Article
Microbiology
Lucie Kuntova, Ivana Maslanova, Radka Oborilova, Hana Simeckova, Adela Finstrlova, Pavol Bardy, Marta Siborova, Liudmyla Troianovska, Tibor Botka, Petr Gintar, Ondrej Sedo, Zdenek Farka, Jiri Doskar, Roman Pantucek
Summary: This study describes a resistance mechanism targeting kayviruses that is encoded by a prophage. The mechanism belongs to a broader group of abortive infections, characterized by suicidal behavior of infected cells that are unable to produce phage progeny, thus ensuring the survival of the host population. Since the majority of staphylococcal strains are lysogenic, our findings are relevant for the advancement of phage therapy.
Article
Food Science & Technology
Sara Rodriguez-Sanchez, Ines Maria Ramos, Maria Rodriguez-Perez, Justa Maria Poveda, Susana Sesena, Maria Llanos Palop
Summary: The capacity of six lactic acid bacteria strains to decrease the growth of Staphylococcus aureus and the production of staphylococcal enterotoxins in co-cultured sheep's milk was evaluated. Three strains showed the highest decreases in viable cell counts and pH, mainly producing lactic acid and inhibiting the production of enterotoxins, suggesting their potential use as biocontrol agents against staphylococcal foodborne infections.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Cell Biology
Leszek Blicharz, Maciej Zochowski, Ksenia Szymanek-Majchrzak, Joanna Czuwara, Mohamad Goldust, Krzysztof Skowronski, Grazyna Mlynarczyk, Malgorzata Olszewska, Zbigniew Samochocki, Lidia Rudnicka
Summary: This study found that Staphylococcus aureus superantigens (SAgs) are associated with the severity of atopic dermatitis. The anterior nares and nonlesional skin may serve as reservoirs of SAg-positive S. aureus. Restoring the physiological microbiome can reduce the burden of SAgs and alleviate symptoms of atopic dermatitis.
Article
Multidisciplinary Sciences
Brandon A. Berryhill, Douglas L. Huseby, Ingrid C. McCall, Diarmaid Hughes, Bruce R. Levin
Summary: The study investigates the potential of the bacteriophage PYOSa for treating Staphylococcus aureus infections, showing promising results in terms of host range and killing rate. However, issues such as failure to clear bacterial populations and the emergence of small-colony variants need to be addressed before clinical application.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Sha Liu, Karen Hon, George Spyro Bouras, Alkis James Psaltis, Keith Shearwin, Peter-John Wormald, Sarah Vreugde
Summary: The isolated Staphylococcus aureus phages showed significant bactericidal activity against the biofilm form and a broad host range, with low mutation rate, indicating potential for further development as a treatment alternative to antibiotics for Methicillin-Resistant Staphylococcus Aureus (MRSA) infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Huixue Cai, Sijie Pei, Yan Zhang, Rongrong Liu, Shiling Lu, Baokun Li, Juan Dong, Qingling Wang, Xinrong Zhu, Hua Ji
Summary: This study constructed two models to evaluate the safety of Kazak cheese products, focusing on composition, S. aureus inoculation amount, Aw, fermentation temperature, and growth of S. aureus. The results indicate that fermentation temperature has the greatest influence on growth rates and lag times, followed by Aw and inoculation amount. A probability model was also developed to predict SE production, showing a high level of concordance with observed probabilities. This research provides valuable insights for manufacturers in determining optimal production parameters and preventing S. aureus growth and SE production.
Article
Virology
Matthew Brown, Alex Hall, Henriett Zahn, Marcia Eisenberg, Stephen Erickson
Summary: This study evaluated a cocktail of luciferase bacteriophage reporters for functionality in a matrix containing human serum and Staphylococcus aureus. Serum was found to inhibit the signal and decrease sensitivity, and immunoglobulin G was identified as an inhibitory component. Recombinant protein A improved the signal significantly.
Article
Food Science & Technology
Huixue Cai, Xiaomeng Kou, Hua Ji, Xin Wang, Haixia Wang, Yan Zhang, Shili Lu, Baokun Li, Juan Dong, Qingling Wang, Jing Zhou, Die Hu
Summary: The study revealed the presence of Staphylococcus aureus in Kazak cheese in Xinjiang, posing a potential threat to public health and highlighting the need for continuous monitoring.
