Article
Chemistry, Analytical
Xu Chen, Wei Li, Yue Ma
Summary: Based on the 16S rRNA gene, a competitive annealing-mediated isothermal amplification (CAMP) method is developed to reduce the risk of C. sakazakii infection. The CAMP method accurately identifies the target strains and does not yield false positive results with non-target bacterial strains. Accelerating primers are designed to enhance amplification efficiency, and hydroxynaphthol blue is employed as a visual indicator for simplification.
ANALYTICAL LETTERS
(2023)
Article
Chemistry, Analytical
Xu Chen, Yue Ma, Shuangyu Miao, Dongnan Li, Ye Zhang
Summary: In this study, a multiplex competitive annealing mediated isothermal amplification (mCAMP) assay on a microfluidic chip was developed for the visual detection of Cronobacter sakazakii in powdered infant formula (PIF) samples. The assay showed high specificity and a limit of detection of 2.2 x 10(3) CFU g(-1). The results are visible to the naked eye, making it suitable for rapid analysis in resource-limited settings.
Article
Microbiology
Alfred Ke, Valeria R. Parreira, Jeffrey M. Farber, Lawrence Goodridge
Summary: The study finds that the combination of probiotics and prebiotics can inhibit the contamination of Cronobacter sakazakii in powdered infant formula (PIF), preventing severe illnesses in infants. The combination may inhibit the growth of C. sakazakii through the production of antimicrobial metabolites, increasing species diversity, or a combination of mechanisms. Additionally, the native gut microbiota may also play a role in inhibiting C. sakazakii.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Sahar M. Kamal, Nagah M. S. Maharik, Antonio Valero, Alshimaa M. Faried
Summary: The occurrence of Cronobacter spp. in table eggs and egg-based desserts obtained from retail stores was investigated. Cronobacter was detected in 31.7% of the examined samples, with a higher prevalence in farm eggs compared to other types of eggs and desserts. The predominant isolates were C. sakazakii and C. muytjensii, with a higher incidence in small-scale ice cream.
ITALIAN JOURNAL OF FOOD SCIENCE
(2023)
Article
Multidisciplinary Sciences
Mikhlid H. Almutairi, Mohamed M. Helal
Summary: This study isolated thirty-seven marine bacterial isolates from Haqel Beach in Tabuk, Saudi Arabia, with only nine of them capable of producing extracellular polysaccharides. The best producer was identified as an Enterobacter species through morphological, biochemical, and molecular characterization. By optimizing culture fermentation conditions, a high EPS yield of 8.6 g/l was achieved, with optimal medium compositions and incubation parameters established.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2021)
Article
Microbiology
Sambit K. Das, Sanath H. Kumar, Binaya B. Nayak, Manjusha Lekshmi
Summary: This study investigated the incidence of Cronobacter spp. in seafood from retail fish markets in Mumbai, India. It found the highest incidence of Cronobacter spp. in dried fish, with Cronobacter malonaticus being the most common species identified. The detection of Cronobacter spp. highlights the importance of monitoring this emerging pathogen in tropical seafood.
CURRENT MICROBIOLOGY
(2021)
Article
Parasitology
Qian Zhang, Shumin Wang, Xinyu Zhang, Kexin Zhang, Wenjuan Liu, Ruiling Zhang, Zhong Zhang
Summary: This study found that adding Enterobacter hormaechei can change the gut microbial community of housefly larvae, promote the reproduction of beneficial bacteria, and inhibit the growth of harmful bacteria, providing new possibilities for housefly control.
