Article
Fisheries
Shun Zhou, Qing Huang, Mingming Yu, Ying Li, Xujia Zheng, Yunji Xiu
Summary: The study developed a rapid detection method, LAMP-HNB assay, for Aeromonas salmonicida, an important pathogen in fish farming, with good specificity and high sensitivity. The method has potential applications in diagnostic testing, health certification, and surveillance programs.
JOURNAL OF FISH DISEASES
(2021)
Article
Biochemical Research Methods
Xuliang Zhang, Yingfeng Zhao, Chang Ma, Wei Wu, Min Dong, Jinwei You, Jie Liu, Shifeng Yun
Summary: In this study, a rapid and visual detection method for minute virus of mice (MVM) using loop-mediated isothermal amplification (LAMP) with hydroxynaphthol blue (HNB) dye was developed. The assay showed high sensitivity and specificity, with a lower limit of detection compared to conventional PCR. The established LAMP system was successfully applied to detect MVM infections in laboratory animal samples.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Infectious Diseases
Sayamon Hongjaisee, Natteewan Doungjinda, Woottichai Khamduang, Tanawan Samleerat Carraway, Jiraprapa Wipasa, Jose D. Debes, Khuanchai Supparatpinyo
Summary: The study developed a reverse transcription loop-mediated isothermal amplification (RTLAMP) assay for detecting hepatitis C virus (HCV) in a single closed tube. The assay showed high sensitivity, specificity, and stability in detecting different HCV genotypes, making it a potentially useful tool for identifying individuals in need of HCV treatment, particularly in resource-limited settings.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Multidisciplinary Sciences
Ilada Choopara, Yothin Teethaisong, Narong Arunrut, Sudaluck Thunyaharn, Wansika Kiatpathomchai, Naraporn Somboonna
Summary: The study developed a simple ITS1 LAMP-HNB method for detection of fungi, demonstrating high sensitivity and applicability for preventing opportunistic fungal infections in resource-constrained areas.
Article
Entomology
Yue Zhang, Weisong Li, Massimiliano Virgilio, Marc De Meyer, Zhihong Li
Summary: This study achieved rapid molecular identification of Ceratitis fruit flies using LAMP technology and mitochondrial genes as targets. A loop-mediated isothermal amplification identification technology system was established, which utilized rapid, rough DNA extraction and visual detection. The system demonstrated high sensitivity and simplicity, allowing the entire identification process to be completed within one hour.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Biophysics
Jayeon Song, Hyo Yong Kim, Soohyun Kim, Yujin Jung, Hyun Gyu Park
Summary: A novel technology, SP-HAMP, is described for target nucleic acid detection under isothermal conditions. By designing a unique hairpin probe, it is possible to detect target DNA with outstanding discrimination capability using only a single primer, without the need for complex multiple primers.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Huan-Huan Zhu, Yuan Li, Li-Xia Wu, Ke-Sheng Wang, Yue Zhang, Qiang-Yuan Fan, Zun-Zhen Ming, Wei-Qin Chen, Wei-Wei Liu
Summary: Loop-mediated isothermal amplification (LAMP) is a promising diagnostic tool for genetic amplification, known for its speed, simplicity, efficiency, and sensitivity. This research introduces an internal heating method for LAMP using magnetic nanoparticles (MNPs) called nano-LAMP, which can detect low concentrations of HPV and has been validated in clinical samples.
Review
Chemistry, Analytical
Jesse M. M. Marangoni, Kenneth K. S. Ng, Arezoo Emadi
Summary: Loop-mediated isothermal amplification (LAMP) is a useful tool for pathogen detection in clinical and environmental samples, but monitoring LAMP can be challenging for point-of-use applications. This review discusses the use of voltammetric sensors for LAMP monitoring and highlights various strategies for detecting DNA amplicons, pyrophosphate, and protons. The review also addresses the hurdles in adapting these sensors for on-site applications.
