Article
Biotechnology & Applied Microbiology
Emily E. Jackson, Nathalie Gnanou Besse, Rabeb Bennour Hennekinne, Timothy Ells, Michel Laurentie, Thomas Meheut, Stephen J. Forsythe
Summary: This study reports the sequence types of Cronobacter sakazakii strains and investigates the correlation between sequence type and growth rate. The results suggest that there is no strong correlation between the two factors.
Article
Food Science & Technology
Hongxuan Li, Shiqian Fu, Danliangmin Song, Xue Qin, Wei Zhang, Chaoxin Man, Xinyan Yang, Yujun Jiang
Summary: In this study, 35 Cronobacter strains isolated from powdered infant formula and its processing environment were identified and typed using 16S rRNA sequencing and multilocus sequence typing (MLST) technology. A total of 35 different sequence types were obtained, including three new ones that were isolated for the first time. The antibiotic resistance analysis revealed that all isolates were resistant to erythromycin but sensitive to ciprofloxacin. Moreover, 68.57% of the strains showed multi-drug resistance, with the most drug-resistant Cronobacter strain exhibiting resistance to 13 different drugs. Transcriptomics analysis identified 77 differentially expressed genes related to drug resistance, and the study further revealed that Cronobacter strains can activate multidrug efflux systems to enhance drug resistance. Understanding the drug resistance and mechanisms of Cronobacter is of great public health significance for the rational selection and development of antibacterial drugs, as well as the control and treatment of infections caused by this pathogenic microorganism.
Article
Food Science & Technology
Hua Jiang, Yue Xiang, Xiaojuan He, Chencheng Li, Fuxing Lin, Jihong Shao, Yuanhong Li
Summary: This study investigated the prevalence and characterization of Cronobacter spp. in dried edible mushrooms in Jiangsu province, China. The overall prevalence of Cronobacter spp. in dried edible mushrooms was 14.8%, with high genetic diversity observed. Some Cronobacter strains showed resistance to certain antibiotics, although they were susceptible to most tested antibiotics. Most isolates showed weak biofilm formation ability. This study provides new epidemiological evidence for the presence of Cronobacter spp. in dried edible mushrooms.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jen-Fu Hsu, Jang-Jih Lu, Chih Lin, Shih-Ming Chu, Lee-Chung Lin, Mei-Yin Lai, Hsuan-Rong Huang, Ming-Chou Chiang, Ming-Horng Tsai
Summary: In this study, a new approach using CRISPR and RFLP analyses was utilized to study 103 CC17/III GBS isolates causing neonatal invasive diseases. It was found that GBS isolates with different CRISPR-RFLP patterns exhibited significant differences in occurrences of severe sepsis and meningitis. These findings suggest that a specific CRISPR-RFLP pattern could potentially be used as a new biomarker to predict meningitis and illness severity in CC17/III GBS infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Jingxian Liu, Jing Zhao, Chencui Huang, Jingxu Xu, Wei Liu, Jiajia Yu, Hongyan Guan, Ying Liu, Lisong Shen
Summary: This study aimed to construct a practical and facile clonal complex (CCs) prediction model for S. agalactiae. Through molecular classification and feature analysis of 325 GBS strains, a model that accurately predicts CCs was established, and it was found that the model based on serotype and antibiotic resistance performed well.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2022)
Article
Food Science & Technology
Qifan Sun, Jianheng Cheng, Ruoqin Lin, Jingyi Li, Ying Zhang, Xinwen Liang, Yue Su, Rui Pang, Liang Xue, Haiyan Zeng, Qihui Gu, Yu Ding, Qingping Wu, Moutong Chen, Jumei Zhang
Summary: Listeria monocytogenes is a globally important foodborne pathogen, and the emergence of clonal complex (CC) 87 and CC88 strains poses a threat to human health. This study developed a multiplex PCR technology that can simultaneously detect CC87 and CC88 strains, showing high specificity and sensitivity. The application of this technology in artificially contaminated milk and food samples is effective, and it is important for monitoring the prevalence of strains and evaluating the effectiveness of disinfection measures.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Ichiro Imanishi, Keita Iyori, Akira Take, Ryota Asahina, Manami Tsunoi, Ryuji Hirano, Jumpei Uchiyama, Yoichi Toyoda, Yoshihiko Sakaguchi, Shunji Hayashi
Summary: Although antibiotic-resistant strains of S. canis are isolated at a high rate, they can currently be treated with beta-lactamase-inhibiting penicillins. CC13 may be a pathogenic cluster with high levels of antibiotics resistance.
