Review
Microbiology
Amber Grace, Rajnish Sahu, Donald R. R. Owen, Vida A. A. Dennis
Summary: Pseudomonas aeruginosa is a common pathogen that has been identified as a multi-drug resistant strain. PAO1 and PA14, two laboratory strains, have contributed significantly to our understanding of the bacterium. However, there are discrepancies between their behaviors and therapeutic susceptibilities observed in human infections. This review aims to compare the two strains and propose methods to improve their clinical relevance.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Alexandra J. J. Lee, Georgia Doing, Samuel L. L. Neff, Taylor Reiter, Deborah A. A. Hogan, Casey S. S. Greene
Summary: This article studies the genomic heterogeneity of Pseudomonas aeruginosa strains, compares the expression patterns of core genes and accessory genes between PAO1 and PA14 strains, and finds differences in the expression patterns of some core genes among different strains.
Article
Microbiology
Swetha Kasetty, Stefan Katharios-Lanwermeyer, George A. O'Toole, Carey D. Nadell
Summary: Pseudomonas aeruginosa strains PA14 and PAO1 use different strategies for biofilm formation, leading to distinct advantages in competing for surface colonization. PAO1 dominates during early colonization, while PA14 is more effective in colonizing preformed biofilms.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Melanie Grosjean, Sophie Guenard, Caroline Giraud, Cedric Muller, Patrick Plesiat, Paulo Juarez
Summary: Pseudomonas aeruginosa is commonly used to study antibiotic resistance and pathogenesis, but its natural resistance to antibiotics and ability to produce virulence factors hinder its biotechnological potential. By reducing the genome of this bacterium, researchers developed hypovirulent and hypersusceptible recombinant DNA hosts, laying the foundation for further optimization for biotechnological applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Mengmeng Cheng, Ruiyi Chen, Lisheng Liao
Summary: This study investigates the impact of a gene variation, LasR(Q45stop), in Pseudomonas aeruginosa. The introduction of LasR(Q45stop) in the bacteria led to the development of elastase-phenotypic-variability variants during co-evolution with the wild-type. The variation in T2SS-peptidase xcpA and mexT genes played a crucial role in the divergence of various phenotypes. These findings highlight the ongoing microevolution of individual mutational trajectory diversity.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Jieying Pu, Shebin Zhang, Xi He, Jianming Zeng, Cong Shen, Yanfen Luo, Honglin Li, Yifei Long, Jianping Liu, Qian Xiao, Yang Lu, Bin Huang, Cha Chen
Summary: A novel QS regulatory sRNA AmiL has been identified in P. aeruginosa, which influences diverse QS-mediated virulence factors and regulates QS gene expression and C4-HSL production.
MICROBIOLOGY SPECTRUM
(2022)
Article
Virology
Pawel Markwitz, Tomasz Olszak, Grzegorz Gula, Magdalena Kowalska, Michal Arabski, Zuzanna Drulis-Kawa
Summary: This study evaluates the biology and phenotype changes in Pseudomonas aeruginosa PAO1 phage-resistant clones that emerge in phage-treated biofilms, characterizing them for phage-typing patterns, antibiotic resistance, biofilm formation, pathogenicity, and interactions with the innate immune system. Despite the similar phage resistance patterns selected by different phages targeting a particular receptor, there is a dramatic increase in population heterogeneity as a result. The findings also suggest that phage-resistant bacteria are more susceptible to antibiotics and clearance mechanisms by the immune system, supporting the application of phages in therapy.
Article
Infectious Diseases
Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Joanna Kwiecinska-Pirog, Eugenia Gospodarek-Komkowska
Summary: The study investigated the frequency and genotype distribution of 10 virulence factor genes in 107 non-duplicated carbapenem-resistant P. aeruginosa isolates. Variations were observed in the prevalence and distribution of virulence genes, especially in terms of their carbapenemase synthesis ability and the strain origin.
Article
Multidisciplinary Sciences
Sachio Suzuki, Phawinee Subsomwong, Kouji Narita, Noriaki Kawai, Takahito Ishiai, Wei Teng, Rojana Sukchawalit, Akio Nakane, Sadatomo Tasaka, Krisana Asano
Summary: By comparing AbOMVs isolated under hypoxic and normoxic conditions, it was found that they have different protein profiles. While both AbOMVs had similar cytotoxic effects on bacteria, AbOMVs from normoxia induced higher levels of the cytokine TNF-alpha production and invasion of Staphylococcus aureus and Pseudomonas aeruginosa. On the other hand, AbOMVs from hypoxia significantly enhanced A. baumannii invasion into lung epithelial cells. This finding sheds light on the complex interactions between A. baumannii, coinfecting pathogens, and host cells mediated by OMVs, particularly the different pathogenic effects of AbOMVs under normoxic and hypoxic conditions.
