Article
Immunology
Gokhlesh Kumar, Arun Sudhagar, Saloni Shivam, Frank Nilsen, Jerri L. Bartholomew, Mansour El-Matbouli
Summary: In this study, 136 immunogenic proteins induced by T. bryosalmonae during infection in brown trout were identified. These proteins play important roles in signal transduction, metabolism, and other processes, and can provide insights into the pathogenesis of T. bryosalmonae in susceptible hosts.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hao Lu, Xiaodan Li, Gaoyan Wang, Chenchen Wang, Jiajia Feng, Wenjia Lu, Xiangru Wang, Huanchun Chen, Manli Liu, Chen Tan
Summary: Baicalein, in combination with ampicillin, effectively improved severe Streptococcus suis infection by inhibiting the hemolytic activity of SLY and providing protection in infected cells. Baicalein showed potential as a new therapeutic strategy for STSLS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ting Gao, Yiqing Tan, Yanjun Wang, Fangyan Yuan, Zewen Liu, Keli Yang, Wei Liu, Rui Guo, Chang Li, Yongxiang Tian, Danna Zhou
Summary: Theaflavin (TF1), a compound extracted from black tea, was found to have antibacterial and antihemolytic activity against Streptococcus suis (S. suis). It showed inhibitory effects on S. suis growth, hemolytic activity, and biofilm formation. TF1 also improved the survival rate of S. suis-infected mice and reduced bacterial load and inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Veterinary Sciences
Yi Lu, Fa Xiang, Liuyi Xu, Hongliang Tian, Qi Tao, Kaixiang Jia, Hang Yin, Chao Ye, Rendong Fang, Lianci Peng
Summary: This study investigates the bactericidal and anti-inflammatory effects of chicken cathelicidin-1 (CATH-1) against SS2 infection. The results show that CATH-1 has a better bactericidal effect compared to other species' cathelicidins and can destroy bacterial morphology and affect bacterial ultrastructure. Additionally, CATH-1 reduces the inflammatory response through direct neutralization and by regulating the TLR2/4/NF-κB/ERK pathway. This study provides a scientific basis for the research and development of antimicrobial peptides as new antimicrobial agents.
VETERINARY RESEARCH
(2023)
Article
Microbiology
Shujie Wang, Gang Wang, Yan-Dong Tang, Siqi Li, Lei Qin, Menghang Wang, Yong-Bo Yang, Marcelo Gottschalk, Xuehui Cai
Summary: This study reveals the mechanisms underlying splenic lesions caused by Streptococcus suis infection, including apoptosis and activation of inflammatory responses. The findings provide new insights into the pathogenesis of S. suis.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Xianfeng Hui, Zhongmin Xu, Lei Cao, Liang Liu, Xian Lin, Yong Yang, Xiaomei Sun, Qiang Zhang, Meilin Jin
Summary: The protein HP0487 of Streptococcus suis serotype 2 was found to play a significant role in adhesion and anti-phagocytosis to neutrophils, influencing the pathogenicity and virulence of SS2.
VETERINARY MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Quan Li, Guodong Zhou, Xia Fei, Yichen Tian, Shifeng Wang, Huoying Shi
Summary: Bacterial outer membrane vesicles (OMVs) are a promising vaccine platform due to their adjuvanticity properties. However, the location and amount of heterologous antigen expression in OMVs delivered by genetic engineering strategies need to be optimized.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Roel M. van Harten, Johanna L. M. Tjeerdsma-van Bokhoven, Astrid de Greeff, Melanie D. Balhuizen, Albert van Dijk, Edwin J. A. Veldhuizen, Henk P. Haagsman, Maaike R. Scheenstra
Summary: The study evaluates the prophylactic capacity of D-CATH-2 and its derivatives in mammalian cells, and finds that they possess strong antibacterial capacity and immunomodulatory function. Administering D-C(1-21) provides partial protection against S. suis infection and reduces disease severity and mortality.
JOURNAL OF ADVANCED RESEARCH
(2022)
Article
Biophysics
Kanjana Kunpatee, Kannika Khantasup, Kittinan Komolpis, Abdulhadee Yakoh, Suphachai Nuanualsuwan, Mohini M. Sain, Sudkate Chaiyo
Summary: This study developed an electrochemical lateral flow immunoassay (eLFIA) strip with high reproducibility for rapid and accurate detection of Streptococcus suis serotype 2. The strip integrated ratiometric electrochemical detection and LFIA, resulting in improved reproducibility compared to a single electrode. The biosensor showed good linearity and a low limit of detection, and was successfully applied in human serum samples.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Lu Wang, Jing Sun, Jiyu Zhao, Jieyu Bai, Yueling Zhang, Yao Zhu, Wanjiang Zhang, Chunlai Wang, Paul R. Langford, Siguo Liu, Gang Li
Summary: In this study, a high-fidelity detection and serotyping platform for Streptococcus suis serotype 2 was developed based on recombinase polymerase amplification (RPA) and a CRISPR-Cas12a system. The platform showed accurate and rapid detection with a detection limit of 10 CFU and the ability to differentiate serotypes. It is a suitable method for point-of-care detection.
