu, Shanshan Li, Ailiang Chen
Summary: Mitochondrial genes are commonly used for PCR-based meat adulteration authentication, but variations in gene copies can lead to quantification errors. This study developed a quantitative method using single-copy nuclear genes to accurately identify mutton adulteration, achieving high recovery rates for simulated mutton-pork, mutton-chicken, and mutton-duck samples.
Article
Food Science & Technology
Ling Li, Yuanmiao Wei, Yao Liu, Shuna Xiang, Hanyue Zhang, Ying Shang
Summary: In this study, the matB gene was identified as a species-specific gene of L. edodes, serving as an ideal reference gene for qualitative and quantitative PCR assays to detect L. edodes material. This provides a convenient and accurate method for detecting L. edodes products and adulteration in wild edible mushroom products.
FOOD SCIENCE & NUTRITION
(2022)
Article
Food Science & Technology
Chen Zhang, Zhan Lei, Yinjiao Li, Lunzhao Yi, Ying Shang
Summary: The Tps2 gene has been identified as a reliable, convenient, and accurate endogenous reference gene for detecting components of Flammulina velutipes, even in processed food products such as sauce and biscuits, using PCR techniques. Quantitative PCR analysis using both SYBR Green and Taqman probe showed high homogeneity of the target Tps2 sequence in different F. velutipes varieties, with a low detection limit of 3 pg/uL of DNA template.
FOOD ANALYTICAL METHODS
(2021)
Article
Multidisciplinary Sciences
Meng Wang, Tingting Ren, Prince Marowa, Haina Du, Zongchang Xu
Summary: This study identified stable reference genes PP2A and TUA5 in S. glauca under different salt concentrations and tissues, providing a reliable method for normalizing gene expression. These reference genes were validated in germinating seeds of S. glauca, highlighting their utility in accurate gene expression profiling.
SCIENTIFIC REPORTS
(2021)
Article
Agronomy
Junliang Yin, Lu Hou, Xinchen Jiang, Jie Yang, Yang He, Xiaokang Zhou, Xiongmeng Zhu, Andong Gong, Yongxing Zhu, Zhongyi Chen
Summary: Alligatorweed is an invasive semiaquatic weed that poses a serious threat to agricultural production and ecological balance. This study identified stable reference genes for gene expression analysis in A. philoxeroides, laying a foundation for further research on gene expression profiling and invasive weed management.
Article
Horticulture
Chenjie Zhang, Chengwei Song, Linfeng Chen, Huili Ma, Yabing Zhang, Dalong Guo, Lili Guo, Xiaogai Hou
Summary: The aim of this study was to identify suitable reference genes for normalizing the expression levels of miRNA in different developmental stages and tissues. Five algorithms were used for stability analysis, and the results identified mtr-MIR160b-p3 and gma-miR394a-5p as the most stable miRNAs during bud development in early-flowering tree peony 'Feng dan', while PC-5p-19095 was the most stable during bud development in late-flowering tree peony 'Lian he'. Additionally, mtr-miR159a was found to be the most stable miRNA during flower development stages of different tree peony varieties.
Article
Food Science & Technology
Yao Liu, Ling Li, Yuanmiao Wei, Hanyue Zhang, Shuna Xiang, Ying Shang
Summary: The article introduces a detection method for T. albuminosus mushrooms using the TSA gene as an endogenous reference gene. The method is validated through qualitative and quantitative PCR, providing a fast and accurate way to detect T. albuminosus and protect consumers' rights while promoting the industry.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Xiujie Yin, Taotao He, Kun Yi, Yihang Zhao, Yao Hu, Jiaxue Liu, Xiaomeng Zhang, Lingdong Meng, Lina Wang, Haoyue Liu, Yonggang Li, Guowen Cui
Summary: Twelve reference genes were selected based on transcriptomic data for stable expression analysis in various organs of Caucasian clover under different stress conditions. Results showed that different reference genes were most stable under different stress conditions, with RCD1 and NLI2 identified as the most stable reference genes across all organs.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Xiaolin Jiang, Yong Jiang, Feng Ye
Summary: This study used real-time quantitative PCR to detect Mucorales and Aspergillus in formalin-fixed, paraffin-embedded samples. The combination of Mucorales RQ-PCR and Aspergillus RQ-PCR was recommended as a screening tool for detecting suspected mucormycosis and/or aspergillosis samples.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Ecology
Agnes Vallier, Elisa Dell'Aglio, Mariana Galvao Ferrarini, Ophelie Hurtado, Carole Vincent-Monegat, Abdelaziz Heddi, Rita Rebollo, Anna Zaidman-Remy
Summary: This study used transcriptomic high-throughput analysis to identify a subset of stably expressed genes from different developmental stages of the gut and associated-bacteriomes of Sitophilus oryzae, which can be used as reference genes in qRT-PCR experiments. Two genes, TAO and YTH3, were selected as suitable genes for comparing transcript abundance in symbiotic and aposymbiotic insects at various developmental stages. These well-conserved genes have the potential to be applied in other insect species, emphasizing the importance of using unbiased methods for reference gene selection.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2023)
Article
Agriculture, Dairy & Animal Science
Kanakachari Mogilicherla, Rajendra P. Athe, Rudra N. Chatterjee, Tarun K. Bhattacharya
Summary: The study identified and validated the most stable reference genes in different organ tissues of chicken, which play a crucial role in data normalization and accurate quantification using qPCR. Selection of individual or combination of reference genes should be tailored based on the specific tissue for effective data normalization.