BMC VETERINARY RESEARCH
(2022)
Article
Infectious Diseases
Ying Wang, Xin Wan, Li Zhao, Peipei Jin, Ju Zhang, Xin Zhou, Naifang Ye, Xiaowei Wang, Yaping Pan, Liangfei Xu
Summary: This study aimed to investigate the clonality of clinical Candida tropicalis isolates from normally sterile body fluids and explore the clonal complexes in Hefei, China. Through multilocus sequence typing and molecular phylogenetic analysis, a local prevalent fluconazole-resistant clonal complex was identified, which showed a close relationship with the genetic background.
Article
Microbiology
Hannah Bolinger, William G. Miller, Jason A. Osborne, Jeffrey Niedermeyer, Sophia Kathariou
Summary: Houseflies may act as vectors for the dissemination of Campylobacter, including multidrug-resistant strains, within turkey houses, and potentially between different turkey houses and farms in the same region.
Article
Microbiology
Jen-Fu Hsu, Yu-Ning Chen, Shih-Ming Chu, Wei-Ju Lee, Hsuan-Rong Huang, Ming-Chou Chiang, Peng-Hong Yang, Ming-Horng Tsai, Jang-Jih Lu
Summary: A type lb ST12 GBS strain is associated with a significantly higher risk of severe sepsis, higher illness severity, and a significantly higher rate of sepsis-attributable mortality. The presence of an ICESag37 gene cluster and specific pilus genes carrying multiple antimicrobial resistance and virulence genes may be responsible for the clinical characteristics. Continuous monitoring of the type lb ST12 GBS strain is warranted in the future.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Fardousi Akter Sathi, Meiji Soe Aung, Shyamal Kumar Paul, Syeda Anjuman Nasreen, Nazia Haque, Sangjukta Roy, Salma Ahmed, Mohammad Monirul Alam, Shahed Khan, Mohammad Arif Rabbany, Joy Prokas Biswas, Nobumichi Kobayashi
Summary: In this study, the existing multilocus sequence typing (MLST) scheme for Candida auris was modified and MLST schemes were developed for Candida blankii and Kodamaea ohmeri. These schemes were successfully applied to clinical isolates from Bangladesh in 2021, revealing the clonal diversity of these three fungal species.
Article
Infectious Diseases
Chih-Chen Kao, Chi-Hsiang Lai, Min-Yi Wong, Tsung-Yu Huang, Yuan-Hsi Tseng, Chu-Hsueh Lu, Chien-Chao Lin, Yao-Kuang Huang
Summary: Patients receiving hemodialysis are susceptible to vascular access infections caused by Staphylococcus aureus (S. aureus), which increased during the COVID-19 pandemic. This study investigated the changes in the molecular and antibiotic resistance profiles of S. aureus isolates from vascular access infections. The prevalence and clone distribution of S. aureus isolates varied between arteriovenous grafts (AVGs) and tunneled cuffed catheters (TCCs) before and during the pandemic.
Article
Infectious Diseases
Eun-Young Kim, Si Hyun Kim, Gyu Ri Kim, Jeong Hwan Shin
Summary: This study evaluated clonal complex changes of levofloxacin-resistant S. pneumoniae strains and investigated the relationship between levofloxacin resistance and pneumococcal serotypes. Most strains were multidrug resistant and nonvaccine serotypes. Additionally, there were changes in the clonal complexes of the strains.