Article
Multidisciplinary Sciences
Li Li, Zuxian Xu, Ruipin Cao, Jiaxin Li, Chang-Jer Wu, Yinglu Wang, Hu Zhu
Summary: We investigated the influence of hydroxyl groups on the anti-quorum-sensing (anti-QS) and anti-biofilm activity of cyclic dipeptides against Pseudomonas aeruginosa PAO1. The presence of hydroxyl groups improved the inhibition of virulence factors and cytotoxicity, but reduced the ability to inhibit biofilm formation. These cyclic dipeptides interacted with the QS-related protein LasR, and the introduction of hydroxyl groups enhanced their self-assembly ability.
Article
Immunology
Maria Sultan, Rekha Arya, Akhilesh Kumar Chaurasia, Kyeong Kyu Kim
Summary: This study evaluated the virulence potential of some uncharacterized sensor HK and RR knockouts in P. aeruginosa and demonstrated that KdpD and AauS HK regulate virulence by affecting biofilm formation and motility. Delta kdpD and Delta aauS mutant strains showed reduced biofilm and motility, and increased survival during in vivo infection. The altered expression of the transcriptional regulators and virulence genes elucidated the mechanism by which Delta kdpD and Delta aauS affect virulence in P. aeruginosa.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Carla Perez-Cruz, Ferran Brianso, Elisabeth Sonnleitner, Udo Blaesi, Elena Mercade
Summary: Studies on Pseudomonas aeruginosa PAO1 membrane vesicles (MVs) have shown that the externalization of RNA via MVs mainly occurs during the late exponential phase, suggesting the secretion of different types of MVs during growth processes.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Xiaojuan Tan, Xi Cheng, Jingjing Xiao, Qianqian Liu, Dongsheng Du, Minghui Li, Yang Sun, Jinwei Zhou, Guoping Zhu
Summary: Depletion of inorganic phosphate affects the virulence and biofilm formation of Pseudomonas aeruginosa. The deletion of the lapA gene leads to phenotypic changes and reduces the virulence of PAO1 in both a porcine skin model and Caenorhabditis elegans.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Ronika De, Marvin Whiteley, Rajeev K. Azad
Summary: This article introduces a gene co-expression network-based method for identifying pathogenicity-associated genes. By combining standard protocols with gene network methods, the ability to identify these genes in pathogens can be improved.
Article
Biotechnology & Applied Microbiology
Xuan-He Huang, Meng-Ting She, Yi-Hang Zhang, Yi-Fu Liu, Dong-Xiao Zhong, Yi-Han Zhang, Jun-Xia Zheng, Ning Sun, Wing-Leung Wong, Yu-Jing Lu
Summary: This study found that novel PqsR inhibitors could disrupt the quorum sensing system of Pseudomonas aeruginosa via the pqs pathway, inhibiting the production of virulence factors and enhancing the antimicrobial susceptibility of the bacteria.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Psychology, Clinical
Norin Ahmed, Jessica K. Bone, Gemma Lewis, Nick Freemantle, Catherine J. Harmer, Larisa Duffy, Glyn Lewis
Summary: The study did not find evidence that sertraline alters positive or negative recall in patients with depressive symptoms early in treatment, challenging assumptions of the cognitive neuropsychological model of antidepressant action.
PSYCHOLOGICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Erdem Pulcu, Calum Guinea, Philip J. Cowen, Susannah E. Murphy, Catherine J. Harmer
Summary: This article summarizes the role of the habenula in regulating the expression of anhedonia and discusses the modulatory effect of ketamine on this circuitry. It suggests that experimental paradigms based on reinforcement learning and value-based decision-making can be useful in exploring this network to elucidate the mechanisms underlying ketamine's rapid antidepressant action.
MOLECULAR PSYCHIATRY
(2022)
Article
Neurosciences
Amy L. Gillespie, Chloe Wigg, Indra Van Assche, Susannah E. Murphy, Catherine J. Harmer
Summary: This study found an association between statin use and reduced psychological vulnerability to depression. Long-term use of statins may be a potential prophylactic treatment for depression.
BIOLOGICAL PSYCHIATRY
(2022)
Article
Infectious Diseases
Christopher J. Harmer, Francois Lebreton, Jason Stam, Patrick T. McGann, Ruth M. Hall
Summary: This study examines the causes of antibiotic resistance in the extensively resistant global clone 1 (GC1) Acinetobacter baumannii isolate MRSN 56. The study identified acquired resistance genes at different locations in the chromosome and discovered a new sublineage of GC1 lineage 1 with novel features. The involvement of the mar operon in fluoroquinolone resistance was also noted for the first time.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Genetics & Heredity
Naomi I. Jones, Christopher J. Harmer, Mohammad Hamidian, Ruth M. Hall
Summary: The plasmids from global clone isolates in Greece and Italy were found to have a common ancestor through gene recombination. Factors such as IS26-mediated events, recombination between pdif sites, and homologous recombination have contributed to the evolution and divergence of these plasmids. Gene duplications and losses have been detected, and experimental evidence supports these events.