Article
Biochemistry & Molecular Biology
Kathannan Sankar, Hyung Joo Yoon, Young Bo Lee, Kyeong Yong Lee
Summary: Bumble bees are important alternative pollinators and model insects. This study identified stable reference genes for the bumble bee species and their different lines and tissues using RT-qPCR procedures and four analysis programs. The results emphasized the need to evaluate the stability of candidate reference genes for any differently designed lines and tissue conditions in bumble bee species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Brian M. Moloney, Peter F. McAnena, Sami M. Abd Elwahab, Angie Fasoula, Luc Duchesne, Julio D. Gil Cano, Catherine Glynn, AnnaMarie O'Connell, Rachel Ennis, Aoife J. Lowery, Michael J. Kerin
Summary: This study evaluated the clinical application of a low-power electromagnetic wave microwave breast imaging (MBI) system. The results showed that the MBI system was able to detect and localize the majority of breast lesions, including benign lesions and breast cancer. Patients reported a positive experience with the system, and no device-related adverse events were recorded. This study suggests that the MBI system has the potential to serve as a noninvasive, non-ionizing, and painless adjunct to breast cancer diagnosis.
ACADEMIC RADIOLOGY
(2022)
Article
Medicine, General & Internal
Evan J. Fahy, Conor M. Sugrue, Deirdre Jones, Padraic Regan, Alan Hussey, Shirley Potter, Michael Kerin, Niall M. McInerney, Jack Kelly
Summary: Cutaneous squamous cell carcinoma (SCC) of the scalp is a potentially fatal disease that is more common in elderly males from rural communities. Patients living further from care tend to present with larger tumors.
IRISH JOURNAL OF MEDICAL SCIENCE
(2022)
Review
Oncology
Domhnall J. O'Connor, Matthew G. Davey, Laura R. Barkley, Michael J. Kerin
Summary: Patients with invasive lobular carcinoma of the breast are less likely to achieve complete pathological response, receive breast-conserving surgery, and have negative margin status compared to patients with invasive ductal carcinoma.
Article
Oncology
Matthew G. Davey, Eoin Kerin, C. O'Flaherty, Elizabeth Maher, Vinitha Richard, Peter McAnena, Ray P. McLaughlin, Karl J. Sweeney, Michael K. Barry, Carmel M. Malone, William Wyns, Osama Soliman, Nicola Miller, Maccon M. Keane, Aoife J. Lowery, Michael J. Kerin
Summary: Pathological complete response (pCR) is a sensitive biomarker and surrogate to survival outcomes in HER2+ breast cancer, with predictors including tumor size, progesterone receptor status, ductal carcinoma, and grade 3 disease. Breast and axillary pCR predict enhanced disease-free and overall survival, with axillary pCR independently predicting improved overall survival.
Article
Clinical Neurology
Sean Curran, David O'Brien, Michael Kerin
Summary: This study examined the exposure of undergraduate medical students in Irish medical schools to neurosurgery and their desire for greater emphasis on this specialty. The findings suggest that organizing neurosurgical lecture days increased students' interest in further learning and clinical exposure to neurosurgery.
BRITISH JOURNAL OF NEUROSURGERY
(2022)
Article
Medicine, General & Internal
Matthew G. Davey, John P. M. O'Donnell, Elizabeth Maher, Cliona McMenamin, Peter F. McAnena, Michael J. Kerin, Nicola Miller, Aoife J. Lowery
Summary: The study identified significant differences in the perception of GDPR and willingness to consent to data being used in clinical research between doctors and patients. Doctors are more willing to allow their personal data to be processed anonymously and require less explicit consent compared to patients. Patient's level of education has a significant impact on GDPR awareness.
