Article
Food Science & Technology
Hoang Minh Duc, Yu Zhang, Hoang Minh Son, Hung-Hsin Huang, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto
Summary: In this study, a lytic phage STG2 capable of reducing S. enteritidis and S. typhimurium contaminations on cabbage and various surfaces was characterized. Genomic characterization revealed that phage STG2 does not contain any genes associated to antibiotic resistance, toxins, lysogeny, or virulence factors. Additionally, phage STG2 exhibited great efficacy in reducing both planktonic cells and biofilms on different surfaces.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Review
Food Science & Technology
Haojie Ge, Shasha Fu, Huimin Guo, Maozhi Hu, Zhengzhong Xu, Xiaohui Zhou, Xiang Chen, Xin'an Jiao
Summary: In recent years, there has been an increase in foodborne diseases caused by pathogens. Bacteriophages (phages) have the potential to play a significant role in preventing, controlling, and treating these diseases due to their advantages such as inhibiting pathogenic bacteria and prolonging the shelf life of food. However, there are still challenges in the practical application of phages in the food industry.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Review
Food Science & Technology
Justin Falardeau, Aljosa Trmcic, Siyun Wang
Summary: Listeria monocytogenes poses a food safety risk in ready-to-eat foods like fresh and soft/semisoft cheeses. Researchers have investigated various biocontrol strategies such as bacteriocins, bacteriophages, and competition with native microbiota to inhibit the growth of L. monocytogenes in susceptible cheeses. While these strategies have shown success in reducing L. monocytogenes populations in cheeses, regrowth of surviving cells remains a common issue.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2021)
Article
Veterinary Sciences
Napakhwan Imklin, Pattaraporn Sriprasong, Sahathat Phuttapatimok, Tanyanant Kaminsonsakul, Yonlayong Woonwong, Pichai Jirawattanapong, Porntippa Lekcharoensuk, Narut Thanantong, Rujikan Nasanit
Summary: In this study, 6 phages were isolated and tested for their efficacy against E. coli strains isolated from diarrheal pigs. Phage therapy was found to significantly reduce fecal E. coli counts and restore the gut's bacterial composition. Additionally, giving phage cocktails resulted in higher average daily gain in piglets.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Infectious Diseases
Tengteng Li, Hong Chen, Jiayi Zhao, Zhenxiang Tao, Weiqing Lan, Yong Zhao, Xiaohong Sun
Summary: In this study, a novel Salmonella phage vB_SalM_SPJ41 was isolated from poultry farms in Shanghai, China. It was found that this phage could lyse multiple serotypes of antibiotic-resistant S. enterica and effectively inhibit the biofilm produced by antibiotic-resistant S. enterica. Additionally, the phage showed promising results in reducing the levels of S. Enteritidis and S. Derby in lettuce and salmon at low temperatures. Genomic analysis revealed the absence of virulence factor genes or antibiotic resistance genes in the phage.
Article
Microbiology
Ahmed Esmael, Ehab Azab, Adil A. Gobouri, Mohamed A. Nasr-Eldin, Mahmoud M. A. Moustafa, Shereen A. Mohamed, Omnia A. M. Badr, Alzahraa M. Abdelatty
Summary: Foodborne salmonellosis is a global threat, and in this study, two broad-spectrum lytic Salmonella phages, SPHG1 and SPHG3, were isolated and characterized for their ability to infect multidrug-resistant Salmonella Typhimurium. These phages showed strong lytic activity with a short lysis time and high burst sizes, and were stable under various environmental conditions. A phage cocktail containing SPHG1 and SPHG3 significantly reduced the viable count of S. Typhimurium in milk, water, and chicken breast, demonstrating their potential as biocontrol agents against foodborne salmonellosis.
Article
Food Science & Technology
Fei Ma, Yu Ning, Qingqing Wan, Likou Zou, Yuntao Liu, Shujuan Chen, Jianlong Li, Zhen Zeng, Yong Yang, Hong Chen, Min Zhou, Shuliang Liu, Cheng Li, Aiping Liu
Summary: Bacteriophages LSA2308 and LSA2366 showed lytic activity against drug-resistant S. aureus, and combined with ampicillin achieved better clearance in LB broth. In skim milk, a concentration of 10^7 PFU/mL bacteriophages exhibited the most effective bactericidal efficacy against S. aureus strains.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Microbiology
Kishani N. Naligama, Anupama P. Halmillawewa
Summary: Pectobacterium carotovorum phage vB_PcaM_P7_Pc (P7_Pc) isolated from infected carrot samples in Sri Lanka was comprehensively characterized and classified under the genus Certrevirus. This study provides a foundation and theoretical basis for the application of P7_Pc as a biocontrol agent.