Article
Chemistry, Analytical
Meng Chen, Qidi He, Yanli Tong, Zuanguang Chen
Summary: This study successfully combined LAMP with RCA to achieve rapid detection and quantification of pathogens. By using a strand displacement strategy in a single-tube reaction, different pathogen target genes were successfully detected, demonstrating the universality and reliability of the method.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Plant Sciences
R. Logeshwari, C. Gopalakrishnan, A. Kamalakannan, J. Ramalingam, R. Saraswathi
Summary: A rapid and highly specific detection method based on LAMP technology was developed in this study for the seed-borne pathogen Sarocladium oryzae. This method offers quick and reliable detection of the pathogen, which is important for maintaining the health of rice seeds.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Sandra V. Gomez-Gutierrez, Stephen B. Goodwin
Summary: LAMP is a molecular technique for detection of plant pathogens, known for its specificity, sensitivity, and ability to conduct reactions at a constant temperature. This review explains the amplification process, visualization techniques, advantages, and disadvantages of LAMP compared to alternative isothermal approaches. It also presents the application of LAMP in detecting fungal pathogens and viruses in wheat, as well as the development of real-time and multiplex LAMP assays.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Tadafumi Yokoyama, Yuko Tasaki, Natsumi Inoue, Naotoshi Sugimoto, Eri Nariai, Sanae Kuramoto, Taizo Wada
Summary: This study developed a RT-LAMP technique for the diagnosis of HPeVs, particularly HPeV3, allowing for rapid and convenient detection of HPeV3 RNA. The technique showed clinical utility in diagnosing newborns and infants with sepsis-like symptoms.
Review
Chemistry, Analytical
So-Hee Kim, So-Young Lee, Unji Kim, Se-Wook Oh
Summary: This review outlines several techniques for reducing false-positive LAMP results before and after amplification. Organic additives or pullulan can be used to decrease nonspecific amplification before amplification. Uracil-DNA-glycosylase and the hot-start effect with gold nanoparticles can eliminate carry-over contamination and reduce nonspecific amplification after amplification. The use of guide RNA and colorimetric change in DNAzyme can accurately confirm amplification results.
ANALYTICA CHIMICA ACTA
(2023)
Review
Chemistry, Analytical
Poramin Boonbanjong, Kiatnida Treerattrakoon, Wassa Waiwinya, Piyawat Pitikultham, Deanpen Japrung
Summary: Isothermal amplification (IA) is a nucleic acid amplification technology that has significantly contributed to the healthcare system by improving disease diagnosis through higher sensitivity, specificity, and rapidity. Certain IA techniques provide a rapid, sensitive, specific, and simple method for disease diagnosis. However, not all IA techniques have been widely used in clinical applications due to the influence of different biomarkers and sample types on the IA system.
Article
Plant Sciences
Xiayan Pan, Xiao Wang, Junjie Yu, Mina Yu, Huijuan Cao, Mingli Yong, Tianqiao Song, Zhongqiang Qi, Yan Du, Rongsheng Zhang, Yongfeng Liu
Summary: In this study, a rapid LAMP assay was developed to detect the mating type of Villosiclava virens, the causal agent of rice false smut. The LAMP assay showed high sensitivity and specificity, with the ability to detect small amounts of DNA and provide results in just 45 minutes. It can serve as a rapid testing tool for mating type recognition of V. virens isolates in the field.
Article
Biotechnology & Applied Microbiology
Yajing Liang, Beibei Li, Qi Zhang, Shilong Zhang, Xiaoqing He, Libo Jiang, Yi Jin
Summary: Through ecological experiments and genome wide association studies, we found that different genotypes of Escherichia coli and Staphylococcus aureus exhibit different growth trajectories in co-culture, with significant SNPs potentially playing key roles in bacterial interactions.