Article
Multidisciplinary Sciences
Christopher Hodgkins, Laura K. Buckton, Gregory J. Walker, Ben Crossett, Stuart J. Cordwell, Andrea R. Horvath, William D. Rawlinson
Summary: Diagnosis of SARS-CoV-2 infection has primarily relied on RT-PCR and serology, but LC-MS/MS has the potential to provide supplemental data about the presence of viral proteins and improve the diagnosis of viral infection. Researchers developed a proteomics workflow using serum-free culture conditions and successfully detected target virus peptides in clinical samples using LC-MS/MS. This method is an important step towards the use of LC-MS/MS in viral infection diagnosis.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Andreea Raslescu, Sophie Kreicker, Amy L. Gillespie, William Berners-Lee, Susannah E. Murphy, Catherine J. Harmer
Summary: This study provides a dataset of subjective ratings for 150 positive and 150 negative adjectives describing personality characteristics, in order to facilitate word selection and matching in the development of cognitive-emotional tasks. Representative samples of 200 UK-based, English-speaking adults rated the 300 words on self-referential valence, imageability, and subjective frequency through online surveys. Data cleaning and statistical analysis were performed in R. The findings may be particularly relevant for researchers using self-referential cognitive tasks with UK-based samples.
Article
Infectious Diseases
Stephanie J. Ambrose, Pierre Ibri, Christopher J. Harmer, Carol H. Pong, Ruth M. Hall
Summary: The study aimed to characterize an early clinical multiply antibiotic resistant Acinetobacter baumannii global clone 1 (GC1) isolate from Africa. The draft genome sequence of LUH6050 isolate was determined and compared to other early GC1 isolates, revealing the presence of multiple antibiotic resistance genes and plasmids. These data contribute to the understanding of the emergence, evolution, and dissemination of the A. baumannii GC1 clonal complex.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Genetics & Heredity
Christopher J. Harmer, Carol H. Pong, Ruth M. Hall
Summary: Several insertion sequences (IS) showing target specificity were found in various Acinetobacter species. These IS are located near dif modules in plasmids and chromosomal dif sites, and encode a transposase that generates target site duplications (TSDs). The IS identified in the IS1202 group form a distinct family, and other IS in this group have different TSD lengths and may also target dif-like sites.
MICROBIAL GENOMICS
(2023)
Article
Microbiology
Ting L. Luo, Christopher J. Harmer, Francois Lebreton, Jason Stam, Jason W. Bennett, Ruth M. Hall, Patrick T. Mc Gann
Summary: A. baumannii is a significant nosocomial pathogen, especially when resistant to carbapenems. Outbreaks linked to this pathogen are reported globally, especially during times of societal upheaval. This study identified an outbreak of extensively antibiotic-resistant A. baumannii strain in three military treatment facilities.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemical Research Methods
Ashleigh L. Dale, Lok Man, Stuart J. Cordwell
Summary: Lysine acetylation is a reversible post-translational modification that plays a dynamic role in bile resistance and bacterial protein function. The study identified a large number of acetylated lysines in the gastrointestinal pathogen Campylobacter jejuni, with potential implications for its interaction with fibronectin.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Microbiology
Christopher J. J. Harmer, Steven J. J. Nigro, Ruth M. M. Hall
Summary: The aminoglycoside resistance gene aac(6')-Im is prevalent in the globally distributed resistant clones of Acinetobacter baumannii. It commonly coexists with the gene aacC1 in the global clone 2 (GC2) of A. baumannii. Understanding the dissemination and evolution of aac(6')-Im gene is important for tracking specific lineages and sublineages formation.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Carol H. Pong, Jade E. Peace, Christopher J. Harmer, Ruth M. Hall
Summary: In Gram-negative bacteria, the contribution of IS26 to the spread of antibiotic resistance and other genes is significant due to its unique mechanistic features, such as causing deletion of adjacent DNA segments and using two distinct reaction modes for cointegrate formation. The high frequency of the targeted conservative reaction mode is also crucial for its spread. However, the detailed mechanism of this reaction and the role of the RuvABC system in HJ resolution still need further investigation.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Matthew B. O'Rourke, Ben R. Roediger, Christopher J. Jolly, Ben Crossett, Matthew P. Padula, Phillip M. Hansbro
Summary: In this study, a hybrid bioinformatic/image-based method was developed for the identification and validation of viral biomarkers. This method leverages the power of mass spectrometry imaging and provides confident identifications for viral proteins without requiring MS/MS.
Article
Microbiology
Riti Mann, Rayane Rafei, Cindy Gunawan, Christopher J. Harmer, Mohammad Hamidian
Summary: Carbapenem resistance in Acinetobacter baumannii is primarily due to the global spread of two main clones that carry oxa23, oxa24, and oxa58. However, new carbapenem-resistant clones are emerging that are also resistant to a wide range of antibiotics.
MICROBIOLOGY SPECTRUM
(2022)