Article
Biotechnology & Applied Microbiology
Rohini Krishna Kota, Harish Babu Kolla, Prakash Narayana Reddy, Naveen Kumar Kalagatur, Siva Kumar Samudrala
Summary: A novel multi-antigen subunit vaccine was designed, incorporating three protective antigens and validated through immunoinformatics analysis and computational approaches. The recombinant HAB protein (r-HAB) showed high immunogenicity in mice, inducing strong antibody responses and excellent protection against native toxins.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Jonathan Save, Yok-Ai Que, Jose M. Entenza, Camille Kolenda, Frederic Laurent, Gregory Resch
Summary: This study evaluated the potential of phage therapy for the treatment of Staphylococcus aureus infections and found that combining phages with a low-dose antibiotic could be a promising strategy for the treatment of S. aureus infective endocarditis.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Article
Chemistry, Physical
Baptiste Arbez, Marion Gardette, Christophe Gantzer, Neus Vila, Isabelle Bertrand, Sofiane El-Kirat-Chatel
Summary: This study investigates the adhesion of phage 187 on living Staphylococcus aureus cells using atomic force microscopy-based single-particle force spectroscopy. The addition of N-acetyl-D-glucosamine was found to decrease phage adhesion, suggesting its important role in phage 187 infection. The research also successfully identifies a major receptor required for phage 187 infection of S. aureus.
Article
Microbiology
Sindy Burgold-Voigt, Stefan Monecke, Anne Busch, Herbert Bocklisch, Sascha D. Braun, Celia Diezel, Helmut Hotzel, Elisabeth M. Liebler-Tenorio, Elke Mueller, Martin Reinicke, Annett Reissig, Antje Ruppelt-Lorz, Ralf Ehricht
Summary: This study describes a strain of Staphylococcus aureus isolated from a European badger, which belongs to ST425 and has a novel spa repeat sequence. It does not carry any resistance genes, but an uncommon enterotoxin gene see was detected in one of its three temperate bacteriophages.
Article
Microbiology
Yoona Lee, Bokyung Son, Yoyeon Cha, Sangryeol Ryu
Summary: Staphylococcus aureus is an important human pathogen that can be frequently encountered in clinical and food-processing surroundings. Bacteriophage PALS2, isolated from bird feces, belongs to the Myoviridae family and exhibits extended host inhibition. The genome of PALS2 is classified as a jumbo phage and contains genes involved in DNA replication, nucleotide metabolism, and RNA polymerase, suggesting its potential as a biocontrol agent.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Xiao-Yan Wang, Jian-Yu Yang, Yi-Ting Wang, Hui-Chao Zhang, Ming-Li Chen, Ting Yang, Jian-Hua Wang
Summary: A surface-enhanced Raman scattering (SERS) nanoprobe based on M13 phages was developed for selective detection and inactivation of Staphylococcus aureus. This method allowed for quantification of S. aureus in aqueous medium and showed promising potential for multifunctional platform for simultaneous detection and inactivation of S. aureus, including bactericidal capabilities.
Review
Food Science & Technology
Jing Zhang, Juan Wang, Jin Jin, Xin Li, Huiling Zhang, Xuening Shi, Chao Zhao
Summary: This study reviewed research on Staphylococcus aureus in milk and dairy products worldwide. The results showed that the prevalence of S. aureus in raw milk was higher than in dairy products and pasteurized milk. The resistance rates of penicillin and ampicillin were found to be high, and the antibiotic resistance of ampicillin, gentamicin, and chloramphenicol increased over time. The study also found a high prevalence of staphylococcal enterotoxins, with the highest rates associated with SEC and SEA genes. Improving the health of milking animals, elevating milking hygiene, and using pasteurization can ensure the hygiene and safety of raw milk.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Food Science & Technology
Gabriel Rocha Martins, Alvaro Ferreira Monteiro, Felipe Rafael Lopes do Amaral, Ayla Sant'Ana da Silva
Summary: The study successfully adapted and validated the FC method for quantifying total phenolics in acai seed extract, with a result of 38 g of pyrogallol equivalent/100 g of extract. The method showed linear response to the analyte, good selectivity, accuracy, precision, and robustness, with limits of detection and quantification determined. An additional step for tannins quantification based on selective precipitation was evaluated but found to reduce accuracy.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2021)
Article
Engineering, Chemical
Marcella Fernandes de Souza, Elba Pinto da Silva Bon, Ayla Sant'Ana da Silva
Summary: The study evaluated the on-site production of cellulases using steam-pretreated sugarcane bagasse as a carbon source, demonstrating its effectiveness in reducing costs and achieving a high enzyme yield. The enzyme preparation obtained was effective for hydrolyzing the sugarcane bagasse without the need for additional beta-glucosidases, supporting the integrated approach to decrease the cost of enzymatic hydrolysis of lignocellulosic biomass.
BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING
(2021)
Article
Polymer Science
Viviane P. Romani, Vilasia G. Martins, Ayla S. Silva, Paola C. Martins, Daiane Nogueira, Nadia Carbonera
Summary: The study shows that active and sustainable polymers for food packaging can be obtained from acai seeds and broken rice grains, turning them into valuable raw materials in the market. The incorporation of 5% acai seed flour in the films was found to be the most suitable, demonstrating antioxidant potential and better distribution in the film matrix. The packaging-related properties of the polymers were mainly influenced by the high-fiber content and polyphenolic compounds present in the acai seed flour.
JOURNAL OF APPLIED POLYMER SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Ronaldo Rodrigues de Sousa, Martina Costa Cerqueira Pinto, Erika Cristina Goncalves Aguieiras, Eliane Pereira Cipolatti, Evelin Andrade Manoel, Ayla Sant'Ana da Silva, Jose Carlos Pinto, Denise Maria Guimaraes Freire, Viridiana Santana Ferreira-Leitao
Summary: When selecting immobilized lipase for ester synthesis, factors such as cost, conversion rate, and reusability should be considered. Increasing the biocatalyst's loading can improve reusability of the lipase, but not in strict proportion, leading to a balance between the lowest biocatalyst loading and the lowest cost per batch.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2022)
Article
Chemistry, Physical
Ronaldo Rodrigues da Sousa, Ayla Sant'Ana da Silva, Roberto Fernandez-Lafuente, Viridiana Santana Ferreira-Leitao
Summary: The adoption of biocatalysis in solvent-free systems for ester production has been studied, with the development of a mathematical tool called SER for optimization of reaction conditions. The use of SER successfully predicted high conversion rates and allowed for reduction in the number of experiments and biocatalyst loadings.
Article
Biotechnology & Applied Microbiology
Roberta Pereira Espinheira, Vanessa Alves Lima Rocha, Tiago Martins Guimaraes, Catarina Amorim Oliveira, Marcella Fernandes De Souza, Gilberto B. Domont, Fabio Cesar Sousa Nogueira, Ricardo Sposina Sobral Teixeira, Elba Pinto Da Silva Bon, Ayla Sant'Ana Da Silva
Summary: Endoglucanases are crucial for high-solids enzymatic hydrolysis of lignocellulosic biomass, as they decrease medium viscosity and improve biomass liquefaction efficiency. This study evaluated the effectiveness of an endoglucanase-rich supernatant produced by a specific strain of Aspergillus awamori in speeding up biomass liquefaction reactions.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Applied
Daniel Oluwagbotemi Fasheun, Raul Alves de Oliveira, Elba P. S. Bon, Ayla Sant'Ana da Silva, Ricardo Sposina Sobral Teixeira, Viridiana Santana Ferreira-Leitao
Summary: The enzymatic hydrolysis of native starch can be enhanced by dry extrusion pretreatment, which reduces crystallinity and improves glucose yield. The study found that extruded CS:SB (1:0.25) had the highest glucose yield and hydrolysis efficiency.
CARBOHYDRATE POLYMERS
(2022)
Article
Chemistry, Applied
Gleicielle Tozzi Wurzler, Victor Teixeira da Silva, Debora de Almeida Azevedo, Ayla Sant' Ana da Silva, Fabio Bellot Noronha
Summary: This study investigated the fractionation of sugarcane bagasse using Organosolv treatment and different catalysts. The results showed that the catalysts had different effects on the delignification and product distribution. Mo2C/AC and Mo2C/Al2O3 catalysts showed higher bio-oil yield and a solid residue that was more easily hydrolyzed by cellulases.