Article
Immunology
Mei-Fang Tan, Jia Tan, Fan-Fan Zhang, Hai-Qin Li, Hua-Yuan Ji, Shao-Pei Fang, Cheng-Cheng Wu, Yu-Ling Rao, Yan-Bin Zeng, Qun Yang
Summary: This study investigated the pleiotropic effects of exogenous glycogen on Streptococcus suis serotype 2 (SS2) through transcriptome sequencing. The results showed that glycogen induction significantly influenced the metabolic pathways and virulence-associated factors of SS2. Extracellular glycogen utilization enhanced the hemolytic activity, adhesion and invasion ability, and pathogenicity of SS2. These findings are important for understanding the adaptation and pathogenicity mechanisms of SS2.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Chaoxiong Yue, Chenlu Hu, Peng Xiang, Siming Zhang, Hongde Xiao, Wei Zhou, Hui Jin, Deshi Shi, Jinquan Li, Lang Xu, Yushan Chen, Yan Zeng
Summary: Streptococcus suis (S. suis) is a significant swine and human pathogen that can cause severe meningitis with high mortality. This study demonstrates that autophagy serves as a cellular defense mechanism in microglial cells following S. suis infection, aiding in limiting bacterial invasion and microglial inflammation.
VETERINARY MICROBIOLOGY
(2021)
Article
Microbiology
Anusak Kerdsin, Rujirat Hatrongjit, Thidathip Wongsurawat, Piroon Jenjaroenpun, Peechanika Chopjitt, Parichart Boueroy, Nahuel Fittipaldi, Han Zheng, Marcelo Gottschalk
Summary: Streptococcus suis is a zoonotic pathogen that can cause invasive infections in both humans and pigs, with different serotypes leading to distinct clinical manifestations and infection characteristics. Some strains exhibit antimicrobial resistance while lacking specific virulence-associated genes.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Li Yi, Qingying Fan, Yuxin Wang, Chenlong Mao, Jinpeng Li, Manyu Jin, Xiaoling Zhang, Ke Ding, Yang Wang
Summary: The study demonstrated that recombinant PDH protein induced high levels of antibodies in mice, enhancing the immune response and protecting pathological organs from damage. Additionally, PDH antiserum significantly inhibited the growth of S. suis and the formation of biofilm in vitro.
VETERINARY MICROBIOLOGY
(2021)
Article
Microbiology
Servane Payen, David Roy, Anais Boa, Masatoshi Okura, Jean-Philippe Auger, Mariela Segura, Marcelo Gottschalk
Summary: The study evaluated the role of Lgt and Lsp enzymes in the pathogenesis of Streptococcus suis serotype 2 infections. Lack of these enzymes led to a significant reduction in S. suis' ability to activate phagocytic cells and induce pro-inflammatory mediators.
Article
Chemistry, Analytical
Lanlan Xu, Hongyu Chu, Dejiang Gao, Qiong Wu, Ying Sun, Zhenxin Wang, Pinyi Ma, Daqian Song
Summary: Fluorescence imaging-guided diagnostics is a promising method for detecting tumors in situ due to its simplicity and non-invasiveness. The chemosensor LAN-beta gal, with a specific recognition unit of d-galactose residue and a fluorophore of LAN-OH, showed remarkable fluorescence enhancement after beta-gal was digested. It successfully visualized endogenous beta-gal, distinguished ovarian cancer cells from normal cells, and was applied in vivo for tumor detection and surgical resection of ovarian cancer. The LAN-beta gal chemosensor shows potential as a new tool for the clinical diagnosis and treatment of ovarian cancer.
ANALYTICAL CHEMISTRY
(2023)
Article
Agricultural Engineering
Zhen Hu, Qian Li, Yuanyuan Chen, Tianqi Li, Youmei Wang, Ran Zhang, Hao Peng, Hailang Wang, Yanting Wang, Jingfeng Tang, Muhammad Nauman Aftab, Liangcai Peng
Summary: In this study, optimal ultrasound pretreatment was used to extract lignin and hemicellulose from recalcitrance-reduced rice mutant straw to improve cellulose accessibility. Intermittent ultrasound-assistant enzymatic hydrolyses were then conducted to increase hexose yield and bioethanol concentration. This study also generated biochar with high porosity and strong adsorption capacity for dyes. Therefore, this research demonstrates a green process technology for high-yield bioethanol and high-porosity biochar from lignocellulose.