IRISH JOURNAL OF MEDICAL SCIENCE
(2022)
Article
Oncology
Matthew G. Davey, Gerard Feeney, Heidi Annuk, Maxwell Paganga, Emma Holian, Aoife J. Lowery, Michael J. Kerin, Nicola Miller
Summary: This study aimed to evaluate the role of mi(cro)RNAs as biomarkers in predicting nodal status and recurrence in colorectal cancer patients. The results showed that aberrant expression of miR-21 and miR-135b was associated with increased nodal stage, while increased expression of miR-195 predicted the time to disease recurrence. These findings support the use of miRNA expression profiling in personalizing therapeutic decision making.
Review
Surgery
Matthew G. Davey, Ferdia Browne, Nicola Miller, Aoife J. Lowery, Michael J. Kerin
Summary: This study confirms that achieving a pathological complete response (pCR) is associated with improved survival outcomes in patients with HER2(+) breast cancer. Patients who achieved pCR had significantly better event-free survival, recurrence-free survival, and overall survival compared to those with residual disease. These findings support the use of pCR as a biomarker for survival and as an appropriate endpoint for clinical research.
Review
Surgery
Matthew G. Davey, Colm O'Flaherty, Eoin F. Cleere, Aoife Nohilly, James Phelan, Evan Ronane, Aoife J. Lowery, Michael J. Kerin
Summary: Axillary lymph node status is a strong prognostic indicator in invasive breast cancer, but the necessity of sentinel lymph node biopsy (SLNB) for ductal carcinoma in situ (DCIS) is unclear. This study aimed to determine the likelihood of SLNB positivity in DCIS patients and identify predictive parameters.
Article
Oncology
Leonor Rodriguez, Pat Dolan, Michael Kerin, AnnMarie Groarke
Summary: This study explores the unmet needs of mothers and their adolescent children facing cancer. The research finds that cancer presents an emotional burden for both mothers and children, with difficulties in dealing with emotions related to cancer recurrence. Mothers struggle to identify their children's unmet needs and lack the skills to approach them, adding to their illness burden and feelings of guilt.
EUROPEAN JOURNAL OF ONCOLOGY NURSING
(2023)
Review
Biochemistry & Molecular Biology
Luis Bouz Mkabaah, Matthew G. Davey, James C. Lennon, Ghada Bouz, Nicola Miller, Michael J. Kerin
Summary: Identifying patients at risk of breast cancer recurrence is difficult, making the discovery of biomarkers for diagnosis crucial. MiRNAs, small non-coding RNA molecules, have shown promise as biomarkers in cancer. A systematic review identified 44 miRNAs that can predict breast cancer recurrence. Increased expression of 25 miRNAs and decreased expression of 14 miRNAs were observed in patients with recurrence. In addition, there were five miRNAs with inconsistent expression levels. MiRNA expression patterns can accurately predict breast cancer recurrence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Raza Abbas Syed, Matthew G. Davey, Vinitha Richard, Nicola Miller, Michael J. Kerin
Summary: Contemporary breast cancer management includes personalized treatment approaches and focuses on miRNAs as potential biomarkers for predicting treatment-related cardiovascular toxicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Surgery
Matthew G. Davey, Ferdia Browne, Martin S. Davey, Stewart R. Walsh, Michael J. Kerin, Aoife J. Lowery
Summary: This study conducted a systematic review and network meta-analysis to evaluate the optimal neck closure method after thyroid and parathyroid surgery. The results showed that subcuticular sutures provide better patient-reported outcomes and cosmesis compared to other closure methods, without compromising the safety of the procedure.
Review
Surgery
Eoin F. Cleere, Matthew G. Davey, Thomas J. Crotty, Orla Young, Aoife J. Lowery, Michael J. Kerin
Summary: Patients with a history of bariatric surgery have a significantly increased risk of hypocalcaemia after thyroidectomy, and the management of hypocalcaemia in these patients is challenging.
BRITISH JOURNAL OF SURGERY
(2022)
Article
Cell & Tissue Engineering
Ritihaas Surya Challapalli, Roisin M. Dwyer, Niall McInerney, Michael J. Kerin, Aoife J. Lowery
Summary: Tissue engineering using Adipose Derived Stromal Cells (ADSCs) is a promising approach for soft tissue regeneration, particularly in post-breast cancer reconstruction. However, concerns remain regarding the oncological safety of using ADSCs in cancer patients.
STEM CELL REVIEWS AND REPORTS
(2021)