MICROBIAL DRUG RESISTANCE
(2022)
Article
Microbiology
Meiji Soe Aung, Asami Matsuda, Noriko Urushibara, Mitsuyo Kawaguchiya, Nobuhide Ohashi, Norifumi Matsuda, Mayumi Nakamura, Masahiko Ito, Satoshi Habadera, Akinori Matsumoto, Mina Hirose, Nobumichi Kobayashi
Summary: The study analyzed the clonal diversity and toxin gene prevalence in clinical isolates of Clostridium perfringens from humans in northern Japan. The most frequent toxinotype was A, followed by F and G, with additional toxin genes identified in a small number of isolates. Various toxin gene profiles were observed, indicating the presence of six clonal complexes and showing diversity in C. perfringens clones of human origin.
Article
Infectious Diseases
Kohei Ogura, Daiki Kaji, Masakazu Sasaki, Yoshihito Otsuka, Norihiko Takemoto, Tohru Miyoshi-Akiyama, Ken Kikuchi
Summary: The CC1/t1784 clone of MRSA in Japan has rapidly expanded without genomic changes.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Microbiology
Charlotte K. Hind, Christopher G. Dowson, J. Mark Sutton, Thomas Jackson, Melanie Clifford, R. Colin Garner, Lloyd Czaplewski
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2019)
Article
Biochemistry & Molecular Biology
Dom Bellini, Lizbe Koekemoer, Hector Newman, Christopher G. Dowson
JOURNAL OF MOLECULAR BIOLOGY
(2019)
Article
Chemistry, Multidisciplinary
Anita C. Catherwood, Adrian J. Lloyd, Julie A. Tod, Smita Chauhan, Susan E. Slade, Grzegorz P. Walkowiak, Nicola F. Galley, Avinash S. Punekar, Katie Smart, Dean Rea, Neil D. Evans, Michael J. Chappell, David Roper, Christopher G. Dowson
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Multidisciplinary Sciences
Laura C. Jacques, Stavros Panagiotou, Murielle Baltazar, Madikay Senghore, Shadia Khandaker, Rong Xu, Laura Bricio-Moreno, Marie Yang, Christopher G. Dowson, Dean B. Everett, Daniel R. Neill, Aras Kadioglu
NATURE COMMUNICATIONS
(2020)
Article
Biotechnology & Applied Microbiology
P. V. Costa, R. M. de Siqueira, A. C. Rosa Guimaraes, L. Vasconcellos, V. Midlej, G. M. Silva da Conceicao, S. J. Forsythe, M. L. Lima Brandao
Summary: This study evaluated the cytotoxic activity of Cronobacter strains isolated from foods and clinical samples in Brazil, and identified some strains capable of producing cytotoxins.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Food Science & Technology
Na Ling, Yujun Jiang, Haiyan Zeng, Yu Ding, Stephen Forsythe
Summary: This review discusses the progress made in China regarding the research on Cronobacter, including large-scale surveys and the adoption of DNA sequence-based methods for identification and genotyping of the organism. These methods have significantly advanced understanding of the taxonomy, ecology, and virulence of Cronobacter, leading to improved source tracking in infant formula production facilities and epidemiological investigations.
JOURNAL OF FOOD SCIENCE
(2021)
Article
Chemistry, Medicinal
J. Cama, R. Leszczynski, P. K. Tang, A. Khalid, V Lok, C. G. Dowson, A. Ebata
Summary: The COVID-19 pandemic has highlighted the dangers of infectious diseases globally, impacting both global health and the world economy. However, the even greater long-term danger of antimicrobial resistance in pathogenic bacteria and fungi is still under-appreciated. There is a critical need for governments to incentivize the development of antimicrobials to address this significant global challenge.
ACS INFECTIOUS DISEASES
(2021)
Article
Multidisciplinary Sciences
Surya D. Aggarwal, Adrian J. Lloyd, Saigopalakrishna S. Yerneni, Ana Rita Narciso, Jennifer Shepherd, David Roper, Christopher G. Dowson, Sergio R. Filipe, N. Luisa Hiller
Summary: In response to unfavorable conditions, the Gram-positive pathogen Streptococcus pneumoniae relies on cell wall biosynthesis proteins MurM and MurN. MurM modulates stress response by decreasing the pool of mischarged tRNAs, while enhanced lysis in the absence of MurM may be caused by LytA.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Anna York, Adrian J. Lloyd, Charo del Genio, Jonathan Shearer, Karen J. Hinxman, Konstantin Fritz, Vilmos Fulop, Christopher G. Dowson, Syma Khalid, David Roper
Summary: The study explored the interaction between MurM protein and membrane phospholipids, revealing that the activity of MurM is regulated by specific phospholipids. The spatial association of pneumococcal membrane phospholipids with MurM activity may play a critical role in the expression of penicillin resistance.