Article
Multidisciplinary Sciences
Dengcheng Yang, Yi Jin, Xiaoqing He, Ang Dong, Jing Wang, Rongling Wu
Summary: The study develops a framework to understand the impact of SNPs on bacterial response and their interactions. By investigating the genetic influence on phenotypic plasticity, the study reveals the independent and dependent effects of SNPs on phenotypes.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Qiangsheng Lu, Congcong Le, Xiaoqian Zhang, Jacob Cook, Xiaoqing He, Mohammad Zarenia, Mitchel Vaninger, Paul F. Miceli, David J. Singh, Chang Liu, Hailang Qin, Tai-Chang Chiang, Ching-Kai Chiu, Giovanni Vignale, Guang Bian
Summary: This study proposes a new method to achieve flat band physics in monolayer graphene by substrate modulation. Experimental results on the graphene/SiC heterostructure demonstrate that substrate modulation leads to Dirac fermion cloning and the proximity of the two Dirac cones in monolayer graphene. Theoretical modeling confirms the cloning mechanism and predicts the emergence of moire flat bands at certain magic lattice constants of the substrate. This study suggests that epitaxial single monolayer graphene on suitable substrates is a promising platform for exploring exotic many-body quantum phases arising from interactions between Dirac electrons.
ADVANCED MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Jun Bai, Yanxi Liu, Mengjiao Liu, Shiting Luo, Yuhong Cheng, Gang Li, Cheng Liu, Shixiong Wen, Mian Xia, Xiaoqing He, Yi Jin
Summary: This study investigated the potential application of a phage cocktail in controlling Pseudomonas syringae pv. actinidiae (Psa) infection, and the results showed better biological treatment efficacy of the phage cocktail compared to individual phages.
BIOLOGICAL CONTROL
(2022)
Article
Immunology
Xiaoqing He, Yifu Liu, Haoyu Wang, Wei Sun, Yihan Lu, Zhongyan Shan, Weiping Teng, Jing Li
Summary: The highly expressed ENO1-P6 autoantibodies may be important risk factors for miscarriage in euthyroid females with thyroid autoimmunity. The levels of ENO1-P6 autoantibodies in the serum may serve as good predictive markers for pregnancy loss in euthyroid females with thyroid autoimmunity, especially the expression of its IgG2 subclass.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Ganggang Zhao, Yun Ling, Yajuan Su, Zanyu Chen, Cherian J. Mathai, Ogheneobarome Emeje, Alexander Brown, Dinesh Reddy Alla, Jie Huang, Chansong Kim, Qian Chen, Xiaoqing He, David Stalla, Yadong Xu, Zehua Chen, Pai-Yen Chen, Shubhra Gangopadhyay, Jingwei Xie, Zheng Yan
Summary: Laser scribing of metallic conductive transition metal oxides on soft elastomers has been achieved, with high electrical conductivity, biocompatibility, chemical stability, and compatibility with magnetic resonance imaging. This method shows potential for constructing bioelectronics and soft actuators.
Article
Materials Science, Multidisciplinary
Ahmed Al Mayyahi, Swagotom Sarker, Brian M. Everhart, Xiaoqing He, Placidus B. Amama
Summary: In this study, delaminated and OH-terminated MXene was synthesized through a one-step hydrothermal process. The co-catalytic effect of this MXene combined with TiO2 was found to be highly effective in photocatalytic oxidation of NOx. The OH-terminated MXene outperformed the F-terminated MXene in terms of NO2 storage selectivity and DeNOx index.
MATERIALS TODAY COMMUNICATIONS
(2022)
Article
Immunology
Wei Sun, Yan Feng, Hui Li, Xiaoqing He, Yihan Lu, Zhongyan Shan, Weiping Teng, Jing Li
Summary: This study found that high levels of ENO1Ab in the maternal circulation can affect the brain development of offspring, leading to impaired learning and memory abilities.
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2022)
Article
Environmental Sciences
Yan Yang, Kexin Cheng, Kaihang Li, Yi Jin, Xiaoqing He
Summary: This study comprehensively investigated the diversity patterns and assembly processes of rare and abundant taxa in a wetland ecosystem, revealing the significant factors affecting community structure and the prevalent metabolic pathways in the wetland samples. The study also found that nonrare taxa exhibited more interactions with rare taxa, and the assembly mechanisms differed between rare and abundant taxa. These findings contribute to our understanding of microbial dynamics and have important implications for wetland management.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Nanoscience & Nanotechnology
Nikhila C. Paranamana, Ryan Gettler, Henry Koenig, Stephen Montgomery-Smith, Xiaoqing He, Matthias J. Young
Summary: Understanding the atomic structure of ultrathin ALD coatings is crucial for establishing structure-property relationships and stabilizing battery interfaces. This study used cryogenic scanning transmission electron microscopy to directly measure atomic structural variations across the depth of an ALD AlOx film deposited onto a carbon nanotube substrate. The results confirmed a proposed two-phase bulk/interface structural model and revealed a thicker interfacial layer than previously reported.