BIORESOURCE TECHNOLOGY
(2023)
Article
Endocrinology & Metabolism
Yu-Fang Liu, Zi-Yi Liu, Wen-Tao Li, Peng Wang, Xiang-Yu Wang, Ran Di, Xiao-Yun He, Ming-Xing Chu
Summary: The aim of this study was to investigate the molecular mechanisms underlying MLT regulation of autophagy in GCs with different Fec(B) genotypes. The researchers found that autophagy levels were significantly higher in GCs with Fec(B) BB genotype than in those with Fec(B)++ genotype. ATG2B was associated with cell autophagy and highly expressed in GCs with Fec(B) BB genotype. MLT could protect GCs by decreasing ROS levels, especially in GCs with Fec(B) ++ genotype.
JOURNAL OF PINEAL RESEARCH
(2023)
Article
Environmental Sciences
Yu Jin, Rui Yang, Yongduo Guan, Xiaochen Liu, JiaHui Fu, Xuesheng Liu, Juanjuan Qu
Summary: The study investigates the effects of plant growth-promoting rhizobacteria (PGPR) Enterobacter sp. Zm-123 and a heavy metal adsorption biomass spent mushroom substrate (SMS) on the growth promotion of rape and reduction of cadmium (Cd) toxicity in contaminated soil. Results show that all treatment groups significantly improve soil properties and decrease exchangeable Cd (SE-Cd) in the soil. Additionally, the treatments increase the biomass and chlorophyll content of rape, enhance antioxidant enzyme activities, reduce proline and malondialdehyde (MDA) accumulation, and decrease the Cd content in the edible parts of rape. Among the treatments, the semi-solid fermentation (SSF) group demonstrates the strongest effect.
JOURNAL OF SOILS AND SEDIMENTS
(2023)
Article
Oncology
Miao He, Sichen Liang, Hao Deng, Guo Zhang, Zhiqi Wang, Xin Yang, Yue Wang, Yi Li, Xiuli Sun, Jianliu Wang
Summary: This study compared the feasibility and diagnostic value of sentinel lymph node (SLN) mapping using carbon nanoparticles (CNPs) and indocyanine green (ICG) in endometrial cancer (EC). The results showed that the detection rate, sensitivity, and negative predictive value were similar between the two methods. The study suggested that CNPs could be an alternative to ICG for SLN mapping when near-infrared imaging equipment is lacking.
JOURNAL OF SURGICAL ONCOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Zhiqing Liu, Wei Li, Wenbo Sheng, Shiyu Liu, Rui Li, Qian Li, Danya Li, Shui Yu, Meng Li, Yongsheng Li, Xin Jia
Summary: In this study, a versatile solvent-mediated polymerization-induced self-assembly (PISA) strategy was developed for the direct synthesis of highly N-doped hierarchically porous carbon spheres with tunable meso-macroporous configuration. The introduction of intermolecular hydrogen bonds enhanced the interfacial interactions between block copolymers, oil droplets, and polyphenols. Different cosolvent systems were used to systematically manipulate the dominant hydrogen-bond-driven interactions, resulting in diverse porous structures. The hierarchically structured meso-macroporous N-doped carbon spheres exhibited tunable sphere sizes and mesopores and macropores, providing a new platform for the synthesis of well-defined porous materials.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Agriculture, Multidisciplinary
Yan Guo, Zhengwen Zhu, Jing Lv, Yumei Li, Jing Chen, Xiyao Cheng, Ning Li, Jidong Liu
Summary: By modifying the glycolytic pathway of Escherichia coli and optimizing the whole-cell reaction condition, the engineered strain achieved high-yield production of d-allulose from low-cost substrate. The cell growth inhibition was alleviated and d-allulose production increased by developing an ATP regeneration system for enhanced substrate phosphorylation. Fine-tuning of carbon flux and implementation of fed-batch co-substrate strategy further improved the d-allulose titer and conversion rate.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Review
Medicine, Research & Experimental
Yangdan Liu, Dongsheng Wen, Chiakang Ho, Li Yu, Danning Zheng, Steven O'Reilly, Ya Gao, Qingfeng Li, Yifan Zhang
Summary: Fibrosis, caused by excessive deposition of extracellular matrix, is a common and serious condition leading to organ failure and death. Recent advances in epigenetics have provided new insights into the mechanism of fibrogenesis and potential therapeutic strategies for organ fibrosis.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Computer Science, Artificial Intelligence
Fulan Qian, Kaili Qin, Hai Chen, Jie Chen, Shu Zhao, Peng Zhou, Yanping Zhang
Summary: The recommendation system helps users make rational decisions by assisting them in selecting satisfactory products and services. To enhance recommendation accuracy, social information has been incorporated into most methods in recent years. However, current social recommendations only consider the impact of historical items and social information on users. This study proposes a model called Utilizes the influence of multiple potential factors for Social Recommendation (UimSRec) to capture the influence of various potential factors on users, including consumption experience, life cycle, social consumption culture, and social roles. By simulating these influences in the form of latent semantics and associating them with user representation, UimSRec improves recommendation performance significantly through modeling diverse potential impact information and their relationships.