Review
Agriculture, Dairy & Animal Science
Lihan Wang, Stephen J. Forsythe, Xinyan Yang, Shiqian Fu, Chaoxin Man, Yujun Jiang
Summary: Cronobacter genus includes food-borne pathogens that can cause severe infections in infants, with a high mortality rate. Advanced detection technology and analysis of high-frequency species from different sources can aid in targeted control of Cronobacter. The bacteria's resistance to environmental stresses and the development of new control techniques highlight the importance of strict monitoring and prevention throughout food processing and storage.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Chemistry, Medicinal
Hector Newman, Alen Krajnc, Dom Bellini, Charles J. Eyermann, Grant A. Boyle, Neil G. Paterson, Katherine E. McAuley, Robert Lesniak, Mukesh Gangar, Frank von Delft, Jurgen Brem, Kelly Chibale, Christopher J. Schofield, Christopher G. Dowson
Summary: The research reveals that boron-containing compounds can form various complexes with PBP3, showing the potential for the development of new classes of boron-based antibiotics that are not affected by beta-lactamase-driven resistance.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Medicinal
Wendy Tran, Ali S. Kusay, Paige M. E. Hawkins, Chen-Yi Cheung, Gayathri Nagalingam, Venugopal Pujari, Daniel J. Ford, Alexander Stoye, Jessica L. Ochoa, Rebecca E. Audette, Elinor Hortle, Stefan H. Oehlers, Susan A. Charman, Roger G. Linington, Eric J. Rubin, Christopher G. Dowson, David Roper, Dean C. Crick, Thomas Balle, Gregory M. Cook, Warwick J. Britton, Richard J. Payne
Summary: The study reports the design and synthesis of inhibitors targeting Mtb MurX enzyme, showing potent antimicrobial activity against Mycobacterium tuberculosis. These inhibitors have the potential to be developed into new drugs for tuberculosis treatment.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Carmina Micelli, Yunfei Dai, Nicole Raustad, Ralph R. Isberg, Christopher G. Dowson, Adrian J. Lloyd, Edward Geisinger, Allister Crow, David I. Roper
Summary: Acinetobacter baumannii is a gram-negative bacterial pathogen that causes challenging nosocomial infections. The study reveals the molecular details of cell wall elongation inhibition upon zinc starvation in A. baumannii. The findings offer a perspective for dual inhibition of zinc-dependent peptidoglycan synthases and metallo-beta-lactamases by metal-chelating agents.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Plant Sciences
Amanda J. Dowson, Adrian J. Lloyd, Andrew C. Cuming, David Roper, Lorenzo Frigerio, Christopher G. Dowson
Summary: Accumulating evidence suggests that peptidoglycan is synthesized around the chloroplasts of many photosynthetic eukaryotes, but the biosynthetic pathway has not been demonstrated. This study employed mass spectrometry and enzymology to characterize the synthesis of peptidoglycan in the moss Physcomitrium (Physcomitrella) patens and found that the biosynthetic pathway is derived from a Gram-negative bacterial progenitor of chloroplasts in early-diverging land plants.
Article
Chemistry, Multidisciplinary
Angelo Frei, Johannes Zuegg, Alysha G. Elliott, Murray Baker, Stefan Braese, Christopher Brown, Feng Chen, Christopher G. Dowson, Gilles Dujardin, Nicole Jung, A. Paden King, Ahmed M. Mansour, Massimiliano Massi, John Moat, Heba A. Mohamed, Anna K. Renfrew, Peter J. Rutledge, Peter J. Sadler, Matthew H. Todd, Charlotte E. Willans, Justin J. Wilson, Matthew A. Cooper, Mark A. T. Blaskovich