FUEL PROCESSING TECHNOLOGY
(2022)
Article
Energy & Fuels
Fernanda Thimoteo Azevedo Jorge, Ayla Sant'Ana da Silva, George Victor Brigagao
Summary: This study evaluates the viability and impacts of four alternatives for utilizing acai seeds waste in the Brazilian Amazon. The option of co-production of polyphenols and electricity shows good economic and environmental performances, while complete combustion for power generation is the least profitable.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Biochemical Research Methods
Domingos F. M. Neto, Jose R. S. Nascimento, Gabriel R. Martins, Ayla S. Silva, Gilberto B. Domont, Francisco A. P. Campos, Fabio C. S. Nogueira
Summary: Acai seeds are a rich source of mannans and polyphenols. Proteome analysis revealed enzymes involved in the biosynthesis and degradation of mannans and polyphenols, providing insights into the nutritional value and potential applications of acai seeds.
Article
Environmental Sciences
Bruno Cesar S. Coelho, Davi M. M. C. da Silva, Edward D. Hermosilla, Ricardo S. S. Teixeira, Elba P. S. Bon, Maria Cristina Diez, Viridiana S. Ferreira-Leitao, Ayla Sant'Ana da Silva
Summary: This study investigated the effects of a biological pretreatment (BPT) on the efficiency of hydrothermal pretreatment (HPT) for enzymatic hydrolysis of sugarcane straw. The results showed that Pleurotus ostreatus was the most effective microorganism for BPT, and combining BPT with HPT improved the glucose yield. The findings suggest that BPT is a useful aid for the HPT pretreatment of lignocellulosic biomass.
WASTE AND BIOMASS VALORIZATION
(2022)
Article
Agriculture, Multidisciplinary
Gabriel R. Martins, Mariana M. G. Mattos, Fabiane Marques Nascimento, Felipe L. Brum, Ronaldo Mohana-Borges, Natalia Guimaraes Figueiredo, Domingos F. M. Neto, Gilberto Barbosa Domont, Fabio Cesar Sousa Nogueira, Francisco de Assis de Paiva Campos, Ayla Sant'Ana da Silva
Summary: Changes in the phenolic profile and antioxidant properties were investigated in developing acai seeds. Earlier stages displayed higher antioxidant activity and polyphenols content, with procyanidins identified as the major components. Extracted procyanidins decreased in length along seed development, with (-)-epicatechin being the predominant unit and (+)-catechin increasing in presence. This variation was correlated to the abundance of key enzymes for procyanidin biosynthesis.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemical Research Methods
Felipe Lopes Brum, Gabriel R. R. Martins, Ronaldo Mohana-Borges, Ayla Sant'Ana da Silva
Summary: An adapted sample preparation method was developed for analyzing acai seeds using MALDI-IMS, which involved softening, cutting, softening again, sectioning, and storing the samples. This method enable the analysis of the spatial distribution of carbohydrates in the endosperm of acai seeds.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2023)
Article
Chemistry, Physical
Ronaldo Rodrigues de Sousa, Rui de Paula Vieira de Castro, Nadinne Medeiros Assis, Ayla Sant'Ana da Silva, Denise Maria Guimaraes Freire, Roberto Fernandez-Lafuente, Viridiana Santana Ferreira-Leitao
Summary: Although significant advances have been made in the biocatalytic synthesis of esters, few industrial plants worldwide currently produce esters using biocatalysts. Biocatalytic routes can provide green and sustainable esters with operational advantages over conventional syntheses. However, most literature neglects or generically describes the economic issues, and scaling-up studies are scarce. The cost of immobilized lipases, the main disadvantage of biocatalysis, has not been studied as extensively as other technical aspects. Preliminary techno-economic assessments are crucial to overcome the economic drawbacks and promote wider application of biocatalytic ester syntheses in large-scale contexts.
Review
Chemistry, Physical
Ronaldo Rodrigues Sousa, Ayla Sant'Ana Silva, Roberto Fernandez-Lafuente, Viridiana Santana Ferreira-Leitao
Summary: Esters are important compounds in industry, and using biocatalysis for their synthesis in solvent-free systems offers economic and environmental advantages. However, optimizing reactions in these systems is challenging due to the complexity and variety of phenomena involved.
CATALYSIS SCIENCE & TECHNOLOGY
(2021)