KNOWLEDGE AND INFORMATION SYSTEMS
(2023)
Article
Optics
Guoqing Pu, Runmin Liu, Hang Yang, Yongxin Xu, Weisheng Hu, Minglie Hu, Lilin Yi
Summary: A recurrent neural network-based Fourier decomposition method is proposed for fast and accurate modeling of femtosecond mode-locked fiber lasers. Generalization over different cavity settings is achieved through a proposed prior information feeding method. With GPU acceleration, the mean time for the AI model to infer 500 roundtrips is less than 0.1 s, which is approximately 146 times faster than the traditional method. This AI-enabled approach is promising to become a standard method for femtosecond laser modeling.
LASER & PHOTONICS REVIEWS
(2023)
Article
Geochemistry & Geophysics
Xiao Ran Zhang, Sun-Lin Chung, Azman A. Ghani, Rezal Rahmat, Yi-Ju Hsin, Hao-Yang Lee, Ping-Ping Liu, Jinyu Xi
Summary: By analyzing Borneo and its surrounding areas, researchers have found that the crust of Borneo shares similarities with northern Australia, suggesting a common origin and the splitting of Borneo from northern Australia. Recent investigations have challenged the current understanding of Gondwana dispersion and Asian accretion, shedding new light on Asian climate change and biogeographic diversity.
Article
Radiology, Nuclear Medicine & Medical Imaging
Vick Lau, Linfang Xiao, Yujiao Zhao, Shi Su, Ye Ding, Christopher Man, Xunda Wang, Anderson Tsang, Peng Cao, Gary K. K. Lau, Gilberto K. K. Leung, Alex T. L. Leong, Ed X. X. Wu
Summary: A computational approach is proposed to improve the image quality of ultralow-field (ULF) MRI through deep learning of high-resolution brain data. This approach yielded high-quality three-dimensional images at 0.055 T with isotropic 1.5-mm synthetic resolution and a total scan time under 20 minutes.
MAGNETIC RESONANCE IN MEDICINE
(2023)
Article
Materials Science, Multidisciplinary
Lu Han, Haiyue Miao, Mengying Ni, Guangzhen Zhao, Guang Zhu, Yanjiang Li, Guoliang Gao, Hailong Huang, Min Xu
Summary: Functional hydrogel electrolytes are essential for functional energy storage devices, and their performance relies on the functional groups and interactions within the polymer chain. This study successfully synthesized a self-healable and redox bromide-ion additive hydrogel electrolyte (SAM-Zn) using a natural polymer backbone. The SAM-Zn showed remarkable self-healing ability, excellent mechanical properties, and high energy density in Zn-ion hybrid supercapacitors. This work provides a simple strategy for combining self-healing properties and excellent electrochemical performance in hydrogel electrolytes.
Article
Medicine, General & Internal
Xue Gao, Juan Zhao, Guan-Hua Li, Xi Wang, Wei Wang, Xing Liu, Min Liu, Meng-Meng Guo, Zhen-Dong Wang, Ya-Yan Lu, Jia Li, Yong Feng, Kun Yang, Jin-Cao Xu, Pu Dai
Summary: This study reports the case of a 28-month-old girl with congenital profound hearing loss, laryngomalacia, and malformed inner ear, who received cochlear implantation surgery. The genetic basis was determined using trio-based whole exome sequencing, and perioperative evaluation and management of the airway was performed by an experienced anesthesiologist. Cochlear implantation was eventually performed successfully under uneventful general anesthesia.
Article
Chemistry, Multidisciplinary
Yinghao Li, Xianqing Wang, Zhonglei He, Melissa Johnson, A. Sigen, Irene Lara-Saez, Jing Lyu, Wenxin Wang
Summary: The topological structures of polymers are crucial for determining their efficiency in gene delivery. This study synthesized a new generation of multi-cyclic poly(& beta;-amino ester)s (CPAEs) with unique topology structure for the first time via step growth polymerization. Through controlling the occurrence stage of cyclization, three types of CPAEs with rings of different sizes and topologies were obtained. In vitro experiments showed that the CPAEs with macro rings (MCPAEs) significantly enhanced transgene expression compared to their branched counterparts. Additionally, the MCPAE vector with optimized terminal group efficiently delivered the CRISPR plasmid encoding Staphylococcus aureus Cas9 nuclease and dual guide sgRNAs for gene editing therapy.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)