ACS APPLIED NANO MATERIALS
(2022)
Article
Behavioral Sciences
Lin Zhao, Dongdong Zhou, Lingli Ma, Jinhui Hu, Ran Chen, Xiaoqing He, Xinyu Peng, Zhenghao Jiang, Liuyi Ran, Jiaojiao Xiang, Qi Zeng, Yi Zhou, Linxi Dai, Qi Zhang, Su Hong, Wo Wang, Li Kuang
Summary: Nonsuicidal self-injury (NSSI) is a serious risk behavior in adolescents and is strongly associated with suicide. This study examined the behavioral and neural responses in adolescents with NSSI when exposed to negative emotional stimuli using a two-choice oddball paradigm. The results showed that adolescents with NSSI exhibited distinct neural responses, specifically in the N250, P300, and LPP components, during negative emotional face stimulation. Additionally, the study found evidence of brain lateralization effects in both the N250 and LPP components.
BEHAVIOURAL BRAIN RESEARCH
(2023)
Article
Geochemistry & Geophysics
Xiaoqing He, Ziyao Fang, Yunpei Gao, Xi Yu, Yanan Shen, Liping Qin
Summary: The redox states of the ocean and atmosphere during the Ediacaran Period remain controversial. This study presents systematic Cr isotopic data for two Ediacaran successions in South China, revealing that the ocean redox conditions were highly stratified and heterogeneous. The positively fractionated 853Cr values indicate higher atmospheric oxygen and enhanced biological productivity during this period.
GEOCHIMICA ET COSMOCHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Jiahui Guo, Yihan Lu, Xiaoqing He, Jiashu Li, Chenling Fan, Hongmei Zhang, Weiping Teng, Zhongyan Shan, Jing Li
Summary: Our recent study found that ENO1Ab expression in an experimental AIT mouse model was induced by thyroglobulin (Tg) immunization. In this study, we investigated the potential roles of ENO1Ab in miscarriage occurrence among AIT women and the specific epitopes of ENO1 targeted by ENO1Ab. The results showed that serum ENO1Ab was an independent risk factor for miscarriage, especially among AIT females, and the autoantibody specific to the P6 epitope of ENO1 may be more specifically related to this disorder.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Psychiatry
Lin Zhao, Dongdong Zhou, Jinhui Hu, Xiaoqing He, Xinyu Peng, Lingli Ma, Xinyi Liu, Wanqing Tao, Ran Chen, Zhenghao Jiang, Chenyu Zhang, Jing Liao, Jiaojiao Xiang, Qi Zeng, Linxi Dai, Qi Zhang, Su Hong, Wo Wang, Li Kuang
Summary: In this study, changes in microstate parameters in depressed adolescents with nonsuicidal self-injury (NSSI), depressed adolescents, and healthy adolescents during exposure to negative emotional stimuli were compared. The effects of repetitive transcranial magnetic stimulation (rTMS) on clinical symptoms and microstate parameters were also explored in depressed adolescents with NSSI. The findings provide evidence for potential mechanisms and treatment optimization for NSSI behaviors in adolescents.
FRONTIERS IN PSYCHIATRY
(2023)
Article
Biotechnology & Applied Microbiology
Chenwu Xiong, Hua Tong, Xiaoqing He, Dewu Ding
Summary: In this study, we integrated the transcriptome changes of Shewanella oneidensis MR-1 under different oxygen levels into its protein-protein interaction network to investigate the specific changes under these conditions. The results showed that most of the key nodes in the network were ribosomes or proteins closely related to ribosomes under high and low oxygen levels. Additionally, we analyzed the changes in protein-protein interactions and identified key interactions in three pairs of active networks. This study provides guidance for identifying key proteins and interactions under different conditions for this species.
ANNALS OF MICROBIOLOGY
(2023)