MICROBIOLOGY SPECTRUM
(2022)
Article
Plant Sciences
Manoj Choudhary, Jorge Pereira, Edwin B. Davidson, James Colee, Swadeshmukul Santra, Jeffrey B. Jones, Mathews L. Paret
Summary: Bacteriophages are effective biocontrol agents for managing bacterial diseases in plants, but their persistence on plant surfaces is limited due to degradation by UV exposure. This study shows that coating phages with N-acetylcysteine surface-coated manganese-doped zinc sulfide (NAC-ZnS) nanomaterial can protect them from UV degradation, enhance their lytic activity against bacteria, and improve their persistence on plant surfaces.
Article
Virology
Maroua Oueslati, Dominique Holtappels, Kiandro Fortuna, Mohamed Rabeh Hajlaoui, Rob Lavigne, Najla Sadfi-Zouaoui, Jeroen Wagemans
Summary: In this study, a novel lytic bacteriophage SoKa was isolated, which can effectively lyse Pss and related strains, and possesses characteristics of resistance to low temperature, UV radiation, and biofilm. SoKa has the potential for application in disease control in citrus production.
Article
Microbiology
D. Holtappels, K. J. Fortuna, M. Vallino, R. Lavigne, J. Wagemans
Summary: The increasing interest in using bacteriophages for therapeutic purposes has led to a significant rise in the number of sequenced phage genomes. The International Committee on Taxonomy of Viruses has proposed a comprehensive and data-driven classification system for phages, which facilitates their taxonomy. In this study, we present the taxonomical classification of a novel Xanthomonas phage FoX4, isolated from a Brussels sprouts field in Belgium. The phage has a limited ability to lyse bacteria but efficiently adsorbs to its host. Based on its genome sequence and low similarity to known phages, the phage is classified as a new phage genus called Foxquatrovirus.
Article
Multidisciplinary Sciences
Marta Nisita Dewanggana, Clare Evangeline, Maurita Delia Ketty, Diana Elizabeth Waturangi, Yogiara, Stella Magdalena
Summary: Bacteriophage treatment is an effective food preservation method. This study focused on using bacteriophages isolated from Indonesian traditional Ready-to-Eat (RTE) food to control Enterotoxigenic Escherichia coli (ETEC) population in various foods. The molecular characterization and bioinformatic analysis showed that the bacteriophage has the potential to be a promising biocontrol agent. The results also demonstrated that the bacteriophage significantly reduced the ETEC population in different food samples after different incubation times.
SCIENTIFIC REPORTS
(2022)
Review
Plant Sciences
Jeroen Wagemans, Dominique Holtappels, Eeva Vainio, Mojgan Rabiey, Cristina Marzachi, Salvador Herrero, Mohammadhossein Ravanbakhsh, Christoph C. Tebbe, Mylene Ogliastro, Maria A. Ayllon, Massimo Turina
Summary: Viruses can be used as biological control agents to combat biotic stresses in crop production, but their potential is limited due to regulatory hurdles and a limited market.
ANNUAL REVIEW OF PHYTOPATHOLOGY
(2022)
Article
Microbiology
Yuting Shang, Qifan Sun, Hanfang Chen, Qingping Wu, Moutong Chen, Shuanghong Yang, Mingzhu Du, Fei Zha, Qinghua Ye, Jumei Zhang
Summary: The phage vB_SalP_TR2 showed high lytic potential against Salmonella serovar Albany and other tested serovars, it also exhibited tolerance to high temperature and pH. Additionally, treatment with phage vB_SalP_TR2 reduced the number of Salmonella in food matrices, highlighting its potential as an antibacterial agent for controlling Salmonella in food samples.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Food Science & Technology
Chuan-Wei Tung, Zabdiel Alvarado-Martinez, Zajeba Tabashsum, Arpita Aditya, Debabrata Biswas
Summary: Salmonella enterica is the most common foodborne pathogen worldwide, and controlling its multiple serovars is necessary due to antibiotic resistance. In this study, a novel bacteriophage-1252 was isolated and found to have high lytic activity against multiple serovars of S. enterica. It belongs to the Myoviridae family and has stable activity within a certain temperature range. These findings suggest that phage-1252 is a promising candidate for controlling S. enterica serovars in food production.