PARASITES & VECTORS
(2021)
Article
Biotechnology & Applied Microbiology
L. M. P. S. Braga, B. A. A. Saad, C. T. F. de Oliveira, C. E. Volpe-Chaves, M. L. G. G. Lacerda, S. J. Forsythe, J. Venturini, S. M. V. L. de Oliveira, A. M. M. Paniago, L. da Costa, R. V. S. L. de Miranda, C. M. F. dos Reis, M. L. L. Brandao
Summary: This study investigated an uncertain species identification for an infection located on a catheter tip of a patient with Covid-19. The strain, initially identified as Cronobacter spp. by VITEK (R) 2, was later confirmed to be Curtobacterium spp. through polyphasic analysis. The misidentification occurred due to the Gram-variable characteristic of the strain. Additional techniques are required for species-level identification.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Plant Sciences
Naweena Thapa, Michelle D. Danyluk, Kayla M. Gerberich, Evan G. Johnson, Megan M. Dewdney
Summary: The study focused on the importance of thermotherapy to eliminate the Huanglongbing bacterium for citrus production, showing that high temperatures and long durations of treatment can effectively reduce the presence of the pathogen.
Article
Multidisciplinary Sciences
Mikhlid H. Almutairi, Mohamed M. Helal
Summary: The research team isolated Enterobacter sp. from the marine environment and produced a high yield of EPS, with its monosaccharide composition and sulfation modification enhancing its antibacterial activity against Staphylococcus aureus and Escherichia coli. However, the EPS showed no significant antifungal activity against Candida albicans and did not exhibit prebiotic activities. Further research and adaptation are needed for practical and industrial applications.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2021)
Article
Plant Sciences
Gautam Anand, Vijay Laxmi Shrivas, Shubham Dubey, Annapurna Bhattacharjee, Shilpi Sharma
Summary: The global food crisis has driven the agricultural sector to evolve rapidly to meet demand. Understanding the interactions in the soil microbiome is crucial for sustainable agricultural development. This study aimed to demonstrate the stress-alleviating effects of indigenous ACC deaminase producing bacteria in Cajanus cajan rhizosphere, and their influence on the rhizospheric microbiome.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Microbiology
Veronika Merold, Kim Silberreis, Kilian Stoecker
Summary: Real-time PCR has become an essential tool in clinical microbiology and key performance indicators of rtPCR assays include specificity, analytical and clinical sensitivity. This study demonstrated the use of naturally pre-amplified ribosomal RNA as a target for highly sensitive rtPCR detection of Yersinia pestis and Francisella tularensis, showing superior performance compared to previous assays. The assays can be applied on heat-inactivated samples without the need of time-consuming nucleic acid extraction, which may have potential for rapid pathogen diagnostics.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Julieta Salgueiro, A. Laura Nussenbaum, Fabian H. Milla, Elias Asimakis, Lucia Goane, M. Josefina Ruiz, Guillermo E. Bachmann, Maria T. Vera, Panagiota Stathopoulou, Kostas Bourtzis, Ania T. Deutscher, Silvia B. Lanzavecchia, George Tsiamis, Diego F. Segura
Summary: The gut bacterial community of Anastrepha fraterculus sp. 1 larvae was studied using Next Generation Sequencing. The community composition was affected by the host species and geographic origin. There were differences in the bacterial profiles among trees of the same species and location at smaller spatial scales. Fruit size did not have an effect on the larval gut bacteriome.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biodiversity Conservation
Yun-He Wu, Parinya Pawangkhanant, Jin-Min Chen, Wei Gao, Chatmongkon Suwannapoom, Jing Che
Summary: Thailand is considered a global biodiversity hotspot with a striking diversity of endemic species. However, research studies have shown that the level of amphibian diversity in the country has been significantly underestimated. The discovery of Leptobrachella ventripunctata species reaffirms that the diversity within the genus has been underestimated, highlighting a lack of existing knowledge on amphibian taxonomy and biodiversity in Thailand's national border areas.
BIODIVERSITY DATA JOURNAL
(2021)
Article
Marine & Freshwater Biology
Alyaa Elsaid Abdelaziz Fadl, Shuhei Yamaguchi, Kaori Wakabayashi
Summary: Through a combination of molecular and morphological analyses, this study successfully linked wild-caught larval specimens from the Seto Inland Sea to a certain species and provided a detailed morphological description of the last larval stage. The results highlighted the importance of integrating molecular and morphological approaches in solving stomatopod larval identification issues.
JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM
(2021)